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Citations for Qiagen :
201 - 250 of 801 citations for Chikungunya virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Cell lysate was then loaded onto Ni-NTA resin (Qiagen) and washed with binding buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Either crude tissue lysate or Ni-NTA Spin Kit (Qiagen) was used to prepare samples for Western blots ...
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bioRxiv - Microbiology 2022Quote: ... Lysate was spun through QIA Shredder columns (QIAGEN, cat. 79656) for 1 min at max speed ...
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bioRxiv - Plant Biology 2022Quote: ... with application of cell lysate on the QIAshredder columns (Qiagen) followed by the on-column Dnase I (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were further passed through a QIAshredder column (Qiagen), heated for 10 min at 95°C and separated on 4–12% Bis-Tris NuPAGE gels (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... Lysate was passed over 8 ml Ni-NTA resin (Qiagen), washed with 70 ml wash buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Bioengineering 2023Quote: ... Clarified lysates were then passed through Ni-NTA resin (QIAgen), washed with wash buffer (TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was homogenised using the Qiashredder column (Qiagen, 79654) and RNA extracted utilising the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... The cleared lysate was incubated with Ni-NTA resin (Qiagen) and washed with buffer A containing 0.5 M NaCl ...
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted from cell lysates using RNeasy (Qiagen) and an aliquot of 100 ng was reverse transcribed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleared lysates were incubated with equilibrated Ni-NTA beads (Qiagen), incubated for 2 h at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... and the clarified lysate was applied to nickel resin (Qiagen) pre-equilibrated in wash buffer (500 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... Clarified lysate was loaded onto a Strep-Tactin column (Qiagen), immobilized proteins washed with lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified lysate was incubated on charged Nickel beads (Qiagen) that have been pre-equilibrated in lysis buffer containing 1% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... the cell lysates were incubated with Ni-NTA Agarose (QIAGEN) for 2 h at 4°C and the lysate-resin slurry was washed in Lysis buffer containing 50 mM Imidazole ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were subsequently passed through a QIAshredder column (Qiagen, 79654) to eliminate debris before proceeding with nucleic acid purification ...
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bioRxiv - Bioengineering 2024Quote: ... Cleared lysate was applied to a Ni-NTA column (Qiagen) and eluted using DPBS containing 250 mM imidazole.
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bioRxiv - Biochemistry 2024Quote: ... Clarified cell lysates were purified using Ni-NTA resin (Qiagen) to produce highly purified and active enzyme ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysates were collected and transferred into QiaShredder columns (Qiagen), which were spun at 4°C for 10 minutes at 13000rpm ...
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bioRxiv - Genomics 2020Quote: The DNA of VLPs was extracted following the manufacture’s protocol for the QIAampMinElute Virus Spin Kit (QIAGEN). The resulting DNAs were quantified (Qubit ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from the sucrose-purified virus using the Qiagen DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted from mock and virus infected Vero cells using the RNeasy mini kit (Qiagen, Germany). Next ...
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bioRxiv - Plant Biology 2020Quote: DNA from virus-infected plant tissues was extracted by DNeasy Plant Mini Kit (QIAGEN, Cat. No. 69104), and 500 ng of purified DNA was subjected to bisulfite treatment using EpiTect Plus DNA Bisulfite Kit (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: The viral RNA of the egg-passaged virus was extracted using QIAamp Viral RNA Mini Kit (Qiagen). The extracted RNA was then reverse transcribed to cDNA using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA from mice serum was extracted using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... For quantification RNA was extracted from 100 μl of virus preparation using RNeasy kit (Qiagen, Manchester, UK) and analysed by reverse transcription and quantitative PCR (qPCR).
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bioRxiv - Bioengineering 2020Quote: ... Viral RNA (vRNA) was isolated from blood plasma using the Qiagen QIAmp UltraSense Virus Kit (Qiagen #53704) in accordance with manufacturer’s instructions for 0.5 mL of plasma ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from cell-free virus-containing supernatants using the viral RNA extraction mini kit (Qiagen). cDNA synthesis was conducted using Superscript IV and the uni12-FluG primer (AGCGAAAGCAGG) ...
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bioRxiv - Immunology 2021Quote: ... RNA was isolated from 200 μL sample using the QIAamp MinElute Virus spin kit (Qiagen, Frederick, MD). For the qRT-PCR assay ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The RNA from the escaped virus was isolated using a QIAamp viral RNA mini kit (Qiagen, #52904), and the full-length HA was amplified using gene-specific primers and the OneStep RT-PCR kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: Total virus-RNAs were extracted from SK.N.SH infected cells at MOI 0.5 using RNeasy minikit (Qiagen, #74104). ISRE reporter assay was performed in STING37 cells plated in 24 wells plate (2×105 cells/well ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from the concentrated virus with the QIAamp Viral RNA Mini Kit (Qiagen, 52904). The sgRNA sequences found in the genomic DNA and viral RNA samples were amplified by PCR (Agilent ...
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bioRxiv - Genomics 2022Quote: Viral RNA was extracted from 200 μl of clinical sample using QIAamp 96 Virus QIAcube HT (QIAGEN) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2022Quote: ... Viral RNA (vRNA) was isolated from blood plasma using the Qiagen QIAmp UltraSense Virus Kit (Qiagen #53704) in accordance with manufacturer’s instructions for 0.5 mL of plasma ...
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bioRxiv - Microbiology 2023Quote: ... and cleared supernatants stored at −80°C until virus titration/ spin through a QIA shredder column (Qiagen). Viral titers were expressed as TCID50 units per gram source tissue.
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bioRxiv - Microbiology 2021Quote: ... Lysates were clarified and bound to a Ni-NTA column (Qiagen) for 1h ...
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bioRxiv - Genomics 2019Quote: ... Lysates were washed twice with 650 µL of PE buffer (Qiagen) and spun through the columns at 6000 rpm for 1 minute ...
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bioRxiv - Biochemistry 2019Quote: ... the cleared lysate was loaded onto a Ni-NTA column (Qiagen) and eluted with an imidazole gradient ...
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bioRxiv - Neuroscience 2019Quote: ... RNA was purified from the lysate using RNeasy Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Centrifugation-cleared lysate was applied to Ni-NTA agarose beads (Qiagen), washed with 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Centrifugation-cleared lysate was applied to Ni-NTA agarose beads (Qiagen), washed with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: ... Centrifugation-cleared lysate was applied to Ni-NTA agarose beads (Qiagen), washed with 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biophysics 2021Quote: ... Fusion proteins were purified from clarified lysate by Ni-NTA (Qiagen) affinity chromatography ...
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CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol exportbioRxiv - Cell Biology 2021Quote: ... Clarified lysate was incubated with Ni-NTA agarose (Qiagen, Cat# 30210) for 1 h at 4 °C and after washing with buffer A + 50 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was incubated with Strep-Tactin Superflow resin (Qiagen) for 1 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The clear lysate was incubated with Ni-NTA agarose beads (Qiagen) for 2 h at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Lysates were incubated with 1 ml of Ni-NTA resin (QIAGEN) in 4°C for 1 hr ...
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bioRxiv - Microbiology 2019Quote: ... The homogenized lysate was passed through genomic DNA eliminator columns (Qiagen) and subsequently applied to RNeasy spin columns ...
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bioRxiv - Microbiology 2021Quote: ... HBV DNA was extracted from cell lysates per manufacturer’s instructions (Qiagen DNeasy 96 Blood and Tissue Kit ...
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bioRxiv - Biochemistry 2021Quote: ... the lysate was loaded onto preequilibrated Ni-NTA beads (30230, Qiagen) by gravity-flow ...