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Citations for Qiagen :
1 - 50 of 1378 citations for Fc Receptor Like Protein 3 FCRL3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Restriction-enzyme-digested WT FCRL3 plasmid lacking the first three Ig-like domains was resolved by gel electrophoresis and subsequent purification (Qiagen Gel Purification Kit). The restriction-enzyme-digested gene blocks were also purified (Qiagen PCR Purification Kit) ...
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bioRxiv - Immunology 2020Quote: ... cDNA was added to the Human Toll-like receptor signaling pathway RT2 Profiler PCR array (Qiagen) and run on an iCycler MyiQ (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... and the cDNA was loaded onto the “Mouse Toll-Like Receptor Signaling Pathway” RT2 Profiler PCR Array according to the manufacturer instructions (Qiagen, Valencia, CA). Expression of the target genes were normalized to the geometric mean of Ct values of the two housekeeping genes Gusb and Hsp90a using the ddCt method (n = 3) ...
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bioRxiv - Biophysics 2021Quote: ... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
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bioRxiv - Immunology 2023Quote: ... supernatants were harvested and His-tagged tIgM-Fc (tFcµ) protein was purified using Ni-NTA Agarose (Qiagen) and subsequently ...
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bioRxiv - Cell Biology 2023Quote: Tissue samples and 3D tendon-like constructs were immersed in RNAlater (Qiagen, Hilden, Germany) and stored at -20°C until analysis ...
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bioRxiv - Physiology 2024Quote: Total RNA from M2-like macrophages was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany) and QIAshredder spin columns (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... Digested protein was mixed with 3 ml of NTA super-flow resin (Qiagen) that had been pre-equilibrated in wash buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The RT² Profiler™ Rat Inflammatory Cytokines & Receptors PCR Array (Qiagen) was used to measure inflammatory cytokines and chemokines in PFC and NAc tissue of rats that underwent heroin SA ...
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bioRxiv - Molecular Biology 2024Quote: ... Recombinant protein was captured by overnight incubation with 3 ml Ni-NTA resin (Qiagen) followed by washing with 30 ml denaturing buffer (8 M urea ...
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of RGS(His)6-tagged 14-3-3 was performed by applying the anti-RGS(His)6 antibody (Qiagen) in combination with a secondary anti-mouse HRP antibody.
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bioRxiv - Immunology 2020Quote: A RT2 profiler array for mouse inflammatory cytokines and receptors (Qiagen, USA) was used according to manufacturer recommendations to measure the expression of 84 inflammatory genes (Table S1) ...
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bioRxiv - Neuroscience 2024Quote: Specific enrichment of nuclear proteins from astrocytes and neurons (NS, SD, 3 biological replicates) was performed using a Qproteome Nuclear Protein Kit (QIAGEN, Cat # 37582) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... residual CD34+ stem-like cells were sorted by FACS and genomic DNA was extracted with the DNeasy kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: The ribosomal DNA transcription assay was performed like described before.[44] Total RNA was extracted using the RNeasy kit from QIAGEN. cDNA was generated using Thermo Fisher Scientific SuperScript® III Reverse Transcriptase and 2 μg RNA following the vendor’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Lysogens were isolated from SPβ-like plaques (Fig 4C) and genomic DNA was prepared using the DNeasy Tissue and Blood Kit (Qiagen) and sequenced via Nanopore sequencing on an R9 PromethION flowcell ...
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bioRxiv - Immunology 2022Quote: ... For RT2 Profiler PCR Array Human Interferons and Receptors (Cat# PAHS064ZC-12, Qiagen, Germantown, MD), RNA extraction (RNeasy Pls Mini kit ...
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bioRxiv - Neuroscience 2021Quote: ... and loaded into the Pig Inflammatory Cytokines & Receptors RT2 ProfilerTM PCR Array (Qiagen, Hilden, Germany). The qPCR reactions were performed using a Qiagen Rotor-Gene Q real-time cycler ...
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bioRxiv - Cell Biology 2023Quote: ... #3: 5’-AAGCATCGATAGGTAAGTTGA-3’ (SI03130008, Qiagen); #4 ...
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bioRxiv - Microbiology 2021Quote: ... Protein lysates were separated on NuPage 5-12% Bis-Tris or 3-8% Tris-Acetate gels (Novex, Qiagen) using MES running buffer (Novex ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was analyzed by immunoblotting using monoclonal anti-RGSH4 antibodies (Qiagen) to detect ABCG5 and polyclonal anti-hABCG8 antibodies (Novus Biologicals ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Cell Biology 2021Quote: ... si-USP36-3 (5’-CCGCAUCGAGAUGCCAUGCAU-3’; Qiagen), si-USP36-4 (5’-UUCCUUGUGAGUAGCUCUCAA-3’ ...
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bioRxiv - Cell Biology 2021Quote: ... si-USP10-3 (5’-AACACAGCUUCUGUUGACUCU-3’, Qiagen), si-USP10-4 (5’-AAGAACUAGUUCUUACUUCAA-3’ ...
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bioRxiv - Microbiology 2021Quote: ... tularensis IM protein SecY (Huntley, 2007) or the Penta-His HRP conjugate antibody (Qiagen).
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bioRxiv - Molecular Biology 2023Quote: ... 40% of the protein material of each fraction was analyzed by Western blot using an anti-his antibody (Penta-His antibody, Qiagen #34660). Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody ...
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bioRxiv - Cell Biology 2021Quote: ... E2D2/3 (5’-AACAGUAAUGGCAGCAUUUGU-3’) and E2D4 siRNAs (5’ – CCGAAUGACAGUCCUUACCAA-3’) all from Qiagen, USA(83).
