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Citations for Qiagen :
601 - 650 of 1798 citations for Human DOK4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Plasmids were purified using QIAprep spin miniprep kit (Qiagen). Oligonucleotides used for gene cloning or screening purposes were purchased from Sigma and their sequences are listed in the S3 Table ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid and DNA purification kits were purchased from Qiagen and Agilent ...
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bioRxiv - Genomics 2023Quote: ... The plasmid was amplified and purified via Maxiprep (QIAGEN). Both TRE-RNASEH1ΔMLS-BFP and BFP-only control vectors were verified by Sanger sequencing at GENEWIZ.
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bioRxiv - Genetics 2023Quote: ... plasmids were isolated using Qiaprep spin miniprep kit (Qiagen).
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bioRxiv - Developmental Biology 2022Quote: ... plasmids were extracted using Miniprep kit (Qiagen, Düsseldorf, Germany). Plasmids were linearized and probes were prepared as described in Wilkinson & Nieto (1993 ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids were isolated using a Spin Miniprep kit (Qiagen). Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were extracted with Qiagen miniprep kits (Qiagen GmbH) and verified via Sanger sequencing (Source Biosciences) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA isolations were performed using plasmid Miniprep kit (Qiagen). All isolations were performed according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: QIAGEN Plasmid Plus Midi Kit (QIAGEN, cat. No. 12945)
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bioRxiv - Cell Biology 2023Quote: ... A maxiprep was performed to purify the plasmid (Qiagen), and the plasmid subsequently used for stable cell line generation via lentiviral transduction.
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bioRxiv - Cancer Biology 2023Quote: ... and 900ng lentiviral plasmid backbone using PolyFect (Qiagen, 301105) into HEK293t cells in 6-well format according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Expression plasmids were transfected using Effectene transfection reagent (QIAGEN). Plasmids were generated via Gateway® technology (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were isolated using QIAprep Spin Miniprep kits (Qiagen) according to the Miraprep protocol (Pronobis et al. ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were purified by using Spin Miniprep Kit (Qiagen). Sequencing (Eurofins Genomics ...
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bioRxiv - Microbiology 2024Quote: ... CUBES01 using a Plasmid Mini Kit (QIAGEN, Hilden, Germany) for screening on the presence of the respective plasmids ...
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bioRxiv - Biochemistry 2023Quote: ... MN (Germany) or Plasmid Midi kits from Qiagen (Germany).
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bioRxiv - Cell Biology 2019Quote: ... Human primers were pre-validated Quantitect primers (Qiagen, Manchester, UK). Comparative quantification normalised target gene mRNA to β-actin (ACTB ...
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bioRxiv - Microbiology 2022Quote: ... DNA from human samples was extracted with PowerSoil Pro (Qiagen) on the QiaCube HT (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... the human Stress & Toxicity PathwayFinder RT2 Profiler™ technology (Qiagen), assessing expression of 84 stress response-related genes ...
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bioRxiv - Immunology 2020Quote: ... Mouse and Human IFN I RT2 Profiler PCR Arrays (Qiagen) were performed and relative expression determined using the ∆∆CT method and normalized for 5 housekeeping genes according to manufacturer’s guidance.
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bioRxiv - Cancer Biology 2022Quote: ... individual Human SHANK3 siRNA_2 (Cat. no. S100717710 Hs_SHANK3_2 siRNA, Qiagen) and individual ON-TARGETplus Human SHANK3 siRNA_7 (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... and Human Serum/Plasma miRCURY LNA miRNA PCR array (Qiagen, Catalog Number ...
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bioRxiv - Immunology 2023Quote: ... RNA from human PBMCs was isolated using RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: A siRNA library targeting all human kinases (Qiagen, Hilden, Germany) was utilized ...
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bioRxiv - Molecular Biology 2021Quote: ... All constructs were sequenced before use and plasmids used for transfection experiments were purified with the EndoFree® plasmid maxi kit (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2020Quote: ... The ligated cDNA was transformed into XL10-gold and the plasmids were purified using a plasmid plus midi kit (Qiagen, Germantown, MD, USA). The complexity of the cDNA library was 2 × 106 ...
