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Citations for Qiagen :
51 - 100 of 259 citations for Glucose Transporter 12 GLUT12 SLC2A12 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... After 12 hours of stimulation cells were pelleted and lysed in RNA lysis buffer (RLT, Qiagen). RNA was extracted using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... 12 or 24 hours post infection and RNA was isolated using RNAeasy RNA extraction kit (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Human cancer stem cells RT2 Profiler PCR arrays were purchased from Qiagen (Cat# PAHS-176ZA-12) and used in combination with a 7900 HT real-time PCR system (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2022Quote: ... with blinding to treatment groups.12 Bilateral biceps muscles were first bead homogenized (TissueLyser II; Qiagen) at a frequency of 30 Hz for 3 cycles of 2 minutes in lysis buffer [10 mM Tris (pH 7.4) ...
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bioRxiv - Genomics 2022Quote: ... The 12 wells from individual plate rows were pooled and cleaned (QIAquick PCR Purification Kit, QIAGEN), and the 30μl elutions resulting from every prep were combined and vortexed thoroughly ...
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bioRxiv - Immunology 2023Quote: ... DNA was isolated on day 12-16 by using the DNeasy Blood and Tissue Kit (Qiagen). The FKBP12 site was amplified with the PlatinumTM SuperFi DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... We purified genomic DNA from the 12 lysogens using the DNeasy Blood and Tissue Kit (Qiagen) and determined the genome sequences of Stx2a phages lysogenized in each lysogen by the long PCR-based strategy ...
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bioRxiv - Systems Biology 2022Quote: ... 12 μl of 1 M Tris-HCl (pH 6.5) and 1 μl RNAse A (Qiagen cat # 19101) were added to the sample and incubated for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... the 15 mm diameter coverslips were placed in 12 well plate with 600μl of Buffer RLT (Qiagen). These plates were stored at −80 °C until RNA extraction ...
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bioRxiv - Genetics 2020Quote: ... cDNA was amplified with 12 cycles of PCR and purified using the QIAquick PCR Purification Kit (Qiagen). The libraries were size-selected on a 6% polyacrylamide gel ...
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bioRxiv - Microbiology 2021Quote: ... Protein lysates were separated on NuPage 5-12% Bis-Tris or 3-8% Tris-Acetate gels (Novex, Qiagen) using MES running buffer (Novex ...
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bioRxiv - Developmental Biology 2022Quote: ... and the number of transcripts per million (TPM) was determined using the CLC Genomics Workbench 12 (Qiagen, USA). Results were mined for expression of factors implicated in SARS-CoV-2 infection ...
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bioRxiv - Genetics 2019Quote: ... PCR products corresponding to the 12 mutated codons were pooled and purified with a PCR purification kit (Qiagen). The PCR product was phosphorylated with T4 Polynucleotide Kinase ...
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bioRxiv - Molecular Biology 2023Quote: 12-30 reactions were pooled and purified by PCR column purification using QIAquick PCR purification kit (Qiagen, 28104). DNA concentration was measured by Nanodrop 1000 (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: All datasets were combined (∼250 million reads) assembled de novo with both CLC genomics workbench 12 (Qiagen, 12.0) and Trinity using default settings ...
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bioRxiv - Biochemistry 2023Quote: ... genomic DNA was extracted from 1x107 parasites 12 days after transfection using the DNeasy Blood & Tissue Kit (Qiagen) with non-transfected Cas9 positive and wild type L ...
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bioRxiv - Microbiology 2022Quote: ... α-His antibody (Qiagen) was used at 1:5,000 dilution to detect the presence of rRH5 in Native-PAGE.
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bioRxiv - Genomics 2020Quote: ... The total RNA solution from 12 wells are passed through one column of RNeasy mini kit (cat.74104, QIAGEN) to obtain approximately 100 g of purified total RNA ...
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bioRxiv - Cancer Biology 2019Quote: ... and 12-273 BM cells at 80% confluence in 6 well plates using the RNeasy Mini Kit (Qiagen 74104). 600 ng of RNA was subjected to DNase I treatment and reverse transcription ...
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bioRxiv - Genomics 2019Quote: Multiple sequence alignments were generated using a progressive alignment algorithm within the CLC Genomics Workbench (Version 12, Qiagen Ltd) [94] ...
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bioRxiv - Bioengineering 2021Quote: ... The complex (12 mg/ml) was screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were plated in 12 well plates at a dilution of 1.5×106 and were transfected with the indicated constructs using Effectene (Qiagen) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: 2.0 × 105 HeLa cells were transfected in 12 well plates with control (NTC: all-stars non-targeting control, Qiagen) or DDX39B-targeting (D11 ...
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bioRxiv - Biochemistry 2020Quote: ... 12 000 rpm 45 min) and the supernatant was loaded onto a column containing NiNTA resin (1 mL, Qiagen) pre-equilibrated with buffer A (10 mL) ...
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bioRxiv - Genomics 2022Quote: ... 10 budgerigar samples and 12 zebra finch samples) using the DNeasy® Blood & Tissue Kit (Cat. No. 69581, Qiagen). For PCR ...
