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Citations for Qiagen :
1 - 50 of 1536 citations for D Iditol 1 13C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... and 1 μM Locked Nucleic Acid (LNA) oligo-d(T)30 (Qiagen) in primary-probe hybridization buffer composed of 40% formamide (Sigma ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from 0.25 g of soil from all segments from two of the five 13C soil cores using the DNeasy PowerSoil DNA Extraction kit (Qiagen, Hilden, Germany) to determine the segment with the highest abundance of methanogens or methanotrophs based on the respective functional marker genes using qPCR as described below ...
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bioRxiv - Plant Biology 2022Quote: ... and 200 µM VLCFA mix for 1 d using the RNeasy Plant kit (QIAGEN, Hilden, Germany). For isolation of RNA from the bending site of roots ...
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bioRxiv - Developmental Biology 2022Quote: ... Oocytes and embryos were then washed with D-PBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...
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bioRxiv - Plant Biology 2021Quote: RNA was extracted from root tips (1-2 mm) of 7-d-old seedlings using the RNEasy plant mini kit (QIAGEN), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... and (d) Purify DNA using MinElute column (Qiagen) as directed by manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... ONTARGETplus Non-targeting siRNA oligo#1 (NT1) 5’-TGGTTTACATGTCGACTAA-3’ (Dharmacon, D-001810-01) and FlexiTube siRNA h USP31 oligo #1 (Q1) 5’-CCGAGTTCATGAAGACCTCAA-3’ (Qiagen, SI00758422), oligo #2 (Q2 ...
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bioRxiv - Molecular Biology 2021Quote: ... was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) into the LB media and purified by Ni-NTA affinity chromatography (Qiagen, USA). The full length of ASSCP2 protein (ASSCP2-R ...
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bioRxiv - Genomics 2021Quote: ... total RNA was extracted using RNeasy kit (QIAGEN, D usseldorf, Germany) from between 5 ×106 and 10×106 Z-138 ...
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bioRxiv - Cell Biology 2024Quote: Cells were washed once with D-PBS and lysed using QIAzol reagent (QIAGEN). Total RNA was extracted using the Direct-zol RNA Miniprep kit (Zymo Research) ...
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bioRxiv - Immunology 2021Quote: ... D from four matched BM samples using the RNeasy Mini Kit (Qiagen, Inc. Valencia, CA) by following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted combining a lysozyme method and the D-neasy blood tissue kit (Qiagen). The product was purified by the PCR purification kit (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... according to the method described in appendix D of the Qiagen RNeasy mini kit manual (Qiagen, 74104). RNA concentrations were determined using a Nanodrop one instrument (Thermofisher Scientific).
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bioRxiv - Plant Biology 2022Quote: ... Whole seedlings were harvested after 9 d and total RNA was extracted with RNeasy Plant Mini Kit (QIAGEN) following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2021Quote: Total brain RNA from 21 days old Itm2bD/D and Itm2bww/ rats (2 male and 2 females per each genotype) was extracted with RNeasy RNA Isolation kit (Qiagen). Standard RNA-Seq procedures and data analysis was performed by Genewiz following proprietary methods (https://cdn2.hubspot.net/hubfs/3478602/NGS/RNA-Seq/GENEWIZ_RNA-Seq_Technical_Specifications_US.pdf) ...
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bioRxiv - Microbiology 2020Quote: ... and Marine Benthic Group – D in the Thermoplasmata (with the latter for core 32 only) using the Quantifast SYBRGreen kit (Qiagen) on a BioRad Opticon2 thermocycler ...
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bioRxiv - Cancer Biology 2020Quote: ... An empty pENTR/D-TOPO was cut with NotI and BssHI and gel purified using QIAquick Gel Extraction Kit (Qiagen). After purification ...
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bioRxiv - Immunology 2021Quote: THP-1 human monocytes or mature primary human peripheral blood derived macrophages were transfected with control (D-001810-10-05, Dharmacon) or SP140 (L-016508-00-0005, Dharmacon) SMARTpool siRNA (100nM) using HiPerfect reagent (Qiagen) or RNAiMAX (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... genomic DNA was extracted from cells 3 d after electroporation using a QIAamp DNA Mini Kit or Micro Kit (QIAGEN) per the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2022Quote: RNA was extracted from separated shoots and roots of 14-d-old seedlings using RNeasy Plant Mini Kit (74904, QIAGEN). Quality check was carried out on a 2100 Bioanalyzer Instrument (G2939BA ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from dried and powdered 100 mg leaf or root tissue sample taken from pooled 14-d-old seedlings using RNeasy Plant Mini Kit (74904, QIAGEN). RNA was then extracted per the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... The BmAgo3 immunoprecipitated beads were divided into two and incubated in buffer D with or without 200 μg/ml RNase A (Qiagen) at 30°C for 15 min ...
