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Citations for Qiagen :
451 - 500 of 3295 citations for 6 CHLORO 2 METHYLIMIDAZO 1 2 A PYRIDINE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... DNA was run on 2% agarose gel and extracted using QIAquick Gel Extraction Kit (QIAGEN). Fragments and backbone were ligated using T4 DNA ligase (Cat# M0202 ...
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bioRxiv - Neuroscience 2023Quote: Total mRNA from 2 to 4 organoids were isolated using the RNeasy mini kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The 15 µl reactions each contained 2 × reaction mix from the Multiplex PCR Kit (Qiagen), 5 µg Bovine Serum Albumin ...
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bioRxiv - Microbiology 2023Quote: ... then 50 mg transferred to 2 ml Plant Pro tissue disruption tubes (Qiagen Ltd, U.K.). DNA was extracted following manufacturers protocol ...
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bioRxiv - Genomics 2023Quote: ... RNAse A treatment was conducted by adding 2 uL of RNAse A 100ug/mL (Qiagen) and incubating the samples at 37°C for 15 min ...
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bioRxiv - Genetics 2023Quote: ... we extracted genomic DNA from 2 million cells using DNeasy Blood & Tissue Kit (69504, Qiagen) and performed droplet digital PCR (ddPCR ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was extracted from 2-week old seedlings with DNeasy Plant Mini Kit (Qiagen) and further purified with Isolate II Gel and PCR Clean-up Kit (Bioline) ...
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bioRxiv - Microbiology 2023Quote: Cellular RNA of 2-5×105 cells was extracted using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... with 200 µl of the resulting supernatant transferred into 2 ml EZ1 sample tubes (Qiagen). 12 µl of Proteinase K was added to the supernatant ...
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bioRxiv - Immunology 2023Quote: ... DNA was isolated for each fraction using EZ1&2 DNA Blood 350µL kits (Qiagen, Hilden) and the EZ1 Advanced XL automated system (Qiagen ...
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bioRxiv - Genomics 2023Quote: ... Cell-free RNA was isolated from between 2-8 mL following manufacturer’s instructions (Qiagen, 55114). Isolated RNA was DNase-treated (Lucigen ...
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bioRxiv - Molecular Biology 2023Quote: ... clusters (0-2) were predicted with Ingenuity Pathway Analysis (IPA; version 90348151, Ingenuity Systems, Qiagen), using the cluster marker gene lists (adj ...
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bioRxiv - Microbiology 2024Quote: ... run using the QIAcuity One Digital PCR System (Qiagen, 2-plex Device, Cat. No. 911001). The following conditions were used for the one-step cycling dPCR program ...
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bioRxiv - Microbiology 2024Quote: ... or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0, Qiagen, Toronto, ON, Canada). Then ...
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bioRxiv - Immunology 2024Quote: ... using primers (Supplementary Table 2) and purified the products using QIAquick PCR Purification Kit (Qiagen). PCR-products were Sanger sequenced and knockout scores were determined using the ICE analysis tool (https://ice ...
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bioRxiv - Microbiology 2024Quote: ... or AZD5582 in the presence or absence of KL-2 using an RNeasy kit (Qiagen), with the optional on-column deoxyribonuclease I digestion step ...
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bioRxiv - Immunology 2024Quote: ... Sorted cells (2-4x104 per sample) were resuspended in 350 µl RNeasy Lysis Buffer (QIAGEN), and RNA was extracted using the QIAGEN RNeasy Mini kit (QIAGEN) ...
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bioRxiv - Plant Biology 2020Quote: Reduced His-tagged PRX-IIE (3 mg) or PRX-IIE C146S (3 mg) were bound to 1 mL Ni-NTA resin (Qiagen, Hilden, Germany) and used as affinity matrix ...
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bioRxiv - Neuroscience 2022Quote: Total RNA from 18 ONS cell samples (6 AD patients, 6 MCI and 6 HC) were extracted using Allprep universal kit (Qiagen cat. no. 80224). RNA-seq libraries were prepared using the Illumina TruSeq Stranded Total RNA library Prep Gold Kit (Illumina 20020598 ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM PMSF and the resulting supernatant was incubated with nickel-nitrilotriacetic acid agarose (QIAGEN) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
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bioRxiv - Genomics 2023Quote: ... cfDNA was isolated from 1 mL of plasma using the QIAGEN Circulating Nucleic Acids Kit (QIAGEN), eluted in AE buffer ...
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bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 μg DNA ratio using Effectene transfection reagent (Qiagen). Recombinant luciferase-expressing viruses capable of a single round of replication were released into the cell medium and were harvested 48 h later ...
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bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 µg DNA ratio using effectene transfection reagent (Qiagen). Recombinant ...
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bioRxiv - Systems Biology 2020Quote: ... 6×10^6 PBMC from each sample were transferred directly to Quiazol regent (QIAGEN), resuspended and immediately aliquoted and stored at −80°C until RNAseq downstream processing ...
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bioRxiv - Cell Biology 2021Quote: ... E2D2/3 (5’-AACAGUAAUGGCAGCAUUUGU-3’) and E2D4 siRNAs (5’ – CCGAAUGACAGUCCUUACCAA-3’) all from Qiagen, USA(83).
