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Citations for Qiagen :
201 - 250 of 659 citations for Ferritin light chain FTL Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Recombinant-construct with N-terminal His-tag was purified through IMAC-based Ni-NTA (Qiagen). IPTG induced ...
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bioRxiv - Microbiology 2020Quote: ... were coated with 100 ng/well of mouse anti-Penta His BSA-free antibody (Qiagen) in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... a 1:2000 dilution of a mouse anti-Penta-His Alexa Fluor 647 conjugate (Qiagen) was used.
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bioRxiv - Biochemistry 2021Quote: ... Monoclonal mouse anti-StrepII and anti-His antibodies were obtained from QIAGEN (catalog number 34850) and Genscript (catalog number A00186) ...
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bioRxiv - Microbiology 2022Quote: ... BapR-CPD-His was affinity purified from cleared lysates using Ni-NTA agarose beads (Qiagen) with gentle rocking at 4°C for 2 hours ...
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bioRxiv - Biochemistry 2021Quote: ... containing an N-terminal His-tag was removed on a Ni-NTA agarose column (Qiagen). Proteins were additionally purified through a Superdex 200 26/60 size exclusion column (Pharmacia Biotech ...
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bioRxiv - Molecular Biology 2021Quote: ... MCP (His-HaloTag-2xMCP) was purified using its histidine tag with Ni-NTA Agarose (Qiagen) following the manufacturer’s instructions with minor modifications ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CSF3R protein was enriched by nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen 30210) and further purified by size-exclusion chromatography on a Superdex 75 10/300 GL column (Cytiva 17517401 ...
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bioRxiv - Cell Biology 2022Quote: ... Binding of Ad2 was detected by an HRP-conjugated monoclonal antibody to His-tag (Qiagen) expressed by the fiber knob ...
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bioRxiv - Immunology 2022Quote: ... after which the cells were stained with anti-Penta-His-AF647 (1 µg/mL; Qiagen) for 30 minutes at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... the precipitates were subjected to Western blot analysis with anti-penta-His (1:2000, Qiagen) and anti-FLAG M2 (1:4000 ...
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bioRxiv - Microbiology 2023Quote: ... and were probed with either horseradish peroxidase (HRP)- conjugated primary antibodies against His-tag (Qiagen) or with rabbit primary antibodies against HA-tag (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The His-tagged proteins were purified using the QIAexpress Ni-NTA Protein Purification System (Qiagen) with 0.1 M ...
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bioRxiv - Microbiology 2024Quote: ... His-tag-affinity chromatography-purifcation was performed with a Ni-NTA agarose (Qiagen, Hilden, Germany) gravity flow column ...
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bioRxiv - Cell Biology 2020Quote: Human DUOX1: GPH1004464(-)02A (Qiagen)
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bioRxiv - Cell Biology 2020Quote: Human DUOX2: GPH1018346(-)08A (Qiagen)
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bioRxiv - Cell Biology 2020Quote: ... Human RPLP0 (primers from QIAGEN) was used as reference gene for cDNA from Caco-2 samples ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715 ...
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bioRxiv - Cancer Biology 2023Quote: ... with Quantitect human primers (Qiagen) for genes ID1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out using a QuantiFast SYBR Green PCR kit (QIAGEN, 204056) on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Genetics 2021Quote: Relative telomere length of MDS patients was measured by quantitative polymerase chain reaction (qPCR) as previously reported.8 K562 cell DNA was extracted using the QIAamp Blood Mini kit (Qiagen). Telomere length was measured by two orthogonal methods ...
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bioRxiv - Microbiology 2020Quote: ... One-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) following the standard protocol for a QuantStudio 7 Flex RT-PCR system ...
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bioRxiv - Biochemistry 2020Quote: ... low melting point agarose gel and the approximately 700 base pair band corresponding to the heavy chain only immunoglobulin was extracted and purified using QIAquick Gel Extraction Kit (Qiagen). A secondary amplification of the VHH domain was performed using modified primers (VHH-Esp-For ...
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bioRxiv - Microbiology 2020Quote: ... Gene expression was assessed using SYBR GreenER based-quantitative polymerase chain reaction (qPCR) or RT2 profiler arrays (Qiagen Ltd, UK). For SYBR GreenER based-qPCR analyses ...
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bioRxiv - Cell Biology 2022Quote: ... Drosophila S2 cells were transfected with mNeonGreen-tagged kinesin-1 full-length heavy chain (KHC) and Halo-tagged PLP584-1811 (Effectene, QIAGEN). The lysates were harvested for TIRF motility assays at ∼48 h post-transfection ...
