Labshake search
Citations for Qiagen :
401 - 450 of 10000+ citations for Mouse B And T Lymphocyte Attenuator BTLA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: DNA was isolated from P14 tail biopsies using the Gentra Puregene Mouse Tail Kit according to the manufacturer’s instructions (Qiagen, Valencia, CA). Approximately 250 ng of DNA was digested with BAMH1-HF (R3136 ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from dissected CD31+ BECs from the mouse brain using an RNA extraction kit (#74104, Qiagen, MD, USA). The extracted RNA was stored in RNAse-free water at -80°C until transfer to the Boston University School of Medicine Microarray Core Facility ...
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bioRxiv - Immunology 2020Quote: ... was extracted from two and ten week mouse heart samples stored in RNA Later with the RNeasy fibrous tissue kit (74704, Qiagen, UK). RNA was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: DNA was isolated from P14 tail biopsies using the Gentra Puregene Mouse Tail Kit according to the manufacturer’s instructions (Qiagen, Valencia, CA). For Gabra2 ...
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bioRxiv - Immunology 2022Quote: ... the livers were perfused 45 hr post mosquito-bite challenge with 1× PBS and total RNA was extracted from the lower right caudate process of the caudate lobe of the liver from each mouse using miRNeasy kit (Qiagen: 217004). The complementary DNA (cDNA ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from fibroblasts obtained from each individual mouse from each of the five non-CC inbred strains using AllPrep DNA/RNA Mini kit (Qiagen, #80204). The concentration and purity of extracted DNA was determined using a NanoDrop 2000 UV-vis spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... hACE2 expression or SARS-CoV-2 nucleocapsid (N) gene in individual mouse organs was determined using QuantiNova SYBR Green PCR kit (Qiagen #208052) in combination of 500 nM of hACE2 gene specific primer set (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from mouse cortices (~0.25 mg per sample) and infected neuronal cultures (5w2d post transduction) using RNeasy kits (Qiagen, 73304&73404) according to the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from patient tissue samples and flash frozen mouse xenograft tumor samples (n = 4) using the RNeasy kit (Qiagen; #74104) and converted to cDNA using the qScript cDNA Synthesis kit (Quantabio ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA from cultured mouse neuro 2a cells was extracted using AllPrep DNA/RNA/Protein Mini Kit (80004, Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... all samples were diluted to the same RNA concentration and 250 ng of RNA from each sample spiked with 100 ng mouse RNA to control reaction efficiency were reverse transcribed using the miScript II RT kit (Qiagen, 218161) with HiFlex Buffer according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from the proteinase K-digested tail tips of mouse or peripheral blood mononuclear cell (PBMC) of human donors using DNeasy blood tissue kit (Qiagen, #69506). 25 ng of genomic DNA was subjected to bisulfite conversion using a commercial kit (Zymo Research ...
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bioRxiv - Cancer Biology 2022Quote: CD4+ and CD8+ T cells were collected on the FACS Aria Fusion into RNAse free eppendorfs (Qiagen), transported on ice and cell pellets were resuspended and lysed in RLT buffer (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Samples were then centrifuged and plasma isolated and stored at -20°C prior to testing in the QuantiFERON IFNγ ELISA (Qiagen). Acceptance criteria for the assay specified by manufacturers were always met.
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Neuroscience 2022Quote: PCR with forward and reverse primers (see Table 1) was performed on mouse hypothalamic cDNA (isolated with miRNeasy Mini Kit, Qiagen, Hilden, Germany). PCR products were ligated into pGEMT.easy (Promega ...
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bioRxiv - Genomics 2020Quote: ... and AH (20 weeks) old C57/Bl6/J mouse hearts (n= 4-6/group) using the RNeasy Mini Kit (Qiagen, Cat.74106) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA and miRNA fractions were isolated from the mouse frozen tissue with the miRNeasy Micro Kit protocol (Qiagen, Toronto, ON, Canada). All RNA samples were determined to have 260/280 and 260/230 values ≥1.8 ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted and purified from 20 mg (2 pellets) of stool from each mouse using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germantown, MD). The concentration of DNA in samples was determined by spectrophotometry ...
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bioRxiv - Cancer Biology 2022Quote: Total RNAs from mouse tumors were extracted and purified using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: DNA was isolated from tail biopsies using the Gentra Puregene Mouse Tail Kit according to the manufacturer’s instructions (Qiagen, Valenica, CA, USA). Genomic DNA was digested with BamHI-HF (New England Biolabs ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNA and miRNA fractions were isolated from the mouse frozen tissue with the miRNeasy Micro Kit protocol (Qiagen, Toronto, ON, Canada). All RNA samples were determined to have 260/280 and 260/230 values ≥1.8 ...
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bioRxiv - Microbiology 2021Quote: ... Total genomic DNA was extracted from each ked lysate and blood (camel, mouse, and rabbit) using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was isolated from the tail tip of a wild-type C57BL/6J mouse using the DNEasy Blood and Tissue Kit (Qiagen, Hilden, Germany). The genomic C-terminal region of OGT was amplified using the Q5 Hot Start High Fidelity 2x master mix (New England Biolabs) ...
