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Citations for Qiagen :
1 - 50 of 1468 citations for 2 Amino 4 bromobenzyl alcohol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted from alcohol-preserved tissues using the DNeasy Blood and Tissue kit (QIAGEN). Tlr genes were genotyped using a two-step approach ...
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bioRxiv - Cancer Biology 2023Quote: ... After chloroform: isoamyl alcohol (24:1) extraction followed by purification (Qiagen RNeasy Mini Kit, 74104), the biotinylated RNA was resuspended in water and biotinylated-RNA was isolated using Dynabeads MyOne Streptavidin C1 magnetic beads (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... using a TissueLyser (2 × 30 Hz, 3 min at 4°C; QIAGEN). After homogenization ...
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bioRxiv - Neuroscience 2024Quote: ... using a TissueLyser (2 × 30 Hz, 3 min at 4°C; QIAGEN). After homogenization ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 2-4 ml of cells were harvested at each timepoint and incubated with 2 volumes of RNAprotect (Qiagen) at room temperature for 5 minutes prior to centrifugation for 5 minutes at 4000xg 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The protein was eluted with 3C protease73 and subsequently incubated at 4°C for 2 h with 4 mL of Strep-tactin Superflow Plus beads (Qiagen) pre-equilibrated with buffer E ...
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bioRxiv - Biophysics 2022Quote: ... The protein was eluted with 3C protease40 and subsequently incubated at 4°C for 2 h with 4 mL of Strep-tactin Superflow Plus beads (Qiagen) pre-equilibrated with buffer E ...
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bioRxiv - Molecular Biology 2021Quote: ... Unbound biotin was removed by performing a chloroform:isoamyl alcohol extraction using MaXtract High Density tubes (Qiagen). RNA was isopropanol precipitated and resuspended in water ...
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bioRxiv - Microbiology 2022Quote: ... Unbound biotin was removed by performing a chloroform:isoamyl alcohol extraction using MaXtract High Density tubes (Qiagen). RNA was isopropanol precipitated and resuspended in water ...
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bioRxiv - Microbiology 2023Quote: ... Unbound biotin was removed by performing a chloroform:isoamyl alcohol extraction using MaXtract High Density tubes (Qiagen). RNA was isopropanol precipitated and resuspended in water ...
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bioRxiv - Microbiology 2020Quote: ... 2 mL of fermentor culture was added to 4 mL RNAprotect Bacteria Reagent (Qiagen) and immediately vortexed for 10 sec ...
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bioRxiv - Genomics 2021Quote: ... DNA purification was done with phenol/chloroform/isoamyl alcohol extraction followed by chloroform extraction using MaxTract tubes (Qiagen). DNA was precipitated with ethanol after addition of 20 µg glycogene ...
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bioRxiv - Immunology 2023Quote: ... Bacterial gDNA were extracted from the liquid cultures using phenol:chloroform:isoamyl alcohol and then purified further with the QIAquick PCR Purification kit (Qiagen). Prevotella-specific 16S rRNA gene primers (gPrevo-F and R-)14 (Table S3 ...
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bioRxiv - Immunology 2023Quote: Bacterial genomic DNA from frozen stool samples was extracted using phenol:chloroform:isoamyl alcohol and then purified further with the QIAquick PCR Purification kit (Qiagen)45 ...
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bioRxiv - Systems Biology 2024Quote: Fragments were purified by adding 300µL phenol:chloroform:isoamyl alcohol (25:24:1 v/v) and sample to a phase lock tube (Qiagen #129046), and spun down at 16,000g for 3 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... 4 μl of bisDNA was added to 2 μl 10x PCR buffer (Qiagen, Cat# 203203), 2 μl of 10 μM primer mix (Methods Table 1) ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was incubated for 2 h at 4 °C with Ni2+-NTA-agarose (Qiagen) (20 mg of proteins/ml of resin ...
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bioRxiv - Immunology 2021Quote: ... Skin samples were homogenized in a TissueLyser LT (Qiagen, 50 Hz, 2 times 4 minutes) using 5 mm stainless steel beads (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: Total mRNA from 2 to 4 organoids were isolated using the RNeasy mini kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... 15 mM DTT) and then treated with 2 μL of 4 mg/mL Protease (QIAGEN) by incubation at 55°C for 6 hours ...
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bioRxiv - Systems Biology 2022Quote: ... 2 mL cultures were immediately added to centrifuge tubes containing 4 mL RNAprotect Bacteria Reagent (Qiagen), vortexed for 5 seconds and incubated at room temperature for 5 min ...
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bioRxiv - Genomics 2021Quote: cfDNA was extracted from 2-4 mL of plasma using the QIAsymphony liquid handling robot (Qiagen). cfDNA concentration was determined using Qubit double-strand molecular probes kit (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... rotated at 4°C for 1.5-2 hr in 0.75 mL equilibrated Ni-NTA Agarose (Qiagen), followed by packing pre-equilibrated Poly-Prep Chromatography Columns (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: DE genes between clusters 2 and 4 were fed to Ingenuity Pathway Analysis software from Qiagen. Enrichment pathways were sourced from Ingenuity core enrichment pathways.
