Labshake search
Citations for Qiagen :
151 - 200 of 10000+ citations for 2'3' cyclic GAMP cGAMP ELISA Kit 384 well Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2023Quote: ... Each well in the NAB plate was then washed once with 500 μL buffer AW1 (QIAGEN) and once with 500 μL of buffer AW2 (QIAGEN) ...
-
bioRxiv - Microbiology 2021Quote: ... or the DNeasy® 96 Blood & Tissue plate kit (Qiagen) according to supplier’s protocol.
-
bioRxiv - Immunology 2021Quote: ... and a custom 384-gene array (RT2 Profiler PCR array; Qiagen) focused on the inflammatory response/autoimmunity was performed following the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2020Quote: ... we carried out initial crystallization trials in 15-well hanging drop format using EasyXtal crystallization plates (Qiagen) and a crystallization screen that was designed to explore the chemical space around the crystallization conditions reported by Blomberg et al.15 Crystallization drops were prepared by mixing 1 μL of protein solution with 1 μL of the mother liquor ...
-
bioRxiv - Cell Biology 2021Quote: ... SF9 cells at 1×106 SF9 cells/ml were grown in 24 well round bottom plates (Qiagen) and infected with baculovirus of the indicated single subunits ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were transfected in 10 cm2 or 6-well plates at ∼60% confluency using PolyFect (Qiagen 301107) and washed with complete media 6 h later.
-
bioRxiv - Molecular Biology 2020Quote: SH-SY5Y cells plated in 6-well plates were lysed using 750ul of QIAzol Lysis reagent (Qiagen) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... cells were stained as above and index-sorted directly into 96 well plates containing Buffer TCL (Qiagen) supplemented with 1% β-mercaptoethanol using a BD FACS Aria II ...
-
bioRxiv - Neuroscience 2020Quote: ... the 15 mm diameter coverslips were placed in 12 well plate with 600μl of Buffer RLT (Qiagen). These plates were stored at −80 °C until RNA extraction ...
-
bioRxiv - Microbiology 2020Quote: ... The extraction tubes were then agitated twice in a 96-well plate using Tissue lyser II (Qiagen) at 30 Hz/s for 5 min.
-
bioRxiv - Biochemistry 2023Quote: ... we carried out initial crystallization trials in 15-well hanging drop format using EasyXtal crystallization plates (Qiagen) and a crystallization screen that was designed to explore the chemical space around the crystallization conditions reported by Broom et al.14 Crystallization drops were prepared by mixing 1 μL of protein solution with 1 μL of the mother liquor and sealing the drop inside a reservoir containing an additional 500 μL of the mother liquor solution ...
-
bioRxiv - Biochemistry 2023Quote: ... The RT-quantitative PCRs were carried out in four biological replicates in a 96-well plate (QIAGEN) using a QIAcuity system (QIAGEN) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Live single cells were sorted into 96 wells plates containing 10µl of TCL lysis buffer (Qiagen 1031576), supplemented with 1% β-mercaptoethanol.
-
bioRxiv - Cell Biology 2021Quote: ... per well and further purified using an RNAeasy cleanup kit (Qiagen). An equal amount of RNA for each sample was converted to cDNA using PrimescriptTM RT Reagent Kit (Takara Bio) ...
-
bioRxiv - Microbiology 2019Quote: ... using the 96-well-format MoBio PowerSoil Kit (MoBio Laboratories; Qiagen) following the manufacturer’s instruction ...
-
bioRxiv - Systems Biology 2021Quote: ... Samples were then eluted into a clean 96DW plate from each well using 110μL of Buffer AE (QIAGEN) preheated to 56°C ...
-
bioRxiv - Microbiology 2020Quote: A 6.5 nM concentration of His-tagged 16055 SOSIP.v8.3 trimer in TBS was added to a 96-well Ni-NTA plate (Qiagen) for 2 h at RT ...
-
bioRxiv - Pathology 2021Quote: Pre-spotted custom plates (96-well format) were designed to comprehend 89 different miRCURY LNA miRNA primers (Qiagen), three assays for small nuclear or nucleolar RNAs as reference candidates (SNORD44 ...
