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Citations for Qiagen :
451 - 500 of 1051 citations for FABP2 I FABP Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was treated with RNase-free DNase I set (QIAGEN). RNA-seq library construction and RNA sequencing were performed by BGI Tech Solutions (Hong Kong).
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was carried out using the SYBR Green I (Qiagen) mix with primers targeted to U2 and U6 snRNA (Supplementary file 2) ...
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bioRxiv - Developmental Biology 2023Quote: ... with RNeasy Micro Kit with on-column DNase I digestion (QIAGEN). RNA concentration was measured by Qubit™ RNA High Sensitivity to calculate the amount of total RNA per cell ...
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bioRxiv - Microbiology 2022Quote: ... The genomic DNA was removed by on-column DNase I (Qiagen) treatment during the RNA extraction ...
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bioRxiv - Cell Biology 2024Quote: ... purified and treated with DNAse I using RNAeasy mini Kit (Qiagen). After quality evaluation by Experion kit and quantification by Qubit ...
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bioRxiv - Plant Biology 2024Quote: ... following the kit protocol with on-column DNase I digestion (Qiagen) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... (2010) using commercial antibodies directed against the His-tag following the instructions of the manufacture (Qiagen, Hilden, Germany).
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bioRxiv - Cell Biology 2021Quote: Antibodies and their conditions of use are as follows: mouse anti-His (Western blot, 1:1000; 34660; Qiagen), rabbit anti-Brl1 (Western blot ...
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bioRxiv - Microbiology 2020Quote: ... The 6 × his-tagged proteins were purified using a nickel-nitrilotriacetic acid (Ni-NTA) agarose matrix (30250, Qiagen). Cell-free extracts were loaded on 0.5 ml Ni-NTA matrixes and incubated on a roller shaker for 2 h at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged proteins were purified by Ni-affinity gravity-flow chromatography using the Ni-NTA agarose (Qiagen) following the product manual.
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged ubiquitin-conjugated proteins were purified by adding 100 µL of 50 % Ni-NTA agarose beads (Qiagen) equilibrated in A2 buffer to 1 mL of cleared extracts and incubated with rotation for 2-4 hr at room temperature ...
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SARS-CoV-2 comprehensive receptor profiling: mechanistic insight to drive new therapeutic strategiesbioRxiv - Cell Biology 2021Quote: ... each pre-incubated at a 2:1 molar ratio with Penta His Alexa Fluor 647 Conjugate (Qiagen, UK). Hits (duplicate AF647 positive spots ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His protein was purified from cell culture supernatant using a Ni-NTA (Qiagen) affinity column ...
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bioRxiv - Microbiology 2020Quote: A 6.5 nM concentration of His-tagged 16055 SOSIP.v8.3 trimer in TBS was added to a 96-well Ni-NTA plate (Qiagen) for 2 h at RT ...
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bioRxiv - Biochemistry 2019Quote: ... DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... His-SFP1 was purified using Ni-NTA affinity purification under native conditions using the standard manufacturer’s protocol (Qiagen). Recombinant His-SFP1 was used to immunize female New Zealand white rabbits (Covalab ...
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bioRxiv - Bioengineering 2020Quote: Initial small test purifications were performed using Ni-NTA Spin Column for His-Tagged proteins (Qiagen, Hilden, Germany) following the protocol for 6xHis-Tagged Proteins under Native Conditions from E ...
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bioRxiv - Biochemistry 2020Quote: ... The Ni-NTA agarose resin for purification of his-tagged proteins was purchased from Qiagen (Germantown, MD, USA). Superdex 75 16/600 and Superdex 200 Increase 10/300 size exclusion chromatography columns were purchased from GE Healthcare (Uppsala ...
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bioRxiv - Biochemistry 2021Quote: DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Microbiology 2023Quote: ... The N-terminal His-tagged Sif protein (Lmo0946-His6) was expressed and purified using Ni-NTA resin (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged talin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a gel filtration column (Superdex 200 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged vinculin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a Q-Sepharose ion exchange column ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% CA630) and rotated at 4°C for 2 hours before addition of His-beads (Ni-NTA, QIAGEN). After continuous rotating for another 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The agarose gel bound with the His-tagged recombinant protein was packed in the chromatography column (Qiagen, Germany), and the flow through was discarded ...
