Labshake search
Citations for Qiagen :
1 - 50 of 76 citations for Aprepitant Beta Glucuronide Sodium Salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... The extraction buffer was modified to contain MES sodium salt instead of MES hydrate and RNase A (QIAGEN GmbH, Hilden, Germany).
-
bioRxiv - Plant Biology 2024Quote: ... mixed with 1% beta-mercaptoethanol (Qiagen) using Kimble Chase glass tissue grinders (part# KT885450-0020) ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: Genomic DNA was extracted and purified from mouse tails by a sodium hydroxide method or by proteinase K digestion followed by high-salt precipitation (Gentra/Qiagen, Valencia, CA, USA). In total ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: We extracted and purified genomic DNA from tail tissue either using a sodium hydroxide method (Truett, Heeger et al. 2000) or by proteinase K digestion followed by high-salt precipitation (Gentra/Qiagen, Valencia, CA, USA). We measured the concentration and purity of DNA using a small-volume spectrophotometer (Nanodrop ...
-
bioRxiv - Cell Biology 2021Quote: ... + 5% beta-mercaptoethanol using a bead mill (Qiagen). Samples were heated at 95° for 5 minutes to denature proteins ...
-
bioRxiv - Microbiology 2021Quote: ... Tissues were homogenized in Buffer RLT+ beta-mercaptoethanol (Qiagen). Tissue homogenate was then centrifuged through a QIAshredder homogenizer (Qiagen ...
-
bioRxiv - Genomics 2021Quote: ... and beta-mercaptoethanol using the TissueLyser II (Qiagen, Australia). DNA was extracted using the AllPrep DNA/RNA Mini Kit following the manufacturer guidelines (Qiagen ...
-
bioRxiv - Immunology 2020Quote: HPLC purified and salt-exchanged GapmeR (Exiqon/Qiagen) antisense oligonucleotides were incubated with T-cells at the indicated concentration for the indicated time ...
-
bioRxiv - Microbiology 2020Quote: ... coli 10-beta cloning strain were purified using the standard QIAprep protocol (QIAGEN), then delivered to the Pseudomonas strain via previously published electroporation83 or conjugation84 protocols ...
-
bioRxiv - Genetics 2020Quote: ... with beta-actin as a reference gene (QuantiFast SYBR Green PCR Kit; Qiagen) on a Roche LightCycler 480 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The DNA was eluted in high-salt buffer (Buffer QF) (QIAGEN). Using isopropanol precipitation ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 30 uL/well 1 X lysis buffer (1x Qiagen TCL, 1% beta-mercaptoethanol) was added ...
-
bioRxiv - Immunology 2024Quote: ... ∼1000 macrophages were sorted into 75µL of RLT buffer (Qiagen, containing 1% beta-mercaptoethanol), vortexed for 1 min and immediately frozen (−80°C) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The DNA was eluted with a high-salt buffer (Buffer QF) (QIAGEN), desalted and concentrated using isopropanol precipitation and resuspended in 10 mM Tris-HCl (pH 8.5) ...
-
bioRxiv - Genomics 2021Quote: ... Then 37.5μl of beta-mercaptoethanol (0.375% final) and 10μl RNAse A (Qiagen® 100mg/mL) were added ...
-
bioRxiv - Microbiology 2021Quote: ... then sodium bisulfite-converted (EpiTect Bisulfite kit, Qiagen). The 5’LTR or the rev regions were amplified by (semi)nested PCR (primer sequences are available upon request) ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.1% N-Lauroylsarcosine salt and 0.2% Triton-X100 and passed through Miniprep (QIAGEN) silica-fibre membrane spin columns in order to bind only the Top2cc’s and not DNA ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and treated the pools with sodium bisulfite (EpiTect, Qiagen) following a protocol for formalin-fixed paraffin-embedded samples (Gu et al ...
-
bioRxiv - Immunology 2023Quote: ... Cells were lysed in RLT (with 143mM beta-mercaptoethanol) and RNA purified using the RNeasy kit (Qiagen). 0.8-2μg of RNA were reverse transcribed using the High-Capacity cDNA reverse transcription kit and quantitative real-time PCR was carried out with the ViiA 7 Real-time PCR system (both Applied Biosystems) ...
-
bioRxiv - Immunology 2022Quote: ... RNA was extracted in RLT buffer supplemented with beta-mercaptoethanol and processed with the RNeasy Micro Kit (QIAGEN) as per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Cells were lysed in RLT buffer with Beta-mercaptoethanol and RNA was extracted using Qiagen RNEasy kit (Qiagen; #74134). Extracted RNA was then converted to cDNA using the one-step qSCRIPT cDNA solution ...
-
bioRxiv - Genomics 2024Quote: ... Homogenization buffer for RNA purification was made by adding 1:100 beta-mercaptoethanol to Buffer RLT (Qiagen, Valencia, CA) and kept on ice until use ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted with a salt-based method (Aljanabi & Martinez 1997) and an RNAse A (Qiagen) treatment was applied following the manufacturer’s recommendation ...
-
bioRxiv - Neuroscience 2024Quote: ... samples were washed four times with high salt buffer and supplemented with Buffer RLT (Qiagen #79216) before storing at -80°C ...
-
bioRxiv - Plant Biology 2022Quote: ... and sodium bisulfite treated using the Epitect Bisulfite Conversion kit (QIAGEN). The region of interest within the MeSWEET10a promoter was amplified using the primers in Extended Data Table 3 ...
-
Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... 0.005% sodium dodecyl sulfate) supplemented with 5LμL of Proteinase K (Qiagen). The cells were lysed by incubation at 55L°C for 1Lh ...
