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Citations for Qiagen :
401 - 450 of 1223 citations for Mouse Anti Human Papilloma virus types 16 18 716 281 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... rRNA depletion sequencing library was prepared by using QIAGEN Fastselect rRNA 5S/16S/23S Kit (Qiagen, Hilden, Germany). Strand-specific RNA sequencing library was prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Samples were sent to Glasgow Polyomics for ribosomal depletion using a QIAseq FastSelect 5S/16S/23S Kit (Qiagen), cDNA library preparation using a TruSeq Stranded Total RNA Library Prep Gold (Illumina ...
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bioRxiv - Zoology 2021Quote: ... dead mites were pierced with an 18-gauge needle under a dissecting microscope and digested overnight in lysis buffer and proteinase K at 56 °C (Qiagen, Valencia, CA). Final DNA product from each mite was eluted in 60 µL of AE buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 20 ng of cDNA was used for qPCR with the Human Cellular Senescence and Human Aging RT2 Profiler™ PCR Arrays (Qiagen). Arrays were run on the 7300 Real Time PCR System (Life Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA from the control human TSCs as well as WWTR1-KD human TSCs were isolated using RNeasy Mini Kit (Qiagen, 74104) per the manufacturer’s protocol with on column DNase digestion ...
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bioRxiv - Molecular Biology 2021Quote: Hepatic gene expression was investigated using qPCR arrays designed for human (RT2 Profiler PCR arrays – ‘Human Fatty Liver’, Qiagen France SAS). Then ...
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bioRxiv - Immunology 2021Quote: ... the RT2 Profiler Array “Type I Interferon Response” (Cat. No. 330231 PAMM-016ZA) (Qiagen, Hilden, Germany) was used ...
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bioRxiv - Genetics 2020Quote: ... Primer design was optimised to work with the Type-it Microsatellite PCR kit (QIAGEN, Hilden, Germany).
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bioRxiv - Microbiology 2021Quote: ... The PCR mixture contained 1x master mix for the Type-it Microsatellite PCR Kit (Qiagen, France), 3 µL of Q-Solution and 0.25 µM of each primer in a total volume of 30 µL ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was isolated from wild-type and fap92 cells using RNeasy Plus Mini Kit (Qiagen). cDNA synthesis was performed using SuperScrip IV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Genetics 2019Quote: ... RNA from collected cell types and (cancer) cell lines was isolated using the miRNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Each reaction contained 5 μL DNA solution and PCR mixture with Taq Type-it (Qiagen®). PCR products were analyzed by capillarity electrophoresis on an ABl3130xl sequencer ...
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bioRxiv - Microbiology 2020Quote: ... Viral nucleic acids were then isolated using the Qiagen QIAamp MinElute Virus Spin Kit without the use of AW1 buffer or carrier RNA (Qiagen, Valencia, CA, USA). Random hexamers were used to prime cDNA synthesis (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in 384 well plates on an Applied Biosystems 7900HT Fast Real-Time PCR system using the QuantiTect Virus Kit (Qiagen, Redwood City, CA) and SARS-CoV-CDC RUO primers and probes (Integrated DNA Technologies (IDT) ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acids were extracted at CNM using either QIAamp MinElute Virus Spin (DNA) or QIAamp Viral RNA Mini kits (Qiagen, Germantown, MD, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA from the control human TSCs as well as GATA2-KD and GATA3-KD human TSCs were isolated using RNeasy Mini Kit (Qiagen, Germantown, MD) per the manufacturer’s protocol with on-column deoxyribonuclease digestion ...
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bioRxiv - Cancer Biology 2024Quote: ... Targeted knockdown was conducted with 100nM ON-TARGETplus smartpool human c-MYC (mixture of 4 individual siRNA) and/or human KLF6-SV1 siRNA (Dharmacon, Lafayette, CO) with HiPerfect (Qiagen, Valencia, CA).
