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Citations for Qiagen :
351 - 400 of 781 citations for 6 CHLORO CHROMAN 3 YLAMINE HYDROCHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was extracted from both hippocampi of 6 P12 C57BL/6J male mice using the miRNeasy mini kit (Qiagen). The sequencing pipeline is based on the Illumina’s Small RNA Digital Gene Expression v1.5 protocol with minor modifications to include indexing and multiplexing of RNA samples ...
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bioRxiv - Neuroscience 2022Quote: ... iPS-NES cells were plated in 6-well plates and lysates were collected in RNAprotect® Cell Reagent (QIAGEN, #76526) to stabilize the RNA ...
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bioRxiv - Immunology 2022Quote: ... diluted in fresh culture media or left untreated (n = 4) for 6 h prior to harvesting RNA in RLT buffer (Qiagen) with added β-ME ...
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bioRxiv - Biochemistry 2022Quote: ... Genomic DNA for sequencing was prepared from 25 L3 salivary gland pairs or 10 mg embryos (0-6 h AED) using DNeasy Blood and Tissue kit (Qiagen). Each sample was prepared in triplicate ...
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bioRxiv - Biochemistry 2022Quote: 3×106 cells from the day 8 of CD34+ HSPC and day 6 of HUDEP-2 erythroid differentiation were used for total RNA using RNeasy Mini Kit (Qiagen). For reverse transcription using Primescript RT reagent kit (Takara Bio Inc.) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from ∼70% confluent HeLa cells grown in 6-wells plate using a RNeasy Micro Kit (Qiagen) with a DNase step according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: RNA was isolated from 6-day old Arabidopsis plants using the QIAGEN RNeasy Mini Kit (ID: 74104, QIAGEN, Hilden, Germany). RNA quality was determined using a Nanodrop 2000/2000c spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 1× PBS and total DNA was extracted at 6 hpi with DNeasy Blood/Tissue DNA mini kit (Qiagen) and quantitated by a nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Expressed proteins were harvested by centrifugation 6 days post transfection and immediately purified from the supernatant on a Ni-NTA Superflow column (#30430, Qiagen). Eluted protein fraction was buffer exchanged into PBS pH 7.4 using a HiPrep 26/10 Desalting column (#GE17-5087-01 ...
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bioRxiv - Microbiology 2023Quote: ... cells were grown in TSB for 6 hr and total bacterial RNA was isolated using the RNeasy mini kit (Qiagen) with on-column DNA digestion according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA was harvested at 6 and 24 h post IFN-treatment (three independent biological triplicates) and isolated using the RNeasy Mini Kit (Qiagen). RNA-Seq was performed by Novogene using a PE150 sequencing strategy on the Illumina NovaSeq 6000 platform ...
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bioRxiv - Biochemistry 2022Quote: ... complexes were either concentrated using a 50 kDa MWCO concentrator and further purified on a Superose 6 Increase 10/300 column (Cytiva) equilibrated in low-salt CSFxB or loaded directly onto Ni2+ agarose (Qiagen), eluted using 300 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells from single wells of a 6-well plate were pelleted and total RNA was extracted using the RNeasy MiniKit (QIAGEN), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Swabs were incubated at 37 degrees Celsius for 30 minutes then bead beaten for 6 minutes at 30Hz in a TissueLyser II (Qiagen). The rest of the protocol was followed increasing the volumes to account for the additional lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with LPS (1 μg/ml) for 6 hours at 37°C followed by RNA isolation using a RNeasy Kit (QIAGEN). Extracted RNA was converted to cDNA with an iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from FACS-sorted L-GMPs from BL/6 and Il21-/- AML mice using the RNeasy Micro Kit (Qiagen). RNA purity was checked using the NanoPhotometer® spectrophotometer (Implen) ...
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bioRxiv - Systems Biology 2023Quote: ... DAPT was washed out and cells were allowed to signal for 6 hours before cells were harvested for RNA extraction using QIAshredder columns (Qiagen) and the RNeasy Mini Kit per the manufacturer’s instructions (see next section for further details of the plated ligand assay) ...
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bioRxiv - Microbiology 2023Quote: ... 5.0 x105 293T cells were co-transfected with HIV-1 Env-expressing and HIV-1 Tat-expressing plasmids at a ratio of 1:6 using Effectene (Qiagen) and incubated for 48 hours ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted total RNA from liver and BAT from each sample (n = ∼6 per genotype/sex/treatment/tissue) using the RNeasy PowerLyzer Kit (QIAGEN). We generated Illumina cDNA libraries from 1 μg of purified RNA using KAPA Stranded mRNA-Seq Kit (Illumina) ...
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bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
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bioRxiv - Cell Biology 2023Quote: The entire RNA was isolated from a single well of a 6-well plate using the RNeasy Plus Micro Kit (QIAGEN), following the guidelines provided by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: Monolayer of 1.0 × 106 cells grown in 6 well plate was lysed in 500 μl/well QIAzol Lysis Reagent (Qiagen #79306) and RNA extraction was performed with QIAzol miRNeasy Mini Kit (Qiagen #217004 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins harvested after 6 days post transfection were purified from the supernatant on a Ni-NTA Superflow column (#30430, Qiagen). Immediately after the elution ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin was treated with 1% SDS and 0.1 M NaHCO3 for 6 h at 65°C to reverse the cross-links and DNA was purified using columns (Qiagen) followed by ChIP-seq library preparation using ThruPLEX kit (Rubicon ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted from paired ovaries (N=6 per condition) using a miRNeasy micro extraction kit per the manufacturer’s instructions (Qiagen, 217084). RNA concentration and quality were assessed using the RNA Total RNA Nano Assay (Agilent Technologies) ...