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bioRxiv - Cancer Biology 2023Quote: ... HGF (5’-CTCACACCCGCTGGGAGTAC-3’, 5’-TCCTTGACCTTGGATGCATTC-3’), STAT3 (5’-CTTTGAGACCGAGGTGTATCACC-3’, 5’-GGTCAGCATGTTGTACCACAGG-3’) and B-Actin Primer Set (Qiagen, QT00095431). After preparing master mixes samples were prepared in quadruplicate in a 96-well Reaction Microplates (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... the proteins were transferred to nitrocellulose membranes before being revealed with anti-Histidine monoclonal antibodies (Qiagen). Immune complexes were detected with anti-rabbit peroxidase-conjugated secondary antibodies (Promega ...
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bioRxiv - Physiology 2020Quote: ... insulin-like growth factor 1 receptor (Igf1r) and sirtuin 1 (Sirt1) gene promoters were designed using the Pyromark Assay Deisgn 2.0 software (Qiagen). PCR and sequencing primers are provided in Supplementary Table 1 ...
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Fatty acid binding proteins shape the cellular response to activation of the glucocorticoid receptorbioRxiv - Pharmacology and Toxicology 2021Quote: ... qPCR was performed using the RT2 SYBR Green Mastermix and Glucocorticoid Receptor Signalling RT2 Profiler PCR Array from Qiagen, according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Proteins transferred to a nitrocellulose membrane were probed with a monoclonal anti-His6 antibody (Qiagen, Germantown, Maryland). Detection of proteins via Western blotting was performed by fluorescence detection using IR-Dye®-labeled fluorescent secondary antibodies and imaged using the Odyssey CLx Imager (LICOR Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bands were transferred to a nitrocellulose membrane and probed with primary antibodies [mouse anti-His (Qiagen) to detect CAT ...
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bioRxiv - Microbiology 2024Quote: ... His-tagged proteins were detected with a mouse anti-His HRP-conjugated antibody (1:3000) from Qiagen. MBP fusion proteins were detected with a rabbit anti-MBP from NEB company 1:1000 followed by an incubation with a goat anti-rabbit HRP-conjugated (1:3000) ...
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bioRxiv - Molecular Biology 2020Quote: ... total RNA was isolated from 1×106 BMMCs that were untreated or treated with IgE receptor crosslinking using the RNeasy mini kit (Qiagen). RNA was treated with DNase I according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... a second transfection of a 0.6 μg plasmid encoding either the C-terminal GFP11 tagged M2tail(368-466) or M2 receptor was performed using the Effectene Transfection Reagent (Qiagen). After washing each well with 2 mL PBS ...
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bioRxiv - Cell Biology 2023Quote: ... which is based on the expression of known ligand-receptor pairs112.Gene lists were imported into the IPA software Ingenuity Pathway Analysis (IPA) 01.12 (Qiagen Bioinformatics) to assess pathway/biological function enrichment analysis.
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bioRxiv - Neuroscience 2022Quote: ... All plasmids were verified by sequencing of the full receptor cDNA and purified with an EndoFree Plasmid Maxi Kit (Qiagen). cDNA sequences of all GPCR variants cloned in pcDNA3.1 are summarized in Data S2.
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bioRxiv - Cancer Biology 2024Quote: ... 5’-TGGTTCTACATCAGAGTTGTT-3’ (Qiagen). Lentiviral particles expressing SMART vectors doxycyclin-inducible shRNA were from Dharmacon as follows ...
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bioRxiv - Physiology 2020Quote: ... Erythrocytes were lysed for 3 minutes with 3 ml of EL buffer (Qiagen). After Fc blocking for 20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 3’mRNA-seq libraries were prepared using QIAseq UPX 3’ Transcriptome Kit (QIAGEN). In brief ...
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bioRxiv - Immunology 2021Quote: ... Gene expression profiling of inflammatory cytokines and receptors of normal and KP-OVA tumor bearing lungs was performed by custom RT2 Profiler PCR Array (Qiagen, cat. 330221) following manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... the 3×Flag-MCS fragments in LV3-SFFV-3×Flag-MCS-GFP (QZ35395, QIAGEN) was replaced by DreAM though NotI and SpeI sites to produce the LV3-SFFV-DreAM-GFP plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... siMad2 (5’-CUGAAAGUAACUCAUAAUCUA -3’) (Qiagen). For transfection of the scFv or scFvC plasmids ...
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bioRxiv - Microbiology 2021Quote: Recombinant protein was blotted with anti-His antibody using the same method: 1:2000 mouse anti-tetra-His IgG (Qiagen); 1:1500 goat anti-mouse IgG-HRP (Dako) ...
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bioRxiv - Cell Biology 2021Quote: ... Genomic DNA of clones showing depletion of spartin protein by immunoblot analysis with the spartin antibody were extracted (DNeasy Blood and Tissue Kit, Qiagen), and the genomic DNA sequence surrounding the target exon of spartin was amplified by PCR (sense ...
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bioRxiv - Microbiology 2023Quote: ... and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000, Qiagen) as primary antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... An A base was added to the 3’ ends with Klenow (3’-4’ exo-) (Qiagen). The processed ds-cDNA was then ligated to Illumina sequencing adapters with T4 DNA Ligase (Qiagen) ...
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bioRxiv - Genetics 2021Quote: ... followed by protein precipitation with the Protein Precipitation Solution (Qiagen) for 10 min at -20 °C ...