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bioRxiv - Neuroscience 2022Quote: Total RNA from 18 ONS cell samples (6 AD patients, 6 MCI and 6 HC) were extracted using Allprep universal kit (Qiagen cat. no. 80224). RNA-seq libraries were prepared using the Illumina TruSeq Stranded Total RNA library Prep Gold Kit (Illumina 20020598 ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 100 μg/mL of carbenicillin for 16 hr at 30°C followed by plasmid purification (Qiagen, 12991).
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bioRxiv - Genomics 2020Quote: ... Upstream regulators as well as predicted interactions among DEGs were derived from Ingenuity Pathway Analysis (IPA, v01–16, Qiagen). Cytoscape was used for visualizing networks (46) ...
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bioRxiv - Genomics 2020Quote: ... An aliquot (16 μl) of the enriched samples was amplified using the GeneRead DNA Library L amplification Kit (Qiagen) according to manufacturer’s instructions performing 14 cycles and purified using solid-phase paramagnetic bead technology ...
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bioRxiv - Developmental Biology 2019Quote: ... each collection lid was covered with 15μl of a 1:16 dilution of proteinase K in PKD buffer (Qiagen), incubated at room temperature for 5 min and subsequently snap frozen in dry ice and stored at −80°C overnight ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA extraction of gill tissue (N = 16 individuals per population) was performed using the DNeasy Blood & Tissue Kit (Qiagen). Further purification of the extracted DNA was done with NucleoSpin® gDNA Clean-up (Macherey-Nagel) ...
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bioRxiv - Microbiology 2019Quote: Total RNA was isolated from BMDM and ModeK cell culture 16 h post infection using the RNeasy kit (Qiagen) and reverse transcribed using the Maxima H Minus First Strand cDNA Synthesis kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: The DNA used for the 16S amplicon sequencing was extracted using QIAamp DNA Stool Mini Kit (QIAGEN, Venlo, Netherlands) with an added bead beating treatment as the first step ...
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bioRxiv - Microbiology 2023Quote: Sequences comparisons between 16S rRNA and bsh genes/BSH amino acid sequences were performed in CLC Genomics Workbench (Qiagen). 7α-HSDH sequence comparisons were performed using tblastn78 using the translated amino acid sequence from Clostridium absonum44.
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bioRxiv - Cell Biology 2021Quote: Oligonucleotides for siRNA included: human βPix (synthesized by Qiagen), human myosin-18A (synthesized by Qiagen)
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bioRxiv - Molecular Biology 2022Quote: ... Each reaction of the HRM assay included 6.25µl of Type-IT 2 x HRM buffer (QIAGEN, Germany), primers specific for the uidA E.coli housekeeping gene and molecular grade water was added to make a final volume of 12.5µl ...
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bioRxiv - Molecular Biology 2020Quote: Total RNAs were extracted from wild-type or F14R HAP1 with the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Total mRNA was isolated from OregonR or Dijon2000 wild-type stage 17 embryos (RNeasy Micro Kit, Qiagen), and total cDNA was prepared (Superscript III First-Strand Synthesis System ...
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bioRxiv - Plant Biology 2019Quote: ... total RNA was extracted from wild-type and mutant nodules using the RNeasy Mini kit (QIAGEN, Germany) and treated with DNase (Turbo DNase kit ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was extracted from wild-type mESCs and Pml KOs using RNeasy Mini Kit (QIAGEN, 74104) and treated with Turbo-DNase (Ambion ...
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bioRxiv - Genomics 2023Quote: The PCR was performed with the Multiplex PCR Kit (Type-It Microsatellite master mix; Qiagen, Hilden, Germany). The SSR primer mix consisted of eight primers (see Table 2 and Table 3) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the microsatellite loci were PCR[amplified in five separate multiplexed reactions using a Type It Kit (Qiagen). Fluorescently labelled PCR products were then resolved by electrophoresis on an ABI 3730xl capillary sequencer (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... and Vero E6 cells infected with 2 patient virus isolates was converted to cDNA using reverse transcriptase from qiaseq SARS-CoV-2 primer pool (Qiagen kit, cat no. 333896). APOBEC3b (sense ...
