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Citations for Qiagen :
1 - 50 of 1445 citations for Dengue Virus Serotype 3 DIII envelope protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... To sequence the envelope from serum virus we isolated viral RNA (Qiagen), synthesized cDNA ...
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bioRxiv - Microbiology 2022Quote: ... A wild-type virus stock NL4-3 was prepared by transfection of the pNL4-3 plasmid (purified using the Qiagen Plasmid Maxikit) into HeLa cells ...
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bioRxiv - Microbiology 2020Quote: ... Virus RNA virus was extracted by Qiamp viral RNA (Qiagen) and quantified using Qbit 3 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: TaqMan RT-qPCR targeting the envelope protein gene (E gene) was performed using the Quantitect Probe RT-PCR kit (Qiagen, Courtaboeuf, France). The model of primers/probe used was as follows ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA from the original material used for next-generation sequencing of the serum and passage 3 cultured MRI103 virus was used as the template for amplification using the One-Step Ahead RT-PCR kit (Qiagen).
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bioRxiv - Microbiology 2023Quote: ... round 1 and 3 virus infections of GeCKO-A549 cells using the midi gDNA extraction kit (Qiagen, Germantown, MD, USA). The sgRNA’s DNA copies were PCR amplified from the extracted gDNAs for next generation sequencing (Fig 1) ...
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bioRxiv - Microbiology 2024Quote: ... using Virus Extraction Mini Kit (Qiagen). The RT-qPCR assay were performed with a SuperScript III Platinum One-Step RT-qPCR Kit with ROX (Invitrogen - Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... with EZ1 DSP Virus kit (Qiagen).
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bioRxiv - Physiology 2021Quote: ... We infected Sf9 cells with the recombinant virus to express and purify the His-tagged proteins using a nickel-nitrilotriacetic acid (Ni-NTA, Qiagen) column ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from virus supernatants using QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). qPCR was then performed using AgPath RT-PCR (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... RNA was extracted from SIV and SIVΔNef virus stocks with the QIAamp UltraSens Virus kit (Qiagen, Germantown, MD), reverse transcribed with the Superscript VILO kit (Thermofisher ...
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bioRxiv - Immunology 2021Quote: ... ZIKA Viral RNA was isolated from Zika virus stock using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704) to create a standard curve using serial 10-fold dilutions of ZIKV RNA ...
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bioRxiv - Microbiology 2022Quote: ... copies were extracted from plasma virus using the Qiagen BioRobot EZ1 Workstation with EZ1 Virus Mini Kit v2.0 (Qiagen). Eluted vRNA was subsequently used as a template for cDNA synthesis and reverse-transcribed using the reverse primer SHIV.Env.R1 (5’-TACCCCTACCAAGTCATCA-3’ ...
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bioRxiv - Genetics 2020Quote: ... Total viral RNA was extracted from patient specimens and tested for the presence of SARS-CoV-2 with an EZ1 virus extraction kit and EZ1 Advanced XL instrument with the Virus card (Qiagen), or QIASymphony DSP Virus kit and QIASymphony instrument (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... For next generation sequencing RNA from virus-containing samples were extracted using the QIAsymphony DSP Virus/Pathogen mini kit (Qiagen). RNA was DNase-treated using the TURBO-free Kit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Whole genome sequencing of the isolated virus was done isolating viral RNA using the QIAamp MinElute Virus Spine kit (Qiagen) and performing Illumina sequencing ...
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bioRxiv - Genetics 2022Quote: ... The QIAamp MinElute Virus Spin Kit (QIAGEN, Germany) was used for viral nucleic acid extraction ...
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bioRxiv - Microbiology 2023Quote: ... Digested protein was mixed with 3 ml of NTA super-flow resin (Qiagen) that had been pre-equilibrated in wash buffer ...
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bioRxiv - Genetics 2020Quote: ... or QIASymphony DSP Virus kit and QIASymphony instrument (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... QIAamp MinElute virus spin kit (Qiagen Sciences, Maryland, MD) was used to extract total nucleic acids from each wastewater sample as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and a dedicated kit (QIAsymphony DSP Virus/Pathogen, Qiagen). All the extracts were stored at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... Equine arteritis virus (EAV) in AVL lysis buffer (Qiagen) was spiked into the reagent as internal control for extracellular RNA samples ...
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bioRxiv - Microbiology 2021Quote: ... Virus RNA was extracted by Qiamp viral RNA (Qiagen) and quantified using Qbit 3 Fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... using the “QIAamp Min ELUTE Virus Spin” Kit (QIAGEN), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... using the QIAamp 96 Virus kit QIAcube HT (Qiagen). To detect SARS-CoV-2 RNA ...