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bioRxiv - Cell Biology 2022Quote: siRNA targeting ARRB2 (ARRB2-12, Cat #SI03099103) and a non-targeting control (AllStars, Cat # 1027415) was purchased from Qiagen. A pool of 4 siRNAs targeting human RME-8/DNAJC13 was purchased from Dharmacon/Horizon Discovery (Cat #L-010651-01 ...
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bioRxiv - Microbiology 2022Quote: ... Further sequence analysis of 250-bp paired-end read data was run with CLC Genomics Workbench 12 (Qiagen Bioinformatics). The obtained reads were trimmed with bla and 16S rRNA primer sequences bla_293F ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA precipitation was performed for at least 12 hours and MinElute Spin Columns were used for elution (Qiagen, Germany). Historic samples were processed in a dedicated facility for highly degraded specimens.
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bioRxiv - Immunology 2023Quote: ... The protein complexes were adjusted to 8.6–12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
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bioRxiv - Cancer Biology 2023Quote: ... mixed with 100 μl of serum-free medium and 12 μl of HiPerfect Transfection Reagent (Qiagen, Germantown, MD, USA). Effectiveness of ARSB silencing was confirmed by activity assay ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with primary antibody (Penta·His Antibody, QIAGEN®, 1:2000 dilution) in 1% bovine serum albumin in PBST for 2h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Cells with lysis buffer were pulverized in a 10 mL jar containing a 12 mm grinding ball using a TissueLyser II (QIAGEN) by performing 5 rounds of 3 min at 15 Hz ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA of CDC-EVs (n=12) and MSC-EVs (n=4) was extracted using the miRNeasy Serum/Plasma kit (QIAGEN). Library construction was performed according to the manufacturer’s protocol using the TruSeq small RNA Library Kit (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... Each complex was adjusted to 12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Genomics 2020Quote: ... at which point we combined the resulting solution with 12 mL of PB buffer and followed standard MinElute PCR Purification Kit (Qiagen) protocol ...
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bioRxiv - Genomics 2020Quote: ... total RNA was extracted from cells grown in five different salinity treatments (0, 2, 12, 24, 36 parts per thousand) using the RNeasy Plant Kit (Qiagen), and 15 Illumina libraries were prepared using the Kapa mRNA HyperPrep kit and sequenced on the Illumina HiSeq2000 platform at the Beijing Genomics Institute.
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bioRxiv - Genomics 2019Quote: ... FSM sub-groups and 12 rats in the CS sub-group) using the RNeasy Plus Mini Kit (Qiagen, Hilden Germany). RNA extraction ...
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bioRxiv - Immunology 2019Quote: For every qPCR assay a total of 3 biological samples (12 larvae each) were collected in QIAzol lysis reagent (Qiagen) and RNA was extracted using the miRNeasy mini kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized using the miScript II RT Kit with Hiflex buffer from 12 μL of extracted total RNA according to product manual (Qiagen). Expression of mRNA was quantified using QuantiTect SYBR Green PCR kit (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... from explants that had been cultured on RIM for 12 hours in the semi-synchronous LR induction system and purified using the RNeasy microkit (QIAGEN). Affymetrix ATH1 microarrays were hybridized with biotinylated cRNA targets prepared from the RNA samples according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The complete dataset of 455,774 sequences with coverage across the genome were aligned in CLCbio Genomics Workbench 12 (QIAGEN Bioinformatics) to the GenBank reference sequence NC_045512.2 ...
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bioRxiv - Systems Biology 2020Quote: ... We incubated these cultures on a shaker at 225 rpm and 32°C for 12-14 hours then pelleted the cultures by centrifugation and isolated plasmid using the EndoFree plasmid maxiprep kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... total RNAs were extracted from 12-day-old seedlings grown during long days using a RNeasy Plant Mini kit (Qiagen). Following RNA purification ...
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bioRxiv - Microbiology 2020Quote: ... then entered an extraction pipeline in which 12 samples were processed in parallel on the QIAvac 24 Plus Manifold (Qiagen).
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bioRxiv - Cell Biology 2021Quote: ... The input and immunoprecipiated DNA were reverse cross-linked at 60 degrees for 12 hours and then purified using a QIAquick PCR Purification kit (QIAGEN). The input DNA was diluted 1:100 ...
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bioRxiv - Genetics 2022Quote: ... and larvae (12 CRISPR-treated, 20 water-injected and 13 uninjected) selected for sequencing by using DNeasy Blood & Tissue Kit (QIAGEN) Purity and concentration of DNA samples were measured with a NanoDrop spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: DNA was isolated from PBMC and lymph nodes cells collected 12 weeks post-challenge using the QIAamp micro kit (Qiagen) following the manufacturer’s instructions and eluted in 50 μL of water ...
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bioRxiv - Microbiology 2021Quote: ... The protein complex was adjusted to 12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
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bioRxiv - Cell Biology 2021Quote: ... 10∼12 ml cell culture medium was subjected to extracellular RNA isolation using the exoRNeasy Maxi kit (Qiagen, Cat# 77164) with minor modifications ...
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bioRxiv - Microbiology 2019Quote: U1 cells were treated with 100 μg/ml concentration of exosomes for 12 h and total RNA was isolated using RNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...