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Citations for Qiagen :
601 - 650 of 1473 citations for Calcium calmodulin Dependent 3' 5' Cyclic Nucleotide Phosphodiesterase 1C PDE1C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: Total RNA was extracted from an aliquot of 3 x 106 cells using the RNeasy Plus Kit (Qiagen). cDNA was synthesized from extracted RNA using the iScript cDNA Synthesis Kit (BioRad ...
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bioRxiv - Developmental Biology 2022Quote: ... total RNA was extracted from 2-3 embryos pooled per genotype using the RNeasy Mini Kit (QIAGEN, 74106) and QIAshredder columns (QIAGEN ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was isolated from HEK293T cells 3 days after transfections using the Gentra Puregene Cell Kit (Qiagen) and was diluted to 10 ng/μL ...
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bioRxiv - Molecular Biology 2022Quote: ... washed 3 times with PBS and had their genomic DNA extracted with the QIAamp DNA mini kit (QIAGEN). Sequencing libraries were prepared at GenomeScan (Netherlands ...
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bioRxiv - Immunology 2023Quote: ... total RNA from 2 or 3 million peripheral blood mononuclear cells (PBMCs) was extracted (RNeasy Maxi Kit, Qiagen) from each time point (2015 (112 months p.i) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was isolated from 0 or 3% CSE-treated bronchospheres using the Qiagen RNeasy Mini Kit (Qiagen 74106). The RNA yield was measured using Agilent TapeStation (4200 TapeStation System ...
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bioRxiv - Microbiology 2023Quote: ... Individual mosquitoes were homogenized for 3 minutes at a 30Hz/s frequency in a TissueLyser II grinder (Qiagen). DNA extraction of individual larvae was carried out using the QIAamp DNA Kit (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was extracted from 3-week-old plants using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... for 45 min at 4°C and the supernatant was mixed with 3 mL Ni-NTA agarose (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from 3 dpf zebrafish AB larvae (50 fish) using RNAeasy Plus Kit (Qiagen 74134). cDNA was synthesized using SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: RT-qPCR was performed in an Applied Biosystems QuantStudio 3 using the QuantiTect® Multiplex PCR Kit (QIAGEN). The primers and probes used are listed in Table S2 ...
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bioRxiv - Cell Biology 2023Quote: siRNA targeting human GPRC5A (siGPRC5A_4 against target sequence 5′-CTGGGTGTGTTGGGCATCTTT-3′, cat# SI04438021) and non-targeting control siRNA (cat# Ctrl_AllStars_1, target sequence not disclosed) were purchased from Qiagen. Human primary keratinocytes were transfected at 20 nM final siRNA concentration using Lipofectamin 2000 reagent (Life Technologies ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was then isolated for each biological replicate (3) using an RNeasy Lipid Tissue Mini kit (Qiagen). DNA contamination was removed using TURBO DNA-free (Ambion) ...
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bioRxiv - Genomics 2024Quote: ... with two 3 mm metal beads and ground into a fine powder with a TissueLyser II (Qiagen, Germany) at 30 Hz for 2 min ...
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bioRxiv - Cell Biology 2022Quote: ... single cells were sorted into PCR plates containing 5 µl Buffer RLT Plus (Qiagen) with 1% BME and immediately frozen at -80°C for G&T sequencing.
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bioRxiv - Physiology 2019Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
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bioRxiv - Physiology 2019Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
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bioRxiv - Genomics 2020Quote: ... purified nucleic acids were treated with 1-5 μl 1 mg/ml RNaseA (Qiagen) for 45 minutes at 37 °C to degrade RNA ...
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bioRxiv - Developmental Biology 2020Quote: During collection single embryos were placed for lysis in 5 ul TCL buffer (Qiagen) containing 1% (v/v ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was loaded onto a 5 ml Ni-NTA agarose bead slurry (Qiagen) equilibrated with equilibration buffer [20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Plant Biology 2021Quote: ... dry material was homogenized using 5 mm steel beads and a Tissuelyser II (Qiagen) for 1 min at 30 Hz ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were passed over 5 ml of packed Ni-NTA agarose resin (Qiagen) at 1.0 ml/min ...
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bioRxiv - Physiology 2020Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription was initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
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bioRxiv - Cell Biology 2020Quote: ... dNTPs and synthetic mRNA Spike-Ins contained in 5 μl of Vapor-Lock (Qiagen). Immediately following sorting ...
