Labshake search
Citations for Qiagen :
151 - 200 of 3061 citations for Glyphosate Chemical Purity 96% 2 13C 99%; 15N 98+% 100 Ug Ml In H2O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The column was prepared with 2 mL Ni-NTA Agarose Resin (Qiagen). The resin was equilibrated with 10 column volumes of column buffer and resuspended with the supernatant cell extract for 1 h at 4°C with orbital shaking ...
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bioRxiv - Microbiology 2024Quote: ... The cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen) on a rotator for 1 hour at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmids were transformed into JM109 chemically competent cells and grown overnight in 250 mL of LB broth with 100 µg/mL of ampicillin and purified using the HiSpeed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Synthetic Biology 2019Quote: ... grown over night in 5 mL LB and 100 μg mL-1 ampicillin and DNA were purified using a QIAprep Spin Miniprep Kit (QIAGEN). Sequencing was performed by GATC using the primers in SI Appendix Table S4 ...
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bioRxiv - Genetics 2019Quote: ... genomic DNA was extracted from a saturated culture of 100 ml under anaerobic condition (YPD) using the genomic tip-100 kit (Qiagen, Courtaboeuf, FRANCE). Paired-end Illumina sequencing libraries were prepared from sonicated genomic DNA according to manufacturer protocols (Genomic DNA Sample Preparation ...
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bioRxiv - Cell Biology 2022Quote: ... RNA purity and integrity were checked by microfluidic electrophoresis using the Bioanalyzer (Qiagen, Hilden Germany). Following the qualtity control procedure ...
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The gut hormone Allatostatin C regulates food intake and metabolic homeostasis under nutrient stressbioRxiv - Physiology 2020Quote: ... were homogenized in 2-mL tubes containing lysis buffer plus 1% 2-mercaptoethanol using a TissueLyser LT bead mill (Qiagen) and 5-mm stainless-steel beads (Qiagen #69989 ...
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bioRxiv - Microbiology 2021Quote: Swab RNA was extracted from 0.2 ml of swab solutions (1ml of DMEM with 2% fetal bovine serum [Cytiva]) using QIAamp Viral RNA Minikit (QIAGEN) and subjected to real-time RT-PCR for viral RNA quantitation [40] using QuantiTect Probe RT-PCR Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... RNA was eluted from the column in RNase/DNase free H2O and any residual DNA contamination was removed with a 1 hour off-column DNase treatment (Qiagen). The RNA was purified following the RNeasy Mini-Prep Kit following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 7 µL of RNase inhibitor and 98 µL nuclease-free water) and the eluted RNA was then concentrated using the RNeasy MinElute Cleanup kit (Qiagen, ref. 74204). The immunoprecipitated RNA was then reverse transcribed and used for qPCR ...
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bioRxiv - Genomics 2021Quote: ... counted and 2×104 were lysed in 100 µL of lysis buffer (RLT Plus (Qiagen, 1053393) and 1% ß-mercaptoethanol (Sigma-Aldrich,M3148)
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bioRxiv - Microbiology 2020Quote: ... 1 ug of RNA was reverse transcribed to cDNA with the QuantiTect reverse transcription kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was extracted from the red blood cells of the parents and 99 F2 hybrid females using the DNeasy Blood & Tissue Kit (Qiagen). The library was constructed according to a previously described method [27] ...
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bioRxiv - Cancer Biology 2022Quote: ... 15 μL of lysis buffer (Triton X-100 0.4%, Qiagen Protease 0.1 AU/mL) were added to each well and samples were incubated at 56 °C for 10 minutes and 72 °C for 20 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... 0.5% (v/v) Triton X-100) and 1 mg/ml Proteinase K (QIAGEN, Germany). The solution was incubated at 55°C for 15 min ...
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bioRxiv - Microbiology 2019Quote: ... or in a 96-well microplate format with the QIAamp 96 DNA Qiacube HT kit (Qiagen).
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bioRxiv - Genomics 2022Quote: ... equal volumes of the clones were combined together and the pool expanded in 20 mL of LB with 100 μg/mL carbenicillin for 9 hr followed by plasmid purification (Qiagen, 12943). Approximately 250 colonies in total were harvested.
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bioRxiv - Immunology 2022Quote: ... 65-79*SE or 65-79*PE (100 μg/mL) with or without IFN-γ (10 ng/mL) using the RNeasy mini kit (Qiagen) according to the vendor instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Spheroblasts were collected at RT and 2,500 g for 5 min and then re-suspended in 1.6 mL per 100 mL culture homemade buffer P1 (according to the manufacturers protocol, QIAGEN, Hilden, Germany). 1.6 mL per 100 mL culture of homemade buffer P2 (according to the manufacturers protocol ...
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bioRxiv - Microbiology 2023Quote: ... 3–20 μl of individual PCR products (adjusted on the basis of estimated relative amplicon concentration) were combined into 100 μl subpools and purified using an UltraClean 96 PCR cleanup kit (Qiagen Beverly LLC #12596-4). Despite not producing visible PCR bands ...
