Labshake search
Citations for Qiagen :
251 - 300 of 10000+ citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: The cDNA for qRT experiments was prepared with miScript II RT kit (Qiagen, 218161) using HiFlex buffer ...
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bioRxiv - Microbiology 2020Quote: ... The total RNA was reverse transcribed into cDNA with miScript II RT Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 1μg of total RNA was reverse transcribed using the miScript II RT Kit (Qiagen). For qRT-PCR analysis of miRNAs ...
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bioRxiv - Microbiology 2019Quote: ... and EcoRI/XhoI digested pGREG533 was gel purified using a Qiaex II kit (Qiagen). The effector genes were cloned in-frame with the N-terminal 7× hemagglutinin tag (7-HA ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was purified from each supernatant using the QIAEX II Gel extraction kit (Qiagen) following the manufacturer’s protocol for concentrating DNA from solutions ...
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bioRxiv - Immunology 2020Quote: ... cDNA was synthesised from 500 ng RNA using the miScript II RT kit (Qiagen) with HiFlex buffer ...
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bioRxiv - Immunology 2020Quote: All constructs were purified from the agarose gel (QIAEX II Gel Extraction Kit, Qiagen), and digested by NotI and Bgl-II (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... and cDNA was synthesized using the miScript II RT Kit (Qiagen, 218160, California, USA) following the manufacturer’s instructions and stored at −80°C until use ...
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bioRxiv - Cell Biology 2021Quote: ... 250 or 500 ng of total RNA using the miScript II RT kit (Qiagen) and the cDNA was amplified with miScript SYBR Green PCR (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... the DNA was purified by using a QIAEX II Gel Extraction Kit (Qiagen, 20051). For PCR 2 ...
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bioRxiv - Developmental Biology 2023Quote: ... The extracted RNA was retro-transcribed using the miScript II RT kit (QIagen, 218161). qPCR for miR quantification was performed with miScript SYBR Green PCR kit (QIagen ...
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bioRxiv - Molecular Biology 2023Quote: ... and the products was purified with QIAEX II Gel Extraction Kit (Qiagen, Hilden, Germany). The labelled probes (200 ng ...
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bioRxiv - Molecular Biology 2024Quote: ... One µg of RNA was reverse transcribed using the miRScript II kit (218161; Qiagen) or miRCURY LNA RT Kit (339340 ...
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bioRxiv - Biophysics 2024Quote: ... Site-directed mutagenesis was performed using the Quickchange II XL Mutagenesis Kit (QIAGEN Sciences) for mutations in KCNQ1 ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR product was gel purified using QIAEX II gel extraction kit (QIAGEN, #20021). BP recombination (Invitrogren “Gateway BP Clonase II Enzyme Mix” #11789-020 ...
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bioRxiv - Plant Biology 2020Quote: ... coli cells grown overnight at 30°C (Plasmid Mini Kit, QIAGEN), and its concentration measured by nanodrop ...
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bioRxiv - Microbiology 2022Quote: ... at −80 °C using the RNeasy plus Mini Kit (Qiagen, 74134) according to the manufacturers protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli C mutants was extracted using DNeasy Blood & Tissue kit (Qiagen). Quality of extracted DNA was confirmed before sequencing ...
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bioRxiv - Genetics 2023Quote: Genomic DNA and RNA were extracted from each brain region using the All-Prep DNA/RNA/miRNA Universal kit (Qiagen Sciences Inc., Germantown, MD) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA from the control human TSCs as well as GATA2-KD and GATA3-KD human TSCs were isolated using RNeasy Mini Kit (Qiagen, Germantown, MD) per the manufacturer’s protocol with on-column deoxyribonuclease digestion ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339, human METTL3: QIAGEN SI05020414 and human DDX6 ...
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bioRxiv - Genetics 2019Quote: ... Ligation products were size-selected (250-300 bp) and purified from a 2% low-melting agarose gel using the MinElute Gel Extraction Kit (Qiagen). Ampure XP beads (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted at low speed (~1000 RPM) and lysed with one mL RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen).
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bioRxiv - Neuroscience 2022Quote: ... The heavy-chain specific amplicon was isolated using electrophoresis with low-melting point agarose extraction with the QIAquick Gel Extraction kit (Qiagen). A secondary amplification was performed using a modification of the primers (VHH-Esp-For ...
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bioRxiv - Microbiology 2023Quote: ... Stocks were passaged once at low multiplicity on MDCK-SIAT1 cells and RNA was extracted using the QIAmp Viral RNA mini kit (Qiagen). Genomic segments encoding the HA and NA proteins were amplified by reverse transcription polymerase (RT-PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... For tissues or isolated hepatocytes with high-fat and low-fat content the total RNA was obtained using a RNeasy Mini Kit (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: RNA from frozen FC area 8 was extracted following the supplier’s instructions (RNeasy Mini Kit, Qiagen® GmbH, Hilden, Germany). RNA integrity and 28S/18S ratios were determined with the Agilent Bioanalyzer (Agilent Technologies Inc ...
