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Citations for Qiagen :
1 - 50 of 1065 citations for Adenovirus Type 5 Particles CMV GFP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... 5 μL Qiagen master mix (Qiagen Type-It Microsatellite Kit, Qiagen Inc.), 0.2 μL of each reverse primer ...
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bioRxiv - Biochemistry 2022Quote: Total RNA from cardiomyocytes was obtained on day 6 post adenovirus infection using the RNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Each reaction contained 5 μL DNA solution and PCR mixture with Taq Type-it (Qiagen®). PCR products were analyzed by capillarity electrophoresis on an ABl3130xl sequencer ...
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bioRxiv - Developmental Biology 2022Quote: ... Lentiviral particles were produced using Attractene (301005, Qiagen) in human embryonic kidney (HEK ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA was extracted from 5-day-old wild-type and Mpatg mutant thalli using the RNeasy Plant Mini Kit (Qiagen) and used as a template for reverse transcription using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2021Quote: ... PCR amplifications were carried out in 10 μl reaction volumes containing 5 μl Type-it Microsatellite PCR Kit (Qiagen, Hilden, Germany), 0.5 μM of each primer ...
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bioRxiv - Genomics 2021Quote: ... HMW gDNA was captured by magnetic particles (Qiagen MagAttract Suspension G), and then the magnetic particles with HMW gDNA was washed in wash buffer and eluted in EB Buffer (10 mM Tris-HCl ...
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bioRxiv - Genetics 2021Quote: ... and 99F8 (150 ng pU6-3-99F8-gRNA (DGRC Cat# 1549) and 150 ng pBS-99F8-5’HR-attP>>Act5C::GFP<
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bioRxiv - Microbiology 2020Quote: ... or Cignal Lenti Negative Control (CLS-NCL) lentiviral particles purchased from Qiagen. An equal volume of Bright-Glo™ Luciferase Assay System (Promega ...
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bioRxiv - Genetics 2021Quote: ... 0.4 μl H2O (Type-it Kit, QIAGEN) and 75 μg of template DNA ...
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bioRxiv - Genetics 2021Quote: ... 0.2 μl Q (Type-it Kit, QIAGEN), 0.4 μl H2O (Type-it Kit ...
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bioRxiv - Developmental Biology 2020Quote: ... EvaGreen (Type-it HRM PCR Kit, Qiagen) and the manufacturers recommended protocol for use ...
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bioRxiv - Molecular Biology 2023Quote: ... EvaGreen (Type-it HRM PCR Kit, Qiagen) and the manufacturers recommended protocol for use ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1 μl Type-it Master Mix (Qiagen), 0.17 μM of either FAM or VIC ...
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bioRxiv - Cancer Biology 2023Quote: ... siGFP (GFP-22 siRNA, Qiagen), TTLL12-specific siRNAs ...
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bioRxiv - Immunology 2019Quote: Viral RNA was isolated from virus particles with RNeasy-Kit (Qiagen, Valencia, CA). Access RT-PCR kit (Promega ...
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bioRxiv - Genetics 2019Quote: ... 1× Type-it Multiplex PCR Master Mix (Qiagen), 0.2 μM of each locus specific reverse primers ...
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bioRxiv - Microbiology 2020Quote: ... 107 viral particles/ml) was extracted using the DNeasy PowerSoil Kit (Qiagen, Germantown, MD). As the samples were liquid ...
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bioRxiv - Neuroscience 2023Quote: ... fluorescently labeled GC particles were collected in RLT (Allprep DNA/RNA Micro Kit #80284, Qiagen) supplemented with 10% b-Mercaptoethanol.
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bioRxiv - Cell Biology 2020Quote: 6xHis-tagged GFP-Fis and GFP-NusA were expressed from pCA24N and purified using Ni-NTA resin (Qiagen) in a gravity-flow column at 4°C as described (Kitagawa 2005) ...
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bioRxiv - Neuroscience 2020Quote: RNA was extracted from both GFP-positive and GFP-negative cell fractions using miRNAeasy Mini Kit (#217004, Qiagen). To test for MG enrichment of sorted samples ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was prepared from the sorted regional GFP- and GFP+ cells using the RNeasy Micro Kit (Qiagen). First-strand cDNA was synthetized from 100 ng RNA using the SuperScript III First-Strand Synthesis System (ThermoFisher ...
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bioRxiv - Physiology 2024Quote: ... The GFP+ and GFP− cells as remaining cells were either kept in QIAzol Lysis Reagent (QIAGEN, Valencia, CA), and stored at −80°C or immediately used for single cell analysis.
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bioRxiv - Evolutionary Biology 2023Quote: ... we used the Type-It Multiplex PCR Master Mix (Qiagen) 1x ...
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bioRxiv - Molecular Biology 2023Quote: ... 1X Master Mix of Type-it Microsatellite PCR Kit (Qiagen), 4 μL Q-Solution ...
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bioRxiv - Microbiology 2023Quote: ... Viral RNA was isolated from the particles using QIAamp DSP Viral RNA Mini Kit (Qiagen, USA), and treated with RNAse-free DNAse I (New England Biolabs ...