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from about 2-4×106 cells using the RNeasy Mini Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was incubated for 2 h at 4°C while rotating with Glutathione superflow beads (Qiagen) for GST-tagged CMK-1 variants or nickel-nitrilotriacetic acid beads (Ni-NTA ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from muscle or whole animal using phenol-chloroform-isoamyl alcohol extraction or MagAttract HMW DNA Kit (Qiagen), and further purified using NucleoBond columns (Machery Nagel) ...
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bioRxiv - Genetics 2023Quote: All cDNA samples were pooled into two groups corresponding to cases (alcohol drinkers) and controls (non-drinkers) and then assayed using the Serum/Plasma Focus PCR panel (Qiagen). This commercially available microarray platform contains 179 LNA miRNA primer sets of miRNAs that have been commonly found in human plasma ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was mixed with an equal volume of chloroform/isoamyl alcohol (24:1) and separated with a phase-lock tube (Qiagen) by centrifugation (14000 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... Yeast DNA was first extracted with Phenol-Chloroform-Isoamyl alcohol before precipitating with isopropanol and resuspending in elution buffer (EB; Qiagen). YAC containing yeast were assessed by PCR using Q5® Hot Start High-Fidelity 2X Master Mix (M0494S) ...
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Phenogenomic resources immortalized in a panel of wild-derived strains of five species of house micebioRxiv - Evolutionary Biology 2023Quote: Genomic DNA was isolated from frozen (−80 °C) or alcohol-preserved muscle or spleen tissues using DNeasy Blood & Tissue Kits (Qiagen). High-quality DNA aliquots were next-generation sequenced at the Edinburgh Genomics facility using HiSeq X technology ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was purified from both IP samples and input using phenol-chloroform-isoamyl alcohol extraction followed by QIAquick PCR purification kit (Qiagen). N=2 biological repeats were performed per condition ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 ml of phenol:chloroform:isoamyl alcohol (25:24:1) was added and samples were bead beaten using a TissueLyser LT (Qiagen, Hilden, Germany) for 10 minutes at 25 Hz ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was incubated for ~2 h rotating at 4°C with Ni-NTA Resin (Qiagen, 1018244) that had been washed with wash buffer (20mM Tris HCl pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... The filtered supernatant was loaded onto 2 gravity columns each with 4 mL Ni-NTA agarose (Qiagen). The resin was pre-equilibrated with the lysis buffer ...
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bioRxiv - Genomics 2022Quote: ... cfDNA was extracted from 2 to 4 ml of plasma using the QIAsymphony liquid handling robot (Qiagen). cfDNA concentration was determined using Qubit double-strand molecular probes kit (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... 2 or 4 days and genomic DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen). Bisulfite converted DNA libraries were prepared using the Accel-NGS Methyl-Seq DNA library kit (SWIFT BIOSCIENCES) ...
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bioRxiv - Microbiology 2021Quote: ... The pooled RNA was precipitated by isopropyl alcohol with addition of glycogen followed by additional clarification with RNeasy MinElute Cleanup kit (Qiagen, Germany). NEBNext rRNA Depletion kit (NEB ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted once with 350 μL Phenol:Chloroform:Isoamyl Alcohol (25:24:1) in a phase-lock tube (Qiagen cat. no. 129046) and centrifuged 5 min at 16,000xg followed by extraction with 350 μL chloroform ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted with 1 volume of 25:24:1 phenol-chloroform-isoamyl alcohol and purified with QIAquick PCR Purification Kit (QIAGEN 28104). Libraries were prepared with the Ovation Ultralow V2 DNA-Seq Library Preparation Kit (NuGEN (Redwood City ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μl of diluted cDNA template was mixed with 4 μl QuantiTect SYBR Green PCR Master Mix (Qiagen), 100 nM forward primers (see Table 1 for sequences ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% CA630) and rotated at 4°C for 2 hours before addition of His-beads (Ni-NTA, QIAGEN). After continuous rotating for another 2 hours ...
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bioRxiv - Biophysics 2024Quote: ... for 4 hours at 37°C and purified from a 2% agarose gel (QIAquick Gel Extraction Kit, Qiagen). At this stage ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was the incubated at 4 °C with 2 ml of washed Ni-NTA agarose beads (Qiagen) for 1 hour ...
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bioRxiv - Genomics 2021Quote: ... Amino acid sequences were aligned using CLC Genomics Workbench 20.0.04 (QIAGEN, Aarhus, Denmark).
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bioRxiv - Microbiology 2024Quote: ... followed by a single extraction with an equal volume of chloroform-isoamyl alcohol (24:1) using Qiagen Maxtract High Density medium (Qiagen, Hilden, Germany). NaCl was added to 300 mM and DNA was precipitated with 2.5 volumes of 100% EtOH at −20°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was isolated at 6 time points (0, 0.5, 1, 2, 4, and 8 hours) using the RNeasy Mini kit (Qiagen) according to the manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Those included UniSpike 2 and 4 from the QIAGEN RNA Spike-In Kit (Cat. No.: 339390, QIAGEN, Hilden, Germany), which were used to monitor RNA isolation efficacy ...
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bioRxiv - Microbiology 2022Quote: ... Amino acid locations were confirmed through sequence alignment using MacVector and CLC Workbench (QIAGEN).
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bioRxiv - Immunology 2021Quote: ... The pellets were resuspended with OMV buffer and re-pelleted again by centrifugation at 200,000 g for 2 h at 4°C and lysed with Qiazol followed by RNA isolation with the miRNeasy kit (Qiagen) to obtain total RNA including the small RNA fraction ...