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bioRxiv - Cell Biology 2022Quote: ... frozen samples were thawed in the presence of 5 volumes of TRI-reagent (Qiagen), pipette mixed thoroughly then centrifuged at 16,000 x g for 1 min ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... grinded into a fine powder on Tissue-lyser (with 5 mm steel beads, Qiagen), centrifuged at 4°C (12,000 g ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with Trizol for 5 min at 4°C and homogenized using TissuLyserTM (Qiagen) for 5 min at 50 Hz ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Plasmids of 5 µg were transfected into 6x10[6] S2 cells using Effectene (Qiagen) and incubated for 3 days ...
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bioRxiv - Physiology 2020Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Reverse transcription was initiated with a hold at 50°C for 10 minutes (cDNA synthesis ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was applied to two 5 ml Ni-NTA Superflow cartridges (Qiagen). The columns were washed with 20 mM HEPES pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... then subsequently homogenised four times with a 5 mm diameter stainless steel bead (Qiagen) for 3 min at 50 Hz using a TissueLyser LT (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... were isolated using the “miRNeasy Mini Kit” (cell numbers ≥ 5 × 105, Qiagen, Cat# 217004). FACS-isolated FO or MZ B cells were directly sorted into the Qiazol Lysis Reagent (700 μl final volume) ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from pools of 5 females using an RNeasy Mini kit (Qiagen) by first homogenizing them using a plastic pestle in 600 µL of lysis buffer in a 1.5 mL microfuge tube ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was applied to a 5 ml nickel-nitrilotriacetic acid column (Qiagen, Valencia, CA) that had been equilibrated with buffer A at a rate of 1 ml/min ...
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bioRxiv - Pathology 2022Quote: ... with 2 x 5 mm stainless steel beads using a TissueLyser (Qiagen, Germantown, MD) for 3 minutes at 25 r/s for 2 cycles ...
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bioRxiv - Plant Biology 2022Quote: ... The suspension was applied to a column (5 ml polypropylene column, Qiagen, Hilden, Germany) with subsequent washing (5 column volumes ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from 5 million sorted bacteria using DNeasy Blood & Tissue (Qiagen, 69504) following manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Manchester, UK). Each sample was analysed in duplicate ...
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bioRxiv - Pathology 2023Quote: ... with 2 x 5 mm stainless steel beads using a TissueLyser (Qiagen, Germantown, MD) for 3 minutes at 25 r/s for 2 cycles ...
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bioRxiv - Molecular Biology 2023Quote: Approximately 5 mg of frozen liver was homogenized in 1 ml RLT buffer (Qiagen) using a BeadBeater (BioSpec ...
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bioRxiv - Microbiology 2023Quote: ... 5 ml samples of each culture were stabilized with RNA protect Cell Reagent (Qiagen) and subsequently collected by centrifugation at 5 000 x g for 10 min at 4◦C ...
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bioRxiv - Cell Biology 2023Quote: ... The control cells were replated after 5 days of AllStars negative-control siRNA (QIAGEN) treatment ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was batch bound to 5 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) resin for 90 minutes stirring in a beaker at 4ºC ...
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bioRxiv - Immunology 2023Quote: Total mRNA was isolated from 5×105 cells using a kit (RNeasy kit, QIAGEN) and reverse-transcribed using oligo(dT)20 primer (SuperscriptIII ...
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bioRxiv - Biochemistry 2024Quote: ... The solution was applied to a column of 5 ml Ni-NTA agarose (Qiagen) equilibrated in Lysis-Wash buffer and the flow-through collected ...
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bioRxiv - Cancer Biology 2024Quote: ... 1X106 cells were mixed with 5 μl of 20 μM siRNAs (GeneSolution siRNA, Qiagen) and 100 μl of Ingenio electroporation solution (Mirus ...
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bioRxiv - Molecular Biology 2023Quote: ... 40% of the protein material of each fraction was analyzed by Western blot using an anti-his antibody (Penta-His antibody, Qiagen #34660). Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody ...
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bioRxiv - Developmental Biology 2020Quote: ... we differentiated human stem cells for 3 days in mTeSR + 0.5 µM A8301 and extracted RNA with RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA from 3×107 sorted GFP+ cells was extracted using the Blood & Cell Culture DNA Maxi Kit (Qiagen). Amplification of sgRNA regions from the extracted genome and the original sgRNA plasmid library ...
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bioRxiv - Molecular Biology 2020Quote: ... while method-3 was a modified protocol of the DNeasy PowerMax Soil Kit (Cat.No. 12988-10) provided by Qiagen (based on communication exchanged with the manufacturer) ...