Labshake search
Citations for Qiagen :
1 - 50 of 331 citations for Ferritin heavy chain FTH1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... plasmids bearing chimeric antibody heavy and light chain genes were prepared (EndoFree Plasmid Mega prep Kit, QIAGEN) and transfected into Human Embryonic Kidney cells (HEK293F ...
-
bioRxiv - Biochemistry 2020Quote: ... low melting point agarose gel and the approximately 700 base pair band corresponding to the heavy chain only immunoglobulin was extracted and purified using QIAquick Gel Extraction Kit (Qiagen). A secondary amplification of the VHH domain was performed using modified primers (VHH-Esp-For ...
-
bioRxiv - Cell Biology 2022Quote: ... Drosophila S2 cells were transfected with mNeonGreen-tagged kinesin-1 full-length heavy chain (KHC) and Halo-tagged PLP584-1811 (Effectene, QIAGEN). The lysates were harvested for TIRF motility assays at ∼48 h post-transfection ...
-
bioRxiv - Immunology 2020Quote: ... cDNA products were then used in nested PCR for heavy/light chain variable region amplification with HotStarTaq Plus DNA Polymerase (QIAGEN # 203643) and specific primer sets described previously 34,35 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Approximately 700 base pair bands corresponding to the heavy-chain only immunoglobulin were extracted (QIAquick Gel Extraction Kit: Qiagen K.K., Tokyo, Japan). Nested PCR was performed to amplify coding genes of VHH domains using the VHH-PstI-For and VHH-BstEII-Rev primers and subcloned into the pMES4 phagemid vector5 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and lower bands corresponding to the heavy-chain only immunoglobulin were extracted using the QIAquick Gel Extraction Kit (Qiagen K.K., Tokyo, Japan). Subsequent nested PCR was performed to amplify coding genes of VHH domains using the VHH-PstI-For and VHH-BstEII-Rev primers and subcloned into the pMES4 phagemid vector36 ...
-
bioRxiv - Immunology 2020Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out by using the ‘Human Innate and Adaptive immune Response’ kit (Qiagen, Hilden, Germany) to assess the expression of genes/mRNA ...
-
bioRxiv - Microbiology 2023Quote: ... Polymerase chain reaction (PCR) was performed with the HotStarTaq Plus polymerase (Qiagen) with 5 min initial denaturation at 94°C ...
-
bioRxiv - Microbiology 2020Quote: Polymerase chain reaction (PCR) was performed using Taq DNA Polymerase (QIAGEN, Hilden, Germany). The fungal ITS-28S rRNA gene region was obtained using the ITS5 (White et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... Purification of His6-light chain TeTx was done using Ni-NTA resin (Qiagen) in 50 mM Tris HCl pH 7.4 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... QuantiTect Probe RT-PCR (reverse transcription polymerase chain reaction) Kit (QIAGEN, Hilden, Germany) was used to perform RRT-PCR reactions in the ABI 7300 Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Evolutionary Biology 2023Quote: Polymerase Chain Reactions (PCRs) were performed using Type-it Microsatellite PCR Kit (Qiagen) in 10 µl reactions ...
-
bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339, human METTL3: QIAGEN SI05020414 and human DDX6 ...
-
bioRxiv - Physiology 2023Quote: ... and real-time polymerase chain reaction (PCR) was performed with Fast SYBR Green (QIAGEN). Gapdh was used as the housekeeping gene ...
-
bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339 ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were grown to approximately 80% confluency in light and heavy media and transfected using Attractene (#301007, Qiagen, Germany) with SBP-empty vector (control cells ...
-
bioRxiv - Immunology 2019Quote: ... Input material (total cytoplasmic mRNA) and efficiently translated mRNA (heavy polysome-associated, >3 ribosomes) were extracted with QIAzol (Qiagen) and purified using RNeasy MinElute Cleanup Kit (Qiagen) ...
-
bioRxiv - Physiology 2020Quote: ... The polymerase chain reaction (PCR) were carried out using Rotor Gene Q (Qiagen, Hilden, Germany) with initial incubation at 95 °C for 15 min ...
-
bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction was conducted using miRCURY LNA miRNA SYBR PCR kit (Qiagen #339345) as per manufacturer’s instruction on BioRad CFX96™ System in 96-well plates in 3-6 repeats ...
-
bioRxiv - Molecular Biology 2021Quote: Polymerase chain reaction (PCR) products were gel purified using Qiagen QlAquick gel purification columns (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757 ...
-
bioRxiv - Neuroscience 2020Quote: ... Quantitative real-time polymerase chain reactions were performed using the QuantiFast SYBR Green PCR Kit (QIAGEN) and the CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Neuroscience 2022Quote: ... Transgenic animals were identified by polymerase chain reaction of tail DNA (DNeasy kit, Qiagen, Hilden, Germany) using primers for Cre (Schönig et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... in a Rotor-Gene® Q real-time polymerase chain reaction machine (Qiagen Inc., Germantown, MD). The PCR primers were designed with Primer Premier 6 (Premier Biosoft Int ...
