Labshake search
Citations for Qiagen :
501 - 550 of 1864 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2020Quote: ... Enzyme was inactivated (65°C, 15min) prior to PCR purification of the linearized product (Qiagen, Germany). 10μg of linearized plasmid was transformed into Pichia pastoris X-33 strain by electroporation as per the manufacturers protocol (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4°C) and supernatant was transferred to tubes containing 30µl precleared Ni-NTA agarose beads (Qiagen) in the presence of 0.05% Tween-20 and 15mM imidazole ...
-
bioRxiv - Genomics 2021Quote: Skeletal muscle previously stored at −80°C was lysed with the RLT buffer Plus buffer (Qiagen) and beta-mercaptoethanol using the TissueLyser II (Qiagen ...
-
bioRxiv - Developmental Biology 2020Quote: ... for 20 min at 37°C and purification with the RNeasy MinElute Cleanup kit (Qiagen, #74204). RNA quality was checked with the Agilent 2100 Bioanalyzer ...
-
bioRxiv - Cell Biology 2021Quote: ... snap frozen (−80°C) for proteomics analysis or dissolved in 300 ul of RNAlater reagent (Qiagen).
-
bioRxiv - Bioengineering 2021Quote: ... at 37°C for 30 min and further purified using an RNeasy Mini Kit (Qiagen, 74104) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... and incubated for 1 h at 55°C with 20 mg ml−1 proteinase K (Qiagen). Samples were treated for 10 min at 65 °C with 4 μl RNase A (100 mg ml−1 ...
-
bioRxiv - Neuroscience 2021Quote: ... Hypothalamic blocks were dissected and stored at − 80°C until RNA extraction using RNAeasy kits (Qiagen).
-
bioRxiv - Immunology 2022Quote: ... at 50 °C for 1 hour and were purified with a MinElute PCR Purification Kit (QIAGEN).
-
bioRxiv - Molecular Biology 2022Quote: ... Further these lysates were incubated overnight at 4°C with prewashed Ni-NTA beads (Qiagen, 30210).Sequential washes with wash buffer 1-4 ...
-
bioRxiv - Genetics 2022Quote: ... 3 min in 37°C shaker) and processed with DNeasy Blood and Tissue Kit (QIAGEN #69504). Two hundred ng of genomic DNA was used as input in the DamID protocol that included an improved pool of AdR primers as described (de la Cruz Ruiz et al ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... tissues were incubated overnight at 56 °C in 300 μL of ATL digestion buffer (Qiagen®) including proteinase K (Cf = 1 mg/ mL) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were stored at -80°C with the addition of RNA protect reagent (Qiagen, MD, USA). DNA was extracted using DNeasy PowerSoil kit (Qiagen ...
-
bioRxiv - Cancer Biology 2023Quote: ... Plugs were allowed to solidify at 4°C and were then incubated with Proteinase K (QIAGEN) (1 h at 50°C and then overnight at 37°C) ...
-
bioRxiv - Neuroscience 2023Quote: ... DNA was eluted two times with 50 μl of pre-heated (70°C) EB (Qiagen, 80204) through centrifugation at 8,500×g for 1 min as documented previously10 ...
-
bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 30 min followed by clean-up using an RNeasy Mini kit (Qiagen) and the modified transcripts of SINEUP-GFP RNA were recovered.
-
bioRxiv - Plant Biology 2024Quote: ... 65°C treatment with EDTA and cleaned with the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany). Depletion of ribosomal RNA (rRNA ...
-
bioRxiv - Neuroscience 2023Quote: ... Colonies were cultured for 16 hr at 37°C and DNA was prepared by maxiprep (Qiagen). The entire homology arm sequence and the whole assembled sequence was verified (Genewiz).
-
bioRxiv - Cell Biology 2023Quote: ... for 30 minutes at 37°C and purified with Minelute PCR Purification Kit (Qiagen, Cat. #28004) to produce tagmented DNA samples ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lysates were incubated at 37°C for 15 minutes following addition of 2µg RNase A (Qiagen). 1pmol ssDNA substrate 5’ DY782 ATTATTATTATTATTATTATTTCATTTATTTATTTATTTA-3’ (Eurofins ...
-
bioRxiv - Cancer Biology 2024Quote: ... and cells were washed once with PBS (37 °C) and 350 µl RNA lysis buffer (Qiagen, Buffer RLT
-
bioRxiv - Molecular Biology 2019Quote: ... Pooled libraries were diluted to 2 nM in EB buffer (Qiagen) and then denatured using the Illumina protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... cDNA synthesis was performed using RT^2 First Strand Kit (Qiagen) and run on the RT^2 First Strand Kit (Stem cell PCR array ...
-
bioRxiv - Molecular Biology 2019Quote: ... tumefaciens culture was added to 2 ml RNAprotect Bacteria Reagent (Qiagen) and RNA was isolated using RNeasy columns (Qiagen) ...