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bioRxiv - Microbiology 2024Quote: ... supernatants with the QIAamp MinElute Virus Spin Kit (Qiagen) as per manufacturers’ instructions ...
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bioRxiv - Microbiology 2024Quote: ... viral RNA was extracted from the challenge virus inoculum and virus-positive clinical samples using the QIAamp viral RNA mini kit (Qiagen, Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Recombinant protein was captured by overnight incubation with 3 ml Ni-NTA resin (Qiagen) followed by washing with 30 ml denaturing buffer (8 M urea ...
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bioRxiv - Microbiology 2022Quote: ... and QiaAmp 96 Virus QiaCube HT kit (Qiagen, Cat. 57731). RT-PCR reaction was setup using SensiFast Probe No-ROX One- Step Kit (Bioline ...
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bioRxiv - Immunology 2024Quote: ... or QIASymphony DSP virus/pathogen Midi kit (Qiagen, cat. 937055) on a QIASymphony SP laboratory automation instrument platform according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Specific enrichment of nuclear proteins from astrocytes and neurons (NS, SD, 3 biological replicates) was performed using a Qproteome Nuclear Protein Kit (QIAGEN, Cat # 37582) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... from mouse plasma using the QIAamp MinElute Virus Spin Kit (Qiagen) along with a DNaseI (Qiagen ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted using the QIAamp MinElute Virus Kit (Qiagen, #57704). Purified DNA was eluted in 50μl of Ultra-Pure Water (UltraPure™ DNase/RNase-Free Distilled Water ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted using QIASymphony DSP Virus Pathogen Midi kit (Qiagen) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Virus was inactivated by mixing 1:1 with Buffer ATL (Qiagen) prior to viral RNA extraction from NP swabs ...
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bioRxiv - Microbiology 2020Quote: ... and a plasmid expressing VSV-G or HIVKB9 envelope glycoproteins were cotransfected at the mass ratio of 9:1 (9 Hi.fate / 1 Env) using Effectene (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: 293FT cells were transfected with pMSCV-dnCRTC or pMSCV-GFP constructs together with packaging plasmid pMD.MLV and pseudotyped envelope plasmid pMD2-VSV-G using Effectene transfection reagent (Qiagen #301425) as previously described ...
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bioRxiv - Zoology 2021Quote: Nucleic acids were extracted using a QIAamp MinElute Virus Spin Kit (QIAGEN) and used to construct the sequencing libraries ...
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bioRxiv - Plant Biology 2021Quote: ... Virion DNA was isolated using QIAamp MinElute Virus Spin Kit (Qiagen, Maryland).
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bioRxiv - Immunology 2020Quote: Plasma RNA was extracted using the QIAamp MinElute Virus Spin Kit (Qiagen) along with a DNaseI (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR of SIV gag RNA was by QuantiTect Virus kit (Qiagen 211011) or ddPCR using One-Step RT ddPCR Advanced Kit for Probes (Bio-Rad 1864022) ...
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bioRxiv - Microbiology 2023Quote: ... and recLI9-NS1-GFP11 reporter virus was purified using RNeasy kit (Qiagen). cDNA synthesis was performed as above using random hexamer primers (25°C for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0, Qiagen). Then ...
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bioRxiv - Microbiology 2022Quote: ... qPCR of SIV gag RNA was by QuantiTect Virus kit (Qiagen 211011) or ddPCR using One-Step RT ddPCR Adv kit (Bio-Rad 1864022) ...
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bioRxiv - Cell Biology 2023Quote: ... #3: 5’-AAGCATCGATAGGTAAGTTGA-3’ (SI03130008, Qiagen); #4 ...
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bioRxiv - Microbiology 2021Quote: ... Protein lysates were separated on NuPage 5-12% Bis-Tris or 3-8% Tris-Acetate gels (Novex, Qiagen) using MES running buffer (Novex ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from virus stock using QIAamp Viral RNA Mini Kit (Qiagen) and treated with TURBO DNase (Ambion) ...
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bioRxiv - Neuroscience 2020Quote: SIV RNA was measured by qRT-PCR using the QuantiTech Virus Kid (Qiagen) and the following primers and probes in the SIV gag region (Fwd ...
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bioRxiv - Microbiology 2020Quote: ... RNA of single virus clone was extracted by Viral RNA Mini Kit (QIAGEN) and reversely transcribed using PrimeScriptTM II 1st Strand cDNA Synthesis Kit (TaKaRa ...
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bioRxiv - Microbiology 2020Quote: ... virus media was removed and cells were harvested using RNAprotect Cell Reagent (Qiagen). RNA was extracted using RNeasy columns (Qiagen ...