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Citations for Qiagen :
1 - 50 of 169 citations for Anti CD4 TRX1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 6XHis tagged-Trx1 and Trr1 recombinant proteins were cloned in PET24 vectors and purified by column affinity onto Ni-NTA beads (Qiagen). The precursor was imported in 50 μg of the indicated yeast mitochondria in the presence of 2 mM ATP and 2.5 mM NADH at 30°C ...
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bioRxiv - Immunology 2024Quote: ... or CD8 (53-6.7) (for PbT-II CD4 T cells) prior to incubation with BioMag goat anti-rat IgG coupled magnetic beads (Qiagen) and magnetic separation ...
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bioRxiv - Immunology 2021Quote: Purified CD4+ T cells were homogenized using the Qiashredder (Qiagen, 79654) and RNA was extracted using the RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... or CD4+ T cells was extracted using the RNeasy micro kit (Qiagen) followed by cDNA synthesis using the iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... and CD95+ CD4 T cells using a RNeasy plus mini kit (QIAgen). Isolated RNA sample quality was assessed using a BioAnalyzer RNA pico assay (Agilent Technologies Inc. ...
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bioRxiv - Immunology 2021Quote: ... CD4+ cells were cross-linked and lysed according to EpiTect ChIP OneDay kit (Qiagen). After sonication to shear the chromatin ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted from sorted CD4+ T cells using the RNeasy Mini kit (Qiagen). A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... CD4+ cells were cross-linked and lysed according to EpiTect ChIP OneDay kit (Qiagen). After shearing and pre-clearing of chromatin ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from CD4+ T cells using the DNeasy blood and tissue kit (Qiagen), according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... CD4+/CD8+ T cells and Tfh from indicated organs using RNeasy Mini Kit (Qiagen Inc.). cDNA synthesis was performed using Superscript III First Strand Synthesis System (Invitrogen).
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bioRxiv - Immunology 2020Quote: RNA from CD27/CD45RA defined CD4+ T cell subsets was isolated using the RNeasy kit (Qiagen) according the manufactures instructions ...
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bioRxiv - Cell Biology 2021Quote: RNA extraction from murine CD4+ T cells was performed using the RNeasy Plus Mini Kit (Qiagen) and cDNA was generated using the QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from FACS sorted target memory CD4+ T cells with RNeasy Micro Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from FACS sorted target memory CD4+ T cells with RNeasy Micro Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Total RNA was harvested from nucleofected CD4 cells from PWH using a RNEasy plus kit (Qiagen). The quality of the RNA was assessed using an Agilent 2100 bioanalyzer with the RNA 6000 Pico Chip (Agilent Technologies ...
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bioRxiv - Bioengineering 2021Quote: Genomic DNA from human primary CD4+/CD8+ T cells was isolated using the Gentra Puregene Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... RNA was isolated from separated macrophages and CD4+ Tregs using RNeasy Mini Kit with DNAse digestion (Qiagen). Samples were frozen and shipped to Admera Health where they underwent library preparation using the Lexogen 3’ mRNA-Seq Library Prep Kit FWD HT (Lexogen ...
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was isolated from a median of 3.65 million CD4+ T cells (IQR 2.48-4.98) with the DNeasy Blood & Tissue Kit (QIAGEN). Each HIV reaction contained 825 ng of gDNA ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from in vitro activated CD4+ T cells using RNeasy Mini Kit (Qiagen Inc.) as described in manufacturer’s protocol including an on-column digestion step (RNaseFree DNase Set ...
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bioRxiv - Cancer Biology 2022Quote: CD4+ and CD8+ T cells were collected on the FACS Aria Fusion into RNAse free eppendorfs (Qiagen), transported on ice and cell pellets were resuspended and lysed in RLT buffer (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... CD3+CD8+CD45RA- and CD3+CD4+CD45RA-cell populations were bulk sorted then frozen into RLT buffer (Qiagen).
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bioRxiv - Genetics 2023Quote: ... and DNA and RNA were extracted from CD4+ T cells using the AllPrep Universal Kit (Qiagen, Hilden, Germany). Cell-associated total HIV DNA and unspliced RNA were quantified by an in-house quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Immunology 2023Quote: RNA was purified from at least 5 x 104 CD4+ T cells using the RNeasy Micro kit (Qiagen). cDNA was synthesized using the SuperScriptTMVILOTMcDNA synthesis kit (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: DNA and RNA were co-extracted from isolated CD4+ cells using the AllPrep Mini kit (Qiagen, cat #80204). RNA was used for total polyadenylated cDNA generation using dT20 primer and reverse transcription was performed with SuperScript IV First-Strand Synthesis (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from sorted CD4⁺YFP⁺ Treg cells using the RNeasy Plus Mini Kit (Qiagen, #74134), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... TCRαβ+CD4-CD45RClow/- T cells from Il34+/+ and Il34-/- rats were extracted using RNeasy Mini Kit (QIAGEN, Hilden, Germany) and protocol of 3’ DGE RNA sequencing was performed as previously described (16) ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA from 1-5 million total CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen) or phenol-chloroform and the DNA concentration was measured by Qubit High Sensitivity Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... viable in vitro differentiated CD4+ T cells were sorted into 96-well plates containing 5 μl TCL Buffer (QIAGEN) with 1% 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA (gDNA) was extracted from J-Lat cells 10.6 and HIV-1 negative CD4+ T cells with the QIAcube (Qiagen), using the QiaAmp DNA mini kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from multiples of 4 million resting CD4+ T cells according to the manufacturer’s instructions (QIAamp DNA Mini and Blood Mini Kit, QIAGEN). Amplification of NFL genomes was performed by limiting dilution ...
