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Citations for Qiagen :
501 - 539 of 539 citations for BPIFB3 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with siRNA as above (Lap2: 5’-GAUGUGACAGAGCUCUCUA from Sigma; negative control: AllStars negative control from Qiagen). Total RNA was extracted with a Nucleospin RNA kit from Macherey-Nagel according to the manufacturer’s protocol from triplicates of control and Lap2-depleted samples ...
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bioRxiv - Molecular Biology 2020Quote: ... NRCMs were transfected with 25 nM final concentration of siRNAs by using HiPerfect transfection reagent according to the manufacturer’s instructions (QIAGEN).
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells suspended at 0.8 × 105 cells/ml were transfected with 50 nM siRNA using HiPerfect transfection reagent (Qiagen) according to the manufacturer’s protocol and analysed after 48 h ...
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bioRxiv - Cell Biology 2022Quote: Knockdown of ARRB2/Arrestin3 in CMV:DOR-APEX2 expressing cells was performed using “reverse” transfection in which 20 pmol siRNA (Qiagen) and 12 μL RNAimax (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: siRNA targeting ARRB2 (ARRB2-12, Cat #SI03099103) and a non-targeting control (AllStars, Cat # 1027415) was purchased from Qiagen. A pool of 4 siRNAs targeting human RME-8/DNAJC13 was purchased from Dharmacon/Horizon Discovery (Cat #L-010651-01 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 8 were transfected with a pool of cytotoxic siRNAs (“AllStars” wells, positive control, Allstars maximal death control, Qiagen). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... according to the transfection-protocol of primary smooth muscle cells with siRNA using HiPerFect Transfection Reagent according to the manufacturer’s instructions (Qiagen).
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bioRxiv - Cell Biology 2023Quote: Synthetic RNA duplexes (RBM12_8, Cat. #1027417; AllStar Negative Control, Cat. #1027281) were obtained from the validated HP GenomeWide siRNA collection (Qiagen) and transfected for 72 h using Lipofectamine-RNAiMax (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... a sequence that reveals no homology with any known mammalian gene (AllStars Negative Control siRNA, cat. no. 1027292, Qiagen), as previously described (Oehlke et al. ...
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bioRxiv - Cell Biology 2024Quote: Erc1 was genetically knocked down in B16-F1 WT cells using specific siRNA oligonucleotides targeting Rab6ip (Erc1) (Qiagen; 1027416). The cells were transfected using Lullaby transfection reagent according to the manufacturer’s instructions with a pool of 10 nM of Mus musculus Rab6ip siRNA (2.5 nM each ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with 200 nM of ON-TARGETplus SMARTpool siRNA targeting TUBB6 (Table S11) or the ON-TARGETplus Non-targeting control pool (Dharmacon) using HiPerfect transfection system (Qiagen) in RPMI ...
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bioRxiv - Cell Biology 2022Quote: ... LRRCC1-si2 (target sequence: 5’- TTA GAT GAC CAA ATT CTA CAA - 3’) and control siRNA (AllStars Negative Control) were purchased from Qiagen. siRNAs were delivered into cells using Lipofectamine RNAiMAX diluted in OptiMEM medium (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... siRNAs used were: AllStars Negative control siRNA (cat# 1027281) and si-Rab13 #8 (cat# SI02662702; target sequence: 5’-ATGGTCTTTCTTGGTATTAAA-3’) from Qiagen.
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bioRxiv - Biochemistry 2020Quote: ... 5.6 x 105 cells were seeded in 4 ml DMEM in a 6-cm culture plate and transfected with targeting or control siRNA (Table 4) (from Qiagen) to a final concentration of 20 nM for each siRNA used ...
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bioRxiv - Neuroscience 2021Quote: ... Primary OPCs growing on 6-well plates at 80% confluency were transfected with a concentration of 25 nM siRNA and 9 μl HiPerFect transfection reagent (301705, Qiagen) in the differentiation medium ...
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bioRxiv - Cell Biology 2021Quote: ... cells were seeded at 50% confluency in a 10 cm dish and transfected with 200 pmol siRNA (Qiagen, Germantown, MD) using RNAiMax Lipofectamine (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... p21 or p53 as well as a scrambled control siRNA were purchased from Dharmacon and employed at a concentration of 20nM using HiPerFect transfection reagent (Qiagen) and OptiMEM (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: BMDCs were transfected with the indicated siRNAs as described above and RNA was isolated using the RNEasy mini kit (Qiagen). cDNA was generated using SuperScript III first-strand cDNA synthesis kit (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... DRG neurons were transfected in serum free DMEM solution with predesigned fluorescently labelled siRNA directly against mouse TMEM120A (TACAN) from Qiagen-FlexiTube (cat ...