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bioRxiv - Immunology 2023Quote: Total cellular RNA was extracted from mouse placental tissues or HTR8 cells using a RNeasy Plus Mini Kit (Qiagen, Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNAs from mouse tumors were extracted and purified using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: Mouse liver RNA was extracted from 10 mg (± 2 mg) of preserved tissue using RNEasy kits and QIAshredders (QIAGEN; Germantown, MD, USA). Aliquots of RNA from each sample were assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Parasite genomic DNA was purified from the blood of a transfer mouse using the Qiagen QIAamp DNA Blood Kit (Qiagen, Cat# 51106), and genotyping PCR was performed to assess integration into the target locus ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from blood-free cranial lobes of the right mouse lung using the RNeasy Tissue Mini Kit (Qiagen, Hombrechtikon, Switzerland). Isolated RNA was reverse-transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics ...
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bioRxiv - Developmental Biology 2022Quote: ... SPACA6 concentrated to 7 mg mL−1 in Buffer B was mixed in a 1:1 volumetric ratio (0.3:0.3 mL) with JCSG+ (Qiagen), Cryos (Qiagen) ...
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bioRxiv - Immunology 2020Quote: DAFhi and DAFlo GC B cells (CD19+ CD20+ CD38+ IgD−) were resuspended in RLT cell lysis buffer (Qiagen) after flow cytometric sorting ...
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bioRxiv - Immunology 2023Quote: ... ∼12,000-15,000 B cells from the tetramer-enriched fraction were sorted directly into Buffer RLT (Qiagen; Hilden, DE) prior to shipment on dry ice to iRepertoire ...
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bioRxiv - Molecular Biology 2023Quote: Inhibitors (antimiRs) against miR26b-5p and miR200a/b/c-3p were purchased from Qiagen (339130 and 339160 respectively). AntimiRs in injection media (1mM Tris-HCL pH 7.5 and 0.5 mM EDTA in embryo grade water ...
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bioRxiv - Bioengineering 2023Quote: ... The RT-qPCR experiments used Student’s t-test of the experimental group compared to the sham control (Qiagen GeneGlobe RT2 Profiler PCR Data Analysis) ...
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bioRxiv - Physiology 2022Quote: ... Mouse Obesity (PAMM- 017ZC-12) array and Mouse Aging (PAMM-178ZC) from Qiagen (Maryland, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from the indicated mouse tissues and cultured cells using RNeasy® Lipid Tissue Mini Kit (Qiagen, Valencia, CA, USA). Reverse transcription reactions containing 1 μg of total RNA were performed using PrimeScript™ (Takara ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from donor cecal contents and day 0 conventionalized mouse feces using the Qiagen PowerFecal DNA Kit (Qiagen, Hilden, Germany; 12830) following the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... eyes were enucleated and retinas were immediately dissected and total RNA was extracted from mouse retinal tissue using Qiagen RNeasy Mini Kit as per manufacturers protocol (Qiagen, Valencia, CA, USA) and reverse-transcribed using iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: RNA was extracted from mouse islets (∼300 islets per sample, pooled from ∼10 mice of the same genotype) using RNAeasy Micro Kit (Qiagen, Valencia CA, USA) as per manufactures instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from the mouse cells/tissues and human cell lines using the RNeasy® Mini Kit (Qiagen Inc., Germantown, MD). For human platelets and MEG-01 cells ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and 3.5 ml of RLT buffer with B-mercaptoethanol (10 µl for every 350 µl of RLT buffer – Qiagen) was added ...
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bioRxiv - Immunology 2020Quote: ... Gene ontology analyses for high affinity/SHM class-specific GC B cells were performed with Ingenuity Pathway Analysis (Qiagen) software using avg_logFC values of all genes significantly enriched in at least one class ...
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bioRxiv - Immunology 2023Quote: ... GC B cells (Live/Dead-CD19+IgD-CD95+GL-7+) and naïve B cells (Live/Dead-CD19+IgD+) were flow sorted into RLT Plus buffer (Qiagen) following pre-enrichment with the Pan B Cell Isolation Kit II (Miltenyi) ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 1 μg of a pcDNA3.1 vector containing human αSyn cDNA (provided by T. Baron, Anses, Lyon, France) using Effecten transfection reagent (Qiagen). Positive clones were selected using geneticin and cultured in Dulbecco’s modified Eagle’s medium (DMEM/F-12 ...
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bioRxiv - Immunology 2021Quote: Between 104 and 3.104 murine and human T cells were sorted from lymph nodes and tumors in TCL buffer (Qiagen) with 1% of β-mercaptoethanol ...
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bioRxiv - Developmental Biology 2020Quote: ... the allantois was dissected out and lysed in 10 μl DirectPCR (Tail) digest reagent (Qiagen, catalog no. 102-T) supplemented with 1 μl of proteinase K overnight at 55°C ...
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bioRxiv - Immunology 2021Quote: ... viable in vitro differentiated CD4+ T cells were sorted into 96-well plates containing 5 μl TCL Buffer (QIAGEN) with 1% 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA (gDNA) was extracted from J-Lat cells 10.6 and HIV-1 negative CD4+ T cells with the QIAcube (Qiagen), using the QiaAmp DNA mini kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: CD8+ T cells from PBMCs and colon were FACS sorted directly into 50 μL of RLT Lysis Buffer (QIAGEN) supplemented with 1% v/v 2-mercaptoethanol ...
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bioRxiv - Biophysics 2023Quote: ... The α1(L9’T) and αm mutations were introduced individually or serially by site-specific mutagenesis (QuikChange II, Qiagen). Mutations βm and γm were obtained from GENEWIZTM ...