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bioRxiv - Molecular Biology 2020Quote: 6 ml Ni-NTA (QIAGEN) was washed with 5 column volumes of wash buffer 1 (150 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... HGF (5’-CTCACACCCGCTGGGAGTAC-3’, 5’-TCCTTGACCTTGGATGCATTC-3’), STAT3 (5’-CTTTGAGACCGAGGTGTATCACC-3’, 5’-GGTCAGCATGTTGTACCACAGG-3’) and B-Actin Primer Set (Qiagen, QT00095431). After preparing master mixes samples were prepared in quadruplicate in a 96-well Reaction Microplates (Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... Bound STAT3 proteins were then labeled with Alexa488-conjugated anti-6 × His antibodies (1 hr incubation, 1:20 dilution, Qiagen 35310). The array was washed and scanned using a GenePix 4400A scanner (Molecular Devices ...
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bioRxiv - Genomics 2023Quote: Approximately 3-4 million cells were solubilized with 1 mL QIAzol (Qiagen, 79306) and 0.2 mL chloroform in 5PRIME Phase-Lock Gel heavy tubes (QuantaBio ...
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bioRxiv - Cell Biology 2020Quote: All muscles were homogenized 2 × 30 sec at 30 Hz using a Tissuelyser II (Qiagen, USA) in ice-cold homogenization buffer (10% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg total RNA was used for cDNA synthesis using an Omniscript Reverse Transcription Kit (Qiagen). For the quantitative real-time PCR ...
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bioRxiv - Microbiology 2021Quote: ... except that 2 mL prefilled PowerBead tubes (glass beads, 0.1 mm; Cat no. 13118-50, Qiagen) were used for the bead beating ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein purification was performed using Ni+2-NTA agarose affinity chromatography according to standard protocol (Qiagen).
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bioRxiv - Genetics 2019Quote: ... for lysis in 2 ml safe-lock tubes containing one 5 mm stainless steel bead (Qiagen) for 2.5-3 minutes at 30 Hz using TissueLyzer II (Qiagen) ...
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bioRxiv - Cancer Biology 2019Quote: ... tumor samples (15-30 mg) were transferred to 2 ml round-bottom tubes (Qiagen, Hilden, Germany) with the addition of a 5 mm stainless steel bead (Qiagen ...
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bioRxiv - Systems Biology 2020Quote: ... Cell pellets were transferred to 2 ml polypropylene tubes and disrupted using a TissueLyser II (Qiagen), adding the same volume of acid-washed glass beads (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... as per the manufacturer and a final elution step of 2×10 μL EB buffer (Qiagen).
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bioRxiv - Genomics 2021Quote: ... counted and 2×104 were lysed in 100 µL of lysis buffer (RLT Plus (Qiagen, 1053393) and 1% ß-mercaptoethanol (Sigma-Aldrich,M3148)
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bioRxiv - Microbiology 2021Quote: ... Tissues were homogenized at 30 Hz for 2 min using TissueLyser II (Qiagen GmbH, Hilden, Germany) and centrifuged for 30 sec at 11000 rpm ...
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bioRxiv - Bioengineering 2020Quote: ... tissue clay and control hydrogels were homogenized for 2 minutes (TissueRuptor) in QIAzol lysis Reagant (Qiagen) and chloroform was added to precipitate and remove proteins ...
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bioRxiv - Plant Biology 2021Quote: ... and the resultant supernatant was mixed with 2 ml of Ni-NTA agarose resin suspension (Qiagen) and incubated on a rotary shaker for 60 min at 4°C before transfer to a Poly-Prep Chromatography Column (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen tissue was thawed and immediately homogenized in 2 ml of RTL buffer + 2mM DTT (Qiagen). mRNA was extracted from 300 ul of homogenate using the RNeasy Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was bound to a 2 mL slurry of Strep-Tactin Superflow plus (Qiagen) overnight at 4°C using the batch method ...
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bioRxiv - Physiology 2021Quote: ... Pooled libraries were diluted to 2 nM in EB buffer (Qiagen, Hilden, Germany, Cat. No. 19086) and then denatured using the Illumina protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Protein extract was subjected to IMAC by incubation with 2 ml Ni-NTA resin (Qiagen, Germany) per 50 ml of extract ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 15 mg/mL of lysozyme and 2 mg/mL of proteinase K (Qiagen), and incubated at RT for 10 min ...
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bioRxiv - Immunology 2022Quote: ... Each 25 µL qPCR reaction contained 12.5 µL of 2× QuantiTect Probe PCR Master Mix (QIAGEN), 600nM of each primer ...
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bioRxiv - Neuroscience 2022Quote: ... CD11b+ CD45+ cells from one animal (2 retinas) were sorted directly into RLT buffer (QIAGEN 79216) and stored at -80°C ...
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bioRxiv - Genomics 2022Quote: ... Purified PCR products (400 ng) were mixed with 2 μL 10X Taq polymerase PCR buffer (QIAGEN) and nuclease free water to a final volume of 20 μL ...