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Citations for Qiagen :
401 - 433 of 433 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The spheres were washed twice and stained with 1:500 PI solution (Bio-Sciences) supplemented with 250 μg/mL RNAse A (Qiagen) and Hoechst nuclear counterstain (2 μg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... Total DNAs were extracted from the aqueous phase/ethanol solutions according to the manufacturer’s protocol using the DNAEasy Blood and Tissue Kit (Qiagen, Germantown, MD). Total DNA concentrations and purities were measured using NanoDrop (Thermo Fischer ...
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bioRxiv - Genomics 2020Quote: ... pupae were immediately transferred from −80° C into a 50mL conical tube containing pre-prepared lysis solution consisting of 9.5mL Buffer G2 and 19μL RNase A (Qiagen Cat No. 19101). The pupae were then homogenized using a Dremel motorized homogenizer for approximately 30 seconds on the lowest speed ...
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bioRxiv - Immunology 2020Quote: Initial crystallization hits were obtained via sparse matrix screening in 96-well plates using commercially available crystallographic solutions (Qiagen, Toronto, ON). Optimization of crystallization conditions was performed in 24-well plate format using the hanging drop vapor diffusion method ...
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bioRxiv - Molecular Biology 2022Quote: Nunc MaxiSorp™ 96-well plates were coated with 75 ng/well of penta-His antibody in PBS solution (Qiagen, 34660) and incubated overnight at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... 30µL of DNase and RDD buffer (1:7 ratio) containing solution from the RNase-Free DNase Set (Qiagen, Catalog No. 79254) was incubated on the columns at room temperature for 15 minutes before a second and third round of RNA was performed as well as a final spin to ensure the column was dry ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was extracted after disrupting the frozen leaves with metal beads in RLT and β mercaptoethanol solution using a Qiagen RNeasy plant mini kit (Qiagen) according to manufacturer’s instructions (without the optional DNase step) ...
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bioRxiv - Microbiology 2023Quote: ... cells were collected by centrifugation and quickly washed with ice-cold water to remove the methanol and resuspended in RNAlater solution (Qiagen, 76104) for at least 2 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue lysis was performed by re-suspending ∼30mg of sliced frozen tissue in a solution containing 1% β-mercaptoethanol in RLT buffer (supplemented with antifoam agent; ID 19088, Qiagen). Next ...
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bioRxiv - Microbiology 2023Quote: ... Stigma tissues were removed from tube and DNA was extracted from the solution using DNeasy PowerSoil Pro Kit (Qiagen, Hilden Germany) following manufacturer’s instructions except the following modifications ...
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bioRxiv - Microbiology 2024Quote: ... all samples were centrifuged at 5000rpm in a table-top centrifuge and the pellet was resuspended in CD1 solution of DNAeasy plant pro kit (Qiagen; 0142924730) along with 0.25-0.5 mm glass beads ...
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bioRxiv - Pathology 2024Quote: DNA was extracted from the pooled skin lesion samples stored in the RNA preserving solution using the QIAsymphony® DSP Virus/Pathogen Kit (Qiagen). A species-specific PCR assay based on a previously published protocol [37] ...
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bioRxiv - Genomics 2024Quote: ... a buffy coat from a fresh 5-ml blood sample tube was aliquoted for peripheral blood mononuclear cells (PBMCs) isolation using RBC Lysis Solution (QIAGEN, 158904). The purified PBMCs were counted ...
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bioRxiv - Developmental Biology 2021Quote: ... Every other hour ~300 embryos were counted and lysed in 350μl of a solution of RLT buffer and β-mercaptoethanol from the Qiagen RNeasy Micro Kit (Qiagen, Hilden, Germany). The lysates were immediately stored at −80°C until use ...
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bioRxiv - Cancer Biology 2022Quote: ... and areas containing >30% tumor cells as determined by an expert pathologist were macrodissected and deparaffinated using Deparaffinization Solution (Qiagen, Hilden, Germany). DNA was extracted and purified with GeneRead DNA FFPE Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... For the detection of phosphorylated Serine residues a TBS solution with a 1:100 dilution of the Anti-Phospho-Serin Antibody (Qiagen, Hilden, Germany) was used ...
