Labshake search
Citations for Qiagen :
101 - 150 of 2308 citations for Dengue Virus Serotype 1 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... using a QIAamp Mini Elute Virus spin kit for the viral RNA/DNA extraction (QIAGEN, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Qiasymphony automated platform was used to isolate total cellular RNA (DSP virus/pathogen mini kit (Qiagen). RNA was further processed in an in-house assay using primers of previously validated assays (44 ...
-
bioRxiv - Immunology 2020Quote: Total RNA was extracted from the virus sample with a QIAamp Viral RNA mini kit (QIAGEN). SARS-CoV-2 RdRP-2 gene primer probes sequences are as follows ...
-
bioRxiv - Microbiology 2020Quote: DNA was extracted from each sample using the QIAamp MinElute Virus Spin Kit (Qiagen, Hilden, Germany), and a semi-nested PCR assays was used for the detection of viral nucleic acids in each sample ...
-
bioRxiv - Genomics 2021Quote: ... Remaining 140 µL of concentrated virus was processed according to QIAamp Viral RNA Mini kit (QIAGEN) protocol ...
-
bioRxiv - Microbiology 2023Quote: Nucleic acids were extracted from specimens using QIAamp 96 Virus QIAcube HT Kit (Qiagen, Hilden, Germany), QIAamp Viral RNA Mini Kit (Qiagen) ...
-
Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: Total DNA was extracted from tissues using QIAamp MinElute Virus Spin Kit (57704, Qiagen, Venlo, Netherlands) or KingFisher Cell and Tissue DNA kit (97030196 ...
-
bioRxiv - Microbiology 2024Quote: ... following the manufacturer’s protocol for the QIAamp MinElute Virus Spin Kit (Cat. 57704, Qiagen, Hilden, Alemania). The resulting DNA for each sample was quantified with a Qubit fluorometer (Cat ...
-
bioRxiv - Plant Biology 2021Quote: ... Protein extract (1 mg) was incubated with His6-RabD2c (1 μg) and Ni-NTA Agarose (QIAGEN) for 1 h at 4°C ...
-
bioRxiv - Physiology 2021Quote: RNA was isolated from the three HEK293 cell lines after 48 h in culture using the RNeasy Protect Mini Kit (catalog #74124, Qiagen). After reverse transcription (Super-Script II reverse transcriptase ...
-
bioRxiv - Neuroscience 2021Quote: ... HEK293 cells were transfected with plasmids (WT mGlu1, the mGlu1 mutants, or the control vector) using SuperFect transfection reagent (Qiagen) or Viafect (promega ...
-
bioRxiv - Cell Biology 2021Quote: Total mRNA was isolated from HEK293 (2×106) and Jurkat cells (4×106) parental and MCU-KO cell lines using the RNeasy Mini Kit (Qiagen). Isolated RNA was then analyzed using a NanoDrop spectrophotometer (Thermo Scientific ...
-
bioRxiv - Microbiology 2019Quote: ... Approximately 5×106 HEK293 cells were used to isolate RNA with the All Prep RNA/DNA Mini Kit (Qiagen; 80204). cDNA was generated using 1μg of RNA with oligo(dT ...
-
bioRxiv - Genomics 2020Quote: The DNA of VLPs was extracted following the manufacture’s protocol for the QIAampMinElute Virus Spin Kit (QIAGEN). The resulting DNAs were quantified (Qubit ...
-
bioRxiv - Microbiology 2021Quote: DNA was extracted from the sucrose-purified virus using the Qiagen DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was extracted from mock and virus infected Vero cells using the RNeasy mini kit (Qiagen, Germany). Next ...
-
bioRxiv - Plant Biology 2020Quote: DNA from virus-infected plant tissues was extracted by DNeasy Plant Mini Kit (QIAGEN, Cat. No. 69104), and 500 ng of purified DNA was subjected to bisulfite treatment using EpiTect Plus DNA Bisulfite Kit (QIAGEN ...
-
bioRxiv - Microbiology 2022Quote: The viral RNA of the egg-passaged virus was extracted using QIAamp Viral RNA Mini Kit (Qiagen). The extracted RNA was then reverse transcribed to cDNA using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) ...
-
bioRxiv - Immunology 2021Quote: ... Viral RNA from mice serum was extracted using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... For quantification RNA was extracted from 100 μl of virus preparation using RNeasy kit (Qiagen, Manchester, UK) and analysed by reverse transcription and quantitative PCR (qPCR).
-
bioRxiv - Bioengineering 2020Quote: ... Viral RNA (vRNA) was isolated from blood plasma using the Qiagen QIAmp UltraSense Virus Kit (Qiagen #53704) in accordance with manufacturer’s instructions for 0.5 mL of plasma ...
-
bioRxiv - Microbiology 2020Quote: ... RNA was extracted from cell-free virus-containing supernatants using the viral RNA extraction mini kit (Qiagen). cDNA synthesis was conducted using Superscript IV and the uni12-FluG primer (AGCGAAAGCAGG) ...
-
bioRxiv - Immunology 2021Quote: ... RNA was isolated from 200 μL sample using the QIAamp MinElute Virus spin kit (Qiagen, Frederick, MD). For the qRT-PCR assay ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The RNA from the escaped virus was isolated using a QIAamp viral RNA mini kit (Qiagen, #52904), and the full-length HA was amplified using gene-specific primers and the OneStep RT-PCR kit (Qiagen ...
-
bioRxiv - Microbiology 2022Quote: Total virus-RNAs were extracted from SK.N.SH infected cells at MOI 0.5 using RNeasy minikit (Qiagen, #74104). ISRE reporter assay was performed in STING37 cells plated in 24 wells plate (2×105 cells/well ...
