Labshake search
Citations for Qiagen :
1 - 50 of 1399 citations for 2 p sec Butylphenyl propionic Acid d3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 1µg/ml Aprotinin, 1mM PMSF) using 5mm Stainless Beads and TissueLyser II (frequency: 30/sec, duration 2 × 30 sec) (Qiagen, Hilden, Germany). IL-23 was measured using either a mouse IL-23 ELISA (R&D ...
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AMPK is elevated in human cachectic muscle and prevents cancer-induced metabolic dysfunction in micebioRxiv - Molecular Biology 2022Quote: ... the muscles were homogenized (2 × 30 sec, 30 Hz, TissueLyser II bead mill, Qiagen, USA) in a modified GSK3-buffer (10% glycerol ...
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bioRxiv - Cell Biology 2020Quote: All muscles were homogenized 2 × 30 sec at 30 Hz using a Tissuelyser II (Qiagen, USA) in ice-cold homogenization buffer (10% (v/v ...
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bioRxiv - Microbiology 2023Quote: TG was quantified in snap-frozen liver tissue stored at −80 □C until cryo-grinding in liquid nitrogen and 50 ±5 mg tissue added 0.9 mL of a 2:1 chloroform:methanol solution and homogenized 1 min at 50 os/sec using a TissueLyser LT (Qiagen) with beads ...
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bioRxiv - Neuroscience 2022Quote: ... and 15μL of 100ng/mL d3-CBD (Qiagen TissueLyser II (30 hz, 9 min)) ...
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bioRxiv - Genetics 2021Quote: ... and end-point PCRs were performed following 34 cycles (94 C 30 sec, 58 C 30 sec, 72 C 1 min) using the HotStarTaq Plus DNA polymerase (Qiagen, Canada) according to manufacturer’s protocol.
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bioRxiv - Genomics 2023Quote: ... Obtained lists with significantly changed genes (p<0.05, fold-change ≥2) were used in Ingenuity Pathway Analysis (IPA, Qiagen).
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bioRxiv - Neuroscience 2022Quote: ... Pathway analysis was performed for genes 2-fold up- or down-regulated with p-value < 0.05 using Ingenuity Pathway Analysis (IPA) software (Qiagen, USA).
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bioRxiv - Genomics 2020Quote: ... Realtime-qPCR was performed on 11 differentially expressed (DE) miRNAs based on miRNA-seq results (|FC| ≥ 2, P < 0.05) using miScript SYBR Green PCR Kit (Qiagen 218073, California, USA) according to the manufacturer’s instructions with StepOne Applied Biosystems real-time PCR machine (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... using a pestle and micro-tube homogenizer (for 30 sec) or TissueLyser LT (QIAGEN, Dusseldorf ...
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bioRxiv - Genomics 2021Quote: ... cfDNA was isolated from 2 ml of plasma using either the manual QIAamp circulating nucleic acid kit (Qiagen), or the semi-automated QIAsymphony DSP Circulating DNA Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... during two rounds of 30 sec at 5,000 rpm in a TissueLyser II grinder (Qiagen). Total RNA was extracted using the NucleoSpin 96 kit (Macherey-Nagel ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged at 8500×g for 30 sec and washed again with Buffer AW2 (Qiagen, 80204), and then centrifuged at full speed for 2 min ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Mosquito bodies and legs/wings were homogenized for 30 sec with TissueLyser (QIAGEN, Hilden, Germany) at 24 cycles/sec ...
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bioRxiv - Cancer Biology 2024Quote: ... and homogenized using a tissue homogenizer (frequency: 25, time: 30 sec) (TissueLyser II, Qiagen, Germany). RNA was isolated using a RNeasy mini kit (Qiagen ...
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bioRxiv - Biophysics 2021Quote: cfDNA was isolated from 2 mL of plasma for each sample via the QIAamp Circulating Nucleic Acid kit (Qiagen). ddPCR was performed using the QX200 Droplet Digital PCR System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... cfDNA was isolated from 2 mL of serum using a QIAamp Circulating Nucleic Acid Extraction Kit (Qiagen, Hilden, Germany). The DNA was eluted twice through a column for purification ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from the anthers of P and ‘CMS[P]’ with an RNeasy Plant Mini Kit (QIAGEN, Hilden, Germany). RNA was treated with RNase-free DNase (QIAGEN ...
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bioRxiv - Immunology 2024Quote: ... Nickel nitrilotriacetic acid (Qiagen) agarose beads were washed in TBS and added to filtered supernatant at a ratio of 2-3 mL resin/L and incubated on a rotator over-night at 4°C ...
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bioRxiv - Genomics 2020Quote: ... SARS-CoV-2 nucleic acids were isolated from 300 µl viral transport media using the QIAamp Viral RNA Mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... the supernatant was added to 2 ml of pre-equilibrated Nickel-nitrilotriacetic acid (Ni-NTA) agarose (QIAGEN, Cat. No. 30210) 50% slurry and rotated at 4°C for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble extracts were applied to 2-ml columns of nickel-nitrilotriacetic acid- agarose (Ni-NTA) (QIAGEN catalog no. 30210) that had been equilibrated with lysis buffer without protease inhibitors ...
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bioRxiv - Neuroscience 2021Quote: Fluorophore TYE665 labeled locked nuclear acid (LNA) (C4G2)6 and (CCG)8 probes (Supplemental table 2) were synthesized by Qiagen. The FISH protocol was adapted from (DeJesus-Hernandez et al ...
