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Citations for Qiagen :
401 - 450 of 952 citations for Dl 4 Methoxyestradiol 13 14 15 16 17 18 13C6 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... siRNAs were selected among FlexiTube GeneSolution 4 siRNA sets (Qiagen) and reordered after validation as dTdT-overhanging 19 nt RNA duplexes (Thermo) ...
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bioRxiv - Microbiology 2020Quote: ... 4) Powersoil® Isolation kit (MO Bio Laboratories/Qiagen, Canada) by the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and subsequently normalized to 4 nM using elution buffer (Qiagen) with 0.1% Tween20 (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... whole ticks were bisected using a sterile 16-gauge needle and incubated in a lysis buffer (i.e., 180 μl Qiagen Buffer ATL and 20 μl proteinase K) at 56°C overnight ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from a subset of 15 samples using the RNA PowerSoil Total RNA isolation kit (Qiagen). Soil samples (8 g ...
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bioRxiv - Immunology 2021Quote: ... and low molecular weight DNA isolated for downstream analysis using a 1.2x volumes AMPureXP SPRI-beads and eluted in 15 μl EB buffer (Qiagen). ATAC-seq libraries were amplified using 5 μl each of the i5 and i7 Nextera Indexing primers and 25 μl of 2x HiFi HotStart ReadyMix (Roche Diagnostics ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was isolated from mycelia on a Biosprint 15 instrument using Biosprint DNA Plant Kit (QIAGEN, Hilden, Germany) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... in a 15 ul reaction volume in 96-well plates or with the Repli-g Single Cell kit (Qiagen) in a 10 ul reaction volume in 384-well plates ...
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bioRxiv - Developmental Biology 2021Quote: Pools of twenty 15 dpf-embryo (zmiz1a+/- and zmiz1a-/-) were used for total RNA isolation with RNAeasy columns (Qiagen) from five independent crosses ...
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bioRxiv - Plant Biology 2021Quote: ... Reactions were carried out in 15 μL total reaction volume: 7.5 μL of SYBR-green (QuantiFast SYBR Green PCR Kit - Qiagen), 0.3 μL of forward and reverse gene-specific primers ...
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bioRxiv - Neuroscience 2020Quote: ESR1 expression was manipulated with custom-designed locked nucleic acid (LNA™) 15-mer antisense oligonucleotides designed by Qiagen following Kelly & Goodson.41 The antisense oligo for ESR1 knockdown (ESR1-KD ...
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bioRxiv - Genomics 2022Quote: ... and 15-20 mg were used to extract RNA using a combination of Trizol and RNeasy Micro Kit (Qiagen), followed by a DNase removal step according to the manufacturer ...
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bioRxiv - Developmental Biology 2020Quote: ... using the QIAGEN TissueLyser at 15 Hz for 2-3 min with a Stainless-Steel Bead (QIAGEN catalog # 69989). Phase separation was induced with chloroform ...
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bioRxiv - Immunology 2020Quote: ... cells were first washed and pelleted five times with 15 ml of PBS then lysed with QIAzol reagent (Qiagen), RNA was extracted according to manufactures directions ...
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bioRxiv - Plant Biology 2023Quote: RNA was extracted from the 15-day-old plants of different genotypes using the Plant Easy Mini Kit (Qiagen). The cDNA was obtained from RNA samples by using the Transcriptor First Strand cDNA Synthesis Kit (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmids were allowed to air dry in a fume hood for 15 minutes before resuspension in 500µL Buffer TE (Qiagen). Plasmid concentration was quantified using a Nanodrop 2000.
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bioRxiv - Plant Biology 2023Quote: ... The prepared libraries were enriched using 15 cycles of PCR and purified by the QIAquick PCR purification kit (Qiagen). The Agilent 2100 Bioanalyzer was used to control the size and purity of the samples using the Agilent High Sensitivity DNA Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 µl NEB2 buffer and 0.1 U enzyme were incubated 15 min 30°C and purified from unincorporated nucleotides using Nucleotide Removal Kit (Qiagen). Binding reactions were carried out in a total volume of 10 µl ...
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bioRxiv - Bioengineering 2024Quote: ... spun down for 15 s at 8,000 g and eluted 3 times by adding 700 µL Buffer RW1 (Qiagen) and spinning ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR mix contained 0.16 µM of each of the fluorescent universal primer and of the reverse specified primer and 0.04 µM of the 5’ tail forward primer in a final 15 µl reaction volume (2x QIAGEN Multiplex PCR Master Mix with 3 mM Mg2+ ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA from 15 post-operative endopthalmitis isolates was extracted using a DNeasy Blood and Tissue Kit (Qiagen, Germantown, MD) from 1mL bacterial cultures grown in Brain Heart Infusion media ...
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bioRxiv - Microbiology 2020Quote: Oral fluid samples were prepared by homogenization of 200 μl sample (30 sec at 15 Hz) in a Tissuelyser II (Qiagen) followed by centrifugation (2 min at 10.000 rpm) ...
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bioRxiv - Cancer Biology 2021Quote: Protein lysate [1μg/μL] was RNase A digested at 37°C for 15 min following addition of 1 μg RNase A (Qiagen) per 25 μg protein ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from MSCs cultured for 3 or 15 days under different experimental conditions using RNeasy Micro Kit (Qiagen). RNA quantity and integrity was measured with a NanoDrop 1000 (ThermoScientific) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The tissue was ground to a fine powder by adding 10-15 zirconia beads (1 mm diameter) and using a TissueLyser (Qiagen) for sunflower ligules ...
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bioRxiv - Genetics 2019Quote: ... or 0.1% DMSO control (n = 15) in the absence of H2O2 for 24 hours and then processed for RNA isolation with the RNeasy kit (Qiagen). Reverse transcription (RT ...
