Labshake search
Citations for Qiagen :
601 - 650 of 2334 citations for 6 Benzofurancarboxamide N 1R 3R 4S 1 azabicyclo 2.2.1 hept 3 yl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Two glass beads were added before shaking for 3 minutes using a Tissue Lyser (QIAGEN) at maximum speed ...
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bioRxiv - Cancer Biology 2023Quote: ... Equivalent cell numbers (∼3 × 106) across all samples were harvested in QIAzol lysis reagent (Qiagen). Illumina gene expression raw data were transformed using variance- stabilizing transformation (VST ...
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bioRxiv - Genomics 2023Quote: ... 3) cleanup of final libraries with QIAgen MinElute PCR purification kit (QIAgen, Cat. No 28004), followed by a second cleanup with 2X SPRI ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated for 3 minutes at 56°C in a deparaffinization solution (Qiagen, 19093). A step of digestion with Proteinase K was performed followed by a treatment with DNAses ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was then incubated with 3 ml of Ni-NTA Superflow agarose beads (QIAGEN), which were preequilibrated with bacterial lysis buffer ...
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bioRxiv - Immunology 2024Quote: ... Total RNA (3 independent samples per group) was isolated (QIAGEN miRNeasy mini extraction kit (QIAGEN) and cDNA was synthesized (High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems)) ...
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bioRxiv - Immunology 2024Quote: ... Total RNA (3 independent samples per group) was isolated (QIAGEN miRNeasy mini extraction kit (QIAGEN) and cDNA was synthesized (High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems)) ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was briefly incubated (approximately 5 min) with 3 ml Ni-NTA Agarose (QIAGEN) before flow-through was collected ...
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bioRxiv - Neuroscience 2022Quote: ... and aged (n=10) RVLM-containing tissue punches was reverse transcribed to cDNA using an RT2 preamp cDNA synthesis kit (Qiagen, Inc., First strand cDNA synthesis) following manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from whole brain of 6 dpf larval zebrafish using RNeasy micro plus RNA extraction kit (Qiagen, Germany). Total RNA (2ng/µl ...
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bioRxiv - Cell Biology 2019Quote: Genomic DNA from 60 million unsorted cells (500× coverage of the GeCKO v2 library) and 6 million sorted cells was isolated using Blood & Cell Culture DNA Midi Kit (QIAGEN, 13343) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... and bands at 700 bp (insert) or 6 kb (plasmid library) cut and purified using QiaQuick Gel Extraction Kit (Qiagen #28706) with elution into 30 µL EB.
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bioRxiv - Evolutionary Biology 2021Quote: ... 2.5×106 cells were seeded per well in 6-well plates and transfected using Effectene transfection reagent (Qiagen, Cat. No. 301425). The transfection mixtures in Figure 5A contained 1 μg ...
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bioRxiv - Bioengineering 2021Quote: ... total RNA from the fibroblasts cultured with 10 μM Dwn1 was extracted every 6 hours from hours 24 to 48 (RNeasy® Plus Mini kit, Qiagen), followed by cDNA synthesis (SuperScript® VILO™ cDNA Synthesis Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from untreated cells (24 h) and IFNγ-treated cells (6 or 24 h) were extracted with RNeasy plus mini kit (Qiagen, 74134) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: E3 and E4 primary microglia were plated at 5×106 cells/well in 6-well plates and RNA was extracted from the cells using the RNEasy Plus Mini Kit (Qiagen #74136) and converted to cDNA using High-Capacity RNA-to-cDNA kit (Thermo #4387406 ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from cells in a single well of a 6-well plate was isolated using the RNeasy Plus Mini Kit (Qiagen, 74134) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Transient transfections were carried out using just subconfluent cultures in 35 mm plates or in wells of a 6-well plate using DNA in the range of 0.3-2 µg/culture and the Polyfect reagent (Qiagen, Germantown, MD) and the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: RNA from myotubes grown in 6-well plates in conditions described above was isolated using the RNeasy Micro Kit from Qiagen (#74004). Quality of RNA was assessed by using a Bioanalyzer 2100 and samples were submitted for library preparation and deep sequencing to the Molecular biology core facility at the Dana Farber Cancer Institute ...
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bioRxiv - Bioengineering 2019Quote: ... The inclusion body pellets were resuspended in 20 mM Tris-HCl buffer with 500 mM sodium chloride and 6 M urea (pH=8.0) and incubated with Ni-NTA resin (Qiagen, Valencia, CA) for 2 hours at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was isolated from iPSC-SNs seeded in 6-well plates using a RNeasy Mini Kit (Qiagen, Redwood City, CA) and reverse transcribed into cDNA using a SuperScript VILO cDNA Synthesis kit (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown to 80% confluence in a 6-well plate before RNA was extracted using standard RNA extraction protocol (RNAeasy Mini Kit, Qiagen, #74104). cDNA synthesis was performed by mixing 1μg of RNA with qScript® cDNA Synthesis Kit (Quantabio ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from GWSS and control samples (nGWSS = 79, ncontrol = 6) using a DNeasy PowerSoil DNA Isolation kit (Qiagen, Germany) with minor changes to the manufacturer’s protocol as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... BON1/TGL/pQ and BON1/TGL/HA-Bcl-xL) grown on 6-cm dishes were used to isolate mRNA using RNeasy Plus Mini kit (Qiagen, 74136) containing gDNA Eliminator spin columns ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was isolated from cell pellets in batches of 6 using an RNeasy Plus Mini Kit (Cat # 74134, Qiagen, Hilden, Germany). Acceptable RNA concentration and quality were verified with Nanodrop and Bioanalyzer measurements ...
