Labshake search
Citations for Qiagen :
401 - 450 of 10000+ citations for Creatinine Serum Low Sample Volume Kit 384 well Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: RNA samples were prepared using the RNAeasy kit (Qiagen, 74104) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... RNA samples were prepared using RNeasy kits (QIAGEN, Hilden, Germany). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from samples (miRNeasy mini kit; Qiagen), quantified using Nanodrop 8000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were loaded onto RNeasy Maxi Kit (Qiagen Cat# 75152) columns and the RNeasy protocol for RNA washes and elution was followed (RNeasy Midi/Maxi Handbook ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples were prepared by using RNeasy Mini kits (Qiagen), followed by DNA depletion with Turbo DNase (Ambion ...
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bioRxiv - Plant Biology 2020Quote: Quantitative real-time PCR was performed in a volume of 5 mL QuantiTect Probe PCR Kit (Qiagen GmbH, Hilden, Germany) kit and an ABI 7900HT fast real-time PCR system (ThermoFisher Scientific Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... The colorless phase was then collected and combined with equal volume of 70% ethanol and used as input in the RNeasy Micro kit (Qiagen), where we followed the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The 3 replicate PCR reactions were pooled for a total volume of 60 μL and cleaned using a MinElute PCR Purification kit (cat. no. 28004, Qiagen) according to vendor instructions ...
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bioRxiv - Genomics 2020Quote: ... The aqueous phase was removed and mixed with ½ volume of 100% ethanol before adding to a column of the RNeasy kit (Qiagen). After addition to the column ...
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bioRxiv - Immunology 2021Quote: ... The total volume was then transferred to a RNeasy Mini Spin Column of the RNeasy Mini Kit (Qiagen, Hilden, Germany). Subsequent steps were conducted as described in the manufactureŕs protocol ...
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bioRxiv - Molecular Biology 2019Quote: Reverse transcription (RT) of total RNA to cDNA was performed in 50 μL reaction volumes using the miRCURY RT kit (Qiagen) and 10 μL total RNA as input ...
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bioRxiv - Microbiology 2019Quote: ... 500 ng of RNA were reverse transcribed in a final volume of 20 µl using QuantiTect Reverse Transcription Kit (Qiagen). Real-time PCR was performed using SYBR Green PowerUp (ThermoScientific ...
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bioRxiv - Systems Biology 2020Quote: ... For extraction of total RNA from freshly obtained and chilled anticoagulated blood was mixed with an equal volume of Buffer EL of the QIAamp RNA Blood Mini Kit (Qiagen) and then processed with the kit and Qiagen QIAshredder spin columns according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... the aqueous phase was added to an equal volume of 70% ethanol and used as the starting material for the RNeasy Mini Kit (74104, QIAGEN). RNA concentration and quality were assessed using a NanoDrop™ OneC Microvolume UV-Vis Spectrophotometer (840274200 ...
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bioRxiv - Microbiology 2022Quote: ... 500 µl of the upper phase were mixed with an equal volume of 70% EtOH and loaded on a RNeasy mini-kit column (Qiagen). After an on-column DNase treatment (RNase-free DNase set ...
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bioRxiv - Neuroscience 2022Quote: ... For the other samples total RNA was mixed with an equal volume of 70% ethanol and further purified with miRNeasy Mini Kit (217004, Qiagen) using the manufacturer’s protocol (Supplementary Table S1) ...
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bioRxiv - Immunology 2020Quote: ... Equal volumes of barcoded PCR2 products (5 μL each) were pooled and PCR column purified using QIAquick PCR Purification Kit (Qiagen). Libraries were quantified using KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplifications were carried out in 50-μL total volumes containing 1 μL of template using a DNA HotStarTaq Master Mix kit (Qiagen). The cycling protocol was as follows ...
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bioRxiv - Genomics 2020Quote: ... The lysate supernatant was mixed with an equivalent volume of freshly prepared 70% ethanol and purified using the RNeasy RNA isolation Kit (Qiagen). mRNA was extracted from approximately 5 μg of the total RNA samples using Sera-Mag oligo-dT beads (GE Healthcare Life Sciences) ...
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bioRxiv - Genomics 2019Quote: ... and then pooled for a single PCR cleanup with the QIAquick 96 PCR purification kit (Qiagen; 60 μL elution volume). Agarose (1.5% w/v ...
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bioRxiv - Immunology 2021Quote: ... Complementary deoxyribonucleic acid (cDNA) was synthesised from every 1 μg of total mRNA in 20 μL volume with QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacture protocol ...
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bioRxiv - Immunology 2022Quote: ... One volume of 70% ethanol was added and then transferred to a column from the RNeasy mini kit (Qiagen, 74104). RNA isolation was performed according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The aqueous phase was added to an equal volume of 70% ethanol and transferred to a column from the RNeasy Mini Kit (74104, QIAGEN). The protocol for RNA extraction was carried out according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 3 μL were used as template in a 10 μL final volume reaction using the miRCURY LNA SYBR Green PCR Kit (Qiagen) in a StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Systems Biology 2024Quote: ... To this 200uL of resuspended pellet we added 1 volume (200uL) of the QX1 buffer from the Qiaex2 DNA extraction kit (Qiagen) [56] ...
