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Citations for Qiagen :
451 - 500 of 10000+ citations for Human 60S ribosomal protein L22 like 1 RPL22L1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... rad54-1 and rad54-2 plants using RNeasy Plant mini Kit (QIAGEN), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... for less than 1×104 cells or the RNeasy kit (Qiagen #74004) otherwise ...
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bioRxiv - Microbiology 2020Quote: ... Each 1-kb fragment was purified using a gel extraction kit (Qiagen) and were ligated by PCR using primers Lpg499-DR and Lpg499-UF to generate a 3-kb fragment that was purified by a gel extraction kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... multivorans 1 genomic DNA (gDNA, extracted with DNeasy Blood & Tissue Kit, Qiagen), cleaned up using the Monarch PCR Purification kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... amplicons were subjected to the QIAseq 1-Step Amplicon Library Kit (Qiagen) for Illumina compatible next generation sequencing (NGS ...
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bioRxiv - Genomics 2020Quote: ... and purified from a 1% agarose gel (QIAquick Gel Extraction Kit, Qiagen).
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 1 × 106 cells using the RNeasy kit (Qiagen), and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: THP-1 and primary macrophage RNA was extracted using RNeasy kit (Qiagen), followed by cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using QuantiNova SYBR Green 1 Step kit (Qiagen) with 40 cycles of amplification at 57ᵒC performed on an ABI 7500 (Applied Biosystems) ...
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bioRxiv - Genomics 2020Quote: ... Protein was precipitated by adding 200 µL of ice-cold Protein Precipitation Solution (Qiagen), gentle mixing and incubation on ice for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The peptide-coupled proteins were separated from uncoupled proteins using Ni-NTA Agarose (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... N-terminal His-tagged proteins were purified using a QIAexpress protein purification system (Qiagen), as previously described47.
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bioRxiv - Biophysics 2021Quote: ... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
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bioRxiv - Biochemistry 2023Quote: Total RNA was isolated and purified from cultured cells using a standard commercial RNA prep kit (#48500, Norgen) and 1 μg of RNA was reverse transcribed to cDNA using a cDNA synthesis kit (#205314, Qiagen). 10 ng of cDNA was used for quantitative PCR by SYBR Green ...
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bioRxiv - Developmental Biology 2022Quote: ... 333µl Protein Precipitation Solution (Qiagen) was added and samples were vortexed vigorously for 20 seconds at high speed ...
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bioRxiv - Cell Biology 2023Quote: ... Protein Precipitation Solution (Qiagen #158910), DNA Hydration Solution (Qiagen #158914 ...
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bioRxiv - Biochemistry 2021Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Molecular Biology 2021Quote: The solubilized protein was incubated for 1 hour with washed and pre-equilibrated Strep-Tactin Superflow plus resin (Qiagen). The resin was then loaded into an Econo-Column Chromatography Column (Biorad ...
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bioRxiv - Biochemistry 2020Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...
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bioRxiv - Cell Biology 2019Quote: ... Human primers were pre-validated Quantitect primers (Qiagen, Manchester, UK). Comparative quantification normalised target gene mRNA to β-actin (ACTB ...
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bioRxiv - Microbiology 2022Quote: ... DNA from human samples was extracted with PowerSoil Pro (Qiagen) on the QiaCube HT (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... the human Stress & Toxicity PathwayFinder RT2 Profiler™ technology (Qiagen), assessing expression of 84 stress response-related genes ...
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bioRxiv - Immunology 2020Quote: ... Mouse and Human IFN I RT2 Profiler PCR Arrays (Qiagen) were performed and relative expression determined using the ∆∆CT method and normalized for 5 housekeeping genes according to manufacturer’s guidance.
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bioRxiv - Cancer Biology 2022Quote: ... individual Human SHANK3 siRNA_2 (Cat. no. S100717710 Hs_SHANK3_2 siRNA, Qiagen) and individual ON-TARGETplus Human SHANK3 siRNA_7 (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... and Human Serum/Plasma miRCURY LNA miRNA PCR array (Qiagen, Catalog Number ...
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bioRxiv - Molecular Biology 2023Quote: A siRNA library targeting all human kinases (Qiagen, Hilden, Germany) was utilized ...
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bioRxiv - Immunology 2023Quote: Total DNA and mRNA were isolated from the intestine samples collected during fish necropsy with the AllPrep DNA/RNA/Protein Kit (Qiagen, Hilden, Germany), following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Samples were then centrifuged and plasma isolated and stored at -20°C prior to testing in the QuantiFERON IFNγ ELISA (Qiagen). Acceptance criteria for the assay specified by manufacturers were always met.