-
A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from the concentrated virus with the QIAamp Viral RNA Mini Kit (Qiagen, 52904). The sgRNA sequences found in the genomic DNA and viral RNA samples were amplified by PCR (Agilent ...
-
bioRxiv - Genomics 2022Quote: Viral RNA was extracted from 200 μl of clinical sample using QIAamp 96 Virus QIAcube HT (QIAGEN) according to the manufacturer’s instruction ...
-
bioRxiv - Bioengineering 2022Quote: ... Viral RNA (vRNA) was isolated from blood plasma using the Qiagen QIAmp UltraSense Virus Kit (Qiagen #53704) in accordance with manufacturer’s instructions for 0.5 mL of plasma ...
-
bioRxiv - Microbiology 2023Quote: ... and cleared supernatants stored at −80°C until virus titration/ spin through a QIA shredder column (Qiagen). Viral titers were expressed as TCID50 units per gram source tissue.
-
bioRxiv - Cell Biology 2024Quote: ... into 4 million HEK293-GP cells in 300 µl Buffer EC with 16 µl Enhancer and 60 µl Effectene Transfection Reagent (Qiagen 301425) (Morgenstern and Land ...
-
bioRxiv - Immunology 2022Quote: ... The cleaved protein was passed over a 1 mL Ni-NTA agarose (Qiagen) gravity column to remove TEV protease ...
-
bioRxiv - Molecular Biology 2023Quote: The eluted protein was mixed with 1 ml Strep-Tactin Superflow agarose (Qiagen) and incubated at 4 °C for 30 min ...
-
bioRxiv - Microbiology 2020Quote: ... Total nucleic acids were extracted using QIAamp 96 virus Qiacube HT kit on QIAxtractor Automated extraction (Qiagen, US) following the manufacturer’s instructions.
-
bioRxiv - Immunology 2021Quote: The virus RNA was extracted from clinical samples using the QIAamp viral RNA mini kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: Viral RNA from cell culture supernatants was isolated as the manufacturer’s instructions using QIAamp MinElute Virus kit (Qiagen). Cells were suspended in TRI Reagent (Sigma Aldrich) ...
-
bioRxiv - Microbiology 2020Quote: Load Viral RNA was isolated from plasma and cerebral spinal fluid (CSF) using the QuantiTech Virus Kit (Qiagen). Viral loads were quantified by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR ...
-
bioRxiv - Biochemistry 2021Quote: ... RNA was extracted from 200 µl of the inactivated supernatant using the EZ1 Virus Mini Kit (Qiagen, Germany). As described previously ...
-
bioRxiv - Immunology 2021Quote: ... RNA was extracted from nasopharyngeal swab samples on the QiaSymphony platform using the Virus Pathogen Mini Kit (Qiagen). Libraries were prepared using the Illumina DNA Flex library preparation kit and sequenced on an Illumina MiSeq (V2 ...
-
bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from 140 µl of virus stock (SARS-CoV-2 VOC Delta, GISAID ID: EPI_ISL_15250227) using a QIAamp Viral RNA Mini Kit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: RNA from virus stocks was extracted with the QIAamp Viral RNA Mini Kit (Cat# 52904, QIAGEN, Hilden, Germany), and one-step cDNA synthesis was then performed with SuperScript IV RT Viral cDNA (Cat# 12594025 ...
-
bioRxiv - Immunology 2022Quote: ... 200 μL of nasal swab sample was extracted with the QIAamp 96 Virus QIAcube HT kit (Qiagen, Germany) on the QIAcube HT System (Qiagen ...
-
bioRxiv - Microbiology 2023Quote: ... viral RNA was extracted from the virus stocks using the QIAamp viral RNA mini kit (Qiagen, Cat#74l36) and viral genomes were sequenced as described before [31].
-
bioRxiv - Microbiology 2023Quote: Virus isolates and respiratory specimens were extracted using the QIAgen Viral RNA Mini-Kit (QIAgen, Inc., Valencia, CA). Input sample volume was 140 μl ...
-
bioRxiv - Microbiology 2020Quote: ... and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen) and linear acrylamide (ThermoFisher Scientific ...
-
bioRxiv - Epidemiology 2019Quote: Total RNA was extracted from SFTS virus IgM positive samples using a QIAamp viral RNA mini Kit (Qiagen, Germany), following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: Viral RNA (vRNA) was isolated from virus-infected lung homogenate supernatant using the QIAamp Viral RNA Mini Kit (QIAGEN). Influenza genome copies were quantified from RNA isolations by reverse transcription and quantitative PCR (RT-qPCR ...
-
bioRxiv - Biophysics 2021Quote: The SARS-CoV-2 RNA was isolated from virus particles with the QIAmp viral RNA mini kit (Qiagen - USA) and reversely transcribed to cDNA with the High-Capacity Reverse Transcription Kit (Thermo - USA) ...
-
bioRxiv - Microbiology 2020Quote: ... followed by extraction of viral nucleic acids using a commercial kit (QIAamp MinElute virus spin kit, Qiagen, Venlo, Netherlands). A portion (2.5 µl ...
-
bioRxiv - Microbiology 2021Quote: ... the resulting filtrate was used for viral DNA extraction using the QiAmp Ultra-Sensitive Virus Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol.
-
bioRxiv - Immunology 2022Quote: ... Nucleic acid was first extracted from each blood sample using QIAamp MinElute Virus Spin kits (Qiagen, Mississauga, Ontario, Canada) according to the manufacturer’s instructions ...