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bioRxiv - Biophysics 2023Quote: ... The soluble extracts were applied to 2 ml columns of nickel-nitrilotriacetic acid agarose (Ni-NTA) (QIAGEN catalogue no. 30210) that had been equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2024Quote: ... The filtrate was transferred to ultra-clean 2 mL tubes and 280 µL were collected for nucleic acid extraction using the QIAamp Viral RNA mini kit (Qiagen). The extract was eluted in a final volume of 40 µL and stored at -80 °C.
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bioRxiv - Immunology 2020Quote: ... Disruption of microbial cells was enhanced using TissueLyser II (QIAGEN, 30 shakes/sec., 1 min., 2x). To lyse Gram-negative bacteria ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant containing solubilized protein was filtered using a 0.22 μM nylon membrane filter and incubated with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, Germantown, MD) at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Nucleic acid extraction was performed using EZ1&2™ Virus Mini Kit v2.0 on platform EZ1 Advanced XL (Qiagen, Heidelberg, Germany) and tested for a panel of respiratory viruses as previously reported (Piralla et al. ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from each SEC fraction by miRNeasy serum/plasma kit (Qiagen 1071073 Lot#160020206) after adding 1.0 x 10^8 copies/μL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035) ...
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bioRxiv - Immunology 2020Quote: ... Mechanical disruption of microbial cells was done using TissueLyser II (QIAGEN, 30 shakes/sec., 10-20 min.) and the samples were centrifuged (10,000 g ...
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bioRxiv - Molecular Biology 2022Quote: ... for 1 min 30 sec at 30 Hz before RNA extraction using the RNeasy micro kit (Qiagen). RNA was sequenced using the DNBseq platform at the Beijing Genomics Institute (BGI Group ...
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bioRxiv - Plant Biology 2023Quote: ... twice for 30 sec at 30 Hz before RNA extraction using the RNeasy Plant Mini kit (Qiagen). For RT-qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were homogenized for five 3-minute cycles of 30 shakes/sec using a TissueLyser II (Qiagen) and transferred to an RNase free microfuge tube (Ambion ...
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bioRxiv - Microbiology 2020Quote: We evaluated the impact of using Nanotrap particles to capture and concentrate SARS-CoV-2 on several nucleic acid extraction methods: the QIAamp® Viral RNA Mini Kit from QIAGEN (52906); the RNeasy® Mini Kit from QIAGEN (74106) ...
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bioRxiv - Neuroscience 2024Quote: ... Locked nucleic acid (LNA) probes (Qiagen) to detect either sense (5’ TYE563-labelled CCCCGGCCCCGGCCCC ...
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bioRxiv - Immunology 2024Quote: ... RNA extraction was performed by QIAGEN Nucleic Acid Isolation Service (QIAGEN, Germany). RNA integrity number (RIN ...
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bioRxiv - Cell Biology 2020Quote: ... Circulating cell-free nucleic acid (ccfNA) was extracted using the Qiamp Circulating Nucleic Acid kit (Qiagen, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Oral fluid samples were prepared by homogenization of 200 μl sample (30 sec at 15 Hz) in a Tissuelyser II (Qiagen) followed by centrifugation (2 min at 10.000 rpm) ...
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bioRxiv - Microbiology 2021Quote: ... was added to each tube and samples were homogenised twice at 25 Hz for 30 sec using a TissueLyser II (85300; QIAGEN) and centrifuged for 2 min at 2,000 x g ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... 45 cycles at 95° for 15 sec and 60° for 1 min were performed in the Rotor-Gene Q real-time PCR cycler (Qiagen). The numbers of copies of the RdRP gene in the samples were calculated using an RdRP cDNA standard curve.
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... at 6 m/s for 50 sec and total RNA extracted with the RNeasy Lipid Tissue Mini Kit (#74804; Qiagen). For SCP (D18 ...
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bioRxiv - Microbiology 2024Quote: ... were used to swab the footpad skin extensively for at least 30 sec and collected in PowerBead Pro Tubes (Qiagen). DNA was isolated using the DNeasy PowerSoil Pro kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... ICAM and P-Selectin were from the QuantiTect primer assay kit (Qiagen). Results are expressed following normalization using 18S RNA.
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bioRxiv - Immunology 2021Quote: ... Ni-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) was used to purify the protein via gravity flow and proteins were eluted as previously described37,38 ...
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bioRxiv - Microbiology 2021Quote: ... and associated nucleic acid extraction (Qiagen, Germany) kits were used ...
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bioRxiv - Genomics 2024Quote: ... and QIAamp Circulating Nucleic Acid Kit (Qiagen) for EAC samples ...
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bioRxiv - Pathology 2024Quote: ... the Circulating Nucleic Acid Kit (Qiagen, 55114) was employed with specific modifications to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Ni-nitrilotriacetic acid (NTA) beads (Qiagen, USA) were used to purify the protein ...
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bioRxiv - Microbiology 2020Quote: ... whereas 30 mg of lung tissue was homogenized in 600 μl RLT-buffer containing β–mercaptoethanol for 30 sec at 15 Hz using the TissueLyser II (Qiagen).
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bioRxiv - Cell Biology 2021Quote: ... Differentially expressed genes (P<0.01) were analysed with Ingenuity Pathway Analysis software (Qiagen) applying the Upstream Regulator Analysis approach ...