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Citations for Qiagen :
401 - 450 of 1690 citations for 4 3 6 Dimethyl 3 heptyl phenol monoethoxylate ring 13C6 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... LRRCC1-si2 (target sequence: 5’- TTA GAT GAC CAA ATT CTA CAA - 3’) and control siRNA (AllStars Negative Control) were purchased from Qiagen. siRNAs were delivered into cells using Lipofectamine RNAiMAX diluted in OptiMEM medium (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA was extracted from fresh leaves of 2 to 3-week-old seedlings for all lines using a Qiagen MiniPrep Kit (Qiagen). Genotyping of all lines was performed using a custom lentil exome capture assay as described in Ogutcen et al ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: 3-10k cells were FACS sorted into buffer RLT with β-mercaptoethanol and total RNA prepared using RNAeasy Micro (Qiagen) with on column DNase I treatment ...
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bioRxiv - Genomics 2020Quote: ... and template-switching oligo (5′-AAGCAGTGGTATCAACGCAGAGTACrGrG+G-3′, where “r” indicates a ribonucleic acid base and “+” indicates a locked nucleic acid base; Qiagen). cDNA was amplified using KAPA HiFi HotStart ReadyMix kit (Roche #KK2502 ...
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bioRxiv - Bioengineering 2020Quote: Around 250 mg of each produce sample was cut into 1-3 mm pieces using a scalpel and then processed with the DNeasy Powersoil Pro kit (Qiagen) to isolate 50μL of eluted DNA ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was isolated from the rosette leaves of healthy 3-week-old plants (before yellow necrotic lesions develop) using the RNeasy Plant Mini Kit (Qiagen). All results shown include data from three biological replicates ...
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bioRxiv - Immunology 2021Quote: ... Each AES was then amplified by PCR on a 3 kb fragment using primer X5 and X6 and purified using QIAquick PCR Purification kit (Qiagen). For gene yrbE4A ...
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bioRxiv - Immunology 2021Quote: Total RNA extracted from lungs on dpi 3 was reverse transcribed as described above and was mixed with RT2 SYBR Green ROX qPCR Mastermix (Qiagen) to perform RT² Profiler™ PCR Array Mouse Hypoxia Signaling Pathway (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... including miRNA was isolated from brains of E11 mouse fetuses (3 biological replicates) at 2 h after irradiation using the miRNeasy Mini Kit (Qiagen). RNA was subsequently processed for hybridization to GeneChip miRNA 4.0 microarrays (Affymetrix ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... The temperature was increased from 25 °C to 95 °C at 3 °C per minute and fluorescence was measured at 610 nm in a Rotor-Gene Q thermocycler (Qiagen). The experiment was performed in technical triplicates ...
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bioRxiv - Neuroscience 2021Quote: ... (n=3 biological replicates) and TACs (hGFAP::GFP-, CD133-, EGFR+, CD24-) (n=2 biological replicates) using the miRNeasy kit (Qiagen). miRNAs were pre-amplified and profiled using TaqMan® Array Rodent MicroRNA A Cards v2.0 A as specified by the manufacturer at the Genome Technology Center of New York University Langone Medical Center ...
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bioRxiv - Plant Biology 2020Quote: ... and LIC2 adaptor at 3’(5’-gA ggA gAA gAg CCg Tcg-3’) by overhang PCR amplification and purified by gel-extraction using QIAquick Gel Extraction Kit (Qiagen). Then ...
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bioRxiv - Systems Biology 2020Quote: ... For extraction of RNA from frozen liver the Qiagen TissueLyser instrument with 3 mm stainless steel beads and the RNeasy Mini Kit (Qiagen) was used ...
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bioRxiv - Microbiology 2021Quote: ... and RNA was isolated from 3 biological replicates of each strain when the cultures had reached OD600 = 0.1 using the RNeasy mini kit (Qiagen #74106). RNA-Seq was performed at the Genomics Core Facility at Princeton University ...
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bioRxiv - Neuroscience 2020Quote: ... Genomic DNA from ∼250,000 neurons per sample (n = 3 samples per group) was extracted and purified (DNeasy Blood and Tissue DNA extraction kit, Qiagen) prior to RRBS (Ovation RRBS Methyl-Seq System ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was purified 1-3 dpi from mock- or POWV-infected hBMECs or hPCs using RLT lysis buffer and RNeasy columns (Qiagen). Purified RNA was quantitated ...
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bioRxiv - Microbiology 2020Quote: ... and supplemented with 1X EDTA and 1X Halt Protease inhibitor cocktail (Thermo-Scientific, CAT No.:78440) and were lysed using bead beating (50 Hz for 3 five-minute cycles, TissueLyzer II (Qiagen) with 0.1 mm silica beads) ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was isolated from 3D samples (n=2-3) by combining a TRIzol-based cell lysis with a RNeasy Mini Kit (Qiagen). Samples were collected at aforementioned timepoints ...
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bioRxiv - Genomics 2022Quote: ... Formalin-Fixed Paraffin-Embedded (FFPE) tissue (3 samples) and fresh frozen tissue (1 sample, QiaAmp Blood mini kit, QIAcube, QIAgen). Two samples are reference samples (NA24385 and NA12892 ...