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bioRxiv - Developmental Biology 2021Quote: ... The samples were incubated at 37 °C for 16 hours overnight and were in turn cleaned-up using the QIAquick PCR Purification kit (QIAGEN) and eluted with 40 μl of 50 °C water ...
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bioRxiv - Genetics 2020Quote: ... Ligation was carried out overnight at 16°C followed by overnight cross-link removal with 20mg/ml Proteinase K (Qiagen). The samples were purified using phenol-chloroform and ethanol precipitated resulting in 3C libraries ...
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bioRxiv - Genomics 2020Quote: ... 25ng of the purified product was subjected to self-ligation at 16 °C overnight in a total volume of 50uls and column purified using Qiagen (Qiagen) PCR purification kit as per manufacturers recommendations ...
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bioRxiv - Microbiology 2019Quote: ... The complete 16S rRNA gene was amplified in specific strains after DNA extraction using the DNeasy Blood & Tissue kit (Qiagen), following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2019Quote: ... After 16 hrs in a 37°C shaker bacteria were harvested and plasmid DNA was isolated using a Megaprep kit (Qiagen). ITR integrity was confirmed by digestion with XmaI as well as with AhdI ...
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bioRxiv - Genetics 2019Quote: ... Cross-links were reversed at 65°C overnight (16 hrs) and DNA was purified using a PCR purification kit (Qiagen). DNA content was quantified by RT-PCR using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... 16 equivalent slices were obtained from two roots (replicates) and collected in individual tubes containing RTL buffer (Qiagen MicroRNAeasy Kit). Samples were flash frozen by placing liquid nitrogen in the tubes ...
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bioRxiv - Cancer Biology 2021Quote: Driver mutations were derived from.15 CopyNumbers were calculated using the CNVKit package.16 RNA was isolated from cell cultures (+/− 5 μM AGI-5198) using the RNeasy kit (Qiagen). We performed paired-end sequencing of 2×100 with the Illumina Novaseq platform to obtain 8-10 GB per sample ...
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bioRxiv - Genomics 2021Quote: ... a 1.0 μL aliquot of each sample was amplified in 10 μL reactions incubated at 30°C for 16 h as described in the REPLI-g Mini Kit (QIAGEN).
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bioRxiv - Cell Biology 2020Quote: Full grown 7 days-old organoids from passages 11 to 16 were washed from Matrigel with ice-cold PBS and DNA was extracted using DNeasy Blood & Tissue Kit (Qiagen), following manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The quality of each sequenced pool was assessed using FastQC.16 The bisulfite sequencing plugin for the CLC Genomics Workbench (Qiagen) was utilized to analyze the reads which were mapped to the hg19 reference genome with a minimum acceptable alignment of >80% ...
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bioRxiv - Genomics 2020Quote: ... Amplified DNA (2ul from a 1:20 dilution of a 16 h REPLI-g Mini Kit whole-genome amplification, Qiagen) from each sorted population was tested in a 96-well plate in duplicate according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were induced with 100 μM IPTG at 12°C for 16 hrs and purified on a Ni2+ column according to manufacturer protocols (Qiagen). Proteins were eluted using 125 mM imidazole ...
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bioRxiv - Plant Biology 2022Quote: ... The tissue was then ground to a fine powder using 3/16 inch (4.76mm) ball bearings in a Tissuelyzer II (Qiagen, Germany) in the presence of dry ice in the pockets around tube holders ...
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bioRxiv - Genomics 2022Quote: ... The mixture was incubated at 16°C for 2 h and the resulting double-stranded DNA was purified using the MinElute PCR Purification kit (Qiagen) with 20μl EB for elution ...