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Citations for Qiagen :
401 - 450 of 948 citations for Hepatitis C Virus Core Antigen HCcAg since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... was stored in Qiazol at −80°C for later RNA extraction (Qiagen, Hilden, Germany). DNA was extracted using the DNeasy Blood & Tissue Kit including the optional RNase A treatment (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: Library preparation using QIAseq Stranded mRNA Lib Kit UDI-C (96) (Qiagen, Cat. # 180443) and paired-end sequencing on Illumina Hiseq X Ten were done by Biokart India Pvt ...
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bioRxiv - Neuroscience 2023Quote: ... and tissue was homogenized at 4°C in a tissue lyser bead mill (Qiagen) for 2 min at 20 Hz ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 minutes at 37°C and purified with Minelute PCR Purification Kit (Qiagen) to produce tagmented DNA samples ...
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bioRxiv - Biophysics 2023Quote: ... 4 °C and the supernatant was loaded onto a NiNTA column (Qiagen, Hilden, Germany) previously equilibrated with washing buffer (20 mM Tris pH 8 ...
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bioRxiv - Biophysics 2023Quote: ... for 4 h at 37 °C and purified with RNeasy Mini Kit (Qiagen; #74104). Following the in vitro transcription ...
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bioRxiv - Immunology 2023Quote: ... Larvae were euthanized at 4°C overnight and homogenized with a tissue lyser (Qiagen) at 1800 oscillations/min (30 Hz ...
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bioRxiv - Plant Biology 2020Quote: ... for 30 min at 37°C and cleaned using the RNeasy Plant Mini kit (Qiagen). The quality and quantity of RNA were assayed using an Agilent RNA 6000 Nano Kit Bioanalyzer chip (Agilent Technologies) ...
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Chromatin accessibility changes at intergenic regions associates with ovarian cancer drug resistancebioRxiv - Cancer Biology 2021Quote: ... at 37°C for 10 minutes before purification using the MinElute PCR cleanup kit (Qiagen). Isolated DNA was run on TapeStation High Sensitivity DNA tape (Agilent) ...
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bioRxiv - Molecular Biology 2021Quote: ... for 15 minutes at 37°C and then purified using QIAquick PCR Purification Kit (Qiagen). Inserts were ligated into vectors using Golden Gate assembly ...
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bioRxiv - Microbiology 2020Quote: ... for 30 min at 37°C before purification with the RNeasy MiniElute Cleanup kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Testis tissue was homogenized at 4°C in 1ml QIAzol Lysis Reagent (Qiagen, Hilden, Germany) together with RNase-Free Zirconium Oxide Beads (NextAdvance ...
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bioRxiv - Biophysics 2020Quote: ... 4 °C) and the supernatant was then incubated with 200 µl Ni-NTA (#30230, Qiagen) beads for 1 hour at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 37°C overnight followed by plasmid DNA purification using a HiSpeed Plasmid Midi Kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... 37°C overnight followed by plasmid DNA purification using a QIAprep Spin Miniprep Kit (Qiagen).
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bioRxiv - Cell Biology 2021Quote: ... 4 °C) and the supernatant was then loaded onto 1 ml HisTrap HP column (Qiagen) at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... for 6 h at 55 °C followed by purification with a Qiaquick PCR Kit (Qiagen). Libraries were prepared using a MicroPlex Library Preparation Kit (Diagenode ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was then incubated for one hour at 4 °C with Ni-NTA (Qiagen) in batch adsorption format ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was incubated for 2 h at 4 °C with Ni2+-NTA-agarose (Qiagen) (20 mg of proteins/ml of resin ...
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bioRxiv - Microbiology 2021Quote: ... and stored at −80 °C before extraction using RNeasy with on column DNase treatment (Qiagen). Ribosomal RNA was depleted using Ribozero (Illumina ...
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Variation in Leishmania chemokine suppression driven by diversification of the GP63 virulence factorbioRxiv - Microbiology 2021Quote: ... and stored at -80°C prior to extracting RNA with RNeasy RNA extraction kit (Qiagen). Reverse transcriptase was performed using iScript Reverse Transcriptase kit (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... Purification of C-terminal His6-tag-labeled GluN1 and GluN1ΔM4 by Ni2+-NTA agarose (Qiagen) chromatography was performed as in (Madry et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... 25 minutes incubation at 37°c was given with RNase free 2U DNase (Qiagen-79254) in RDD buffer to remove residual DNA ...
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bioRxiv - Microbiology 2019Quote: ... at 37°C for 30 min and were re-purified using RNeasy mini kit (Qiagen). RNA concentrations were determined using Nanodrop ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was incubated for 1 hour at 4°C with Ni-NTA-agarose (Qiagen), washed several times with lysis buffer and the protein was eluted by incubation for 3 hours with lysis buffer containing 500 mM imidazol ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by enzyme inactivation 20 minutes at 65°C and column-based purification (Qiagen, #74204). First strand cDNA synthesis was performed with the High-Capacity-RNA-to-cDNA Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated for 3 minutes at 56°C in a deparaffinization solution (Qiagen, 19093). A step of digestion with Proteinase K was performed followed by a treatment with DNAses ...
