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Citations for Qiagen :
151 - 200 of 329 citations for 26S Proteasome Non ATPase Regulatory Subunit 11 PSMD11 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: Total RNA was isolated from leaf tissue of transgenic and non-transgenic plants using the RNeasy Mini Kit (Qiagen) following the manufacturer’s instructions and treated with RNase free-DNase (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: 2.0 × 105 HeLa cells were transfected in 12 well plates with control (NTC: all-stars non-targeting control, Qiagen) or DDX39B-targeting (D11 ...
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bioRxiv - Cancer Biology 2022Quote: RNA was extracted from dcas9-KRAB-MCF7 cells transduced with targeting and non-targeting sgRNA (Qiagen, cat no. 74016). RNA was retrotranscribed using iScript (BioRad ...
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bioRxiv - Immunology 2022Quote: ... proliferated (eFluor670low) and non-proliferated (eFluor670high) fractions were sort purified and RNA extraction was performed (miRNeasy micro-Kit, Qiagen). After cDNA synthesis (High capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Cell Biology 2019Quote: ... accordingly to manufacturer’s instructions using non-targeting siRNA (siCtrl; 5’-AATTCTCCGAACGTGTCACGT-3’) and siRNA targeting Cav1 (SI00299635 and SI00299628) from Qiagen, or using pre-miR-NC (negative control ...
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bioRxiv - Cell Biology 2019Quote: ... Genomic DNA was isolated from edited clones and non-edited control cells using the DNeasy Blood & Tissue Kit (Qiagen). RAD51 paralog specific loci were PCR amplified using gene specific PCR primers Table S4 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA targets were only included if biochemically confirmed using human tissue or non-species specific methods (sourced from QIAGEN’s curated Ingenuity Knowledge Base[48] ...
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bioRxiv - Genomics 2021Quote: ... Long range PCR amplicons and non-enriched DNA were further purified using the QIAEX II Gel extraction Kit (Qiagen). Due to indispensable loss of HCMV-DNA (>80% ...
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bioRxiv - Genetics 2020Quote: ... and cells treated with transfection mix without siRNA (Wild type (WT)) or non-targeting control siRNA (scrambled (SCR)) (Qiagen catalog SI03650318 ...
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bioRxiv - Cell Biology 2022Quote: siRNA targeting ARRB2 (ARRB2-12, Cat #SI03099103) and a non-targeting control (AllStars, Cat # 1027415) was purchased from Qiagen. A pool of 4 siRNAs targeting human RME-8/DNAJC13 was purchased from Dharmacon/Horizon Discovery (Cat #L-010651-01 ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA from non-FFPE tissues was extracted using AllPrep DNA/RNA/miRNA Universal Kit (Cat. No. / ID: 80224, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were harvested through centrifugation at 7025 x g and re-suspended in non-denaturing lysis buffer (Qiagen) containing 10 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was extracted from isolated cells (microglia or non-microglia cells) with RNeasy RNA Isolation kit (Qiagen 74104) and used to generate cDNA with a High-Capacity cDNA Reverse Transcription Kit (Thermo 4368814) ...
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bioRxiv - Cell Biology 2022Quote: ... Non-targeting siRNA controls contained 20 nM (for ATGL) or 40 nM (for cPLA2α) AllStars Negative Control siRNA (Qiagen). Transfection complexes were generated using 1 μL/well Lipofectamine RNAiMAX in 24-well plates ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with 200 nM of ON-TARGETplus SMARTpool siRNA targeting TUBB6 (Table S11) or the ON-TARGETplus Non-targeting control pool (Dharmacon) using HiPerfect transfection system (Qiagen) in RPMI ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA and RNA were extracted from peripheral blood leukocytes from patient 6003 and from a non-HHT control individual using the Gentra PureGene Blood Kit (Qiagen) and TRIzol Reagent (Invitrogen ...
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bioRxiv - Immunology 2019Quote: Total RNA was extracted from 5 dpf infected and non infected snap-frozen larvae (20 larvae/sample) from three independent crosses using Qiazol reagent (79306, QIAGEN) according to the manufacturer’s instructions and extracted with RNeasy Mini kit (74104 ...