-
bioRxiv - Genomics 2021Quote: ... Pooled libraries were diluted to 2 nM in EB buffer (Qiagen) and then denatured using the Illumina protocol ...
-
bioRxiv - Microbiology 2021Quote: ... In replicates 1 and 2 a Universal extraction robot (Qiagen, Germany) and associated nucleic acid extraction (Qiagen ...
-
bioRxiv - Plant Biology 2021Quote: ... Supernatant was mixed with 2 mL of Ni-NTA agarose (Qiagen) on a rotary shaker for 1 h at 4°C ...
-
bioRxiv - Plant Biology 2020Quote: ... and finally eluted in 2 x 30 μL EB Buffer (Qiagen) at 60°C.
-
bioRxiv - Genomics 2020Quote: ... or (ii) QIAseq SARS-CoV-2 Primer Panel (Qiagen GmbH, Germany) for amplified of viral genome sequencing or (iii ...
-
bioRxiv - Biochemistry 2021Quote: ... and then stirred with 2 ml Ni-NTA Superflow resin (Qiagen) that had been pre-equilibrated with Buffer A for 10 min on ice ...
-
bioRxiv - Systems Biology 2020Quote: ... Samples were collected into 2 ml round bottom microcentrifuge tubes (Qiagen) and weighed prior to freezing ...
-
bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen) for 1 hr and washed with 50 mL wash buffer (1X PNI ...
-
bioRxiv - Immunology 2022Quote: ... 2 μg/ml TPCK-trypsin) using a Tissue Lyser II (Qiagen) with the following program ...
-
bioRxiv - Synthetic Biology 2022Quote: ... This mixture was diluted a further 2× into an Omniscript (Qiagen) RT reaction following the manufacturer’s instructions and incubated at 50°C for 20 min followed by heat inactivation at 95°C for 2 min ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Cleared lysates were incubated with 2-4ml nickel NTA beads (Qiagen) for 20-40 minutes before washing beads with 5-10 column volumes of lysis buffer ...
-
bioRxiv - Immunology 2023Quote: ... starting from step 2 of the RNeasy micro kit (Qiagen, #74004) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant was mixed with 2 ml Ni-NTA agarose (Qiagen) pre-equilibrated in lysis buffer ...
-
bioRxiv - Microbiology 2023Quote: EHEC cultures were mixed with 2 volumes of RNAprotect reagent (Qiagen), incubated at room temperature for 5 minutes and harvested by centrifugation ...
-
bioRxiv - Microbiology 2024Quote: ... The columns were put on new 2 mL collection tubes (Qiagen) and centrifuged at 15000 rpm for 1 min to dry the columns ...
-
bioRxiv - Microbiology 2024Quote: ... using a 2 ml Tube Holder Set (QIAGEN; Cat. No. 11993), and DNA extractions were eluted with C6 Elution Buffer in a final volume of 80 µl ...
-
bioRxiv - Microbiology 2024Quote: ... cleared lysates were incubated with 2 mL Ni-NTA agarose (Qiagen) on a rotator for 1 hour at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C ...
-
bioRxiv - Cell Biology 2019Quote: ... and stored at −80°C or immediately used for RNA isolation using RNAeasy Universal Mini kit (Qiagen) with on-column DNAse digestion by RNase-free DNase set (Qiagen ...
-
bioRxiv - Microbiology 2020Quote: DNA was extracted from -80°C stored stool samples using the DNeasy Powersoil HTP 96 kit (Qiagen) and an EpMotion 5075 automated pipetting system (Eppendorf) ...
-
bioRxiv - Microbiology 2020Quote: ... The soluble fraction was incubated for 1 hour at 4 °C using a Ni-NTA resin (Qiagen). The Ni-NTA resin was washed with 4 times resin volume (RV ...
-
bioRxiv - Microbiology 2021Quote: ... stored at −20° C and characterized by immnoblotting using mouse anti-2xStrep tag (Qiagen #34850, 1:1,000), and secondary goat anti-mouse IgG IRDye® 800CW (LI-COR # 926-32210 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated at 37°C for 30 min after the addition of RNase A (QIAGEN) to a final concentration of 100 µg/mL ...
-
bioRxiv - Genomics 2019Quote: ... and grown at 30°C for 18 hours before isolating library vectors using QIAprep (Qiagen, Hilden, Germany) spin miniprep kits.
-
bioRxiv - Genomics 2019Quote: ... Cell pellets were either processed immediately or kept at −80°C in RNAProtect Cell Reagent (QIAGEN # 76526).
-
bioRxiv - Pathology 2021Quote: ... at 4°C and total RNA was extracted with the RNeasy Midi kit (Qiagen, Santa Clarita, CA). Mouse Genome 430A 2.0 arrays (Affymetrix ...