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Citations for Qiagen :
1 - 50 of 5273 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... plasmids bearing chimeric antibody heavy and light chain genes were prepared (EndoFree Plasmid Mega prep Kit, QIAGEN) and transfected into Human Embryonic Kidney cells (HEK293F ...
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bioRxiv - Cell Biology 2022Quote: ... Purification of His6-light chain TeTx was done using Ni-NTA resin (Qiagen) in 50 mM Tris HCl pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... cDNA products were then used in nested PCR for heavy/light chain variable region amplification with HotStarTaq Plus DNA Polymerase (QIAGEN # 203643) and specific primer sets described previously 34,35 ...
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bioRxiv - Immunology 2021Quote: ... We then amplified heavy and light chain sequences from the WTA products utilizing pools of partially degenerate pools of V region specific primers (Qiagen HotStar Taq Plus). Heavy and light chain amplifications were carried out separately ...
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bioRxiv - Biophysics 2020Quote: ... the surface was incubated with 1 nM biotinylated penta-His antibody (Qiagen) in buffer A for 10 min ...
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bioRxiv - Biophysics 2021Quote: ... gliding motility assays were performed with specific immobilization of motors on the surface via penta-His antibodies (34660, Qiagen) as previously described[64] (Figure S4) ...
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bioRxiv - Synthetic Biology 2020Quote: ... For SARS-CoV-2 antigen detection the blots were incubated with 1:2000 Anti-His mouse monoclonal primary antibody (Qiagen, #34660) and then incubated with 1:10000 peroxidase labelled anti-mouse IgG secondary antibody (GE Healthcare) ...
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bioRxiv - Genetics 2021Quote: Relative telomere length of MDS patients was measured by quantitative polymerase chain reaction (qPCR) as previously reported.8 K562 cell DNA was extracted using the QIAamp Blood Mini kit (Qiagen). Telomere length was measured by two orthogonal methods ...
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bioRxiv - Cell Biology 2022Quote: ... Drosophila S2 cells were transfected with mNeonGreen-tagged kinesin-1 full-length heavy chain (KHC) and Halo-tagged PLP584-1811 (Effectene, QIAGEN). The lysates were harvested for TIRF motility assays at ∼48 h post-transfection ...
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bioRxiv - Immunology 2024Quote: ... Recombinant DENV EDIII antigens were expressed in mammalian Expi293 cells and purified from the culture supernatant using Ni-NTA agarose (Qiagen). Purified EDIII antigens were biotinylated using HaloTag PEG biotin ligand (Promega) ...
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bioRxiv - Immunology 2022Quote: ... Enriched cells were surface stained and 5-20 ×103 intratumoral cDC1s were sorted directly in RLT lysis buffer (Qiagen) containing 2-mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: ... the variable region genes from each single cell were amplified in a reverse transcriptase polymerase chain reaction (RT-PCR: QIAGEN, Germany) using a cocktail of sense primers specific for the leader region and antisense primers to the Cγ constant region for heavy chain and Cκ and Cγ for light chain ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from leaf samples of the control and treated (chilling, high light and chilling plus high light) plants using the RNeasy Plant Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Polymerase chain reaction (PCR) was performed with the HotStarTaq Plus polymerase (Qiagen) with 5 min initial denaturation at 94°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were grown to approximately 80% confluency in light and heavy media and transfected using Attractene (#301007, Qiagen, Germany) with SBP-empty vector (control cells ...
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bioRxiv - Microbiology 2020Quote: Polymerase chain reaction (PCR) was performed using Taq DNA Polymerase (QIAGEN, Hilden, Germany). The fungal ITS-28S rRNA gene region was obtained using the ITS5 (White et al. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... QuantiTect Probe RT-PCR (reverse transcription polymerase chain reaction) Kit (QIAGEN, Hilden, Germany) was used to perform RRT-PCR reactions in the ABI 7300 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2023Quote: Polymerase Chain Reactions (PCRs) were performed using Type-it Microsatellite PCR Kit (Qiagen) in 10 µl reactions ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from mammalian cell culture after 48 h of pulsatile light activation using RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Transfected cells were subjected to 48 h of pulsatile light activation and total RNA was extracted from cells using RNeasy Plus Mini Kit (QIAGEN) and QIAshredder (QIAGEN ...
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bioRxiv - Physiology 2023Quote: ... and real-time polymerase chain reaction (PCR) was performed with Fast SYBR Green (QIAGEN). Gapdh was used as the housekeeping gene ...
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bioRxiv - Physiology 2020Quote: ... The polymerase chain reaction (PCR) were carried out using Rotor Gene Q (Qiagen, Hilden, Germany) with initial incubation at 95 °C for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction was conducted using miRCURY LNA miRNA SYBR PCR kit (Qiagen #339345) as per manufacturer’s instruction on BioRad CFX96™ System in 96-well plates in 3-6 repeats ...