-
bioRxiv - Systems Biology 2022Quote: ... siRNA was transfected into RWPE-1 cells in a 24-well plate using HiPerFect Transfection Reagent (Qiagen, 301704). siRNA ...
-
bioRxiv - Immunology 2022Quote: ... 4h and 20h post activation 2000 cells were sorted directly into 0.2ml semi-skirted 96 well PCR plate (Peqlab) containing 10μl 1x TCL Buffer (Qiagen) and 1%(v v-1 ...
-
bioRxiv - Microbiology 2022Quote: ... Genomic DNA extraction was performed on 384 isolates using Qiagen DNeasy Blood and Tissue Kit (DNeasy® Blood & Tissue Handbook, Qiagen, Hilden, Germany, 2020) followed by quantification of double stranded DNA product using Quantit dsDNA Assay Kit Broad range and Nanodrop (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2022Quote: ... Genomic DNA extraction was performed on 384 isolates using Qiagen DNeasy Blood and Tissue Kit (DNeasy® Blood & Tissue Handbook, Qiagen, Hilden, Germany, 2020) followed by quantification of double stranded DNA product using Quantit dsDNA Assay Kit Broad range and Nanodrop (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... duplicate wells combined and RNA extracted using RNeasy Extraction kit (Qiagen, UK). RNA was converted to cDNA using the iScript cDNA synthesis kit (BioRad ...
-
bioRxiv - Bioengineering 2023Quote: RNA extraction was performed on cells from individual wells of a 24-well tissue-culture dish at each differentiation time-point using an RNEasy mini kit (Qiagen 74104). cDNA was generated from RNA using an Applied Biosystems High Capacity cDNA Reverse Transcription kit (ThermoFisher 4368814) ...
-
bioRxiv - Microbiology 2021Quote: ... After 24 hours the media was removed for ELISA and the cells were lysed and RNA extracted (RNeasy Mini Kit, Qiagen).
-
bioRxiv - Molecular Biology 2022Quote: ... 10,000 cells per sample were sorted into a 96 well plate containing 50 μl RLT plus Lysis Buffer (Qiagen) supplemented with 1% 2-mercaptoethanol and frozen at −80 °C immediately ...
-
bioRxiv - Molecular Biology 2019Quote: Cells seeded in 24-well plates were transfected with siRNA against intended targets (Qiagen, sequences provided in Table S3) using Lipofectamine RNAiMAX (Thermo Fisher ...
-
bioRxiv - Systems Biology 2020Quote: ... Samples were then eluted into a clean 96DW plate from each well using 110 μL of Buffer AE (QIAGEN) preheated to 56°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were plated in 12 well plates at a dilution of 1.5×106 and were transfected with the indicated constructs using Effectene (Qiagen) according to manufacturer’s protocols ...
-
bioRxiv - Molecular Biology 2022Quote: 2.0 × 105 HeLa cells were transfected in 12 well plates with control (NTC: all-stars non-targeting control, Qiagen) or DDX39B-targeting (D11 ...
-
Deconstructing the role of iron and heme in the structure of healthy human gut microbial communitiesbioRxiv - Microbiology 2022Quote: ... Two hundred fifty microliters of each sample were transferred into 96-well PowerBead Plates (Qiagen, 27500-4-EP-BP). Seven hundred fifty microliters of RLT buffer from an AllPrep DNA/RNA 96 Kit (Qiagen ...
-
bioRxiv - Plant Biology 2022Quote: ... Sections were immediately placed into 96-well plates (on ice) containing 10 μL of RLT plus (Qiagen, Hilden, Germany). All instruments and surfaces were cleaned with 80% ethanol ...
-
bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from hESCs or NPCs grown in a 6-well plate (9.5 cm2) using the RNeasy minikit (Qiagen). A DNase 1 digestion step was performed during RNA extraction to degrade remaining genomic DNA ...
-
bioRxiv - Immunology 2022Quote: ... Single cells from three to four mice per each condition were index-sorted directly into 96-well plates containing 5μl of TCL buffer (Qiagen) supplemented with 1%β-mercaptoethanol using a BD FACS Aria II instrument ...
