Labshake search
Citations for Qiagen :
451 - 500 of 10000+ citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and 0.4 mg/mL Proteinase K (55°C for 1 hour) before being purified using the QIAquick PCR purification kit (QIAGEN). qPCR was performed on the Rotor-Gene 3000 (Corbett Life Science ...
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bioRxiv - Molecular Biology 2021Quote: ... at 55°C for 8h followed by 20 min heat inactivation at 80°C and gel purified using the QIAEX II Gel Extraction Kit (Qiagen). The gene fragments were ligated into the backbone using T4 DNA ligase (NEB ...
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bioRxiv - Genomics 2020Quote: Tissue samples were preserved in RNA-Later at −80 °C and total RNA was isolated using RNeasy mini kit (QIAGEN) as previously described 55 ...
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bioRxiv - Immunology 2022Quote: ... Kidneys were mechanically disrupted with metal beads during 3 min at 4°C and DNA was then extracted using QIAmp DNA kit (Qiagen). Leptospiral DNA was specifically targeted using primers and probes designed in the lpxA gene (L ...
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bioRxiv - Neuroscience 2022Quote: We euthanized groups of 20 to 30 zebrafish larvae by immersion in ice-cold water (below 4°C) and immediately performed RNA extraction using the RNeasy Mini Kit (Qiagen) and reverse transcription using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were snap frozen and stored at-80 °C until being processed for RNA isolation using the RNeasy RNA extraction kit (Qiagen) with DnaseI treatment using RNase-Free DNase (Qiagen) ...
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bioRxiv - Biophysics 2022Quote: ... The liquid culture was then grown for 3 hours at 37°C before the plasmid extraction using a miniprep kit (QIAGEN). Prepared DNA was sequenced by the Microbial Genome Sequencing Center (https://www.seqcenter.com/ ...
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bioRxiv - Microbiology 2022Quote: ... from 1 mL of culture were frozen at −80°C for later DNA extraction using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s procedure ...
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bioRxiv - Molecular Biology 2023Quote: ... bacteriovorus HID13, and from −80°C frozen Aquifex aeolicus pellets (purchased from the Archaeenzentrum Regensburg, Germany) using the RNEasy kit (Qiagen), including DNase I treatment ...
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bioRxiv - Genomics 2022Quote: ... The mixture was incubated at 16°C for 2 h and the resulting double-stranded DNA was purified using the MinElute PCR Purification kit (Qiagen) with 20μl EB for elution ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was mixed for 30 min at 37°C and subjected to purification using the MinElute Reaction Cleanup Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and the membrane was cut out and submerged in buffer RLT then frozen at −80°C for RNA extraction using RNeasy Kit (cat no. 74104, Qiagen), cDNA synthesis using SuperScript™ III First-Strand Synthesis System (cat no ...
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bioRxiv - Neuroscience 2023Quote: ... Sampled tissue punches were flash frozen on dry ice and store at -80 °C until RNA isolation by RNeasy Plus mini kit (Qiagen). RNA quality checking was completed with the Qubit fluorometer system (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Bacterial strains were grown overnight in broth cultures (37° C, shaking at 200 RPM) and had genomic DNA extracted using a DNeasy Blood and Tissue kit (Qiagen). Genomic DNA was sequenced using the Illumina MiSeq at the Heflin Genomics Core at UAB ...
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bioRxiv - Molecular Biology 2023Quote: ... and the chromatin incubated at 67 °C for 4 h following a purification step with the QIAquick PCR Purification kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The enzyme was inactivated at 75°C for 30 min and genomic DNA was purified using the QIAamp DNA Micro Kit (Qiagen). HLA class I (HLA-A ...
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bioRxiv - Microbiology 2023Quote: ... The homogenate was treated overnight with proteinase K (2 mg/ mL) at 56 °C and DNA was extracted using the MagAttract PowerSoil DNA EP Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Filters were snap-frozen using liquid nitrogen and stored at -80 °C until DNA extraction with the DNeasy Power Water kit (Qiagen) using the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2023Quote: ... overnight incubation at 37 °C) and DNA was extracted using the QiaAmp Blood Mini Kit (Qiagen Cat No./ID: 51304) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... primers with an annealing temperature of 60 C for 45 seconds and PCR products purified via the QIA Quick PCR Purification Kit (Qiagen). DNA concentration was measured on the Nanodrop ...
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bioRxiv - Biochemistry 2023Quote: ... Filters were incubated at 55°C for approximately 4 h and the resulting lysates were purified with the DNeasy kit (Qiagen) using a slightly modified protocol49 ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with LPS (1 μg/ml) for 6 hours at 37°C followed by RNA isolation using a RNeasy Kit (QIAGEN). Extracted RNA was converted to cDNA with an iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Following complete removal and evaporation of residual ethanol (10 mins at 30°C) DNA was extracted using the AllPrep DNA/RNA FFPE Kit (Qiagen). DNA was eluted in 40 µl Elution buffer.
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bioRxiv - Cell Biology 2023Quote: ... gDNA wipeout buffer was used to remove Genomic DNA for 2 minutes at 42°C and cDNA was synthetized with the QuantiTect Reverse Transcription Kit (Qiagen). qPCR was performed with PowerUp SYBR Green Master Mix using the StepOnePlus Real-Time PCR system ...
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bioRxiv - Microbiology 2023Quote: ... Template DNA was degraded using RQ1 DNAse for 30 min at 4°C and RNA was purified using RNAeasy mini kit (Qiagen). 4-week-old NRG mice were injected intra-hepatically using 10 µg of RNA in a maximum volume of 50 µL of PBS+RNA and one week post injection a terminal bleed was performed ...
