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Citations for Qiagen :
601 - 650 of 2343 citations for 6 Chloro 5 fluoro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... or IFNγ (10ng/mL) for 6 hours prior to harvesting and RNA extraction using RNeasy Mini Kit (Qiagen 74106). Next ...
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bioRxiv - Molecular Biology 2019Quote: 1×106 MIA PaCa-2 or HAP1 cells were seeded per 6 well plate and co-transfected with 0.5 µg pCas-Guide plasmid using Effectene transfection reagent (Qiagen). 24 hrs after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were then seeded in 6-well dishes (6x105 cells/well) and reverse transfected using Polyfect (Qiagen, 301105) following the manufacturers protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The expressed recombinant protein was solubilized in a denaturing buffer (6 M HCl-Guanidine, 20 mM Tris-HCl[pH 7.5] and purified by Ni-NTA (30210, QIAGEN) under denaturing conditions ...
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bioRxiv - Cell Biology 2022Quote: Cells were grown in 6 well plates and total mRNA was isolated using the RNeasy Kit (Qiagen, 74-104) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... after adding 1.0×10^6 copies/µL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... solubilized in a denaturing buffer (6 M HCl-Guanidine, 20 mM Tris-HCl pH 7.5) and purified by Ni-NTA (QIAGEN) under denaturing conditions ...
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bioRxiv - Genomics 2023Quote: ... Plasmid transfections were performed in six-well tissue-culture treated dishes at 1.8 × 10^6 cells/ml using Effectene (301427; QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... then diluted to 2 nM using elution buffer (Qiagen) with 0.1% Tween20 (Sigma) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and ground for 2 min using a TissueLyser (Qiagen). DNA extraction was performed using a 96-well column based kit ...
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bioRxiv - Genomics 2019Quote: ... and BS (2) EpiTect Bisulfite Kit (QIAGEN, Hilden, Germany) respectively according to the manufacturers’ recommendations ...
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bioRxiv - Biophysics 2020Quote: ... 2 mM PMSF) on a Ni-NTA resin (Qiagen). Bound proteins were washed (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... then diluted to 2 nM using elution buffer (Qiagen) containing 0.1% Tween20 (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... the 2 proteases of proteinase K (Qiagen, Hilden, Germany) and dispaseII (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... 2 μl of 100 mg/mL RNase A (Qiagen) was added and the tubes were incubated at 37°C for 15 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... 2 volumes of RNAprotect Bacteria Reagent (Qiagen, Hilden, Germany) are added to one volume of bacterial culture ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µl Qiagen CL buffer (10x; Qiagen, Hilden, Germany), 0.4 µl MgCl2 (25 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5 µg/mL anti-Penta-His-Alexa Fluor 488 (Qiagen), which recognized the His-tag on PXDN-con4 ...
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bioRxiv - Genomics 2020Quote: ... 5 mL nuclease-free water) instead of customary Elution Buffer (Qiagen) (see Supplementary Material Methods) ...
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bioRxiv - Neuroscience 2020Quote: ... 0.08 μl of 5 U/μl HotStarTaq DNA polymerase (Qiagen 203203) and 15.1 μl or 14.5 μl milliQ water ...
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bioRxiv - Neuroscience 2022Quote: ... STHdh cells were transfected with FlexiTube siRNA (5 nmol) from Qiagen (Mm_Csnk2a2 ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was incubated with 5 mL Ni-NTA resin (Qiagen) for 2 h ...
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bioRxiv - Cancer Biology 2019Quote: ... with the addition of a 5 mm stainless steel bead (Qiagen) and ice-cold 50% methanol/20 μM L-norvaline (450 μl) ...
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bioRxiv - Biochemistry 2020Quote: ... and bead-bashed for 5 min at RT (Qiagen TissueLyser II). Lipid extracts were prepared using a modified version of the Bligh and Dyer lipid extraction [70] ...
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bioRxiv - Molecular Biology 2019Quote: ... and 4-5 hours later DNA was transfected using Attractene (Qiagen). Briefly ...
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bioRxiv - Microbiology 2019Quote: His Tag-Alexa 647 (5 ug/mL) (Qiagen, catalog no. 35370)
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bioRxiv - Microbiology 2019Quote: ... 0.125 μl Taq DNA Polymerase (5 units/L, Qiagen, Hilden, Germany) and 1 μl of DNA template ...