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bioRxiv - Genomics 2019Quote: ... and human genomic DNA (extracted from cells with DNeasy Blood & Tissue Kit, Qiagen). One guide was used with the plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared using the QIASeq Human Comprehensive Cancer Panel Kit (Qiagen #333515) and sequenced on an Illumina HiSeq 2500 or NovaSeq 6000 ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from human cells with an RNeasy Mini kit (Qiagen). Northern blot was performed as described previously (Tafforeau et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was extracted from human thyroid tissues using miRNeasy Mini kit (Qiagen) according to the manufacturer’s instructions and as described [26] ...
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bioRxiv - Neuroscience 2023Quote: ... Human rRNA depletion was performed using the QIASeq FastSelect kit (Qiagen, Hilden, Germany). RNA sequencing library preparation used NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s recommendations (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: DNA from human stool samples was extracted with DNeasy PowerSoil Pro kit (Qiagen) according to the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2022Quote: Total RNA for human TSCs was extracted using RNeasy Micro kit (Qiagen, 74004) with on-column DNase digestion ...
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bioRxiv - Synthetic Biology 2023Quote: ... or primary human cells were extracted using the Qiagen RNeasy Micro Kit (Qiagen). cDNA was produced using the SuperScript IV VILO cDNA Synthesis Kit (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... according to manufacturer’s protocol (MiScript Primer assays and II RT kit for cDNA synthesis and MiScript SYBR Green PCR Kit for RT-qPCR, 218161, Qiagen) from which the recovery of cel-miR-39 spike-in control was confirmed.
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bioRxiv - Molecular Biology 2021Quote: ... reverse transcription and quantitative real-time PCR (qRT-PCR) were performed with miScript II RT Kit and SYBR Green PCR Kit respectively (QIAGEN). To quantify α-Syn mRNA levels ...
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bioRxiv - Cancer Biology 2023Quote: ... was reverse transcribed into cDNA by miScript RT II Kit using miScript HiSpec Buffer or miRCURY LNA RT Kit (Qiagen). Expression levels of miR-142-5p were analyzed using miScript PCR System or miRCURY LNA SYBR Green PCR Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2023Quote: ... The RNA was extracted using the NucleoSpin RNA II kit (Machery & Nagel) and the cDNA were synthesized using the QuantiTect Reverse Transcription Kit (Qiagen). cDNA samples were run in triplicates and experiments were repeated with independently isolated RNA samples ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pH 5.8) under long day condtions (16h light) at 24 °C (day) 22 °C (night) using the DNeasy Plant Kit (Qiagen). ONSEN copy numbers were determined by qPCR using 12 ng total DNA using the KAPA SYBR FAST master mix universal on a C1000 Touch (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNAs were purified from 3 mL overnight cultures grew at optimum temperature (30 °C or 37 °C) by Dneasy Blood & Tissue Kits (QIAGEN). Additionally ...
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bioRxiv - Microbiology 2023Quote: ... Eluates were then treated with Proteinase K (45°C, 1400rpm, 4h) and RNaseA (37°C, 1400rpm, 30min) before cleanup using MiniElute PCR Purification kit (Qiagen). Purified DNA together with input samples before immunoprecipitation were amplified using qPCR SyBr green mix (PCR Biosystems ...
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... The TbHsp70 coding region was inserted into the pQE2 expression vector (Qiagen, U.S.A.) using NdeI and HindIII restriction sites ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... we prepared total RNA from dissected wildtype mouse brain regions using Qiazol (Qiagen) and prepared cDNA using the SuperScript III first-strand synthesis kit (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... After 24 hours the media was removed for ELISA and the cells were lysed and RNA extracted (RNeasy Mini Kit, Qiagen).
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bioRxiv - Microbiology 2019Quote: ... TissueLyser II (Qiagen) was further used to break heterocysts at frequency of 30/s for 8 min ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339 ...
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bioRxiv - Immunology 2021Quote: ... All PCR products were purified with the QIAEX II Gel Extraction Kit (Qiagen, Hilden, Germany) prior to subsequent cloning ...
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bioRxiv - Genomics 2020Quote: ... DNA was purified using the QIAEX II Gel Extraction Kit (Cat # 20021, Qiagen, Hilden, Germany). Mouse genomic DNA was purified from liver ...
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bioRxiv - Molecular Biology 2021Quote: ... and mono-nucleosomal DNAs were extracted using a QIAEX II gel extraction kit (20021, Qiagen) according to the manufacturer’s manual ...