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bioRxiv - Cancer Biology 2021Quote: ES2 human clear cell carcinoma cells present (wild type) or absent (siRNA) ARID1A were collected and extracted using an RNeasy Plus Micro Kit (Qiagen). Barcoded TruSeq RNA v2 libraries (Illumina ...
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bioRxiv - Physiology 2021Quote: Total RNA was isolated from both control and high insulin-treated C2C12 myotubes before and after serum starvation or C2C12 myoblasts post siRNA transfection using the RNEasy mini kit (Qiagen). cDNA was generated by reverse transcription using qScript cDNA synthesis kit (Quanta Biosciences ...
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bioRxiv - Immunology 2021Quote: THP-1 human monocytes or mature primary human peripheral blood derived macrophages were transfected with control (D-001810-10-05, Dharmacon) or SP140 (L-016508-00-0005, Dharmacon) SMARTpool siRNA (100nM) using HiPerfect reagent (Qiagen) or RNAiMAX (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... K-R or K-Q mutant cells (post PNKP 3’-UTR siRNA transfection and GO/Bleo treatment) was performed using the QiaAmp DNA Micro kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Murine aortic SMCs in 12-well plates were transfected with 150ng siRNA (FlexiTube GeneSolution GS56458 for Foxo1, FlexiTube GeneSolution GS56484 for Foxo3, and FlexiTube GeneSolution GS54601 for Foxo4; Qiagen), respectively ...
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bioRxiv - Cancer Biology 2023Quote: RNA was harvested from melanoma cells 48 hours after siRNA or overexpression vector transfection by using RNeasy Plus mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were transfected with 50 nM double-stranded siRNA oligonucleotides (Supplementary Table 3) using HiPerFect Transfection Reagent (Qiagen, Crawley, UK).
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bioRxiv - Molecular Biology 2021Quote: ... quantitative RT-PCR, and immunoblotting. For knock-down experiments, non-specific (siNS, No. 1027310) or AHR (siAHR, SI02780148) siRNA (QIAGEN, Inc.) was transfected with LipofectamineTM RNAiMAX (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2021Quote: A total of 2 x 105 Vero E6 or HEK293ACE-2 cells were seeded 24 hours before transfection with one of the following siRNAs pools (Qiagen SMARTpool): siSTT3A (#GS3703) ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysis and RNA extraction from siRNA transfected cells were performed using the RNeasy RNA extraction kit (Qiagen Inc, Germantown, MD). 1 µg of total RNA was reverse transcribed with the High-capacity RNA-to-cDNA kit (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: After reverse transfection with different siRNAs total RNA of cells was extracted using the RNeasy Mini Kit following the manufacturer’s instructions (Qiagen, Hilden, Germany). Homogenization during extraction was performed using Qiagen QIAshredder columns ...
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bioRxiv - Cell Biology 2020Quote: ... 30 μl of 20 μM siRNA were mixed with 30 μl of HiPerFect transfection reagent (Qiagen, Cat. No: 301704, Courtaboeuf, France) or Promofectine siRNA transfection reagent (PromoKine ...
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bioRxiv - Cell Biology 2019Quote: ... MCMBP depleted cells using siRNA and MCMBP-KO (clone #1 and #2) cells was isolated using RNeasy Mini Kit (Qiagen, 74104). The cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... ONTARGETplus Non-targeting siRNA oligo#1 (NT1) 5’-TGGTTTACATGTCGACTAA-3’ (Dharmacon, D-001810-01) and FlexiTube siRNA h USP31 oligo #1 (Q1) 5’-CCGAGTTCATGAAGACCTCAA-3’ (Qiagen, SI00758422), oligo #2 (Q2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... according to the manufacturer’s recommendations in combination with the stealth siRNAs for ERK2 (FlexiTube GeneSolution GS5594 for MAPK1, Qiagen, Hilden, Germany). Non-silencing ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were transfected by lipofection at 3 days in vitro (DIV) with target siRNA or ntRNA using Effectene Transfection Reagent (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: OVCAR3 cells were transfected with different siRNAs as described above and total RNA was isolated using the Qiagen RNeasy Plus Mini kit (Qiagen, 74136) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... pooled siRNAs and control siRNAs were dispensed in duplicates at a final concentration of 5nM (20 nM for Cell Death control, Qiagen, Hilden, Germany) into white 384-well CulturPlates (Perkin Elmer ...
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bioRxiv - Cell Biology 2021Quote: U2-OS cells stably expressing MYO10-GFP were silenced for the gene of interest using a panel of siRNAs (Qiagen flexiplate, 1704159) using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Following seeding MSCs were transfected with 20nM of either pBAE:miR-29b (miR-29b) complexes or pBAE:siRNA (Scramble) complexes (Qiagen, AllStars Negative Control siRNA) or cultured in standard control medium (control ...