-
Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... 0.005% sodium dodecyl sulfate) supplemented with 5LμL of Proteinase K (Qiagen) after the growth medium was removed ...
-
bioRxiv - Plant Biology 2021Quote: ... Fluorescence positive cells were collected in a round-bottom polystylene tube that contains 150 - 200 μl RLT buffer with beta-mercaptoethanol (10 μl / 1 ml RLT buffer, RNeasy micro kit protocol, Qiagen). Cell sorting was performed for about 15 min and collected cells were immediately frozen on dry ice and kept in −80 °C for up to one week ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... per condition or genotype were homogenized in 2-ml Eppendorf tubes containing lysis buffer with 1% beta-mercaptoethanol using a TissueLyser LT bead mill (Qiagen) and 5-mm stainless steel beads (Qiagen #69989) ...
-
bioRxiv - Developmental Biology 2022Quote: ... from rraga mutants or wildtype siblings were sorted using BD FACSAria II directly into lysis buffer (RLT+beta-mercaptoethanol) from Qiagen RNeasy Micro Kit (74004) ...
-
bioRxiv - Physiology 2021Quote: ... Shredded bone marrow cells were frozen (−80°C) in RLT buffer (1% beta-mercaptoethanol) until RNA extraction using RNAeasy mini kit (Qiagen). RNA integrity number (RIN ...
-
bioRxiv - Immunology 2024Quote: ... semimature SP thymocytes were isolated on BD FACSAria II and BD FACSAria Fusion cell sorters directly into RLT + 1% beta-mercaptoethanol (BME) buffer (Qiagen). Libraries were generated by the Emory Integrated Genomics Core (EIGC ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK-beta cells were transiently transfected with WT or variant NaV1.2 (2 µg) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.).
-
bioRxiv - Synthetic Biology 2023Quote: ... Phoenix-Eco or Phoenix-Eco alpha-V beta-3 were transfected with LZRS-vector constructs using Effectene transfection reagent (Qiagen, 301425) as follows ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were lysed using RLT buffer mastermix (10 uL beta-mercaptoethanol : 1 mL RLT) and cells were lysed with RNAeasy micro kit (Qiagen, 74004). RNA quality and quantity was validated using a nanodrop (ThermoFisher Scientific) ...
-
bioRxiv - Molecular Biology 2024Quote: ... after which all the cells were simultaneously harvested and collected in RNA lysis buffer (RLT + beta-mercaptoethanol) (Qiagen, Venlo, The Netherlands).
-
bioRxiv - Physiology 2020Quote: ... with 0.1% (w/v) sodium dodecyl sulphate (SDS) with a Tissue Ruptor (Qiagen). Samples were centrifuged for 5 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 % (w/v) sodium deoxycholate) and once with 500 µl EB buffer (Qiagen). Beads were treated with 300 µg/ml RNase A ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted using a salt extraction protocol (Aljanabi and Martinez 1997) with a RNase A treatment (Qiagen). A total of 1438 flies were successfully genotyped at the Cf-Inv(1 ...
-
bioRxiv - Neuroscience 2024Quote: ... Unmethylated cytosines were sodium bisulphite converted to uracil using the EpiTect Sulphite Kit (Qiagen), while methylated cytosines remained unchanged ...
-
bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitates (IPs) were washed in high salt buffer and RNA from inputs and IPs extracted using the RNeasy micro kit (Qiagen).
-
bioRxiv - Bioengineering 2022Quote: ... A high salt solution in combination with isopropanol was utilized for precipitation of RNA before purification with the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... clones were isolated from a 5 ml High Salt (HS) Luria Bertani (LB) culture containing ampicillin (50 μg/ml) using the QIAprep Spin Miniprep Kit (Qiagen). Inserts in pGEM-T Easy were sequenced at Aberystwyth University’s Translational Genomics Facility and the sequence trace was analysed using FinchTV 1.4.0 software [45] ...
-
bioRxiv - Immunology 2024Quote: ... The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson, Bioscience, Salt Lake City, UT, USA) using the QuantiTect SYBR Green (Qiagen) and LightCycler® 480 system (Roche Diagnostics ...
-
bioRxiv - Biochemistry 2022Quote: ... complexes were either concentrated using a 50 kDa MWCO concentrator and further purified on a Superose 6 Increase 10/300 column (Cytiva) equilibrated in low-salt CSFxB or loaded directly onto Ni2+ agarose (Qiagen), eluted using 300 mM imidazole ...
-
bioRxiv - Genetics 2024Quote: ... cells were washed once with PBS and then lysed directly in the well using RLT buffer with beta-mercaptoethanol (Qiagen RNA-easy Mini Kit) and lysed cells were kept at −80C and processed together ...
-
bioRxiv - Cell Biology 2021Quote: ... Sodium bisulfite conversion of unmethylated cytosines in DNA was based on EpiTect Bisulfite Handbook (Qiagen). We designed primers (Table S3 ...
-
bioRxiv - Plant Biology 2021Quote: ... Total RNAs were extracted from samples collected at same times from salt-treated or control plant batches using the RNeasy plus mini kit with gDNA eliminator (Qiagen, Germany). First-strand cDNAs were synthesized from 3 μg of RNAs using SuperScript III reverse transcriptase (Invitrogen ...
-
bioRxiv - Neuroscience 2022Quote: ... Then the magnetic beads were washed in high salt buffer and RNA was extracted with the RNeasy Micro kit (Qiagen: 74004). For RNA sequencing ...
-
bioRxiv - Plant Biology 2021Quote: ... and salt treated samples (100 mM, 250 mM and 500 mM NaCl) was extracted using RNeasy Plant Mini Kit (Qiagen, Germany). RNA quality and quantity assessment ...