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bioRxiv - Cancer Biology 2020Quote: ... SCID GFP positive FACS-sorted cells and the mT3-2D-GFP cell line using the RNeasy Micro Kit (Qiagen). RNA concentration and quality were assessed using an Agilent BioAnalyzer ...
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bioRxiv - Neuroscience 2020Quote: ... CMV-EGFP transgenic (positive control) and 0.3k hGPR56 e1m–EGFP transgenic marmosets using AllPrep DNA/RNA Micro Kit (QIAGEN, Hilden, Germany). For sperm genomic PCR ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from single-cell clone or bulk GFP+ and GFP- cells (n = 3 with cells from three different mice in each group) using QIAzol lysis reagent (Qiagen). DNA was removed using the RNase-Free DNase Set (QIAGEN) ...
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bioRxiv - Neuroscience 2019Quote: RNA was extracted from both GFP-positive and GFP-negative cell fractions using AllPrep DNA/RNA micro Kit (#80284, Qiagen). For RT-qPCR ...
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bioRxiv - Neuroscience 2024Quote: Genomic DNA was isolated from ipsilateral and contralateral ventral midbrain hemispheres at 3 months after AAV-GFP or AAV-Cre-GFP injections using the DNeasy Blood and Tissue kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: The MS2 RNA was purified from the phage particles using QIAamp Circulating Nucleic Acid Kit (Qiagen, Germany) accordingly to the manufacturer’s protocol and stored at −80°C.
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bioRxiv - Pathology 2022Quote: DNA was extracted from the 200 μl concentrated particles solutions using the DNeasy plant mini kit (Qiagen). Two 3-mm and twenty 2-mm glass beads were added to the particles solutions together with 400 μL of lysis buffer and 4 μl RNase ...
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bioRxiv - Microbiology 2022Quote: ... viral particles were lysed and total RNA was extracted using QIAamp viral RNA mini kit (Qiagen, Switzerland) following manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... type B3 colonies using an RNeasy extraction kit (Qiagen, Hilden, Germany). The RNA extracts were enriched for bacteria by depleting coral host rRNA using a Ribo-Zero Gold Yeast kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... type B3 colonies using DNA/RNA allprep kits (Qiagen, Hilden, Germany) with β-mercaptoethanol added to the RLT+ lysis buffer following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The Qiagen Type-It Microsatellite PCR kit (Qiagen Corporation, Valenica CA) was used for PCR reactions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We performed the reactions with the Type-It PCR kit (Qiagen), according to a protocol standardized by Brown et al ...
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bioRxiv - Pathology 2023Quote: ... The PCR amplifications were performed using Qiagen Type-it kits (Qiagen) in a total volume of 11 μL ...
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bioRxiv - Neuroscience 2023Quote: ... and wild-type cells using an RNeasy Mini Kit (Qiagen, 74104). RNA was converted to cDNA using a high-capacity cDNA reverse transcription kit ...
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bioRxiv - Cell Biology 2019Quote: ... KEN-Cyclin B1-GFP/mCherry-α-tubulin and GFP-Aurora B/WT-Cyclin B1-mCherry stable cell lines were established using Effectene (Qiagen) according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... total RNA was isolated from C2C12 myoblasts transfected with GFP-empty vector or GFP-Δ(1-240)JPh1 using the RNeasy-plus mini kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 500 GFP-positive and 500 GFP-negative nuclei were sorted directly into 3uL of REPLI-g Advanced Storage buffer (Qiagen Cat#: 150365) in a PCR tube strip (Genessee Cat# ...
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bioRxiv - Genetics 2021Quote: ... We used the Type-it Microsatellite PCR Kit (Qiagen, Venlo, the Netherlands) to amplify microsatellite regions ...
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bioRxiv - Microbiology 2020Quote: ... Multiplex PCR was performed using the Type-it Microsatellite PCR kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and performed PCR reactions with the Type-it microsatellite PCR kit (Qiagen) (see Supplementary Methods for additional information on PCR procedure).
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bioRxiv - Cancer Biology 2020Quote: ... Amplification was achieved with the Type-it Multiplex PCR Master Mix (Qiagen), as previously described 39 ...
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bioRxiv - Synthetic Biology 2021Quote: ... total genomic DNA was isolated from CREAM-PUF cells transfected with CMV-SpCas9-U6-sgRNA5 using the DNeasy Blood & Tissue Kit (Qiagen, catalog number: 69504). cDNA fragments harboring both barcode and expected indel sequences were PCR amplified by using ~100 ng of the genomic DNA and primers P1 and P2 ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from GFP-empty vector-transfected and GFP-Δ(1-240) JPh1-transfected C2C12 myoblasts using the RNeasy plus mini kit (Qiagen, Hilden, Germany), based on manufacturer instructions ...
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bioRxiv - Biophysics 2021Quote: The SARS-CoV-2 RNA was isolated from virus particles with the QIAmp viral RNA mini kit (Qiagen - USA) and reversely transcribed to cDNA with the High-Capacity Reverse Transcription Kit (Thermo - USA) ...