-
bioRxiv - Neuroscience 2020Quote: ... Extracted DNA was used for genotyping by polymerase chain reaction (PCR) (Taq DNA polymerase, Qiagen, Hilden, Germay), using primers flanking the endogenous CAG repeat in exon 10 of the murine Atxn3 gene (primer sequences ...
-
bioRxiv - Cancer Biology 2019Quote: The cDNA of wild-type ecATCase and regulatory chain of ecATCase-holo were amplified by PCR (Qiagen Kit) using BL21(DE3 ...
-
bioRxiv - Genetics 2022Quote: ... human SETD2 siRNA (Qiagen # 1027416 ...
-
bioRxiv - Genetics 2022Quote: ... human CRY2 siRNA (Qiagen # 1027416 ...
-
bioRxiv - Genetics 2022Quote: ... human CPSF6 siRNA (Qiagen # 1027416 ...
-
bioRxiv - Immunology 2021Quote: ... was then added to a polymerase chain reaction (PCR) mix containing 12.5 μl of 2× Multiplex PCR mix (Qiagen), 9 μl of H2O ...
-
bioRxiv - Microbiology 2020Quote: ... Insertion of the dcas9/lacI cassette at the desired chromosomal location in strain CJW_Bb362 was confirmed by polymerase-chain reaction (PCR) analysis of isolated total genomic DNA (DNeasy Blood & Tissue Kit, Qiagen), using the following primer pairs ...
-
bioRxiv - Genetics 2020Quote: ... Multiplex polymerase chain reaction (PCR) was performed using three primer pairs and the Multiplex PCR Kit (QIAGEN, Hilden, Germany). Each reaction contained 2× QIAGEN multiplex PCR master mix ...
-
bioRxiv - Microbiology 2023Quote: ... DNA fragments that were generated by the polymerase chain reaction (PCR) were purified using the PCR Purification Kit (Qiagen), Monarch® PCR & DNA Cleanup Kit (NEB) ...
-
bioRxiv - Cell Biology 2020Quote: Human DUOX1: GPH1004464(-)02A (Qiagen)
-
bioRxiv - Cell Biology 2020Quote: Human DUOX2: GPH1018346(-)08A (Qiagen)
-
bioRxiv - Cell Biology 2020Quote: ... Human RPLP0 (primers from QIAGEN) was used as reference gene for cDNA from Caco-2 samples ...
-
bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715 ...
-
bioRxiv - Cancer Biology 2023Quote: ... with Quantitect human primers (Qiagen) for genes ID1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out using a QuantiFast SYBR Green PCR kit (QIAGEN, 204056) on a StepOnePlus real-time PCR system (Applied Biosystems) ...
-
bioRxiv - Genetics 2021Quote: Relative telomere length of MDS patients was measured by quantitative polymerase chain reaction (qPCR) as previously reported.8 K562 cell DNA was extracted using the QIAamp Blood Mini kit (Qiagen). Telomere length was measured by two orthogonal methods ...
-
bioRxiv - Microbiology 2020Quote: ... One-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) following the standard protocol for a QuantStudio 7 Flex RT-PCR system ...
-
bioRxiv - Microbiology 2020Quote: ... Gene expression was assessed using SYBR GreenER based-quantitative polymerase chain reaction (qPCR) or RT2 profiler arrays (Qiagen Ltd, UK). For SYBR GreenER based-qPCR analyses ...
-
bioRxiv - Microbiology 2020Quote: Quantitative polymerase chain reaction (qPCR) analyses were performed on the new DNA extracts using PowerSoil DNA Isolation Kit (12888-50, QIAGEN) to estimate the abundance of bacteria and archaea at different depths in the sediment core ...
-
bioRxiv - Molecular Biology 2022Quote: ... This 1st round IgK PCR reaction was used for the 2nd round PCR of IgK chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... The same cDNA reaction was also used for the 1st round PCR of IgK chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... This 1st round IgG PCR reaction was used for the 2nd round PCR of IgG chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... This cDNA reaction was used for the 1st round PCR of IgG chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
-
bioRxiv - Bioengineering 2021Quote: ... One-step reverse transcription quantitative polymerase chain reaction (RT-qPCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) with 7.8 μl purified viral RNA or specified quantities (2.5 ng or 100 ng ...
-
bioRxiv - Neuroscience 2022Quote: ... The heavy-chain specific amplicon was isolated using electrophoresis with low-melting point agarose extraction with the QIAquick Gel Extraction kit (Qiagen). A secondary amplification was performed using a modification of the primers (VHH-Esp-For ...
-
bioRxiv - Bioengineering 2023Quote: Reverse transcription of isolated miRNAs for quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed with a miScript II RT Kit (Qiagen) for selected miRNA targets ...