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Citations for Qiagen :
201 - 250 of 344 citations for Calcium Channel Voltage Dependent L Type Alpha 1C Subunit CACNA1C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Two μL of isolated RNA were used for reverse transcription (RT) and cDNA synthesis using the miRCURY LNA RT Kit (Qiagen) according to the manufacturer’s instructions adding the RNA spike-in UniSp6 as quality control for RT ...
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bioRxiv - Microbiology 2023Quote: ... in vitro in L-lactate containing minimal media and total RNA was isolated by the TRIzol method and RNeasy minikit (Qiagen). After digestion with TURBO Dnase treatment ...
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bioRxiv - Microbiology 2024Quote: Viral RNA was extracted from 100 μL of cell supernatant from passages P8 and P16 using a QIAamp Viral RNA kit on the automated QIAcube (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... was resuspended in 200 µL of PBS and DNA was isolated using the gram-positive protocol of the Dneasy Blood and tissue Kit (Qiagen). For each isolation ...
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bioRxiv - Microbiology 2024Quote: ... The pellet from the first centrifugation was resuspended in 200 µL of PBS and DNA was isolated using the cultured cells protocol of the Dneasy Blood and tissue Kit (Qiagen). The second pellet ...
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bioRxiv - Biophysics 2021Quote: ... and immunoblotting using anti-Strep antibodies (Qiagen).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... primary mouse anti-His antibody (QIAGEN, 34660), secondary goat anti-rabbit 800CW antibody (LI-COR ...
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bioRxiv - Microbiology 2022Quote: ... the mouse monoclonal Penta-His antibody (Qiagen n°34660 stock at 200 µg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Tetra·His antibodies (Qiagen, Hilden, Germany; Cat.# 34670), Anti-Mouse IgG (whole molecule) ...
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bioRxiv - Developmental Biology 2020Quote: ... 100 μl of chloroform was added and mixed vigorously and the aqueous component was isolated using MaXtract High Density tubes (Qiagen, 129046) using the manufacturers protocol ...
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bioRxiv - Neuroscience 2019Quote: ... total RNA was extracted from 200 μl conditioned medium or 100 μl foetal plasma using the miRNeasy Mini Kit and the miRNeasy Serum/Plasma Kit (Qiagen, Germany). microRNA expression levels were analysed using the nCounter Rat v1.5 miRNA Expression Assay (NanoString Technologies ...
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bioRxiv - Genetics 2021Quote: ... incubated overnight at 37°C in L-broth containing 50 ng/μL ampicillin and DNA isolated using the QIAprep Spin Miniprep kit (Qiagen 27104) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 3.5 μl of cDNA was used as template and amplified in a total volume of 40 μl with a mix of forward L-VH primers (Table S1) and reverse Cγ primer and using the HotStar® Taq DNA polymerase (Qiagen) and 50 cycles of PCR (94°C 30 s ...
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bioRxiv - Developmental Biology 2022Quote: ... The resulting 1.5 L bacterial culture was incubated at 37°C for 2h and a Maxiprep performed using Qiagen MaxiPlus kit (Qiagen #12963) to a resulting 1 mg of plasmid DNA ...
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bioRxiv - Immunology 2023Quote: ... 3.5 μl of cDNA was used as template and amplified in a total volume of 40 μl with a mix of forward L-VH primers (Table S3) and reverse Cγ primer and using the HotStar® Taq DNA polymerase (Qiagen) and 50 cycles of PCR (94°C 30 s ...
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bioRxiv - Immunology 2023Quote: ... 120 mg l− 1 sodium pyruvate and 1% penicillin–streptomycin) at 300 Hz for 2 minutes using a TissueLyser II (Qiagen, Germany).
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... Up to ∼2 million cells were resuspended in 100 μl High-TE buffer (10 mM Tris-Cl, pH 8, 10 mM EDTA, 25-100 μg/ml RNase A (QIAGEN, Cat. # 19101)) and lysed by adding 2.5 μl 20% SDS ...
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bioRxiv - Microbiology 2022Quote: 200 μl of apical wash was harvested at 48 and 72 hpi and 100 μl inactivated in 400 l AVL buffer (Qiagen, Hombrechtikon, Switzerland) for 10 min at RT then 400 μl of absolute ethanol were added to each sample ...
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bioRxiv - Neuroscience 2020Quote: ... 500 μl ethanol and 500 μl corn oil were added to 50 mg tamoxifen and mixed in a tissue lyser (Qiagen, Hilden, Germany) for 10 min at 50 Hertz ...
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bioRxiv - Microbiology 2021Quote: ... total genomic DNA was extracted from a culture grown on R2B with 200 mg/L BAM using the DNeasy UltraClean Microbial Kit (Qiagen, Hilden, Germany). Afterwards ...
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bioRxiv - Microbiology 2020Quote: ... of a 6-well plate in 200 μL ice-cold PBS + 10% Proteinase K and processed through the DNeasy Blood and Tissue Kit following manufacture protocols (QIAGEN, Hilden, Germany). gDNA was stored at −20° C for short-term storage or −80° C for long-term storage.