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bioRxiv - Microbiology 2023Quote: ... approximately 1 x 104 colonies were collected and pooled into LB-amp culture media and then incubated at 37°C for five hours prior to harvesting the bacterial population for plasmid purification by Maxiprep (Qiagen Plasmid Maxi Kit). The presence of a diverse barcode in the plasmid stock was verified by next generation sequencing (see below) ...
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bioRxiv - Microbiology 2023Quote: ... bacterial colonies were pooled into LB-ampicillin culture media and amplified followed by plasmid purification with the Plasmid Maxi Kit (Qiagen, Cat. No. 12165). Barcode diversity in the plasmid stock was confirmed via next-generation sequencing (Amplicon-EZ – Azenta Life Sciences) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 ng of pFLAG expression vectors or empty plasmids and 50 ng psiCheck-2 plasmid were transfected using Effectene transfection reagent (Qiagen, Netherlands, Cat. No. 301425), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were purified using QIAprep Spin Miniprep kit (Qiagen) for Sanger sequencing (Macrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was isolated (QIAprep Spin Miniprep Kit, Qiagen, 27104) from each transformant and subsequently 5 μL plasmid DNA (80-100 ng/μL ...
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bioRxiv - Biophysics 2022Quote: ... After purification using a Qiagen Maxiprep plasmid purification kit (Qiagen). The 64x repeat was then cleaved out of the plasmid using XbaI ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmids were extracted and purified using a Miniprep Kit (QIAGEN), and sequenced (GENEWIZ) ...
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bioRxiv - Developmental Biology 2019Quote: ... using 2µg of plasmid DNA and Polyfect transfection reagent (Qiagen). Positive selection was performed by adding 25µg/ml of hygromycin B (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were prepared for injection using standard miniprep extraction (Qiagen) and submitted for injection (Microinjection service ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmid DNA was purified using QuickLyse Miniprep Kit (Qiagen, USA). The construct containing OGR1 was further confirmed by sequencing using the ABI Prism 377 Automated DNA Sequencer (Applied Biosystems ...
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bioRxiv - Physiology 2019Quote: ... plasmids were extracted using the QIAprep Spin Miniprep kit (Qiagen) and sequenced by ABI 3730 automated DNA sequencer at Davis Sequencing (Davis ...
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bioRxiv - Cell Biology 2019Quote: Plasmids were transfected for 22 hours using Effectene (Qiagen, Germany) according to the manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN).
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bioRxiv - Genomics 2019Quote: ... We miniprepped plasmids using the Qiaprep Spin Mini kit (Qiagen) and verified plasmid sequences through Sanger sequencing with the RV3 primer.
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bioRxiv - Genetics 2019Quote: ... DNA maxiprep kit was from Qiagen (EndoFree Plasmid Maxi Kit). All plasmids were cloned with Gibson assembly (42 ...
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bioRxiv - Cancer Biology 2020Quote: ... The phagemid DNA was isolated using plasmid miniprep kit (Qiagen).
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bioRxiv - Bioengineering 2021Quote: ... dsDNA was extracted using the Plasmid Miniprep Kit (Qiagen, 27104). dsDNA concentration was measured using a Nanodrop (Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... Plasmids were purified using the QIAprep spin miniprep kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... Plasmid DNAs were initially isolated using a maxiprep kit (Qiagen). To remove nicked plasmid species ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected in U87 cells with Effectene (Qiagen, Germany) according to the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2021Quote: ... isolated with the use of a Plasmid Maxi Kit (Qiagen), and then subjected to in vitro transcription in a transcription mixture of 100 μl containing 10 μg of plasmid as template DNA ...
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bioRxiv - Biochemistry 2020Quote: ... The vector was isolated using Qiagen plasmid maxi kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmid and DNA fragments were purified using kits from Qiagen according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... or the pEhEx plasmid backbone using Attractene transfection reagent (Qiagen). Stable transfectants were initially selected at 3 µg/ml Geneticin (ThermoFisher Scientific) ...