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bioRxiv - Microbiology 2020Quote: ... an aliquot of the extracted RNA solution was added to the reaction mixture for the QuantiTect probe RT-PCR kit (Qiagen, Hilden, Germany), which contained 2x QuantiTect probe RT-PCR master mix ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from primary solution with the removal of genomic DNA by the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). The purity and concentration of extrasted totel RNA were confirmed using a NanoDrop ONE spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA removal was performed by applying 80 μl of DNase I working solution (10 μL DNase stock + 70 μL 1x RDD buffer; RNase-Free DNase Set, Qiagen, Hilden, Germany) to the column and incubated for 15 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: Nineteen colonies isolated were resuspended in sterile PBS solution and the DNA was extracted using the DNeasy Powerlyzer Microbial Kit protocol (Qiagen, Hilden, Germany). After DNA extraction ...
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bioRxiv - Microbiology 2022Quote: ... 2 g of soil was immediately transferred into pre-weighed sterile tubes containing 3 ml of LifeGuard soil preservation solution (Qiagen, Toronto, CA) to stabilize the RNA ...
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bioRxiv - Microbiology 2024Quote: ... from which total RNA was extracted by following an established protocol using the RNeasy mini-kit and an RNase-free DNase I solution (Qiagen, Germantown, MD) for in-column gDNA removal (24) ...
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bioRxiv - Microbiology 2024Quote: ... 200 µl aliquots of the sample solution were processed by either the in-house method or using the DNeasy Blood & Tissue Kit (Qiagen Cat 69504) per manufacturers’ instructions.
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bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA was extracted from tissue and blood in salt solution using a DNeasy Blood and Tissue kit (QIAGEN Pty Ltd, VIC, Australia) or a Gentra Puregene Blood Kit (QIAGEN Pty Ltd ...
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bioRxiv - Genetics 2022Quote: ... The tissue powder was then transferred to a 1.5 mL centrifuge tube where 400 μL of 1% PVP-40 Buffer AP1 solution and 4 μL of RNase A were added (Qiagen DNeasy Plant Kit Qiagen Inc, Hilden, Germany). The tube was initially vortexed to homogenize the solution before incubation at 65 °C for 30 minutes with a short vortex every 5 minutes.
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bioRxiv - Cancer Biology 2023Quote: ... DNA was eluted with 66 µL of 2 mM Tris-HCl pH=7.0 to carry out Solution A purification or eluted with 66 µL of 0.1 X EB buffer (From QIAGEN Plasmid Plus Midi Kit) without further Solution A purification.
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bioRxiv - Cancer Biology 2023Quote: ... Microdissection was performed manually on the tumour Gleason grades marked FFPE areas using a sterile injection needle and deparaffinized using deparaffinization solution (Qiagen, Catalog number – 19093). RNA extraction was performed using the miRNeasy FFPE kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... Whole ear tissues were homogenised in sterile 1.5 ml flat-bottom tubes containing 400 µl of PowerBead Solution (Part of DNeasy UltraClean Microbial Kit; Qiagen, Cat #12224-250, Hilden, Germany) with an OMNI Tissue Homogeniser with plastic hard-tissue probes (OMNI International) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in Propidium Iodide (PI) staining solution (1x PBS supplemented with 50 µg/mL PI, Invitrogen; 100 µg/mL RNase A, Qiagen; and 2 mM MgCl2) and incubated for 30 min at room temperature in the dark) ...
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bioRxiv - Genomics 2023Quote: ... DNA for library preparations and sequencing was extracted using a standard phenol-chloroform extraction from liver and muscle samples which were collected at slaughter and stored in stored in RNAlater® Solution (Ambion/QIAGEN, Valencia, CA, USA).
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bioRxiv - Microbiology 2024Quote: ... Biomass was immediately stabilized upon sampling by mixing 2 ml biomass with 6 ml PowerProtect DNA/RNA solution (1:3) (Qiagen Benelux B.V., Venlo, The Netherlands). The stabilized mixture was spun down and the remaining pellet was freeze-dried overnight and stored at −70°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... 25 µL of samples was mixed with Triton X-100 solution and then incubated with 25 µL of Proteinase K (ProK; Qiagen, 19131; 100 µg/mL in PBS) at 37°C for 30 min prior to Tluc measurement.
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bioRxiv - Immunology 2023Quote: ... The first round of PCR was carried out using the ImmunoSEQ proprietary PCR primer mix (32 μL per sample containing 25 uL of QIAGEN 2× Multiplex PCR Master Mix, 5μL of QIAGEN 5x Q-solution and 2 μL of primer mix). A positive control reaction ...