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bioRxiv - Molecular Biology 2023Quote: ... CD4+ T cells were frozen as dry pellet at −80°C prior to DNA extraction with the DNeasy Blood & Tissue kit (Qiagen). Extracted gDNA was stored at −20°C.
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bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
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bioRxiv - Zoology 2024Quote: ... involved proteinase K digestion at 55°C using 20 µl of proteinase K and 220 µl of ATL lysis buffer (MinElute Reaction Cleanup kit, Qiagen). Prior digestion ...
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bioRxiv - Systems Biology 2024Quote: DNA for Nanopore sequencing was purified from frozen samples stored at -80 °C using a Blood & Cell Culture DNA Midi Kit (Qiagen). Approximately 100 mg of tissue sample was crushed in a BioPulveriser cooled in liquid nitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... shaking overnight at 30°C for 16hrs and the plasmid DNA is purified using a QiaPrep 96 column DNA purification kit (Qiagen). This procedure was conducted twice on different days to produce two separate replicates.
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bioRxiv - Immunology 2023Quote: ... The supernatant was aspirated out and the pellets stored at −80°C until mRNA extraction using the RNEasy Micro Kit (Qiagen). Corresponding cDNA was synthesized from the mRNA samples using the cDNA amplification kit (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... bone marrow of transplanted tumor bearing CTR and VEGF-C mice was isolated and gDNA was purified using QIAmp DNA Mini Kit (Qiagen) as described ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from five-day-old green seedlings with or without four hours of 27°C treatment of each genotype using the RNeasy Plant Kit (Qiagen). For mRNA sequencing (mRNA-seq ...
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bioRxiv - Microbiology 2023Quote: ... an unbiased amplification method was employed by amplifying the purified DNA at 30°C for 8 hours using a REPLI-g Single Cell kit (Qiagen). The amplified DNA was purified using AMPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... Digests were incubated for 2 hours at 37°C then purified using a QiaQuick PCR purification kit (Qiagen Cat#28106). Fragments of 2100 bp were size-selected using a SageELF instrument (Sage Science ...
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Phenogenomic resources immortalized in a panel of wild-derived strains of five species of house micebioRxiv - Evolutionary Biology 2023Quote: Genomic DNA was isolated from frozen (−80 °C) or alcohol-preserved muscle or spleen tissues using DNeasy Blood & Tissue Kits (Qiagen). High-quality DNA aliquots were next-generation sequenced at the Edinburgh Genomics facility using HiSeq X technology ...
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bioRxiv - Microbiology 2024Quote: ... Samples were collected and frozen at −20°C until DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen) according to the Protocol ...
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bioRxiv - Immunology 2024Quote: ... Cytosolic fractions were prepared by centrifugation at 10,000 × g for 30 min at 4°C and DNA was isolated from them using the DNeasy Blood & Tissue kit (Qiagen). The copy number of mitochondrial DNA encoding 16S RNA (RNR2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysed cell material was stored overnight at −80 °C before total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). RNA isolation and RT-qPCR was performed by the University of Rochester Genomics Research Center ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted from the supernatant (12,000 x g for 10 min at 4°C) of centrifuged tissue homogenate using an RNeasy Mini Kit (Qiagen, USA). The extracted total RNA was used for single-strand cDNA synthesis ...
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bioRxiv - Developmental Biology 2023Quote: ... These were stored at -80°C before RNA extraction using Pre cellys bead homogenisation (Bertin Technologies, France) and RNA easy Kit (Qiagen). Turbo DNA free DNase kit (Ambion ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from the cells dissociated with C&H or dispase or from fresh frozen tissues using RNeasy plus mini kit (#74134, Qiagen). 500 ng of RNA from each sample was used for cDNA synthesis using High-capacity RNA-to-cDNA kit (#4387406 ...
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bioRxiv - Genomics 2024Quote: ... were heat killed in a 95°C water bath for 20 min to produce thermolysates before DNA extraction (MagAttract kit, Qiagen) and whole-genome sequencing ...
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bioRxiv - Immunology 2024Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cý primer ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated at 37 °C for 30 min followed by immediate purification of DNA fragments with the MinElute PCR Purification Kit (Qiagen). ATAC-seq including amplification of Library and Indexing was performed as described elsewhere67 ...
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bioRxiv - Microbiology 2024Quote: ... 140 μL virus supernatant was treated with 0.25 μg RNaseA for 30 min in 37 °C to remove the extracellular RNA and continued with viral RNA extraction (QIAamp Viral RNA Kits, Qiagen) following the manufacture’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... 140 μL virus supernatant was treated with 0.25 μg RNaseA for 30 min in 37 °C and continued with viral RNA extraction (QIAamp Viral RNA Kits, Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Fecal pellets were collected and stored at −80°C until DNA extraction with DNeasy PowerLyzer PowerSoil Kit (Qiagen; 12855-100). Fecal Rg abundance was determined with Rg-specific 16S rDNA primers ...
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bioRxiv - Microbiology 2023Quote: ... The plates were then loaded onto a TissueLyser II plate shaker (Qiagen, Germantown, MD), firmly secured ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Single cells of Desulfovibrio or Methanococcus were sorted into wells of a 96-well plate containing 3 µl of PBS and Buffer D2 from Repli-G single cell kit (Qiagen) by using Influx flow cytometer (BD) ...