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Citations for Qiagen :
1 - 50 of 620 citations for 9 3 bromophenyl 9 phenyl 9H fluorene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and miR-9/9*-124 + shPTBP2 using TRIzol Reagent in combination with RNeasy mini kit (Qiagen, 74104). RNA quality (RIN > 9.6 ...
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bioRxiv - Microbiology 2020Quote: ... and a plasmid expressing VSV-G or HIVKB9 envelope glycoproteins were cotransfected at the mass ratio of 9:1 (9 Hi.fate / 1 Env) using Effectene (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: We extracted RNA from 35 pituitaries and 35 gonads (17 Independents, 9 Satellites, 9 Faeders) using the RNeasy Minikit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... and chestnut oak with FUSE (n = 9) and conventional extraction methods (n = 9) using a lysis buffer and purified using silica columns (Qiagen DNeasy Plant Kit Qiagen Inc ...
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bioRxiv - Microbiology 2022Quote: ... coli strains containing expression plasmid pQE-9 (Qiagen), pQE9-ftsA ...
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bioRxiv - Microbiology 2023Quote: ... and the CLC Genomics Workbench 9 software (Qiagen) for analysis ...
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bioRxiv - Immunology 2024Quote: ... Samples were diluted 9:1 (elution buffer (Qiagen):cDNA ...
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bioRxiv - Microbiology 2020Quote: ... and 9 dpi with a RNeasy Mini Kit (Qiagen) with DNase treatment ...
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bioRxiv - Microbiology 2021Quote: ... 6 and 9 employing the RNeasy plant mini kit (Qiagen). RNA was quantified using Nanodrop (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... and 9-10 mL of Buffer BB (1/3 of the lysate volume, From QIAGEN Plasmid Plus Midi Kit) was added ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Combined digests were extracted with 9 × volumes of PB buffer (QIAGEN) and DNA was purified with MinElute columns on a QIAvac 24 Plus vacuum manifold system (QIAGEN).
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bioRxiv - Cell Biology 2021Quote: ... The plasmid DNA was extracted from 3-9 positive colonies where possible after blue-white screening using a QIAprep® Spin Miniprep Kit (Qiagen) and sequenced using the standard T7 primer at Eurofins Genomics ...
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bioRxiv - Genetics 2022Quote: ... and non-inoculated G305-3M leaf segments collected along 10 different time points (0, 3, 6, 9, 12, 16, 24, 36, 48, 72 hpi) using the RNeasy Plant Mini Kit (Qiagen, Germany). The cDNA was synthesized from total RNA using a qScript™ cDNA Synthesis Kit (Quantabio ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 M Tris/Bicine pH 8.5 with 9 µL of JCSG+ (Qiagen) condition #29 containing 0.8 M sodium phosphate monobasic monohydrate ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Digests were combined and DNA was extracted with 9 × volumes of PB buffer (QIAGEN) or a 3:2 mixture of QG buffer (QIAGEN ...
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bioRxiv - Neuroscience 2022Quote: ... and 15μL of 100ng/mL d3-CBD (Qiagen TissueLyser II (30 hz, 9 min)) ...
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bioRxiv - Molecular Biology 2023Quote: ... After purification of AtH2B.9 using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAGEN), the His6-tag portion was removed by TEV protease (30 µg/mg of His6-H2B.9) ...
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bioRxiv - Genomics 2021Quote: 72 µl Proteinase K digestion solution (63 uL Qiagen PKD buffer, 9 uL Proteinase K) was injected into the flow cell and incubated at 55°C for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNAs from the pool and var2-9 were extracted with the DNeasy Plant kit (Qiagen). One μg of genomic DNA was used for library construction with the Truseq DNA PCR-free kit (Illumina ...
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bioRxiv - Genomics 2024Quote: Total DNA was extracted from 6-9 ml EDTA-blood using the FlexiGene DNA Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... organs from adult mice (9-13 weeks old) were harvested and homogenized in Qiazol lysis reagent (Qiagen) and total RNA was isolated by phenol–chloroform extraction according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... organs from adult mice (9-13 weeks old) were harvested and homogenized in Qiazol lysis reagent (Qiagen) and total RNA was isolated by phenol–chloroform extraction according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Extraction was performed on the 9 samples using the DNeasy® PowerSoil® Pro Kit (QIAGEN, Germany) according to the manufacturer’s protocol.
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bioRxiv - Evolutionary Biology 2024Quote: ... The remaining procedure was identical to that provided in the DNeasyPlant Mini protocol (steps 9-19, Qiagen). After DNA quality and quantity filtering ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was isolated from 9 independent biological replicates for each genotype using the RNeasy Plus Kit (Qiagen #74136) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Whole seedlings were harvested after 9 d and total RNA was extracted with RNeasy Plant Mini Kit (QIAGEN) following the manufacturer’s protocols ...
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bioRxiv - Genomics 2021Quote: RNA was extracted from fresh-frozen brain tissue (Brodmann area 9) using the RNeasy lipid tissue mini kit (QIAGEN); 20 mg of tissue was homogenised in 1 mL QIAzol lysis reagent using a 1 mL syringe successively with 23G and 25G needles ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from dissected brains (n = 9 brains per experimental cohort) by RNeasy Mini Kit (Qiagen, 74104), with on-column removal of genomic DNA (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... the infected host cells were washed with PBS and RNA was harvested using the RNeasy Mini Kit 9(Qiagen). Total isolated RNA was processed for next generation sequencing as previously described (Sokol et al. ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted from three biological replicates with 9 ears per replicate using Plant RNeasy Mini Kit (Qiagen). For the vegetative analysis ...