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bioRxiv - Immunology 2020Quote: ... which contained 5 μl of sort buffer consisting of (1X Qiagen One-step RT PCR Buffer (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed using 5-10 ml RBC lysis buffer (Qiagen) and stained for flow cytometry analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysate was loaded on 5 mL Ni-NTA column (Qiagen) equilibrated with buffer C ...
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bioRxiv - Molecular Biology 2023Quote: ... DMSO or 5-aza-dC-treated cells (51304, Qiagen, DUS, GER). 200 ng DNA was dropped onto a positively charged Nylon membrane (Amersham Biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM BME) and purified through Ni-NTA agarose beads (Qiagen) (Denaturing Elution Buffer ...
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bioRxiv - Bioengineering 2023Quote: ... on a 5-plex QIAcuity One digital PCR instrument (Qiagen, 911021). The thermal cycling conditions were implemented using the following program ...
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bioRxiv - Microbiology 2023Quote: ... the tissue was homogenized with stainless steel beads (5 mm; Qiagen) in a TissueLyser II (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 mM BME) and purified through Ni-NTA agarose beads (Qiagen) (Denaturing Elution Buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mL Ni-NTA Strep-tactin superflow (Qiagen, Germantown, MD, USA) columns were used ...
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bioRxiv - Microbiology 2024Quote: ... The incubated resin was loaded into 5 ml polypropylene column (Qiagen) subsequently multiple washes were applied using a wash buffer pH 8.0 (50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2020Quote: Whole genome amplifications were performed on DNA extracts at dilution 10 times through a multiple displacement amplification (MDA) step of 6 to 7 hours using the REPLI-g Midi Kit (QIAGEN) and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... we extracted total RNA from CD4+/CD8+/CD3+ T cells (from spleen or bone marrow) of Foxp3-GFP or C57BL/6 mice using RNeasy Micro Kit (Qiagen) and cleaned from excess DNA with DNAse 1 enzyme (Promega) ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from blood (n=6 for each housing group) using the RNeasy Protect Animal Blood Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... or trl RNA was was calculated using the 2-ΔΔCt method56 using RNA extracted from 500ul of cells following the 6 day incubation using an RNeasy Plus RNA extraction kit (Qiagen). Gene targets were amplified from cDNA using previously validated primers for clamp ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Cancer Biology 2019Quote: ... was isolated from human PanNET specimens or cells grown on 6-cm or 10-cm plates using RNeasy mini kit (Qiagen) containing gDNA eliminator spin columns ...
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bioRxiv - Genomics 2019Quote: ... Genomic DNA was extracted from 2 batches of 50 adult females from each condition (F0 GuyR, F0LD80, F2LD25 and F2LD75, see Fig. 6) using the PureGene kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... T cells (from pooled spleen and pLNs in Figure 4 or from mLN in Supplementary Figure 6) from Foxp3Cre WT mice and Tet2/3fl/flFoxp3Cre DKO mice (14-weeks old) using RNeasy plus mini kit (Qiagen). RNA-sequencing libraries were prepared using Truseq stranded mRNA kit (Illumina ...
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bioRxiv - Cell Biology 2019Quote: ... 1.2×106 cells were plated into each well of a 6-well plate and transfected with Sns and Eff-1 constructs (200ng each) using Effectene (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 5.6 x 105 cells were seeded in 4 ml DMEM in a 6-cm culture plate and transfected with targeting or control siRNA (Table 4) (from Qiagen) to a final concentration of 20 nM for each siRNA used ...
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bioRxiv - Neuroscience 2021Quote: ... SH-SY5Y cells were treated either with 100 μM cycloheximide (CHX) or DMSO for 6 hours (41) before harvesting the RNA through RNeasy Minikit (Qiagen). Reverse transcription was performed using RevertAid cDNA synthesis kit (Thermo) ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted by quantitative RT-PCR from Col-0 and DWF4-OE seedlings 6 days post germination with the RNeasy Mini Kit (Qiagen). For DWF-OE ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from cells (6-well dishes) or liver pieces (∼10-15mg) using the RNeasy Plus Mini kit (Qiagen). For livers ...