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bioRxiv - Immunology 2021Quote: ... and CD4 memory T cells were sorted on a FACSAria III cell sorter (Becton Dickinson) into QIAzol Lysis Reagent (QIAGEN). A total of 100,000 cells was sorted per sample ...
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bioRxiv - Microbiology 2023Quote: ... 50% confluent HEK 293T cells were transfected with 6000 ng GFP-tagged RhCMV gH-CD4 TM plasmid using Effectene Transfection Reagent Kit (Qiagen) next day ...
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bioRxiv - Immunology 2023Quote: Purified CD4+FYP+ Treg cells were stimulated with Cell Stimulation Cocktail (eBioscience) for 2 hrs and lysed in RLT buffer (Qiagen) containing 1% 2-mercaptoethanol (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from 1-2*106 sorted naïve (CD25− CD44lowCD62high) or effector (CD25− CD44highCD62low) CD4+ T-cells using RNeasy Mini Kit (Qiagen Inc.) as described in manufacturer’s protocol including an on-column digestion step (RNaseFree DNase Set ...
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bioRxiv - Pathology 2023Quote: ... RNA from CD4+ T cells (2×106 cells per condition) was extracted by using the RNeasy Plus Mini kit (QIAGEN). Extracted total RNA was quantified using the Qubit broad range RNA assay ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse brains with TRIzol or from selected CD11b or CD4-positive cells with RNeasy Plus Mini Kit (Qiagen) and reverse-transcribed ...
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bioRxiv - Cancer Biology 2023Quote: ... deep sequencing genomic DNA was isolated from PBMC or CD4+ and CD8+ sorted T cell populations following the DNeasy Blood and Tissue Kit (Qiagen) protocol ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells from pooled spleen and LN and CD69+ Trm (DAPI-/CD8a+/TCRb+/CD4-/CD69+/Ly6c-) from liver were FACS sorted directly into buffer RLT (Qiagen) supplemented with 2-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: DNA was extracted from 1×10^6 CD4 Th cells/ replicate using the Qiagen DNA mini kit according to manufacturer’s instructions (Qiagen Hilden, Germany). DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Bioengineering 2024Quote: ... was harvested from lung and spleen tissues and CD4+/CD8+ T cells using the Qiagen DNeasy Blood & Tissue kit (Qiagen, #69506) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: DNA was extracted from the cultured cells or ex vivo CD4+ T cell samples using DNeasy Blood and Tissue kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from purified CD4+ TN and TCM cells and from 24ST1NLESG cells using the RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Full-Length Individual Provirus Sequencing was performed on DNA extracted from total CD4 T cells with the DNeasy Blood & Tissue Kit (Qiagen, Cat. No. 69504), as described28 ...
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bioRxiv - Immunology 2021Quote: ... CFSEhi and CFSElo Cas9-CD45.1 Marilyn CD4+ T cells were sorted and their gDNA extracted using 10μl of lysis buffer-AL (Qiagen-DNeasy blood and tissue kit), 1μl proteinase K (Qiagen) ...
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bioRxiv - Immunology 2023Quote: iNKT cells from Acc1fl/fl and Cd4-CreAcc1fl/fl mice were sorted and total RNA was extracted using RNeasy with QIAshredders (Qiagen, Germantown, MD, USA). RNA quality and quantity were determined using an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... the membrane was incubated with freshly prepared primary antibody solution (1:1,000 dilution for anti-Alix [ab117600, Abcam], anti-Tsg101 [ab30871, Abcam], anti-Calnexin [ab22595, Abcam], anti-His [34660, Qiagen] ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-RGSHis (34650, Qiagen), anti-β-actin (AC74 ...
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bioRxiv - Biochemistry 2024Quote: ... Anti-Strep (Qiagen, 34850), Goat anti-mouse HRP-coupled secondary antibody (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... anti-penta-His (Qiagen), anti-PNPase (generated by Scrum ...
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bioRxiv - Biochemistry 2022Quote: ... an anti GAPDH monoclonal antibody (SC-47724) or an anti RGS HIS6 HRP (QIAGEN). When necessary ...