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bioRxiv - Immunology 2022Quote: ... the DNA was extracted from 100 mg mouse fecal samples (9-week-old, 2 weeks after final oral gavage treatment) using QIAamp PowerFecal Pro DNA kit (Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: ... MDA was done directly on cells (Supplementary figure 9) using the REPLI-g Single Cell Kit from Qiagen (Cat #150343) following kit-specific protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Primary OPCs growing on 6-well plates at 80% confluency were transfected with a concentration of 25 nM siRNA and 9 μl HiPerFect transfection reagent (301705, Qiagen) in the differentiation medium ...
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bioRxiv - Plant Biology 2020Quote: ... 65 cabbage and 9 broccoli accessions) were collected and used to extract DNA using the DNeasy Plant Mini Kit (Qiagen). Pair-end libraries with insert sizes of ~400 bp were constructed using the NEBNext Ultra DNA Library Prep Kit according to manufacturer’s instructions and sequenced on an Illumina HiSeq 2500 platform with the paired-end 2×150 bp (117 accessions ...
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bioRxiv - Neuroscience 2020Quote: Mitochondrial and nuclear DNA was isolated from pupae 9 days after egg hatching using the DNeasy Blood & Tissue kit (Qiagen). A total of 50 ng genomic DNA was used as a template to perform qPCR using iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... The plasmid coding for FLP recombinase and the mCherry reporter or 47bp-insertion TLR reporter plasmid were transfected into the cells at a 9:1 ratio using polyfect (QIAGEN) according to the manufacturer’s protocol (900ng mCherry reporter or 47bp-insertion TLR reporter plasmid and 100ng FLP recombinase plasmid to make 1 μg plasmid in total) ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was collected from multiple stages of zebrafish development (30%-epiboly, 9 somite, Prim-5, High-pec and Larvae) using the miRNeasy kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... all infected host cells were washed with PBS and total RNA was harvested using the RNeasy Mini Kit 9 (Qiagen). This RNA was then processed for next generation sequencing as previously described (Sokol et al. ...
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bioRxiv - Neuroscience 2023Quote: RNA was isolated from hippocampal tissues of 4- and 9-month-old WT and Tau mice using RNeasy Mini kit from Qiagen with DNase digestion ...
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bioRxiv - Plant Biology 2023Quote: ... Multiple sequence alignment followed by phylogenetic tree construction of precursor miR528 was performed using the alignment tool of CLC genomics (version: 9, Qiagen). The evolutionary tree was constructed using the maximum likelihood method and the Tamura-Nel model (Tamura et al. ...
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bioRxiv - Plant Biology 2023Quote: Methylation levels in different tissues under control and drought conditions were determined by analyzing the whole genome bisulfite datasets using the bisulfite sequencing plugin of the CLC Genomics Workbench (version: 9, Qiagen) following default parameters ...
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bioRxiv - Physiology 2024Quote: ... A fraction (10%) of the total lysate volume was mixed with 9 volumes of QIAZOL (Cat. # 79306; QIAGEN, Hilden, Germany) and frozen at -80°C ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was extracted from EYFP-positive VCS cardiomyocytes obtained by sorting from 9 weeks old control and VCS-specific Tbx3:Tbx5-deficient mice using RNeasy Mini Kit (Qiagen), followed by DNase treatment according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Electroporated bacteria was split across six tubes and each recovered in 2 mL of SOC for 1 h at 37 °C then added to 500 mL of LB with 100 μg/mL of carbenicillin and grown for 9 h at 37 °C prior to plasmid purification (Qiagen, 12991). The plasmid prep was then normalized to 1 μg/μL to generate the final mpra:minP:gfp library used for transfection delivery.
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bioRxiv - Genomics 2022Quote: ... equal volumes of the clones were combined together and the pool expanded in 20 mL of LB with 100 μg/mL carbenicillin for 9 hr followed by plasmid purification (Qiagen, 12943). Approximately 250 colonies in total were harvested.
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bioRxiv - Biochemistry 2021Quote: Purified CrFBA3 was tested for crystallization at two concentration of 9 mg/ml and 4.5 mg/ml on commercial sparse-screening conditions (Qiagen, Hilden Germany) based on the work of Jancarik and Kim (Jancarik ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from 9 AAA and 10 PAD patients’ serum using the miRNeasy Serum/Plasma Advanced Kit (Qiagen, Hilden, Germany), following in principle the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples were initially preserved with 1 mL of activated sludge (i.e., suspended phase) in 9 mL of LifeGuard Soil Preservation (Qiagen, Hilden Germany). This solution was thawed and centrifuged (16,000 g for 1-minute ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted from 7–9-week-old glial spheres (FMR1+/y and FMR1-/y) using the RNeasy micro kit from Qiagen (74004). RNA was converted to cDNA using RevertAid First Strand cDNA Synthesis Kit (K1621 Thermofisher) ...
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bioRxiv - Physiology 2020Quote: ... existing media was removed and the resulting myotubes received either 750 µl of fresh DM alone or 750 µl DM containing 9 µl HiPerFectTransfection™ (Qiagen, UK) and 20 nmol Flexitube GeneSolutions™ (siUBR5 ...