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bioRxiv - Developmental Biology 2020Quote: ... In vitro transcribed mRNAs were DNAse-treated using TURBO-DNAse for 15 min at 37°C and purified using the RNeasy Mini Kit (Qiagen) and quantifed using Qubit™ RNA BR Assay Kit (ThermoFisher ...
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bioRxiv - Immunology 2019Quote: ... 6µL ethanol/phosphate buffer (85:15 v/v ethanol/10mM phosphate buffer) were added and the tissue was homogenized (TissueLyser II, Qiagen). Samples were centrifuged at 5000g for 5 minutes at 4°C and the supernatants were transferred to a fresh tube and used for analyses ...
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bioRxiv - Cancer Biology 2019Quote: ... The lysate was then centrifuged at 15 000 ×g and the supernatant was added to the 1 mL Ni-NTA resin (Qiagen). After washing with Buffer A supplied with 30 mM imidazole ...
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bioRxiv - Physiology 2019Quote: ... 72 °C - 10 min) and amplicons separated using a Qiaxcel Advanced Separation System using 15 - 3000 bp markers (Qiagen, UK).
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bioRxiv - Genetics 2021Quote: Amplified libraries were then cleaned by pooling 25 uL per well into a 15 mL conical tube and cleaned via a Qiaquick PCR purification column following manufacturer’s protocol (Qiagen 28106). The pooled sample was eluted in 50 uL 10 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2020Quote: ... spin column and proper buffer washing steps were carried out following manufacturer recommendations including a 15 min on-column DNAse (Qiagen, ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Imidazole pH 8.0 was added to the detergent phase to a final concentration of 15 mM and the mixture was incubated with Ni-NTA agarose beads (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Cultures were spun at 3500 x g for 15 min and RNA was extracted from the bacterial pellet with QIAzol lysis reagent and the miRNeasy Mini Kit (Qiagen). rRNA was removed with the Bacterial RiboMinus Transcriptome Isolation Kit (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 % (v/v) glycerol) for 30 s at a frequency of 15 s-1 using a TissueLyser II (QIAGEN). As the tissue homogenates were to be used for both native and SDS PAGE ...
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bioRxiv - Biophysics 2022Quote: ... The lysates were centrifuged (15 min, 27000 ×g) and the supernatants were applied to gravity flow columns packed with Ni-NTA Superflow (QIAGEN). The columns were washed with buffers (20 mM NaH2PO4 ...
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bioRxiv - Biophysics 2022Quote: ... before rapidly cooling the cell suspension on ice.22 The lysate was centrifuged (15 min, 27000 g) and the supernatant was applied to gravity flow columns packed with Ni-NTA Superflow (QIAGEN). The columns were washed with buffers (20 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2022Quote: Pellets were thawed and lysed on ice in 15 mL lysis buffer containing freshly added lysozyme and benzonase prepared according to manufacturer instructions (Qiagen Qproteome Bacterial Protein Prep Kit ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were collected via centrifugation at 2500 ×g for 15 min and used for DNA purification using the DNeasy Blood & Tissue Kit (Qiagen), following the optional protocol for gram-negative bacteria provided by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... from the top A horizon were immediately collected for DNA extractions in 15 mL tubes containing 5 mL Lifeguard Soil Preservation Solution (QIAGEN) using chilled soil-processing trays ...
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bioRxiv - Cancer Biology 2022Quote: ... shRNA expressing cells were isolated by fluorescence activated cell sorting at passage doubling 1 and 15 followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Sequencing libraries were prepared by subjecting 18 μg (1.2 μg x 15 reactions ...
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bioRxiv - Genomics 2020Quote: ... The ethanol was then poured out and the pellet was air-dried for 15 minutes before resuspension in 50 to 100 μL of DNA Hydration Solution (Qiagen). DNA was sequenced with paired end Illumina sequencing at 30x coverage.
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bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was transferred to a 15 ml falcon tube and 1 ml of a 50% slurry consisting of NiNTA agarose beads (Qiagen) in resuspension buffer was added per 10 ml of lysate ...
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bioRxiv - Microbiology 2021Quote: ... DNA was purified from a homogenate of a whole single adult female cricket prepared by 15 min beating with a lead bead using the MagAttract HMW DNA Kit (Qiagen) according to manufacturer specifications ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total genomic DNA was extracted from ethanol-preserved fin tissues using the BioSprint 15 kit for tissue and blood (Qiagen). Sequences of the mitochondrial gene ...
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bioRxiv - Microbiology 2021Quote: ... and 15-30 μg of DNA was extracted from ethanol-fixed fish tissues using a DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer’s guidelines.
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bioRxiv - Immunology 2020Quote: ... Supernatants were removed and the remaining cell pellet (500 μl) was transferred into a 15 ml tube containing 2.5 ml RNAprotect® Cell Reagent (Qiagen). The cells were resuspended by vortexing ...
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bioRxiv - Immunology 2020Quote: ... cleaning were used to purify 100 μl of pooled cDNA with final elution in 15 μl PCR-grade H2O (Qiagen). After quantification with Qubit dsDNA HS assay (Thermofisher) ...
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bioRxiv - Immunology 2021Quote: ... and subsequent crystallization optimization was performed manually using hanging-drop vapor diffusion method with 15-well screw-cap crystallization plates (Qiagen). The H5.28-HA head complex was crystallized in 7-9% PEG 8000 ...
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bioRxiv - Biochemistry 2022Quote: ... Imidazole pH 8.0 was added to the detergent phase to a final concentration of 15 mM and the mixture was incubated with Ni-NTA agarose beads (Qiagen) for 1 hour at 4°C ...