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Citations for Qiagen :
401 - 450 of 10000+ citations for Mouse 14 3 3 protein sigma SFN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was briefly incubated (approximately 5 min) with 3 ml Ni-NTA Agarose (QIAGEN) before flow-through was collected ...
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bioRxiv - Cell Biology 2021Quote: AllPrep DNA/RNA/Protein Mini Kits (Qiagen) were used to extract total proteins from UVA-exposed and non-exposed HTEpC from all experiments ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA from the drug-resistant cells was isolated 14 days post transfection using DNeasy Blood & Tissue Kit (Qiagen) and analyzed for the presence of the LeishIF4E2 gene ...
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bioRxiv - Plant Biology 2020Quote: ... gibba fresh tissue 14-days old was grinded with liquid nitrogen and purified with DNeasy Plant Maxi Kit (Qiagen). For the library preparation ...
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bioRxiv - Cancer Biology 2021Quote: ... Those PDTOs that underwent FOLFOX treatment (n=14) had RNA extracted using the Qiagen RNAeasy micro kit (Qiagen, # 74004).
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from LMNs on days 14 and 45 using an RNeasy Mini kit (Qiagen, Hilden, Germany) with DNase I treatment ...
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bioRxiv - Cancer Biology 2022Quote: Whole tissue protein was extracted by Qproteome Mammalian Protein Prep Kit (Qiagen, 37901) according to the manufacture’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein were extracted using the AllPrep DNA/RNA/Protein kit (Qiagen, #47054). Sample concentrations were measured with Qubit high sensitivity dsDNA and RNA platform ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μL of 10 μM double-stranded mature miRNA mimic (Dharmacon) or 20 μM LNA (Qiagen) was mixed with 3 μL lipofectamine RNAiMAX in 100 μL Opti-MEM reduced serum media and added to cells following a 15-minute incubation ...
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bioRxiv - Microbiology 2022Quote: ... The soluble extracts were mixed for 30 min with 3 ml of Ni2+-NTA-agarose (Qiagen) that had been equilibrated with buffer A containing 10 mM imidazole ...
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bioRxiv - Genetics 2022Quote: ... Midi, 3-4 ml of blood) and Puregene (0.3-1 ml of blood, manual extraction) (Qiagen, Cat# 1057048 ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 million cells were seeded and transfected with 1μg pMK33-based plasmid DNA using Effectene (Qiagen). After 48 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... 3 μL water and 5 μL 2x QuantiFast® SYBR® Green PCR Master Mix (Qiagen). Technical duplicates of this reaction mix were then analyzed on a Corbett Rotor-Gene 6000 real-time PCR cycler ...
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bioRxiv - Cell Biology 2022Quote: ... A Locked Nucleic Acid (LNA) probe was used to detect centromere regions (5’-TTGGCTACACCATGAAAGCTT-3’; Qiagen). 20 μL of probe mix (250 nM LNA probe ...
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bioRxiv - Microbiology 2022Quote: ... 3 mL samples were added to tubes containing 6 mL RNAprotect Bacteria Reagent (Qiagen, Hilden, Germany) and vortexed ...
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bioRxiv - Microbiology 2023Quote: ... whole eyes were homogenized in PBS using a TissueLyser II (Qiagen, 30 Hz for 3 minutes), and homogenates were serially diluted and streaked on LB plates for quantification of colony forming units (CFU ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatant was added to 3 ml of Ni-nitrilotriacetic acid (NTA) superflow resin (Qiagen) and the mixture was applied onto a gravity drip column ...
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bioRxiv - Microbiology 2023Quote: ... Samples were mechanically disrupted for 2x 3 min at 30 Hz (TissueLyser II, Qiagen, Hilden, Germany). This was followed by adding 5 μl of proteinase K (20 mg/ml ...
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bioRxiv - Genomics 2022Quote: ... 12 µl proteinase K (14-22 mg/mL, Qiagen), and 12 µl β– mercaptoethanol) ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was extracted at days 0 and day 14 after infection using the QIAmp Blood and Cell Culture DNA midi kit (Qiagen). The PCR was performed by QMUL Genome Centre.
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bioRxiv - Immunology 2019Quote: ... T cells (from pooled spleen and pLNs in Figure 4 or from mLN in Supplementary Figure 6) from Foxp3Cre WT mice and Tet2/3fl/flFoxp3Cre DKO mice (14-weeks old) using RNeasy plus mini kit (Qiagen). RNA-sequencing libraries were prepared using Truseq stranded mRNA kit (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: All RNA was extracted from pellets of cultured cells (passage numbers ranging from 8-14) and cryosections of snap frozen tumor material with the RNeasy Plus Mini Kit (Qiagen).
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bioRxiv - Systems Biology 2020Quote: ... We incubated these cultures on a shaker at 225 rpm and 32°C for 12-14 hours then pelleted the cultures by centrifugation and isolated plasmid using the EndoFree plasmid maxiprep kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: RNA was extracted from 14-day-old wild-type and acinus-2 pinin-1 seedlings using RNeasy mini kit (Qiagen) and treated with TURBO DNA-free Kit (Ambion ...
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bioRxiv - Molecular Biology 2022Quote: ... and grown in serum-free medium for 24 hr and released into growth by addition of serum for 14 hr) by using RNA purification kit (QIAGEN). Purified RNAs were sequenced by GENEWIZ ...
