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Citations for Qiagen :
101 - 150 of 709 citations for FcgR4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... were functionalized with biotinylated α-penta-His antibody (Qiagen, Hilden Germany or R&D Systems ...
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bioRxiv - Immunology 2020Quote: Total RNA of IFN-α2 treated HEK293 cells was purified using RNeasy columns (Qiagen) with on-column DNase I digestion ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were transfected with hTRPM3α2-GFP or its mutants using the Effectene reagent (Qiagen). Cells were loaded with 1 μM fura-2 AM (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... His-tagged proteins were purified over Ni-NTA-agarose (Qiagen) with 3 wash steps at 30 mM imidazole and elution with 250 mM imidazole in denaturing buffer ...
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bioRxiv - Microbiology 2019Quote: ... A 10 nM solution of biotinylated penta-His antibody (Qiagen) was incubated for 10 min on the neutravidin-coated surface and excess unbound antibody removed (22 ...
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bioRxiv - Bioengineering 2020Quote: ... Anti-Penta-His Alexa Fluor 488 conjugate was from Qiagen.
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bioRxiv - Microbiology 2021Quote: ... His-tagged proteins were purified with Ni2+-NTA beads (QIAGEN) and were eluted with 300 mM imidazole in PBS buffer ...
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bioRxiv - Microbiology 2022Quote: ... and His-PutA was purified with Ni-column chromatography (Qiagen). The purified His-PutA protein (50 μg ...
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bioRxiv - Biochemistry 2021Quote: Penta-His HRP conjugate was purchased from QIAGEN (Hilden, Germany). Anti-BepA (Narita et al. ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Penta-His-biotin conjugate 1:5000 (Qiagen) overnight ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were purified with Ni2+-NTA beads (Qiagen) and eluted with 300 mM imidazole in PBS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged proteins were bound by Ni-NTA agarose (Qiagen) and eluted with 500 mM imidazole in HSB ...
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bioRxiv - Molecular Biology 2024Quote: ... His-tagged protein was bound to Ni-NTA beads (Qiagen). The beads were washed with 100 mL lysis buffer and protein was eluted with 40 mL elution buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5 µg/mL anti-Penta-His-Alexa Fluor 488 (Qiagen), which recognized the His-tag on PXDN-con4 ...
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bioRxiv - Immunology 2020Quote: ... immunoplates were coated with 0.125ug of Tetra-His antibody (34670; QIAGEN) followed by 2 ug/ml and 5 ug/ml of soluble RBD and NP ...
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bioRxiv - Cancer Biology 2020Quote: ... The bacterial His-tagged proteins were purified under denaturing conditions (Qiagen) and refolded by dialysis as described previously (22) ...
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bioRxiv - Neuroscience 2020Quote: ... cultured and then isolated using a Hi-speed Midiprep kit (Qiagen). Expression was first examined by transfection of N2A cells in vitro ...
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bioRxiv - Cell Biology 2021Quote: ... and His6- Vps4 was detected using Penta-His Antibody (Qiagen, Germany) per manufacturer instruction ...
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bioRxiv - Microbiology 2022Quote: ... Antibodies against His-tag and S-tag were purchased from Qiagen and GenScript respectively ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 647-conjugated Penta-His mAb was purchased from Qiagen, Germantown ...
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bioRxiv - Microbiology 2019Quote: His Tag-Alexa 647 (5 ug/mL) (Qiagen, catalog no. 35370)
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bioRxiv - Cell Biology 2019Quote: ... The His-tagged protein was purified using Ni-NTA resin (Qiagen) following the manufacturer’s instructions and was used for rabbit immunization ...
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bioRxiv - Neuroscience 2020Quote: ... His-tagged TEV protease was removed using Ni-NTA agarose (Qiagen). For FRET experiments ...
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bioRxiv - Immunology 2020Quote: ... His-tagged Fabs were loaded onto Ni-NTA superflow resin (Qiagen) preequilibrated with Buffer A (10 mM Tris ...
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bioRxiv - Biophysics 2022Quote: The following anti-His fluorophore-conjugated antibodies were purchased from Qiagen and used in UV-Bind assays for His-tagged proteins ...