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bioRxiv - Developmental Biology 2020Quote: ... All transfections were performed in D- or C-medium by using HiPerFect Transfection Reagent according to manufacturer’s instructions (301704, Qiagen, Hilden, Germany). After transfection for 24 hours ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was isolated from leaves of 21-d-old plants (as above) using the RNeasy plant mini kit (Qiagen, USA). Isolated RNA was treated with DNase (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... Up to 8 colonies were selected for each TCRα and TCRβ sequence for each of the KRASG12V/D-specific T cell clones and DNA was isolated using a QIAprep Spin Miniprep Kit (QIAGEN, 27104) and sequenced using the M13F and M13R primers ...
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bioRxiv - Cancer Biology 2023Quote: ... Deproteination of the D-2-HG quantification aliquot was achieved using 3.0 mL of Proteinase K (Qiagen, Catalog #19131, Hildren, Germany) per 100.0 mL of cell lysis solution ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from MCF10AER/vSrc cells (Control and treated with 10 nM D-Alanine for 4 h or 24 h) using RNeasy Kit (Qiagen, #74104) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from 10-d-old seedling roots (40 for each line) using the RNeasy Plant Mini Kit (Qiagen, Germany). cDNA was synthesized using TOPscriptTM RT DryMIX (dT18 ...
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bioRxiv - Biochemistry 2024Quote: ... the EcNhaA triple mutant was extracted from membranes with n-Dodecyl β-D-maltoside (DDM; Glycon) and purified by Ni-nitrilotriacetic acid (Ni-NTA; Qiagen) affinity chromatography ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from 12-d-old seedlings grown at 22°C in LD conditions at ZT13 with the RNeasy® Plant Mini Kit (QIAGEN, Germany) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Total RNAs were extracted from Wildtype MIN6 and MIN6 stably expressing shRNAs (non-specific/NS, C, D) using RNeasy kit (QIAGEN, Germantown, MD) and cDNAs were prepared using qScript cDNA SuperMix (Quantabio ...
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bioRxiv - Neuroscience 2024Quote: ... coli B21 Gold cells using 0.5 mM isopropyl thio-β-D-galactoside (IPTG) and purified on a NiNTA column (QIAGEN, ÄKTA Purifier, GE Healthcare) at 4 °C as described previously (2) ...
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bioRxiv - Genetics 2020Quote: ... 1 µl 1 mg/ml Carrier RNA (QIAGEN), 1 µl 10% SDS ...
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bioRxiv - Genomics 2020Quote: ... 1 mM EDTA and 1% freshly added Proteinase K (Qiagen)) was added to the cell suspension ...
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bioRxiv - Cell Biology 2021Quote: ... DNase 1 (Qiagen) was used to remove contaminating DNA followed by a second cleanup ...
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bioRxiv - Developmental Biology 2022Quote: ... SPACA6 concentrated to 7 mg mL−1 in Buffer B was mixed in a 1:1 volumetric ratio (0.3:0.3 mL) with JCSG+ (Qiagen), Cryos (Qiagen) ...
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bioRxiv - Biophysics 2020Quote: ... or DNAse (200 Units well−1, 1 hr, 37°C, Qiagen). DAPI was stained at 0.5 μM (5 mins ...
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bioRxiv - Immunology 2022Quote: ... Virus was inactivated by mixing 1:1 with Buffer ATL (Qiagen) prior to viral RNA extraction from NP swabs ...
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bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 μg DNA ratio using Effectene transfection reagent (Qiagen). Recombinant luciferase-expressing viruses capable of a single round of replication were released into the cell medium and were harvested 48 h later ...
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bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 µg DNA ratio using effectene transfection reagent (Qiagen). Recombinant ...
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bioRxiv - Microbiology 2023Quote: ... 5.0 x105 293T cells were co-transfected with HIV-1 Env-expressing and HIV-1 Tat-expressing plasmids at a ratio of 1:6 using Effectene (Qiagen) and incubated for 48 hours ...
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bioRxiv - Genomics 2020Quote: ... 1 ul (1U/ul) UDG and 1 ul RNaseA (100 mg/ml, Qiagen) and reactions were incubated at 37°C for 3 hrs ...
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bioRxiv - Genomics 2023Quote: ... gracilis (1 male and 1 female) using the Rneasy Mini Plant Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... purified nucleic acids were treated with 1-5 μl 1 mg/ml RNaseA (Qiagen) for 45 minutes at 37 °C to degrade RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... and 30 uL/well 1 X lysis buffer (1x Qiagen TCL, 1% beta-mercaptoethanol) was added ...
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bioRxiv - Biophysics 2022Quote: ... The YB-1 (1-180) protein fragment was purified following the manufacturer’s recommendations (Qiagen). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... supernatant was combined with Ni-NTA resin (1 mL/1 L of biomass, Qiagen) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Genomics 2023Quote: ... slides were treated with 1 mg/ml RNase A (1 mg/ml, Qiagen, 19101) in 2x SSC for at least 45 min at 37°C and then dehydrated in a 70% ...
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bioRxiv - Molecular Biology 2021Quote: ... 1× QuantiNova RT Mix (Qiagen), 1× QuantiNova SYBR Green RT-PCR Master mix ...
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bioRxiv - Microbiology 2021Quote: ... Strep 1:1000 (Qiagen, 34850).