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bioRxiv - Synthetic Biology 2019Quote: ... where it was incubated with 2 mL] Ni-NTA agarose bead slurry (QIAGEN) for 10 min at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The cell lysate was applied on 2 ml of Ni-NTA agarose (QIAGEN) equilibrated with Buffer A ...
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bioRxiv - Microbiology 2020Quote: 2 □ 107 spores were mixed with 1 ml RNAlater RNA stabilization reagent (Qiagen) and centrifuged at 17,000 g for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... in a 2 mL centrifuge tube for 3 min using a TissueLyzer (Qiagen). The obtained mixture was serially diluted in 5.0 mL sterile phosphate buffer and 150 µL aliquots of 10−4 to 10−7 dilutions were spread onto plates containing a range of media ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 400 cotyledons were ground in 2 ml tubes using a Tissue Lyser (Qiagen) for 2 x 1 min at 30 Hz ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with buffer [50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with buffer [50 mM Tris-HCl ...
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bioRxiv - Genomics 2023Quote: ... The supernatant was mixed with 2 ml Ni-NTA agarose (Qiagen, no. 30250) and kept on a rotator at room temperature for 1 h.
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 2 mL of RNAprotect bacterial reagent (Qiagen; 76506) and incubated at room temperature for 5 minutes prior to storage at - 80°C until further processing ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatant was loaded onto 2 ml Ni-NTA Superflow resin (Qiagen). The resin was washed once with 10 mL resuspension buffer and three times with 10 mL resuspension buffer supplemented with 30 mM imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with buffer [50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was fully homogenized in 2 ml of QIAzol buffer reagent (Qiagen) on ice and Dounce homogenization ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with buffer [50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with buffer [50 mM Tris-HCl ...
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bioRxiv - Physiology 2023Quote: ... using QIAquant 96 2plex (QIAGEN, Germany). Relative expression was calculated using the 2-ΔΔCt method [58] ...
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bioRxiv - Microbiology 2023Quote: ... QIAprep 96 Turbo Miniprep kits (Qiagen) were used to isolate plasmids according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... approximately 25 mg of tissues were homogenized in 2 mL tubes containing 600 μL of Buffer RLT with 2% β-mercaptoethanol and a stainless steel bead (5 mm, Qiagen) using a TissueLyser II system (Qiagen) ...
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bioRxiv - Immunology 2019Quote: ... longitudinal muscle/myenteric plexus preparations were homogenized in a 2 ml eppendorf containing 1 ml Trizol and a single 5 mm steel bead in a TissueLyzer (Qiagen) for 3 min at 30 Hz ...
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bioRxiv - Microbiology 2021Quote: The supernatant was carefully removed to a 50-mL sterile tube and mixed with 2 mL volume of 50% Ni-NTA resin (Qiagen) which was pre-equilibrated in the buffer A ...
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bioRxiv - Microbiology 2021Quote: ... Mosquito bodies and legs and wings were put into a 2 ml round-bottom tubes containing 250 ml of PBS and a steel ball (Qiagen). Samples were homogenized using a TissueLyser (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... and early stationary phase (OD = 1.0-1.3).1 mL sample of each culture was directly transferred to 2 mL RNAprotect cell reagent (Qiagen, Hilden, Germany). The samples were vortexed 5 sec ...
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bioRxiv - Microbiology 2023Quote: ... Pellets were washed in 2 mL cold 10 mM NaCl + 4 mL cold RNAprotect Bacteria Reagent (Qiagen Cat. No. 76506) and repelletted at 4,255 x g ...
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bioRxiv - Microbiology 2019Quote: ... High purity genomic DNA was isolated using the Blood & Cell Culture DNA Mini Kit (Qiagen 13323) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... using the MagAttract 96 cador Pathogen Kit in a BioSprint 96 nucleic acid extractor (Qiagen, Hilden, Germany), according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2022Quote: ... DNA was resuspended in 30 μL of mQ water and incubated with 1 μL of RNase (10 mg/mL prepared from a stock of 100 mg/mL of RNase A from Qiagen, Darmstadt, Germany) at 37 °C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... plasmids were purified from 100 ml of overnight culture using a plasmid maxiprep kit (Qiagen). Before transfection ...
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bioRxiv - Plant Biology 2022Quote: ... Afterwards the Genomic Tip 100 was washed twice with 7.5 mL QC Buffer (Qiagen, Germany) per wash ...
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bioRxiv - Genomics 2022Quote: ... to which 4 μL of RNase A (100 mg/mL) was added (Qiagen, Germantown MD). The cell lysate was applied to a DNeasy Mini spin column ...
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bioRxiv - Microbiology 2022Quote: ... Afterwards the pellet was resuspended in 1 ml of 100 % RNAlater/ RNAprotect Tissue Reagent (Qiagen) and kept on ice ...