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Citations for Qiagen :
401 - 450 of 2263 citations for Polyhomeotic Like Protein 1 PHC1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Poly-histidine-tagged recombinant proteins were purified using Ni-NTA-Agarose (Qiagen, Hilden, Germany) beads according to manufacturer’s instructions and were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were digested with 30 μL of Proteinase K II (QIAGEN, GmbH, Hilden, Germany). DNA was washed with AW1 and AW2 buffers (QIAGEN ...
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bioRxiv - Biochemistry 2020Quote: ... The target proteins were purified by affinity chromatography using a Ni-NTA column (Qiagen) with the lysis buffer supplemented with 20 mM and 200 mM imidazole serving as the washing buffer and elution buffer ...
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bioRxiv - Bioengineering 2021Quote: ... The soluble hEKL proteins were purified using a Ni-NTA agarose resin (Qiagen, Germany) and subsequently HiLoad 16/60 superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... The soluble protein fraction was then purified with pre-equilibrated Ni-NTA resin (Qiagen), recovered in elution buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was purified by affinity chromatography using Ni-NTA agarose (Qiagen, Hilden, Germany), followed by gel filtration chromatography ...
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bioRxiv - Cell Biology 2021Quote: ... We purified the 6xHIS fusion protein on nickel-nitrilotriacetic acid agarose (Qiagen, Valencia, CA) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM 2-mercaptoethanol) and the proteins eluted from nickel-NTA agarose beads (Qiagen; using buffer containing 25 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant HisCheY or HisCheY* and bound proteins were purified using nickel agarose columns (Qiagen) under native conditions per manufacturers’ instructions.
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bioRxiv - Biochemistry 2020Quote: ... and proteins were purified by IMAC using Ni-NTA spin columns (QIAGEN, Hilden, Germany). Proteins were eluted in 100 μL elution buffer (50 mM sodium phosphate pH 8.0 ...
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bioRxiv - Bioengineering 2020Quote: ... and recombinant proteins were isolated from the periplasmic fraction using Ni-NTA beads (Qiagen). Following washing and subsequent elution with 50 mM Tris (pH 8) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Uncleaved protein with 6xHisTag were isolated from the lysate using Ni-NTA resin (Qiagen). The flow-through (FT ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were incubated with Nickel-nitrilotriacetic acid (NTA) sepharose (Qiagen, Hilden, Germany) at 4°C with slight shaking for 1 h ...
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bioRxiv - Plant Biology 2020Quote: ... His-βC1 fusion proteins were purified using Ni-nitrilotriacetate (Ni-NTA) agarose (Qiagen, 30210) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... His-tagged proteins from the supernatant were incubated overnight with Ni-NTA agarose (Qiagen) at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and associated protein network by using Ingenuity® Pathway Analysis (IPA) software (QIAGEN Inc.), relying on IPA’s proprietary algorithm to evaluate and minimize sample source bias ...
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bioRxiv - Immunology 2021Quote: ... and recombinant proteins produced in the supernatant were purified using Ni-NTA agarose (QIAGEN). To biotinylate RBD proteins ...
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bioRxiv - Physiology 2021Quote: ... Total RNA was extracted from EVs (20 μg protein) using miRNeasy Mini Kit (Qiagen). RNA was then reverse-transcribed using TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were purified by affinity chromatography using a Ni-NTA agarose resin (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... The secreted protein in the culture supernatant was purified using Ni-NTA agarose (Qiagen) according to the manufacturer’s protocol and dialyzed against PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were equalized for protein concentration and incubated with NiNTA agarose beads (Qiagen, #L30210) for O/N ...
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bioRxiv - Microbiology 2021Quote: ... Protein was purified using Ni-NTA agarose beads according to the manufacturer’s instructions (Qiagen). A buffer exchange was performed by centrifugation of the protein preparation through an Amicon Ultra-15 centrifugal filter unit (Millipore ...
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bioRxiv - Bioengineering 2020Quote: ... The enriched proteins were searched again Ingenuity pathway analysis (QIAGEN, Redwood City, CA, USA) for their primary subcellular location and enriched molecular functions ...
