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Citations for Qiagen :
1 - 50 of 10000+ citations for Glutathione Colorimetric Detection Kit 200 Low Cuvettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... NSP1 fraction was loaded on Glutathione HiCap resin (Qiagen) equilibrated with the dialysis buffer and proteins were eluted by the same buffer supplemented with 50 mM glutathione ...
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bioRxiv - Developmental Biology 2022Quote: ... Libraries were prepared in-house using the Ultra-low Input Library kit (Qiagen; #180495). Libraries were sequenced with single-end 50 bp reads on the illumina HiSeq 4000 sequencing platform (n=5 samples per experimental group).
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bioRxiv - Cancer Biology 2023Quote: ... The libraries were then prepared using the Qiaseq Ultra Low Input Library Kit (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were immobilized on a glutathione agarose gravity flow column (Qiagen) washed 5 times with PBS solution and eluted in 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Genetics 2020Quote: ... DNA was extracted from the low and high bulks using a Gentra Puregene Kit (Qiagen®). Whole genome sequencing was performed in an Illumina® Hiseq platform (read length = 2 × 125) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and sequential further passage through glutathione-Sepharose 4B and Ni-NTA (QIAGEN) resins ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of targets was performed with miRCURY LNA SYBR® Green PCR Kits (Qiagen) according to the manufacturer’s instructions in a MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... using SYBR-Green based detection system with QuantiNova SYBR green PCR Kit (Qiagen, 208052). Relative expression of mRNA was calculated through ΔΔCt method using the ribosomal protein RP49 RNA expression as internal control for each sample ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of targets was performed with miRCURY LNA SYBR® Green PCR Kits (Qiagen) according to the manufacturer’s instructions in a MyiQ™2 Two-Color Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... roots (200 mg) and rhizosphere (200 mg) were transferred into PowerBead Tubes from the DNeasy PowerSoil kit (QIAGEN) where 1 mL of PBS buffer was added ...
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bioRxiv - Plant Biology 2020Quote: ... and the GST fusion proteins were purified by Glutathione HiCap Matrix columns (Qiagen) and eluted using 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Briefly proteins were purified by glutathione-Sepharose (Cytiva) or Ni-NTA agarose (Qiagen) affinity chromatography ...
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bioRxiv - Cell Biology 2021Quote: ... Fractions were eluted using 10 mM reduced glutathione through a polypropylene column (Qiagen), and those with the highest concentrations combined ...
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bioRxiv - Cancer Biology 2020Quote: ... for candidate mRNA detection by use of the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen), following manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... for candidate mRNA detection by use of the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen), following manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA over 200 nt was first isolated using RNeasy kit (Qiagen). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... for the SARS-CoV-2 low stringency screen with the QIAmp DNA Blood maxi kit (Qiagen ref. 51194). In a first PCR step ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA extraction was performed using 200 μl cCM or 200 μL pooled sEV fractions together with the miRNeasy mini kit (Qiagen). Synthetic oligonucleotides obtained from the miRCURY Spike-In kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA extraction was performed as previously described 50 using 200 μl of plasma or 200 μl of pooled EV fractions and the miRNeasy mini kit (Qiagen). Samples were thawed at room temperature followed by centrifugation at 12,000×g for 5 minutes at 4°C to remove any debris ...
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bioRxiv - Cell Biology 2021Quote: ... coli and purified with glutathione Sepharose beads (GE) or Ni-NTA spin columns (Qiagen), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... coli and purified with glutathione Sepharose beads (GE) or Ni-NTA spin columns (Qiagen), respectively ...
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bioRxiv - Genetics 2021Quote: ... The western blot analysis was performed according to the QIAexpress detection kit and the protocol (Qiagen) with the following modifications ...
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bioRxiv - Molecular Biology 2021Quote: ... The QuantiTect SYBR® Green PCR Kit was used for this biomolecular detection (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2024Quote: ... Amplification and detection were performed using the QIAcuityTM OneStep Advanced Probe Kit (Qiagen, Cat. No. 250132), with primers and probe targeting the influenza A virus M gene segment (specific amplicon length is 110 bp) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was extracted from cells maintained in low-serum conditions (0.5% calf serum) for 22 hours using RNeasy purification kit (Qiagen) and treated with DNase on column ...
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bioRxiv - Molecular Biology 2020Quote: ... were separated in 1.2% (w/v) low melting agarose gels and the ∼0.6 kb band was purified (QIAEX II Gel Extraction kit, Qiagen). This fragment was used as a template in a second PCR reaction with oligonucleotides VHH-Sfi2 (5′-GTC CTC GCA ACT GCG GCC CAG CCGGCC ATG GCT CAG GTG CAG CTG GTG GA-3’ ...
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bioRxiv - Evolutionary Biology 2021Quote: ... with subsequent treatment of the fragments with bisulfite to convert unmethylated cytosines with the low DNA input (1ng-2μg) protocol in the Qiagen EpiTect Fast Bisulfite Conversion kit (Qiagen). Converted DNA was subjected to 12 cycles of PCR amplification to enrich for adapter-ligated fragments with MyTaq Mix (Bioline Inc.) ...