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bioRxiv - Plant Biology 2024Quote: ... All fractions were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting using anti-His (Qiagen) and anti-strep (MilliporeSigma ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA for ANXA2 (Human, Qiagen, Hs_ANXA2_8, SI02632385) or siRNA for Anxa2 (Mouse ...
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions followed by treatment with RNAse I (Qiagen) for 30 minutes at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was additionally treated with 30 units of DNase I (Qiagen) and an intron-spanning PCR product was checked on an agarose gel to exclude the possibility of DNA contamination ...
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bioRxiv - Microbiology 2022Quote: ... DNA was removed by DNase digestion (Qiagen RNase-Free DNase I Set), then in the same column the RNA was concentrated to 15 µL using the MN NucleoSpin RNA Clean-up XS (Macherey-Nagel) ...
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bioRxiv - Neuroscience 2020Quote: ... RNase-free DNAse I and RNeasy purification columns were purchased from Qiagen Inc ...
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bioRxiv - Cell Biology 2021Quote: ... or I-PpoI H98A mRNA was introduced with TransMessenger transfection reagent (Qiagen). 6 h post-mRNA transfection ...
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bioRxiv - Microbiology 2021Quote: ... the RNA sample was treated with RNase-free DNase I solution (Qiagen), twice ...
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bioRxiv - Plant Biology 2021Quote: ... a DNA digestion step was performed on-column with DNAse I (Qiagen). RNA quality was checked by using Experion HighSens capillary gels (BioRad ...
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bioRxiv - Plant Biology 2021Quote: ... and genomic DNA was removed by treating RNase-free DNase I (Qiagen). Extracted RNA was reverse-transcribed using oligo dT and SuperScript™ IV reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Genomic DNA was digested with DNase I (RNase-Free DNase Set, Qiagen). Concentration and purity of RNA were both evaluated with NanoDrop spectrophotometer ...
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Target of Rapamycin Complex 2 modulates development through Hedgehog/Patched signaling in C. elegansbioRxiv - Developmental Biology 2022Quote: ... Samples were lysed using metal beads and the Tissuelyser I system (Qiagen).
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bioRxiv - Microbiology 2019Quote: ... according to the manufacturer’s instructions with on-column DNase I digestion (Qiagen). The RNA extracts were aliquoted and stored at - 80°C.
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bioRxiv - Neuroscience 2019Quote: ... Purified RNA was inbcubated with DNase I (RNase-Free DNase Set, Qiagen) over a mini kit column (RNAeasy ...
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bioRxiv - Genetics 2020Quote: ... Total RNAs were treated with amplification-grade RNase-free DNase I (Qiagen) to remove contaminating DNAs ...
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bioRxiv - Genetics 2020Quote: ... with additional DNase I treatment or using QuantiTect Reverse Transcription Kit (QIAGEN). SYBR Green based real-time qPCR (Sybr Green I Master ...
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bioRxiv - Biochemistry 2020Quote: ... Crystallization screening was performed at 4°C with JCSG core I (Qiagen) using the sitting drop vapor diffusion method ...
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bioRxiv - Developmental Biology 2022Quote: ... with 15 minute on-column digestion with RNase-free DNase I (Qiagen) to eliminate genomic DNA ...
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bioRxiv - Microbiology 2023Quote: ... On-column DNase I treatment using the RNase-free DNase set (Qiagen) was included in the RNA extraction to remove any DNA contaminants ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was removed from samples by RNase-free DNase I (Qiagen) treatment ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The digestion of gDNA was performed by addition of DNase I (Qiagen) and the concentration of total RNA was measured using the Qubit 2.0 Fluorometer (Thermo Fisher) ...
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bioRxiv - Cell Biology 2022Quote: ... and subjected to an additional DNA digestion step (Qiagen DNase I kit). Reverse transcription (1 μg RNA per sample ...
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bioRxiv - Neuroscience 2023Quote: ... DNase digestion was performed on the column membrane using DNase I (Qiagen) for 15 min at room temperature according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Pre-miRNAs were cleared from the DNA template using DNase I (QIAGEN) for 15 min and 20 µl of GLB II (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The genomic DNA was removed using on-column DNase-I digestion (Qiagen) for 15 min ...