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bioRxiv - Plant Biology 2022Quote: RNA was extracted from 10-day-old whole seedlings grown on 1/2X MS media containing 1% sucrose and 0.8% agar (Plant RNeasy kit (Qiagen)) ...
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bioRxiv - Cell Biology 2020Quote: ... For each replicate about 60 wing discs were dissected from wandering larvae and immediately transferred to the lysis buffer (Buffer RLT, Qiagen) supplemented with β-mercaptoethanol (1:100 ...
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bioRxiv - Molecular Biology 2021Quote: Cells were transfected at 60-80% confluence with 5 nM/1nM (MIN6, EndoCβ-H1, respectively) control or miR-125b mimics (Qiagen) or 50 nM of a mixture of four ONTARGETplus siRNAs against mouse Smad2 ...
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bioRxiv - Biophysics 2022Quote: ... Purified protein was diluted in PBS buffer (pH = 7.2) and the fluorescence intensity was recorded at 60 °C in the Rotor-Gene 6600 real-time PCR cycler (Qiagen) for 18 h ...
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bioRxiv - Molecular Biology 2019Quote: ... An aliquot of the cleared lysate (60 µl) was saved as input and the remaining volume was added to 80µl of Ni-NTA beads (Qiagen 36111) pre-equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... the manufacturer’s directions were followed with the exception of adding 500ul of 10% SDS (w/w) to the homogenate at 60°C for 20 minutes before the addition of P2 buffer (Qiagen). This allowed the brain tissue to better solubilize with the Qiagen buffers ...
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bioRxiv - Developmental Biology 2020Quote: ... at a density of 1-2×106 cells/ml and transfected with pRmHA3-GAL4 [60] and HA-tagged p10UAST-Robo or untagged pUAST-Slit plasmids using Effectene transfection reagent (Qiagen). GAL4 expression was induced with 0.5 mM CuSO4 for 24 hours ...
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bioRxiv - Biochemistry 2020Quote: ... 80 µL of the mixture was then incubated for 45-60 minutes at room temperature or 4°C with 10 µL NiNTA resin (Qiagen) prewashed with His-SUMO Lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: The resulting sequencing data was analyzed by mapping reads to a database containing 60 bases of sequence flanking each secondary gene using CLC Genomics Workbench (Qiagen). Total read counts at each locus were normalized to the secondary gene with the greatest number of reads in each sample ...
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bioRxiv - Immunology 2022Quote: ... Samples were centrifuged once more and RNA pellets were air-dried for 30-60 minutes at room temperature before resuspending in 30 μl RNase-free water (Qiagen). The quality and quantity of RNA was measured using a NanoDrop Microvolume spectrophotometer ...
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bioRxiv - Cancer Biology 2022Quote: ... and protein extraction were performed according to manufacturer instructions (AllPrep DNA/RNA/Protein minikit; Qiagen). Each spin column flowthrough (DNA ...
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bioRxiv - Microbiology 2023Quote: ... The His-tagged proteins were purified using the QIAexpress Ni-NTA Protein Purification System (Qiagen) with 0.1 M ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from the cortical brain and cochlea samples from 1-month-old mice using RNA extraction kit and Qiazol Reagent (RNeasy mini kit; Qiagen, Germany), and DNase treatment was performed (RNase-free ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg of genomic DNA extracted using the DNeasy Blood & Tissue kit(Qiagen) was bisulfite-converted by using the EZ DNA Methylation kit (Zymo Research) ...
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bioRxiv - Immunology 2019Quote: Total RNA was extracted from 1 × 107 PBMC using RNeasy mini kit (Qiagen, Hilden ...
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bioRxiv - Plant Biology 2020Quote: ... bron-1 and bron-2 root tips using the RNeasy Micro Kit (Qiagen). qPCR was performed with SYBR green (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of total RNA was retrotranscribed using QuantiTect Reverse Transcription kit (Qiagen). Primers designed to amplify cpxP ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of total RNA extracted with the RNeasy Plant Mini Kit (Qiagen) was treated with DNaseI (Ambion ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 µg total RNA was reverse transcribed using the Quantitect kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA (1 μg) was reverse-transcribed by QuantiTect Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 200ul and 600ul Buffer RLT (containing 1% β-mercaptoethanol, QIAGEN RNeasy Mini Kit) was added to nuclear and cytosolic fractions respectively and vortexed vigorously ...