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bioRxiv - Neuroscience 2023Quote: ... The eluent was then incubated overnight at 4°C with Ni-NTA beads (Qiagen, 36111), which were subsequently collected ...
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bioRxiv - Cell Biology 2023Quote: ... for 30 minutes at 37°C and purified with Minelute PCR Purification Kit (Qiagen, 28004) to produce tagmented DNA samples ...
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bioRxiv - Immunology 2023Quote: ... Sorted Ly6G+ cells were stored at −80°C in RLT Plus Buffer (Cat# 1053393-Qiagen). Total RNA isolation was done using RNeasy Plus Mini Kit (Cat# 74134-Qiagen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Light organs were then transferred into 180 ul of pre-heated (98°C) ATL buffer (QIAGEN) and incubated at 98°C for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... incubated for 45min at 42°C and the reaction purified with 15µl of MagAttract beads (Qiagen). The labelled DNA was eluted with TE-buffer (10mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: An RNA probe targeting c-myb was generated using one-step RT-PCR (Qiagen, Manchester, UK) using the following primers c=myb-F 5’-CCAAGTCAGGAAAACGCCACCTCG-3’ and c-myb-R 5’-GCTGTTGTTTAGCGGAGTTGGGCT-3’ and cloned into the dual promoter vector pCRII-TOPO (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... then spun at 14000K RPM for 10 minutes at 4°C to pellet debris (Qiagen, 37901). Protein concentration in the supernatant was quantified using BCA assay (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 4°C) and total RNA was extracted from the supernatant using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... for 20 min at 37°C followed by purification with the QIAquick PCR purification kit (Qiagen). To quantify the RNA transcripts ...
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bioRxiv - Biophysics 2020Quote: ... Enzyme was inactivated (65°C, 15min) prior to PCR purification of the linearized product (Qiagen, Germany). 10μg of linearized plasmid was transformed into Pichia pastoris X-33 strain by electroporation as per the manufacturers protocol (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) and supernatant was transferred to tubes containing 30µl precleared Ni-NTA agarose beads (Qiagen) in the presence of 0.05% Tween-20 and 15mM imidazole ...
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bioRxiv - Genomics 2021Quote: Skeletal muscle previously stored at −80°C was lysed with the RLT buffer Plus buffer (Qiagen) and beta-mercaptoethanol using the TissueLyser II (Qiagen ...
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bioRxiv - Developmental Biology 2020Quote: ... for 20 min at 37°C and purification with the RNeasy MinElute Cleanup kit (Qiagen, #74204). RNA quality was checked with the Agilent 2100 Bioanalyzer ...
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bioRxiv - Cell Biology 2021Quote: ... snap frozen (−80°C) for proteomics analysis or dissolved in 300 ul of RNAlater reagent (Qiagen).
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bioRxiv - Bioengineering 2021Quote: ... at 37°C for 30 min and further purified using an RNeasy Mini Kit (Qiagen, 74104) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 1 h at 55°C with 20 mg ml−1 proteinase K (Qiagen). Samples were treated for 10 min at 65 °C with 4 μl RNase A (100 mg ml−1 ...
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bioRxiv - Neuroscience 2021Quote: ... Hypothalamic blocks were dissected and stored at − 80°C until RNA extraction using RNAeasy kits (Qiagen).
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bioRxiv - Immunology 2022Quote: ... at 50 °C for 1 hour and were purified with a MinElute PCR Purification Kit (QIAGEN).
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bioRxiv - Molecular Biology 2022Quote: ... Further these lysates were incubated overnight at 4°C with prewashed Ni-NTA beads (Qiagen, 30210).Sequential washes with wash buffer 1-4 ...
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bioRxiv - Molecular Biology 2022Quote: ... rotated at 4°C for 1.5-2 hr in 0.75 mL equilibrated Ni-NTA Agarose (Qiagen), followed by packing pre-equilibrated Poly-Prep Chromatography Columns (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... 3 min in 37°C shaker) and processed with DNeasy Blood and Tissue Kit (QIAGEN #69504). Two hundred ng of genomic DNA was used as input in the DamID protocol that included an improved pool of AdR primers as described (de la Cruz Ruiz et al ...
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bioRxiv - Evolutionary Biology 2022Quote: ... tissues were incubated overnight at 56 °C in 300 μL of ATL digestion buffer (Qiagen®) including proteinase K (Cf = 1 mg/ mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were stored at -80°C with the addition of RNA protect reagent (Qiagen, MD, USA). DNA was extracted using DNeasy PowerSoil kit (Qiagen ...