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bioRxiv - Physiology 2020Quote: Total cellular RNA was isolated from transverse cryosections (14 μm) of murine ventricular myocardium using RNeasy Micro kits (Qiagen Inc.) with minor modifications ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was extracted from cells on fibers on days 14 and 28 of CM differentiation using a RNeasy Mini Kit (Qiagen), according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: RNA was extracted from separated shoots and roots of 14-d-old seedlings using RNeasy Plant Mini Kit (74904, QIAGEN). Quality check was carried out on a 2100 Bioanalyzer Instrument (G2939BA ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from dried and powdered 100 mg leaf or root tissue sample taken from pooled 14-d-old seedlings using RNeasy Plant Mini Kit (74904, QIAGEN). RNA was then extracted per the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from roots of a 14-day-old Col and GFP-GrxS5 plants grown on 10 mM NO3- using RNeasy Mini Kit (QIAGEN). We used 1 μg total RNA for mRNA purification using the NEBNext Oligo d (T ...
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bioRxiv - Molecular Biology 2024Quote: miRNAs of 14 gonads (eight testes and six ovaries) were obtained by miRNAs isolation commercial kit (Qiagen® miRNA, 217004). Quality of the samples was assessed by BioAnalyzer (2100 Bioanalyzer ...
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bioRxiv - Immunology 2024Quote: ... 1 μl of second round PCR product as the template in a new 14 μl reaction using HotStartTaq Master Mix Kit (QIAGEN). Each well was barcoded ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein was extracted using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen #80004) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein was purified by the AllPrep DNA/RNA/Protein Mini Kit (Qiagen: # 80004). Corresponding protein expression levels in the cells of different groups were detected by western blot using the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested for total protein using a Qproteome Mammalian Protein Prep Kit (Qiagen). Total protein was quantified using a PierceTM BCA Protein Assay Kit (Cat ...
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bioRxiv - Cancer Biology 2020Quote: The All Prep DNA/RNA/Protein kit (QIAGEN) was used to total RNA from cell lines ...
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bioRxiv - Genomics 2020Quote: ... The primer used in the 3’ RACE assay was designed using CLC Genomic Workbench (ver. 10.1.1, Qiagen) near the highest CAGE-Seq signal in the determined TSS region ...
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bioRxiv - Molecular Biology 2019Quote: Body tissue of medaka at 3 dph was minced and then processed with a RNeasy Minikit (Qiagen) for extraction of total RNA ...
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bioRxiv - Neuroscience 2021Quote: ... matching a sequence in intron 22 of the HTT gene (5’-TAATACGTAAGTGTCACAA-3’, custom synthesized by Qiagen) was delivered by intracerebroventricular (ICV ...
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bioRxiv - Microbiology 2021Quote: ... Pelleted cells were resuspended in 1 ml of Na2CO3 buffer containing 3 µg/ml RNase A (Qiagen) and incubated at 50°C for at least 4 h ...
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bioRxiv - Microbiology 2022Quote: ... 1ml of PBS-AB was added to each sample along with Tungsten carbide 3 mm beads (Qiagen). Samples were homogenized for 10 min and then centrifuged at 15,000 g for 10 min ...
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bioRxiv - Immunology 2021Quote: ... The samples (N=3) were collected immediately and soaked in 10 volumes of RNAlater (Qiagen, Hilden, Germany), for sequencing using Illumina (New England Biolabs) ...
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bioRxiv - Immunology 2020Quote: ... Cells were then washed 3 x with ice-cold 1x PBS and lysed in RLT buffer (Qiagen) with 1/100 β-mercaptoethanol (Sigma ...
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Surveying the landscape of tRNA modifications by combining tRNA sequencing and RNA mass spectrometrybioRxiv - Biochemistry 2019Quote: 400-1000 ng isolated tRNAs were digested in 3 μl aliquot with 20 ng RNase A (QIAGEN) in 10 mM NH4OAc pH 7 or 20 unit RNase T1 in 10 mM NH4OAc pH 5.3 at 37 °C for 1 hr ...
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bioRxiv - Microbiology 2020Quote: ... and 6.25mL 2-mercaptoethanol (βME)) and homogenized at 30Hz for 3 min in a TissueLyzer II (Qiagen). 60 μL of 100% isopropanol was added to each tube and incubated for 1 min ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA (>200nt) was extracted from 400 testes of ≤3 days old flies using miRNeasy Mini column (QIAGEN). For the first replicate ...
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bioRxiv - Cancer Biology 2023Quote: Small interfering RNA was obtained to knockdown the expression of ARSB and galectin-3 (Qiagen, Germantown, MD). Effects on mRNA were determined by QRT-PCR ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant from the second spin was incubated with 3 mL of Ni-NTA agarose beads (Qiagen) for 30 minutes at 4°C in the cold room ...
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bioRxiv - Cell Biology 2020Quote: ... for mouse cells or the RNeasy kit (Qiagen) for human cells ...
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bioRxiv - Microbiology 2020Quote: ... two replicates of two filter poresizes at four depths from 14 stations) following manufacturer’s protocols for the DNeasy PowerWater Kit (Qiagen, Hilden, Germany) including the optional heating step for 10 min at 65°C to fully lyse cells ...