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bioRxiv - Neuroscience 2023Quote: ... significantly differentially expressed proteins from all translatomes were analysed for IPA pathway analysis (Qiagen) to highlight activated/inhibited pathways when comparing control (NBH ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were eluted using Ni-NTA spin columns according to the manufacturer’s protocol (Qiagen). GST-α synuclein fusion protein tagged at the N-terminus was obtained from Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified according to manufacturer’s instructions by Ni2+-NTA agarose beads (QIAGEN). Washing steps were performed with a buffer containing 20 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: Total cellular RNA was extracted using AllPrep DNA/RNA/Protein mini kit (Qiagen, UK). Reverse transcription was carried out using kits from Invitrogen following the manufacturer’s instructions (SuperScript First-Strand Synthesis System for RT-PCR) ...
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bioRxiv - Immunology 2024Quote: ... Proteins were purified from filtered supernatants using nickel nitriloacetic acid (Ni-NTA) agarose (QIAGEN) via gravity flow ...
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bioRxiv - Microbiology 2024Quote: ... Soluble protein fractions were then mixed with pre-equilibrated Ni-NTA agarose resin (Qiagen) and incubated at 4°C for 1 h with rotation ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria isolates then underwent DNA isolation using AllPrep Bacterial DNA/RNA/Protein Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins harboring the His6 tag were captured by incubation with Ni- NTA resin (Qiagen) (2ml resin per bacterial lysate from 1L culture ...
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bioRxiv - Genetics 2023Quote: Total protein was extracted from snap frozen tissues with a Tissue Lyser II (Qiagen) using 5mm Bead Beater balls (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was then isolated with Qiagen AllPrep DNA/RNA/Protein Mini Kit (Qiagen # 80004). Breast microstructures from 5 independent donors were exposed to vehicle (PBS and PBS plus DMSO) ...
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bioRxiv - Microbiology 2023Quote: ... The remaining protein mixtures were incubated with 50 μL Ni-NTA agarose beads (QIAGEN) at 4℃ for 1.5 h with constant rotation ...
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bioRxiv - Microbiology 2023Quote: ... the proteins were purified by affinity chromatography with Ni-NTA superflow affinity column (Qiagen) as previously described (33).
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bioRxiv - Cell Biology 2022Quote: Constructs for recombinant bacterial protein expression were all cloned in the pQE plasmid (Qiagen). Su9-DHFR and CaM-3C-Alfa-Sec61β(2–60)-OMP25(112–145)F128Amber were cloned downstream of a His14-bdSUMO tag ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged proteins were purified by affinity chromatography on Ni-NTA Agarose resin (Qiagen) and eluted with buffer containing 0.5 M imidazole ...
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bioRxiv - Neuroscience 2023Quote: ... and day 7,14,21 and 28 of maturation using AllPrep DNA/RNA/Protein (80004, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The DENV2 EDIII and Halo-tag proteins were purified using Ni-NTA agarose (Qiagen) from the cell culture medium ...
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bioRxiv - Immunology 2024Quote: ... The recombinant proteins with His-tag were purified with Ni-NTA Agarose (30210, Qiagen). The proteins loaded onto the Ni-NTA column were washed with 60 column volumes of the Buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNA was extracted using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Cat. #80004) and stored at -80°C until use ...
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bioRxiv - Molecular Biology 2024Quote: Human CRM1 was expressed as His-tag fusion protein from a pQE60 plasmid (Qiagen) in E ...
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bioRxiv - Molecular Biology 2024Quote: ... Recombinant protein was captured by overnight incubation with 3 ml Ni-NTA resin (Qiagen) followed by washing with 30 ml denaturing buffer (8 M urea ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein was incubated with 100 μl of Ni-NTA agarose beads (QIAGEN) (washed and preincubated with 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein in the soluble lysate was bound to 8 mL Ni-NTA resin (Qiagen), washed with 10 mL low salt wash buffer ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were purified were purified with the Strep-tactin Superflow Plus resin (Qiagen) and eluted by cleavage with desthiobiotin ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His is a peroxidase-conjugated antibody (Qiagen). The membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... or mouse anti-RGS-His antibody (Qiagen; cat # 34610) at 1:5000 dilution followed by goat anti-rabbit-HRP conjugate (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-penta-His antibody (34660; Lot# 136244018; Qiagen); mouse anti-Myc tag mAb ...
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bioRxiv - Microbiology 2020Quote: ... The anti-His antibody conjugated to horseradish peroxidase (Qiagen) was used at a dilution of 1:5,000 ...