-
bioRxiv - Molecular Biology 2019Quote: 1×106 MIA PaCa-2 or HAP1 cells were seeded per 6 well plate and co-transfected with 0.5 µg pCas-Guide plasmid using Effectene transfection reagent (Qiagen). 24 hrs after transfection ...
-
bioRxiv - Immunology 2021Quote: ... viable in vitro differentiated CD4+ T cells were sorted into 96-well plates containing 5 μl TCL Buffer (QIAGEN) with 1% 2-mercaptoethanol ...
-
bioRxiv - Immunology 2022Quote: His-tagged RBD of SARS-CoV S was diluted to a concentration of 0.8 μg/mL in Tris-buffered saline (TBS) and immobilized on NiNTA 96-well plates (Qiagen) for 2h at RT ...
-
bioRxiv - Systems Biology 2023Quote: ... Samples were then eluted into a clean 96DW plate from each well using 110 μL of buffer AE (QIAGEN) preheated to 56 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded in 24-well plates and transfected with the desired plasmids using Effectene Transfection Reagent (#301427, QIAGEN) following the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2023Quote: ... total RNA was isolated from Hep3B cell spheroids on the pillar plate and in the ULA plate using a RNeasy Plus kit (Qiagen,74134). One μg of RNA was converted into cDNA using the high-capacity cDNA reverse transcription kit according to the manufacturer instructions (Applied Biosystems ...
-
bioRxiv - Microbiology 2020Quote: ... or in 96-well format with a QIAprep 96 Turbo miniprep kit (Qiagen). We performed 98°C BarSeq PCR protocol as described previously [63 ...
-
bioRxiv - Genetics 2021Quote: ... DNA was extracted using Qiagen 96-well DNeasy kits (Qiagen P/N 69581). Sequencing libraries were prepared using the Illumina Nextera Kit and custom barcoded adapter sequences ...
-
bioRxiv - Microbiology 2023Quote: ... DNA was extracted from each well using the QIAamp DNA Mini kit (Qiagen) after five freeze-thaw cycles in liquid-nitrogen and 56°C water-bath ...
-
bioRxiv - Immunology 2020Quote: ... A total of 78 serum samples (positive for HEV IgM in ELISA) were subjected to RNA extraction using QIAamp Viral RNA Mini Kit (Qiagen, Germany). Using SuperScript™ III One-Step RT-PCR System with Platinum™ Taq (Invitrogen ...
-
bioRxiv - Genomics 2019Quote: ... We used a 100 µm nozzle to sort single cells into 96-well plates containing 5 µl TCL buffer (Qiagen) with 1% beta-mercaptoethanol for Smart-seq2 and 384-well plates containing 0.6 µl 1% NP40 (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2021Quote: ... Crystal screening was performed with a Gryphon robot (Art Robbins instruments, Sunnyvale, USA) using sitting-drop 96-well plates and Screening Suites (Qiagen). Optimization experiments were set up in 24-well hanging drop crystallization plates ...
-
bioRxiv - Cancer Biology 2020Quote: ... Our panel of 88 target genes was prepared on a 96-well plate format with housekeeping genes and controls (RT2 PCR array, Qiagen). Expression was quantified on an ABI7500 real-time PCR machine (Applied Biosystems ...
-
bioRxiv - Immunology 2020Quote: Single cells were sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, Qiagen 1031576) supplemented with 1% β-mercaptoethanol ...
-
bioRxiv - Genetics 2019Quote: ... S2 and KC cell lines were stably transduced with the pMKInt-Hyg vector encoding RasV12Common and RasV12Rare cDNAs using 1000 ng of DNA in 6 well plates as instructed by Effectene transfection reagent (Qiagen). The following day ...
-
bioRxiv - Immunology 2021Quote: ... single MR1-tetramer-binding cells from Peruvian participant 7-3 and blood bank donors 702A and 703A were sorted into 96-well plate coated with Vapor-Lock (Qiagen) containing Iscript cDNA synthesis mixture (Bio-Rad ...