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bioRxiv - Cell Biology 2021Quote: ... Disruption of RAD54 and RAD54B was validated by sequence analysis after genomic DNA was isolated from a selection of edited and non-edited clonal isolates using DNeasy Blood & Tissue Kit (Qiagen). RAD54 and RAD54B genomic DNA sequences were amplified by PCR using primer pairs flanking the sgRNA target sites (Supplemental Table S3) ...
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bioRxiv - Cell Biology 2021Quote: siRNAs against RAB5A(S100301588), RAB5B (S102662800), RAB5C(S102663073), RAB8A (SI02662254), RAB8B(SI02662261), RAB10 (SI00301546, SI03037076) and non-targeting control were purchased from Qiagen AS (FlexiTube siRNA) ...
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bioRxiv - Evolutionary Biology 2022Quote: We collected blood samples non-destructively from mistnetted wild birds (Table S6) and extracted DNA using DNeasy Tissue Extraction Kits (Qiagen) or standard phenol-chloroform protocol45.
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bioRxiv - Neuroscience 2020Quote: ... 20 ng of genomic DNA derived from sorted neuronal and non-neuronal nuclei of a postmortem human ACC sample (Brain A) was used for bisulfite conversion (EpiTect Bisulfite Kit, Qiagen). For each sample ...
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bioRxiv - Cell Biology 2019Quote: ... human primary monocytes were transfected with 200 nM of ON-TARGETplus SMARTpool siRNA targeting Siglec-1 (Horizon Discovery) or non-targeting siRNA (control) using HiPerfect transfection system (Qiagen). Four hours post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... iniae QMA0248 grown for 18 h on Todd-Hewitt agar from a master seed stock (non-subcultured) with the Genomic Tip 20 kit (Qiagen). Pre-incubation for 2 h at 37°C of cells suspended in 500 μL 50 mM EDTA containing 200 units of mutanolysin and 2 mg lysozyme was found to improve cell lysis prior to following the manufacturers protocol for purification of high molecular weight DNA ...
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bioRxiv - Genomics 2019Quote: ... Briefly, mouse ESCs treated with non-targeting control siRNA (Dharmacon, D-001810-02-50)or NF-YA siRNA (Qiagen, SI01327193) were collected 48 hours post-transfection in cold PBS ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA from non-sorted (exponentially growing or stationary) cells was also extracted using Blood and Tissue DNA extraction kit (Qiagen), following the manufacture instructions.
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bioRxiv - Microbiology 2020Quote: ... The eluant was treated with DNase I (1 U/μl) to digest non-specific DNA and the bound DNA was purified from the complex via PCR Qiagen Kit (Qiagen). The purified DNA was amplified with primer sets specific to the prrF1,F2 promoter (Table S2 ...
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bioRxiv - Neuroscience 2023Quote: Samples were collected from the injured cortical region (including affected as well as non-affected brain tissue) and homogenized using the TissueLyser (QIAGEN). Total RNA was extracted with TRI Reagent (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Disruption of RAD51AP1 or RAD54L was validated by sequence analysis after genomic DNA was isolated from a selection of edited and non-edited clonal isolates using DNeasy Blood & Tissue Kit (Qiagen). RAD51AP1 and RAD54L genomic DNA sequences were amplified by PCR primers flanking the sgRNA target sites (Table S4) ...
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bioRxiv - Molecular Biology 2023Quote: Primary monocytes were cultured in 24-well plates and then transfected with control non-targeting siRNA (Dharmacon) or siRNA targeting ERN1 (Dharmacon) using HiPerFect reagent (QIAGEN) following the instructions provided by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... TbFUT1 cKO and TbFUT1 cKO F1-γL262P/WT cells grown under permissive and non-permissive conditions and RNA was extracted from 1 ×107 cells using RNeasy RNA extraction kit (Qiagen). RT-qPCRs were performed using Luna® Universal qPCR Master Mix (NEB ...
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bioRxiv - Genetics 2023Quote: All cDNA samples were pooled into two groups corresponding to cases (alcohol drinkers) and controls (non-drinkers) and then assayed using the Serum/Plasma Focus PCR panel (Qiagen). This commercially available microarray platform contains 179 LNA miRNA primer sets of miRNAs that have been commonly found in human plasma ...
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bioRxiv - Developmental Biology 2023Quote: ... Specific predesigned Silencer-Select gene siRNAs (ThermoFisher Scientific; Eif4g - s101902, Larp1 - s91534, Ddx21 - s80158 or All-Stars negative murine control non-targeting control/NTC, from Qiagen were microinjected at a final concentration of 10µM each) ...