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bioRxiv - Molecular Biology 2022Quote: The His-tagged protein was purified by Ni-NTA agarose (Qiagen). Eluted proteins from beads were then applied to the NGC chromatography system (Bio–Rad) ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was treated with Penta-His MAb (Qiagen, Germantown, MD) followed by HRP-conjugated goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Bound PEX5L was detected immunologically using monoclonal Anti-His Antibodies (Qiagen).
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bioRxiv - Biochemistry 2022Quote: ... His-tagged Usp2-cc was bound to Ni-NTA Agarose (QIAGEN) equilibrated in buffer F (20 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... by retrotranscription of RNAs from HeLa and HEK293-T cells using the QuantiTect Reverse Transcription kit (Qiagen) followed by PCR-mediated amplification and plasmid insertion with the in-Fusion cloning kit (Clontech) ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted from the parental and knockout HEK293 cells using QIAamp DNA mini kit (QIAGEN) and PCR amplified with primers located ~500-600 bp from the sgRNA target site ...
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bioRxiv - Genetics 2023Quote: The extraction of total RNA from HEK293 stable cell lines was isolated by RNeasy mini kit (QIAGEN), and cDNA was reversed with Maxima First Strand cDNA Synthesis Kit (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... Protein was purified through affinity chromatography (Ni-NTA His-Bind resin, Qiagen), followed by gel filtration chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Cell Biology 2022Quote: ... His- tagged human UHRF1 was purified using Ni-NTA sepharose resin (Qiagen). Recombinant E1 (His-UBE1) ...
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bioRxiv - Biochemistry 2021Quote: ... Electroblotted membranes were incubated with penta-His antibody (Qiagen, Valencia, CA, USA). The antigen–antibody complex was visualized with alkaline phosphatase-linked to anti-rabbit IgG followed by staining with 5-bromo-4-chloroindol-2-yl phosphate and nitro blue tetrazolium [36].
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bioRxiv - Biophysics 2020Quote: ... His-tagged Chd1 was purified by Ni-NTA agarose affinity chromatography (Qiagen) and treated with homemade TEV protease to remove the tag ...
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bioRxiv - Biophysics 2020Quote: ... the surface was incubated with 1 nM biotinylated penta-His antibody (Qiagen) in buffer A for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the His-SUMO conjugates were enriched using nickel-nitrilotriacetic acid beads (Qiagen). Upon multiple washing steps ...
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bioRxiv - Biochemistry 2021Quote: ... The primary and secondary antibodies used were anti-penta His (Qiagen #34660) and goat anti-mouse IgG (LiCOR #926-68070 ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged uSpike was purified using Ni-NTA agarose (Qiagen, Germantown, MD) in a gravity flow column ...
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bioRxiv - Microbiology 2019Quote: ... His-cyAbrB1 was purified with the Ni-NTA Fast Start kit (Qiagen). The elution fractions containing the purified protein were loaded onto a PD MidiTrap G-25 column (GE healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... His-NaERF2-like were expressed and purified with Ni-NTA agarose (QIAGEN). Biotin labeled probe EM13 (5’-tagattATCTaattctact-3’ ...
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CHD7 interacts with the nucleosome acidic patch for its efficient activity via its N-terminal regionbioRxiv - Biochemistry 2020Quote: ... His-tagged proteins were purified by Ni-NTA agarose affinity chromatography (Qiagen) and treated with TEV protease to remove the tag ...
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bioRxiv - Neuroscience 2020Quote: ... His-tagged TEV protease was removed by incubation with Ni-NTA (Qiagen) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged protein purification was done with Ni-NTA agarose resin (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged HTa was purified with Ni-NTA agarose beads (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... His-tagged proteins were purified using a nickel column purification step (QIAGEN). Following IMAC ...
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bioRxiv - Plant Biology 2021Quote: ... The MBP/His-tagged proteins were purified using Ni2+-NTA agarose (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: His-tagged MCP/scFv-GFP was purified with Ni-NTA-agarose (Qiagen) following the manufacturer’s instructions with minor modifications ...
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bioRxiv - Cell Biology 2020Quote: ... HIS-TRIM39 proteins were purified on Ni-NTA agarose beads (Qiagen, #1018244) and then eluted in a buffer containing 0.5 M imidazole before dialysis in PBS.