Labshake search
Citations for Qiagen :
101 - 150 of 290 citations for Anti SWAP 70 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... One volume of 70% ethanol was added and then transferred to a column from the RNeasy mini kit (Qiagen, 74104). RNA isolation was performed according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were combined with 400μl of 70% ethanol and loaded onto RNeasy mini columns (QIAGEN RNeasy Mini Kit, cat. 74104). RNA was extracted following the RNeasy protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
-
bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from ∼70% confluent HeLa cells grown in 6-wells plate using a RNeasy Micro Kit (Qiagen) with a DNase step according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The aqueous phase was mixed 1:1 with 70% ethanol and applied to a minikit spin column and washed with RW1 and RPE buffer (Qiagen). cDNA was synthesized using the QuantiTect Rev ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell sorting was carried out at the purity mode of sort precision using a low sheath pressure with a 70 μm nozzle and sorted cells were directly collected into ice-cold phenol and guanidine thiocyanate (QIAzol, QIAGEN) for subsequent RNA extraction ...
-
bioRxiv - Cell Biology 2023Quote: ... 600 uL) was mixed with an equal volume of 70% ethanol and loaded onto an RNA purification column (RNeasy, Qiagen). The purification was then carried out according to manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... DNA was digested on the column with 10 μL RNAse free DNase mixed with 70 μL RDD buffer (Qiagen, 79254) for 30 min at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... A volume of 1:1 of 70% ethanol was mixed with the upper phase before transfer on column (RNeasy Mini kit, Qiagen), and RNA was extracted according to manufacturer directions ...
-
bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s instructions and the resultant aqueous phase was mixed (1:1) with 70% RNA-free ethanol and added to Qiagen Rneasy mini kit columns (Qiagen) and the kit protocol was followed ...
-
bioRxiv - Cell Biology 2023Quote: ... The aqueous phase was added to an equal volume of 70% ethanol and transferred to a column from the RNeasy Mini Kit (74104, QIAGEN). The protocol for RNA extraction was carried out according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The aqueous phase was separated and added to an equal volume of 70% ethanol before using the RNeasy minikit (Qiagen) to isolate RNA following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Resulting DNA pellets were washed with 1.0 mL 70% ice-cold ethanol and re-suspended in 30 µL of EB buffer (Qiagen, Germany) after drying.
-
bioRxiv - Genomics 2024Quote: ... The aqueous phase was then mixed with an equal volume of 70% ethanol and transferred to a silica column from the RNeasy Mini kit (Qiagen). Total RNAs were then purified according to the instructions of the manufacturer ...
-
bioRxiv - Microbiology 2024Quote: ... mixed with 1 mL phenol:chloroform:isoamyl alcohol (25:24:1 at pH 8) at 70°C for 12 min and bead beating for 2 min (Tissue Lyser II, Qiagen). The mixture was centrifuged at 4°C for 3 min at maximum speed and the aqueous phase was transferred to a new reaction tube ...
-
bioRxiv - Physiology 2024Quote: ... After chloroform incubation and centrifugation per manufacture instructions RNA-containing phase was diluted with 70% ethanol 1:1 and RNA isolation was performed using RNeasy Mini Kit (Qiagen). For tissues ...
-
bioRxiv - Microbiology 2020Quote: ... the proteins were transferred to nitrocellulose membranes before being revealed with anti-Histidine monoclonal antibodies (Qiagen). Immune complexes were detected with anti-rabbit peroxidase-conjugated secondary antibodies (Promega ...
-
bioRxiv - Cancer Biology 2021Quote: ... Signal was detected using mouse anti-His antibody from Qiagen (Cat No./ID: 34660 1:500). Anti-mouse HRP was used for colorimetric development (Cat No./ID-A8924 1:1000) ...
-
bioRxiv - Cell Biology 2023Quote: ... His-tagged DmMIC10b was detected using an anti-His antibody (Qiagen N.V., Venlo, The Netherlands, 34660).
-
bioRxiv - Biophysics 2023Quote: ... the glass was additionally treated with biotinylated anti-hexahistidine monoclonal antibody (Penta-His Biotin Conjugate; Qiagen) as in Duchi et al.23 and Dulin et al.31.
-
bioRxiv - Immunology 2019Quote: ... The flow-through was mixed with an equal volume of 70% ethanol and applied to an RNeasy® Spin column (Qiagen). Contaminating gDNA was digested by on-column DNase treatment using RNase-free DNase (Qiagen ...
-
bioRxiv - Molecular Biology 2019Quote: ... An equal volume of 70% EtOH was added slowly and the homogeneous mixture was transferred to a Qiagen RNeasy spin column (RNeasy kit, QIAGEN 74104). QIAGEN protocols including a subsequent 30 min DNAse treatment ...
-
bioRxiv - Molecular Biology 2022Quote: ... The pellet was rinsed with 70% ethanol and dried at room temperature before dissolving in 100 μL of RNase-free DNase set (Qiagen 79254) and incubated at room temperature for 10 minutes to remove DNA ...
-
bioRxiv - Microbiology 2022Quote: ... The spooled DNA was washed with approximately 5 mL of 70% ethanol and dried before being suspended in 300µL of EB buffer (QIAGEN, Hilden, Germany). Purified DNA was quantified by Qubit (Thermofisher).
-
bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from cells grown in six-well plates at 70–80% confluency with an RNA extraction kit (Qiagen #74104). cDNA was synthesized using a sensiFAST cDNA synthesis kit (Meridian Bioscience #BIO-65053) ...
-
bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
-
bioRxiv - Biochemistry 2022Quote: ... Anti-His Western blots were performed using Penta-His mouse monoclonal IgG1 as primary antibody (Qiagen, #34660) at 1:2000 dilution ...