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bioRxiv - Microbiology 2020Quote: Quantitative polymerase chain reaction (qPCR) analyses were performed on the new DNA extracts using PowerSoil DNA Isolation Kit (12888-50, QIAGEN) to estimate the abundance of bacteria and archaea at different depths in the sediment core ...
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bioRxiv - Molecular Biology 2022Quote: ... This 1st round IgK PCR reaction was used for the 2nd round PCR of IgK chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The same cDNA reaction was also used for the 1st round PCR of IgK chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... This 1st round IgG PCR reaction was used for the 2nd round PCR of IgG chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... This cDNA reaction was used for the 1st round PCR of IgG chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
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bioRxiv - Bioengineering 2021Quote: ... One-step reverse transcription quantitative polymerase chain reaction (RT-qPCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) with 7.8 μl purified viral RNA or specified quantities (2.5 ng or 100 ng ...
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bioRxiv - Neuroscience 2022Quote: ... The heavy-chain specific amplicon was isolated using electrophoresis with low-melting point agarose extraction with the QIAquick Gel Extraction kit (Qiagen). A secondary amplification was performed using a modification of the primers (VHH-Esp-For ...
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bioRxiv - Bioengineering 2023Quote: Reverse transcription of isolated miRNAs for quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed with a miScript II RT Kit (Qiagen) for selected miRNA targets ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Light organs were then transferred into 180 ul of pre-heated (98°C) ATL buffer (QIAGEN) and incubated at 98°C for 15 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR amplification was performed on a Roche light cycler using OneStep qPCR SYBR green kits (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... Bands were visualized with UV light and excised followed by DNA isolation (Qiagen Gel Extraction kit). Extracted DNA was analyzed by Sanger sequencing.
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bioRxiv - Microbiology 2020Quote: ... BipA-His was purified from the soluble fraction by affinity chromatography using Ni-NTA agarose (QIAGEN) previously equilibrated with equilibration buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 6x-His-Smt3 was purified from BL21 E.coli cells using a Ni-NTA affinity column (Qiagen) following manufacturer indications ...
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bioRxiv - Cancer Biology 2021Quote: ... Signal was detected using mouse anti-His antibody from Qiagen (Cat No./ID: 34660 1:500). Anti-mouse HRP was used for colorimetric development (Cat No./ID-A8924 1:1000) ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged BASP1 derivatives were prepared using Ni-NTA beads following the manufacturers instructions (Qiagen). Purified proteins were dialysed into Buffer D and stored at −80°C.
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bioRxiv - Plant Biology 2021Quote: ... HIS-tagged proteins were purified by metal-affinity chromatography using Ni-NTA resins (Qiagen, Hilden, Germany). Protein concentration was estimated by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and His-tagged Fabs were isolated from cell supernatants using Ni-NTA columns (Qiagen, Hilden, Germany). IgGs ...
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bioRxiv - Cancer Biology 2019Quote: ... 50 nM siRNA/well were used for transfection using 0.8 µl/well of Hi-Perfect (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The expressed protein was purified as His-tagged fusion protein using Ni-NTA agarose beads (Qiagen) and analyzed by SDS-PAGE.
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bioRxiv - Neuroscience 2020Quote: ... The His-tagged target protein was purified by Ni-NTA gravity-flow chromatography (Qiagen, Hilden, Germany). The eluted protein was subjected to MonoQ 10/100 GL (GE Healthcare Lifesciences ...
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bioRxiv - Biophysics 2023Quote: ... the glass was additionally treated with biotinylated anti-hexahistidine monoclonal antibody (Penta-His Biotin Conjugate; Qiagen) as in Duchi et al.23 and Dulin et al.31.
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bioRxiv - Biochemistry 2024Quote: ... Proteins modified with 6×-His-Ub was purified using Ni-NTA Sepharose (142350243, Qiagen, Alameda, CA) on a rotating platform for 2 hours at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified using the His-tag Protein Purification Kit (Ni-NTA Agarose, QIAGEN GmbH), GST-tag Protein Purification Kit (Glutathione Sepharose ...
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bioRxiv - Bioengineering 2019Quote: Genes were quantified by one-step quantitative reverse transcription-polymerase chain reaction (q-PCR) using the QuantiFast SYBR Green RT-PCR kit (Qiagen, UK). A master mix was prepared by mixing 6.25 μl SYBR green ...
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Global genetic patterns reveal host tropism versus cross-taxon transmission of bat BetacoronavirusesbioRxiv - Evolutionary Biology 2020Quote: ... All RNA extracts were subjected to reverse transcription polymerase chain reaction (RT-PCR) using the one-step RT-PCR kit (Qiagen, USA) and PanCoV F2 (5’-AAR TTY TAY GGH GGY TGG-3’ ...