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bioRxiv - Bioengineering 2023Quote: ... DNA was extracted in pools of 6-8 adult mosquitoes mosquitoes using Blood & Cell Culture DNA Midi Kit (Qiagen, Cat# 13343) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... in vitro derived Foxp3+ cells were harvested on day 2 and day 6 into Trizol and total RNA was isolated with miRNeasy Micro Kits (QIAGEN 217084) according to the manufacturer’s instructions ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... RNA and protein were extracted 6 days post-transduction using the AllPrep RNA/Protein kit (Cat: 80204, Qiagen, Germantown, MD USA). RNA was converted to cDNA using SuperScript IV cDNA Synthesis Kit (Cat ...
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bioRxiv - Evolutionary Biology 2023Quote: ... trigynum (Sample size: L. tenue thrum=6, pin=4, L. trigynum homostyle=5) using the RNeasy Plant Mini Kit (QIAGEN, Germany). Libraries were sequenced on an Illumina NovaSeq S1 Sequencing System to produce paired-end 150bp read length reads ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μL of 10 μM double-stranded mature miRNA mimic (Dharmacon) or 20 μM LNA (Qiagen) was mixed with 3 μL lipofectamine RNAiMAX in 100 μL Opti-MEM reduced serum media and added to cells following a 15-minute incubation ...
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bioRxiv - Plant Biology 2020Quote: Total DNA was isolated from 2-3 weeks old seedlings with DNeasy Plant Mini Kit (QIAGEN). 1ng of DNA per qPCR reaction was used as template ...
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bioRxiv - Developmental Biology 2022Quote: The total RNA of 3-4 organoids was extracted using the RNeasy Plus Micro Kit (Qiagen), followed by reverse transcription to generate cDNA with GoScript Reverse Transcription Kit (Promega) ...
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bioRxiv - Genetics 2019Quote: Total RNA from whole 3 day old larvae was extracted using the RNeasy mini kit (Qiagen) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Total RNA samples obtained from 3 biological replicates were purified using RNeasy Mini Kit (#74104, Qiagen) according the manufacturer instructions with a DNase digestion step (#79254 ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated from control and GDC-RPC-3 cells using the RNeasy PlusMini Kit (Qiagen). High-quality (Agilent Bioanalyzer RIN>7.0 ...
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bioRxiv - Microbiology 2022Quote: ... The soluble extracts were mixed for 30 min with 3 ml of Ni2+-NTA-agarose (Qiagen) that had been equilibrated with buffer A containing 10 mM imidazole ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 million cells were seeded and transfected with 1μg pMK33-based plasmid DNA using Effectene (Qiagen). After 48 hours ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from 3 million transfected cells using the All Prep DNA/RNA kit (Qiagen) and quantified by Nanodrop 1000 ...
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bioRxiv - Microbiology 2020Quote: The 3 Kb mutagenic DNA fragments were purified using a QIAquick PCR Purification Kit (Qiagen; 28106) and transformed into V ...
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bioRxiv - Molecular Biology 2019Quote: ... 3 μL water and 5 μL 2x QuantiFast® SYBR® Green PCR Master Mix (Qiagen). Technical duplicates of this reaction mix were then analyzed on a Corbett Rotor-Gene 6000 real-time PCR cycler ...
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bioRxiv - Biochemistry 2020Quote: ... and pJET_Luc_FL_UTR_REV (5’-GCAATGAAAATAAATGTTTTTTATTAGGCAGAATCCAAATGC-3’) primers and was purified with the QIAquick PCR Purification Kit (Qiagen) directly after the PCR reaction ...
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bioRxiv - Genetics 2021Quote: RNA was extracted from an aliquot of 3 × 106 cells using the RNeasy Plus Kit (Qiagen). cDNA was synthesized from extracted RNA using the iScript cDNA Synthesis Kit (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... Media and osteoblasts were harvested 3 days later and EVs were collected using Exoeasy kit (Qiagen). Total RNA was extracted using miRNeasy micro (Qiagen).
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bioRxiv - Cancer Biology 2021Quote: ... RNA from 3 x 106 HMC-1.2 cells was extracted using RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... A Locked Nucleic Acid (LNA) probe was used to detect centromere regions (5’-TTGGCTACACCATGAAAGCTT-3’; Qiagen). 20 μL of probe mix (250 nM LNA probe ...
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bioRxiv - Genetics 2022Quote: ... 3 min in 37°C shaker) and processed with DNeasy Blood and Tissue Kit (QIAGEN #69504). Two hundred ng of genomic DNA was used as input in the DamID protocol that included an improved pool of AdR primers as described (de la Cruz Ruiz et al ...
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bioRxiv - Biochemistry 2023Quote: The total RNA was extracted from 3 × 106 HEK293T cells using the RNeasy Mini Kit (QIAGEN). RNA served as a template for subsequent reverse transcription into cDNA by iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... whole eyes were homogenized in PBS using a TissueLyser II (Qiagen, 30 Hz for 3 minutes), and homogenates were serially diluted and streaked on LB plates for quantification of colony forming units (CFU ...
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bioRxiv - Microbiology 2023Quote: ... Samples were mechanically disrupted for 2x 3 min at 30 Hz (TissueLyser II, Qiagen, Hilden, Germany). This was followed by adding 5 μl of proteinase K (20 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatant was added to 3 ml of Ni-nitrilotriacetic acid (NTA) superflow resin (Qiagen) and the mixture was applied onto a gravity drip column ...