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bioRxiv - Molecular Biology 2021Quote: Polymerase chain reaction (PCR) products were gel purified using Qiagen QlAquick gel purification columns (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative real-time polymerase chain reactions were performed using the QuantiFast SYBR Green PCR Kit (QIAGEN) and the CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Transgenic animals were identified by polymerase chain reaction of tail DNA (DNeasy kit, Qiagen, Hilden, Germany) using primers for Cre (Schönig et al. ...
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bioRxiv - Cell Biology 2022Quote: ... in a Rotor-Gene® Q real-time polymerase chain reaction machine (Qiagen Inc., Germantown, MD). The PCR primers were designed with Primer Premier 6 (Premier Biosoft Int ...
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Immune profiling in M. tuberculosis infection enables stratification of patients with active diseasebioRxiv - Immunology 2019Quote: ... 1mL of whole blood was collected directly in QFT Gold tubes (Nil, TB Antigens, Mitogen) (Qiagen) according to manufacturers’ instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Extracted DNA was used for genotyping by polymerase chain reaction (PCR) (Taq DNA polymerase, Qiagen, Hilden, Germay), using primers flanking the endogenous CAG repeat in exon 10 of the murine Atxn3 gene (primer sequences ...
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bioRxiv - Genomics 2020Quote: ... Crypts were then separated under a light microscope and placed in ATL buffer (Qiagen) containing 10%(v/v ...
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bioRxiv - Cancer Biology 2019Quote: The cDNA of wild-type ecATCase and regulatory chain of ecATCase-holo were amplified by PCR (Qiagen Kit) using BL21(DE3 ...
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bioRxiv - Immunology 2021Quote: ... was then added to a polymerase chain reaction (PCR) mix containing 12.5 μl of 2× Multiplex PCR mix (Qiagen), 9 μl of H2O ...
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bioRxiv - Microbiology 2020Quote: ... Insertion of the dcas9/lacI cassette at the desired chromosomal location in strain CJW_Bb362 was confirmed by polymerase-chain reaction (PCR) analysis of isolated total genomic DNA (DNeasy Blood & Tissue Kit, Qiagen), using the following primer pairs ...
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bioRxiv - Genetics 2020Quote: ... Multiplex polymerase chain reaction (PCR) was performed using three primer pairs and the Multiplex PCR Kit (QIAGEN, Hilden, Germany). Each reaction contained 2× QIAGEN multiplex PCR master mix ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments that were generated by the polymerase chain reaction (PCR) were purified using the PCR Purification Kit (Qiagen), Monarch® PCR & DNA Cleanup Kit (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... photo-activated using two light sources (Halogen lamp described above or BLU-V System – Qiagen) and stored at −80°C until DNA extraction with a magnetic-based method (MagMAX CORE extraction kit – ThermoFisher).
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bioRxiv - Microbiology 2021Quote: DNA was isolated from soil and surface sample filters using the DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) and from water sample filters with the DNA-EZ ST01 Kit (GeneRite ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out using a QuantiFast SYBR Green PCR kit (QIAGEN, 204056) on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... One-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) following the standard protocol for a QuantStudio 7 Flex RT-PCR system ...
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bioRxiv - Biochemistry 2020Quote: ... low melting point agarose gel and the approximately 700 base pair band corresponding to the heavy chain only immunoglobulin was extracted and purified using QIAquick Gel Extraction Kit (Qiagen). A secondary amplification of the VHH domain was performed using modified primers (VHH-Esp-For ...
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bioRxiv - Microbiology 2020Quote: ... Gene expression was assessed using SYBR GreenER based-quantitative polymerase chain reaction (qPCR) or RT2 profiler arrays (Qiagen Ltd, UK). For SYBR GreenER based-qPCR analyses ...
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bioRxiv - Microbiology 2020Quote: Quantitative polymerase chain reaction (qPCR) analyses were performed on the new DNA extracts using PowerSoil DNA Isolation Kit (12888-50, QIAGEN) to estimate the abundance of bacteria and archaea at different depths in the sediment core ...
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bioRxiv - Molecular Biology 2022Quote: ... This 1st round IgK PCR reaction was used for the 2nd round PCR of IgK chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The same cDNA reaction was also used for the 1st round PCR of IgK chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... This 1st round IgG PCR reaction was used for the 2nd round PCR of IgG chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... This cDNA reaction was used for the 1st round PCR of IgG chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
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bioRxiv - Bioengineering 2021Quote: ... One-step reverse transcription quantitative polymerase chain reaction (RT-qPCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) with 7.8 μl purified viral RNA or specified quantities (2.5 ng or 100 ng ...
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bioRxiv - Neuroscience 2022Quote: ... The heavy-chain specific amplicon was isolated using electrophoresis with low-melting point agarose extraction with the QIAquick Gel Extraction kit (Qiagen). A secondary amplification was performed using a modification of the primers (VHH-Esp-For ...
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bioRxiv - Bioengineering 2023Quote: Reverse transcription of isolated miRNAs for quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed with a miScript II RT Kit (Qiagen) for selected miRNA targets ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Light organs were then transferred into 180 ul of pre-heated (98°C) ATL buffer (QIAGEN) and incubated at 98°C for 15 minutes ...