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bioRxiv - Cell Biology 2023Quote: ... ULK2 (mixture of 4 siRNAs, GS9706), TBC1D14 (mixture of 4 siRNAs, GS57533) and non-targeting (NT, # 5091027310) were purchased from Qiagen.
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bioRxiv - Biochemistry 2023Quote: ... HBEC5i cells with treated with either non-specific siRNA or Flvcr2 siRNA were harvested and kept in 1 mL TRI reagent (Qiagen).
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bioRxiv - Plant Biology 2024Quote: ... from infected leaf material or from urediniospores of non-target rust species (Table 2) with the DNeasy Plant mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from whole lungs from IAV non-infected / infected mice and human PBMCs using (RNeasy mini kit, Qiagen). RNA quantity and purity were assessed using a spectrophotometer (NanoDrop) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... dispar we used 5 mm pieces of fin clip from each individual sample for 28-29 individuals per site (at 11 sites) to extract DNA with 96-well DNA extraction kits from Qiagen (DNeasy 96 Blood and Tissue Kit) or Macherey-Nagel (NucleoSpin 96 Tissue) ...
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bioRxiv - Immunology 2021Quote: ARNO siRNA (Mm_Pscd2_3) or a negative non-specific siRNA control (Allstar siRNA) were transfected into SFs using HiPerFect transfection reagent (all Qiagen,UK) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... quantitative RT-PCR, and immunoblotting. For knock-down experiments, non-specific (siNS, No. 1027310) or AHR (siAHR, SI02780148) siRNA (QIAGEN, Inc.) was transfected with LipofectamineTM RNAiMAX (Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2020Quote: HACs from six non-OA donors (Supplementary File 2) seeded individually at 30,000 cells/cm2 in triplicate were transfected (HiPerfect; Qiagen, Manchester, UK) with 100 nM ASO (Eurogentec ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted either from shed skins or non-invasive blood samples (Supplementary Table S1) using PureGene® or DNeasy Blood and Tissue Kits (Qiagen), and genomic DNA stored at −20LC ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from fibroblasts obtained from each individual mouse from each of the five non-CC inbred strains using AllPrep DNA/RNA Mini kit (Qiagen, #80204). The concentration and purity of extracted DNA was determined using a NanoDrop 2000 UV-vis spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... or a non-targeting scrambled control (Scr; sequence: 5’-TAACACGTCTATACGCCCA-3; Qiagen, Cat No.: 339137; 0.1 nmol in 2 μL PBS), using a 2 μL Hamilton syringe at a rate of 1 μL/min ...
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bioRxiv - Physiology 2022Quote: ... nuclear DNA was isolated from the sorted myonuclear and non-myonuclear fractions using QIAmp DNA Micro Kit (Qiagen, Germantown, MD, USA) with adjusted reagents’ volumes to the samples size ...
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bioRxiv - Microbiology 2020Quote: ... and each amoeba-adapted and non-adapted population derived from days 3 and 42 using the QIAamp DNA mini kit (Qiagen, Venlo, Netherlands) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... was performed on the genomic DNAs (gDNAs) isolated from peripheral blood of non-obstructive azoospermia (NOA) patients using the QIAamp DNA Blood Mini Kit (51206; Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: The LNA GapmeR oligonucleotides specifically targeting SAMMSON and the LNA non-targeting control (NTC) GapmeR were purchased from Qiagen (catalog number 339517).
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bioRxiv - Biochemistry 2020Quote: Total RNAs were extracted from Wildtype MIN6 and MIN6 stably expressing shRNAs (non-specific/NS, C, D) using RNeasy kit (QIAGEN, Germantown, MD) and cDNAs were prepared using qScript cDNA SuperMix (Quantabio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Ceroptres samples used only for mtCOI sequencing were non-destructively sampled using a QIAGEN DNeasy Blood and Tissue Kit (QIAGEN, Valencia, CA, USA) and deposited as vouchers (Table S2) ...
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bioRxiv - Neuroscience 2022Quote: ... to reduce the α-synuclein expression and a non-targeted LNA (ntLNA) as control (sequence: AACACGTCTATACGC). Antisense LNA GapmeR (cat. 339511) were purchased from QIAGEN (Qiagen N.V., Venlo, Netherlands). From DIV 1 on ...