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bioRxiv - Immunology 2024Quote: ... The upper aqueous layer was mixed with an equal volume of 100% ethanol and processed using miRNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Genetics 2024Quote: ... qRT-PCR was performed in a 10 μL volume using the miRCURY LNA SYBR Green PCR Kit (Qiagen, Hilden, Germany) and the following miRCURY LNA miRNA PCR primer sets (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... The aqueous phase was then mixed with an equal volume of 70% ethanol and transferred to a silica column from the RNeasy Mini kit (Qiagen). Total RNAs were then purified according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... These samples were extracted with the QIAamp DNA Mini Kit and Gentra Puregene kit (QIAGEN). One additional lab (Lab 1 ...
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bioRxiv - Molecular Biology 2019Quote: Cells seeded in 24-well plates were transfected with siRNA against intended targets (Qiagen, sequences provided in Table S3) using Lipofectamine RNAiMAX (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were plated in 12 well plates at a dilution of 1.5×106 and were transfected with the indicated constructs using Effectene (Qiagen) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: 2.0 × 105 HeLa cells were transfected in 12 well plates with control (NTC: all-stars non-targeting control, Qiagen) or DDX39B-targeting (D11 ...
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bioRxiv - Plant Biology 2022Quote: ... Sections were immediately placed into 96-well plates (on ice) containing 10 μL of RLT plus (Qiagen, Hilden, Germany). All instruments and surfaces were cleaned with 80% ethanol ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from hESCs or NPCs grown in a 6-well plate (9.5 cm2) using the RNeasy minikit (Qiagen). A DNase 1 digestion step was performed during RNA extraction to degrade remaining genomic DNA ...
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bioRxiv - Immunology 2020Quote: ... ConM-SOSIP.v7 carrying a C-terminal His-tag (diluted to 6.5nM in TBS) was immobilized onto 96-well Ni-NTA ELISA plates (Qiagen) by incubation for 2 hours at room temperature after which the plates were washed 3 times with TBS ...
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bioRxiv - Immunology 2022Quote: ... Single cells from three to four mice per each condition were index-sorted directly into 96-well plates containing 5μl of TCL buffer (Qiagen) supplemented with 1%β-mercaptoethanol using a BD FACS Aria II instrument ...
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bioRxiv - Molecular Biology 2019Quote: 1×106 MIA PaCa-2 or HAP1 cells were seeded per 6 well plate and co-transfected with 0.5 µg pCas-Guide plasmid using Effectene transfection reagent (Qiagen). 24 hrs after transfection ...
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bioRxiv - Immunology 2021Quote: ... viable in vitro differentiated CD4+ T cells were sorted into 96-well plates containing 5 μl TCL Buffer (QIAGEN) with 1% 2-mercaptoethanol ...
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bioRxiv - Immunology 2022Quote: His-tagged RBD of SARS-CoV S was diluted to a concentration of 0.8 μg/mL in Tris-buffered saline (TBS) and immobilized on NiNTA 96-well plates (Qiagen) for 2h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded in 24-well plates and transfected with the desired plasmids using Effectene Transfection Reagent (#301427, QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the total RNA was extracted from the low-passage infected cell culture supernatant (500 µl) using an RNeasy Mini Kit (QIAGEN, Germany), and cDNA synthesis was carried out using an oligo d(T)15 primer and MMLV reverse transcriptase (Promega ...
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bioRxiv - Microbiology 2021Quote: ... The amplified products were analyzed onto 1% agarose gel (low EEO) and extracted and purified from the gel using the QIAprep Miniprep Kit (Qiagen, Netherlands). The 16S rRNA sequencing reactions were performed using 785F (5’-GGATTAGATACCCTGGTA-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... RNA-bead complexes were washed in high and low salt buffer and RNA was eluted from the RNA-bead complexes using the Qiagen RNeasy Plus Micro Kit (Qiagen 74034). Isolated m6A-enriched RNA was immunoprecipitated a second time using 2 µg of a second anti-m6A antibody (Abcam ab151230) ...
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bioRxiv - Microbiology 2022Quote: The total DNA was extracted from 0.25g of each sample/sample pellet using the DNeasy PowerSoil Kit (Qiagen). The quality and quantity of extracted DNA were examined using a DeNovix DS-11 spectrophotometer and an Invitrogen Qubit Fluorometer (dsDNA high-sensitivity assay kit ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from the gut samples (7-10 guts/sample) using the RNeasy Mini kit (Qiagen) and the on-column DNase I treatment (79254 ...
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bioRxiv - Microbiology 2020Quote: Nucleic acids were extracted from mouse fecal samples and inoculum samples using the DNeasy Powersoil HTP Kit (QIAGEN) and from the further simplified SC2 samples using the Powermag Microbiome kit (MoBio) ...
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bioRxiv - Developmental Biology 2020Quote: ... All samples were pooled in equimolar ratio and PCR purified pooled sample with QIAquick PCR Purification Kit (Qiagen). The purified sample was then run on a 2% E-Gel and the correct size band was extracted for PCR2 (250-270 bp ...
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bioRxiv - Genetics 2023Quote: DNA was extracted from blood samples of 4,723 individuals (4,594 population sample ESSE + 129 Starvation Controls) using the QIAamp DNA Mini Kit (Qiagen) and genotyped using a custom FinnGen ThermoFisher Axiom microarray [2] ...
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bioRxiv - Bioengineering 2023Quote: ... total RNA was isolated from Hep3B cell spheroids on the pillar plate and in the ULA plate using a RNeasy Plus kit (Qiagen,74134). One μg of RNA was converted into cDNA using the high-capacity cDNA reverse transcription kit according to the manufacturer instructions (Applied Biosystems ...
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bioRxiv - Bioengineering 2019Quote: ... The exosomal RNAs were extracted using the exoRNeasy Serum/Plasma Starter Kit (Qiagen, 77023) following the vendor’s instruction ...