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bioRxiv - Cancer Biology 2023Quote: DNA libraries for chromatin immunoprecipitation sequencing were prepared using QIAseq Ultra Low Input Library Kit (QIAGEN, ZZ-QG-180492) for two independent REST-ChIP experiments ...
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bioRxiv - Biochemistry 2023Quote: ... yielding approximately 200 μg using the Puregene Core Kit A from Qiagen. The extracted DNA was then subjected to digestion using a combination of HinfI and RsaI enzymes ...
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bioRxiv - Molecular Biology 2023Quote: ... the mixture was incubated with Glutathione Sepharose™ 4B (Cytiva) and Ni-NTA Superflow (Qiagen). The Hs53BP1 fragment without its GST tag was recovered in the flow through and concentrated using Vivaspin concentrator (Sartorius).
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bioRxiv - Genetics 2019Quote: ... Ligation products were size-selected (250-300 bp) and purified from a 2% low-melting agarose gel using the MinElute Gel Extraction Kit (Qiagen). Ampure XP beads (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted at low speed (~1000 RPM) and lysed with one mL RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen).
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bioRxiv - Neuroscience 2022Quote: ... The heavy-chain specific amplicon was isolated using electrophoresis with low-melting point agarose extraction with the QIAquick Gel Extraction kit (Qiagen). A secondary amplification was performed using a modification of the primers (VHH-Esp-For ...
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bioRxiv - Microbiology 2023Quote: ... Stocks were passaged once at low multiplicity on MDCK-SIAT1 cells and RNA was extracted using the QIAmp Viral RNA mini kit (Qiagen). Genomic segments encoding the HA and NA proteins were amplified by reverse transcription polymerase (RT-PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... For tissues or isolated hepatocytes with high-fat and low-fat content the total RNA was obtained using a RNeasy Mini Kit (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... mixed and 200 μl were cleaned up using QIAquick PCR Purification kit (Qiagen). For PCR2 ...
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bioRxiv - Biophysics 2023Quote: ... The cell pellet was resuspended in 200 μl P1 buffer (QIAGEN Miniprep Kit), followed by addition of 200 μl P2 buffer for cell lysis ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the total RNA was extracted from the low-passage infected cell culture supernatant (500 µl) using an RNeasy Mini Kit (QIAGEN, Germany), and cDNA synthesis was carried out using an oligo d(T)15 primer and MMLV reverse transcriptase (Promega ...
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bioRxiv - Microbiology 2021Quote: ... The amplified products were analyzed onto 1% agarose gel (low EEO) and extracted and purified from the gel using the QIAprep Miniprep Kit (Qiagen, Netherlands). The 16S rRNA sequencing reactions were performed using 785F (5’-GGATTAGATACCCTGGTA-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... RNA-bead complexes were washed in high and low salt buffer and RNA was eluted from the RNA-bead complexes using the Qiagen RNeasy Plus Micro Kit (Qiagen 74034). Isolated m6A-enriched RNA was immunoprecipitated a second time using 2 µg of a second anti-m6A antibody (Abcam ab151230) ...
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bioRxiv - Molecular Biology 2021Quote: ... isolated embryos were individually collected in 200 μL of RLT buffer (QIAGEN RNeasy Kit). 50 μL were used for extraction of genomic DNA (Invitrogen PureLink genomic DNA mini kit) ...
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bioRxiv - Microbiology 2021Quote: ... and small RNAs (< 200 nt) were discarded using the RNeasy MinElute Cleanup Kit (Qiagen). For the generation of TSS cDNA libraries ...
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bioRxiv - Microbiology 2022Quote: ... 200 mL of the pooled library were purified using the MinElute purification kit (Qiagen) and final elution of the BarSeq library was performed in 30 mL in DNAse and RNAse free water.
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bioRxiv - Physiology 2019Quote: Total RNA (100–200 islets/mouse) was extracted using the RNeasy Mini Kit (Qiagen). RNA quality and quantity was measured on the Agilent 2200 TapeStation System ...
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bioRxiv - Neuroscience 2021Quote: ... RNA (200 ng) was reverse-transcribed using the QuantiTect Reverse Transcription kit (Qiagen 205313). Quantitative PCR reactions were carried out in duplicates with SYBR Green I Master Mix (Roche S-7563 ...
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bioRxiv - Microbiology 2021Quote: ... putida type strain cells (200 μl) was extracted using MagAttract HMW DNA Kit (Qiagen). The DNA was eluted in 100 uL AE buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and passed through 1 mL of glutathione–Sepharose 4B and then onto a Ni-NTA column (QIAGEN) to capture the residual GST- and His-tag fragments ...
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bioRxiv - Cell Biology 2022Quote: ... Condensin I was bound to Glutathione Sepharose 4B beads (Cytiva) in a 5 mL polypropylene column (Qiagen) using GST- tagged mSMC4 and eluted by PreScission Protease (Cytiva ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was incubated under gentle shaking overnight with glutathione matrix (Qiagen; 1ml per 1000ml cell culture) at 4°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Ligated DNA fragments ranging in size from 300 to 450 base pairs (bp) were extracted from 2% low-melt agarose gels and purified using a MinElute gel extraction kit (Qiagen, Valencia, CA). The recovered fragments were amplified using PCR ...