-
bioRxiv - Microbiology 2019Quote: ... and Western blot analysis was performed as previously described (30) using a primary anti-his antibody (Qiagen) and a secondary Alexa Fluor 700 goat anti-mouse antibody ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein bands were transferred to a nitrocellulose membrane and probed with primary antibodies [mouse anti-His (Qiagen) to detect CAT ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins transferred to a nitrocellulose membrane were probed with a monoclonal anti-His6 antibody (Qiagen, Germantown, Maryland). Detection of proteins via Western blotting was performed by fluorescence detection using IR-Dye®-labeled fluorescent secondary antibodies and imaged using the Odyssey CLx Imager (LICOR Biosciences ...
-
bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
-
bioRxiv - Biophysics 2021Quote: ... The capture probes contained an 11- to 12-nt target-capturing sequence with 4 LNA residues (Tm = ~70°C, estimated using Qiagen web application) for high affinity and kinetically stable capturing of nucleic acid targets on the surface ...
-
bioRxiv - Cancer Biology 2021Quote: ... DNA was then purified by mixing with beads at a 1:0.6 DNA/beads ratio followed by 3 washes with 70% ethanol and eluted with 30 μl of elution buffer (Qiagen Cat# 19086). Whole-exome sequencing was performed using the Mouse_Exome_Targets baitset from the Wellcome Sanger Institute pipeline ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... 0.5 mL of the aqueous phase was mixed with an equal volume of 70% ethanol for subsequent RNA purification by RNeasy mini kit (Qiagen, catalog # 74104) with on-column DNAse (RNase-Free DNase Set ...
-
bioRxiv - Genomics 2019Quote: ... 4°C) the aqueous phase was mixed with 700 μl 70% ethanol and applied to a Silica column (Qiagen RNAeasy Mini kit). RNA was then purified according to the manufacturers recommendations ...
-
bioRxiv - Genetics 2019Quote: ... and supernatant was mixed with 10 times its volume of “2M70” binding buffer (2 M guanidine hydrochloride and 70% isopropanol) in a 15 mL tube and passed through QIAquick silica columns (Qiagen, Hilden, Germany) using 25 mL tube extenders (Qiagen ...
-
bioRxiv - Microbiology 2021Quote: ... The aqueous phase was separated and mixed with an equal volume of 70% ethanol and immediately applied to an RNeasy Mini Spin Column (Qiagen, Valencia, CA). RNA was isolated according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: Cells seeded in 6 well plates were grown in 5% FBS to a confluency of 70% before RNA extraction using the RNEasy mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... All collected plant tissues were frozen immediately in liquid N2 and stored at −70°C until the RNA was extracted and isolated using the RNeasy mini kit (Qiagen; Hilden, Germany) and library prepped using the SQK-PCS109 kit (Oxford Nanopore Technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... the aqueous phase was mixed with one volume of 70% ethanol and passed through an RNeasy spin column (QIAGEN RNeasy Mini Kit). RNA quality was determined using the High Sensitivity RNA ScreenTape Assay (Agilent 5067-5579) ...
-
bioRxiv - Cell Biology 2024Quote: Cells were grown until 70-80% confluence and processed for total RNA extraction using RNeasy Midi Kit® (Qiagen Inc., Germantown, MD). Total RNA concentration was measured by NanoDrop® ND-1000 (NanoDrop Technologies ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... DNA removal was performed by applying 80 μl of DNase I working solution (10 μL DNase stock + 70 μL 1x RDD buffer; RNase-Free DNase Set, Qiagen, Hilden, Germany) to the column and incubated for 15 min at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: Antibodies and their conditions of use are as follows: mouse anti-His (Western blot, 1:1000; 34660; Qiagen), rabbit anti-Brl1 (Western blot ...
-
bioRxiv - Neuroscience 2021Quote: ... Approximately 250 μL of supernatant was recovered and an equal volume of 70% ethanol was then added prior to being placed in RNAeasy columns (Qiagen, Valencia, CA, USA). Columns were washed using multiple buffers and in accordance with the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and WPMY1 parental cell lines were seeded in 6-well plates and grown for 24 hours to a confluency of 70% to 90% before RNA was extracted using an RNeasy RNA extraction kit (Qiagen, Venlo, the Netherlands). Samples were prepared in triplicate ...
-
bioRxiv - Genetics 2024Quote: ... 30 μl clarified lysate were mixed with 70 μl RNase free water and RNA isolated using the RNeasy Mini Kit (Qiagen, Germantown, MA, USA) according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2022Quote: ... We added an equal volume (650 μL) of 70% ethanol to the supernatant and transferred this mixture to Qiagen RNeasy® mini columns (QIAGEN, Germantown, MD, USA). We performed downstream processes following the manufacturer’s instructions with the additional DNase I digestion step ...
-
bioRxiv - Genomics 2020Quote: ... The beads were then washed twice with 70% ethanol and resuspended in 50 μl sample elution buffer (Qiagen AE buffer with 0.1% Tween 20). After incubation at 20°C for 5 min ...
-
bioRxiv - Physiology 2019Quote: ... One volume of 70% (v/v) ethanol was added to upper aqueous phase and applied to RNeasy mini columns (Qiagen RNeasy Mini kit, Qiagen) and the remaining purification steps were carried according to manufacturer’s guidelines ...
-
bioRxiv - Genomics 2022Quote: Total RNA was isolated from day 15 fetal brain of WT and Cav1-null mice as well as from week 70 old Cav1-null mice using an AllPrep DNA/RNA Mini Kit (Qiagen, Cat No./ID: 80204) following the manufacturer’s instruction ...