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bioRxiv - Microbiology 2022Quote: ... of a 6-well plate in 200 μL ice-cold PBS + 10% Proteinase K and processed through the DNeasy Blood and Tissue Kit following manufacture protocols (QIAGEN, Hilden, Germany). gDNA was stored at -20° C for short-term storage or -80° C for long-term storage.
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of cells was diluted to OD600 0.3 and 500 µL of diluted cells was added to 1 mL of RNAprotect Bacteria Reagent provided in the RNeasy Mini Kit (Qiagen; catalog #74104). Protocols provided in the “RNAprotect Bacteria Reagent Handbook 2015” were used for RNA extraction (“Protocol 1”) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA removal was performed by applying 80 μl of DNase I working solution (10 μL DNase stock + 70 μL 1x RDD buffer; RNase-Free DNase Set, Qiagen, Hilden, Germany) to the column and incubated for 15 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Microbiology 2020Quote: ... a monoclonal mouse Strep-tag antibodies (#34850, Qiagen), a monoclonal anti-actin antibody (A5441 ...
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bioRxiv - Biochemistry 2022Quote: ... Penta-His Antibody (Qiagen, 34660, IB: 1:1000) and Y188 to APP C-terminus (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... analysed by western-blotting using Penta·His Antibody (QIAGEN), of wild-type OxlT measured on the same day of experiment ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with anti-penta-his biotin antibody (Qiagen) for 1 and half hours at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies included: anti-Penta-His (QIAGEN, #34650), anti-FLAG M2 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... and HRP–conjugated anti Penta-His antibody (Qiagen). Other chemicals used in this study were of an analytical grade or higher ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies (α-His HRP conjugated (Qiagen 34460) or α -VSV-G (Millipore sigma V4888-200UG) ...
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bioRxiv - Cell Biology 2023Quote: ... were incubated with biotinylated anti-5His antibodies (Qiagen) and stored for up to 6 months with continuous rotation at 4°C in BRB80 (80 mM Pipes ...
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bioRxiv - Molecular Biology 2023Quote: ... 40% of the protein material of each fraction was analyzed by Western blot using an anti-his antibody (Penta-His antibody, Qiagen #34660). Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... Lower jaws were resuspended in 600 μl of RTL plus buffer supplemented with 1% β-mercaptoethanol (M3148-100ML, MilliporeSigma, Burlington, MA, USA) and Reagent DX (19088, Qiagen, Hilden, Germany). HH31 and HH34 lower jaws were processed in a Bead Mill 24 Homogenizer (15-340-163 ...
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bioRxiv - Developmental Biology 2020Quote: ... Lower jaws were resuspended in 600 μl of RTL plus buffer supplemented with 1% β-mercaptoethanol (M3148-100ML, MilliporeSigma, Burlington, MA, USA) and Reagent DX (19088, Qiagen, Hilden, Germany). HH31 and HH34 lower jaws were processed in a Bead Mill 24 Homogenizer (15-340-163 ...
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bioRxiv - Bioengineering 2021Quote: ... 7.5 μL of RNAse free H2O and 10 μL of 2X HotStarTaq™ Plus Master Mix (HotStarTaq™ Plus Master Mix Kit, Qiagen, USA). PCR conditions were an initial denaturation of 5 min at 95°C followed by 25 cycles of 30 s at 95°C ...
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bioRxiv - Genetics 2024Quote: ... 30 μl clarified lysate were mixed with 70 μl RNase free water and RNA isolated using the RNeasy Mini Kit (Qiagen, Germantown, MA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... the mouse anti-penta-His antibody (Qiagen; Catalog #34660) was used at a 1:2,000 dilution in 3% BSA in PBS with sodium azide ...
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bioRxiv - Biophysics 2019Quote: ... Biotinylated mouse anti-5xHis antibody was purchase from QIAGEN. Mouse anti Tubulin beta 3 antibody from BioRad (Hercules ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His is a peroxidase-conjugated antibody (Qiagen). The membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... or mouse anti-RGS-His antibody (Qiagen; cat # 34610) at 1:5000 dilution followed by goat anti-rabbit-HRP conjugate (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... mouse antibodies against the His-tag (1:1000, Qiagen), rabbit antibodies against the C-terminal region of the collagen VI α1 chain (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-penta-His antibody (34660; Lot# 136244018; Qiagen); mouse anti-Myc tag mAb ...
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bioRxiv - Microbiology 2020Quote: ... The anti-His antibody conjugated to horseradish peroxidase (Qiagen) was used at a dilution of 1:5,000 ...
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bioRxiv - Plant Biology 2022Quote: ... and NoFa-ICL was the Penta-His antibody (Qiagen) at 1:4000 in TBST ...
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bioRxiv - Immunology 2022Quote: ... then incubated with the primary antibody anti-His (Qiagen) at a concentration of 1:2500 in a solution of 3% non-fat milk in TBS-T overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... Anti-Penta-His antibody was purchased from Qiagen (Germany).