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bioRxiv - Immunology 2021Quote: Total RNA was isolated from 3×106 neutrophils by using the RNeasy Mini Kit (Qiagen). TaqMan real-time quantitative RT-PCR was performed utilizing the Applied Biosystems StepOne Plus cycler (Applied Biosystems ...
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bioRxiv - Systems Biology 2020Quote: ... with 3 mm beads then extracted according to the protocol using RNeasy Mini Kit (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... 3 × 105 cells were harvested and total RNA was extracted using the RNeasy kit (Qiagen) employing on-column DNase treatment ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... crassa strain 87-3 (bd+, mat a) using the Gentra Puregene Tissue kit (Qiagen, 158622) and following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and roots of the cultivar Hoko-3 using RNeasy Plant Mini kit (Qiagen, Hilden, Germany). Library preparation was performed using NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... Typhimurium strain were (n = 3) were extracted using the Rneasy kit (Qiagen Sciences, Maryland, USA), after an overnight culture in CBD tinted LB broth (for CBD-resistant strain ...
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bioRxiv - Plant Biology 2023Quote: ... Two glass beads were added before shaking for 3 minutes using a Tissue Lyser (QIAGEN) at maximum speed ...
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bioRxiv - Cancer Biology 2023Quote: ... Equivalent cell numbers (∼3 × 106) across all samples were harvested in QIAzol lysis reagent (Qiagen). Illumina gene expression raw data were transformed using variance- stabilizing transformation (VST ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was ground using a tissue lyser (Qiagen, 3 min at 300 s-1) in 200 µL 500 mM Tris pH 9 by adding a metal ball (diameter 5 mm) ...
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bioRxiv - Genomics 2023Quote: ... 3) cleanup of final libraries with QIAgen MinElute PCR purification kit (QIAgen, Cat. No 28004), followed by a second cleanup with 2X SPRI ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated for 3 minutes at 56°C in a deparaffinization solution (Qiagen, 19093). A step of digestion with Proteinase K was performed followed by a treatment with DNAses ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was then incubated with 3 ml of Ni-NTA Superflow agarose beads (QIAGEN), which were preequilibrated with bacterial lysis buffer ...
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bioRxiv - Immunology 2024Quote: ... Total RNA (3 independent samples per group) was isolated (QIAGEN miRNeasy mini extraction kit (QIAGEN) and cDNA was synthesized (High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems)) ...
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bioRxiv - Immunology 2024Quote: ... Total RNA (3 independent samples per group) was isolated (QIAGEN miRNeasy mini extraction kit (QIAGEN) and cDNA was synthesized (High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems)) ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was briefly incubated (approximately 5 min) with 3 ml Ni-NTA Agarose (QIAGEN) before flow-through was collected ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from whole brain of 6 dpf larval zebrafish using RNeasy micro plus RNA extraction kit (Qiagen, Germany). Total RNA (2ng/µl ...
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bioRxiv - Neuroscience 2020Quote: ... RAGE-/- and SOD1G93A x RAGE-/- mice (n = 5 - 6) using RNeasy Lipid Tissue extraction kit according to manufacturer’s instructions (QIAGEN, CA, USA). The total RNA was purified from genomic DNA contamination using Turbo DNase treatment (Ambion ...
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bioRxiv - Cell Biology 2019Quote: Genomic DNA from 60 million unsorted cells (500× coverage of the GeCKO v2 library) and 6 million sorted cells was isolated using Blood & Cell Culture DNA Midi Kit (QIAGEN, 13343) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... and bands at 700 bp (insert) or 6 kb (plasmid library) cut and purified using QiaQuick Gel Extraction Kit (Qiagen #28706) with elution into 30 µL EB.
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bioRxiv - Evolutionary Biology 2021Quote: ... 2.5×106 cells were seeded per well in 6-well plates and transfected using Effectene transfection reagent (Qiagen, Cat. No. 301425). The transfection mixtures in Figure 5A contained 1 μg ...
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bioRxiv - Bioengineering 2021Quote: ... total RNA from the fibroblasts cultured with 10 μM Dwn1 was extracted every 6 hours from hours 24 to 48 (RNeasy® Plus Mini kit, Qiagen), followed by cDNA synthesis (SuperScript® VILO™ cDNA Synthesis Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from untreated cells (24 h) and IFNγ-treated cells (6 or 24 h) were extracted with RNeasy plus mini kit (Qiagen, 74134) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: E3 and E4 primary microglia were plated at 5×106 cells/well in 6-well plates and RNA was extracted from the cells using the RNEasy Plus Mini Kit (Qiagen #74136) and converted to cDNA using High-Capacity RNA-to-cDNA kit (Thermo #4387406 ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from cells in a single well of a 6-well plate was isolated using the RNeasy Plus Mini Kit (Qiagen, 74134) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Transient transfections were carried out using just subconfluent cultures in 35 mm plates or in wells of a 6-well plate using DNA in the range of 0.3-2 µg/culture and the Polyfect reagent (Qiagen, Germantown, MD) and the manufacturer’s protocol ...