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bioRxiv - Cell Biology 2022Quote: ... each sample was from a separate NRVM isolation from a total of 3 isolations) and homogenized in RLT buffer (Qiagen) using green RINO homogenization tubes following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: 400–1000 ng partial fragments of tRNA-Tyr were digested in 3 μl aliquot with 20 ng RNase A (QIAGEN) in 10 mM NH40Ac pH 7 ...
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bioRxiv - Genomics 2022Quote: ... the supernatants were mixed with Sigma HIS-Select Nickel Affinity Gel (at a ratio of 3:1) equilibrated in lysis buffer before being added into a polypropylene column (Qiagen). After extensive washing with a cold lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: Spheroid and monolayer samples (technical replicates n=3) of MUC-1 and NCI-H295R cells were processed for RNA extraction using the RNeasy Mini kit (Qiagen), followed by DNA removal (TURBO DNA-free™ Kit ...
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bioRxiv - Immunology 2022Quote: ... genomic DNA was isolated from 3×106 cells by spin-column based DNA purification according to the manufacturer instruction (DNeasy, Qiagen). Knockout efficiency was analyzed on the genomic level through TIDE analysis (Tracking of Indels by DEcomposition) ...
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bioRxiv - Cell Biology 2022Quote: ... The cleared lysate was then incubated with 3 mL of 1:1 Ni-NTA agarose slurry (Qiagen, product number 30210) for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from single-cell clone or bulk GFP+ and GFP- cells (n = 3 with cells from three different mice in each group) using QIAzol lysis reagent (Qiagen). DNA was removed using the RNase-Free DNase Set (QIAGEN) ...
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bioRxiv - Microbiology 2020Quote: ... and were ligated by PCR using primers Lpg499-DR and Lpg499-UF to generate a 3-kb fragment that was purified by a gel extraction kit (Qiagen). The purified 3-kb fragment was introduced into the KS79 strain by natural transformation (76 ...
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bioRxiv - Microbiology 2020Quote: ... All samples were then passed 3-5 times through a 26G needle prior to RNA isolation using the RNAeasy mini kit from Qiagen. RNA concentrations were estimated by absorbance measurement at 260 and 280 nm ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was extracted from pooled (n=10) embryonic zebrafish and pooled (n=3) adult (> 1 year) heart samples by QIAGEN RNEasy Lipid Tissue Extraction kit according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... total RNA was extracted from control and INPP5ED477N/D477N organoids (n=3 samples per genotype) using an RNeasy Plus Micro Kit (Qiagen) and reverse transcribed using Superscript™ IV VILO™ Master ezDNase enzyme (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA was isolated from the rosette leaves (5th and 9th) of healthy 3-week-old plants using the RNeasy Plant Mini Kit (Qiagen) including DNase I treatment ...
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bioRxiv - Neuroscience 2021Quote: ... day 10 embryoid bodies (EBs) and D45-47 cortical organoids (3 pooled EBs or COs per sample) using RNeasy Mini kit (Qiagen). Approximately 0.5-1ug of RNA was used as input to generate cDNA using Superscript IV Reverse Transcriptase (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were washed with PBS to remove as much media as possible prior to resuspension in 3 mL RNA Protect Bacteria Reagent (Qiagen). After incubating for 5 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... Amplified DNA was run on a gel and a band the length of Nectin-3 (∼1800 bp) was cut from the gel and purified (Qiaquick Gel Extraction Kit, Qiagen). Sequences were then amplified (KOD hot start DNA polymerase ...
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bioRxiv - Molecular Biology 2022Quote: Qiagen miRCURY locked nucleic acid DIG (digoxigenin)-labeled probes (sense cATM-DIG: 5’DIG-AGTGGTTAGACAGTGATGTGT-DIG 3’) (Qiagen, Hilden, Germany) were used for ISH ...
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bioRxiv - Microbiology 2020Quote: ... templates containing a T7 phage promoter sequence (5’-TAATACGACTCACTATAGGG-3’) were amplified by PCR using corresponding primer pairs and were purified with a QIAquick PCR purification kit (Qiagen). The primers used are listed in S1 Table ...
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bioRxiv - Pathology 2021Quote: ... DNA was extracted from epidermal tissue (approximately 3 - 5 mm2 per lesion) using a DNAeasy blood and tissue kit (Qiagen). Regressed and CsA group mice were culled at day 133 post-infection ...
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bioRxiv - Genetics 2021Quote: ... and 99F8 (150 ng pU6-3-99F8-gRNA (DGRC Cat# 1549) and 150 ng pBS-99F8-5’HR-attP>>Act5C::GFP<
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bioRxiv - Immunology 2021Quote: ... single MR1-tetramer-binding cells from Peruvian participant 7-3 and blood bank donors 702A and 703A were sorted into 96-well plate coated with Vapor-Lock (Qiagen) containing Iscript cDNA synthesis mixture (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... The LNA gapmers for lnc-FLii-1 and lnc-LSAMP-3 were designed and purchased along with negative control LNA from Qiagen. For transfection ...
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bioRxiv - Cell Biology 2021Quote: Formalin-fixed paraffin-embedded tissues were used for in situ hybridization (ISH) employing locked nucleic acid (LNA) probes labelled with digoxigenin (DIG) at both the 5′- and 3’-ends (Qiagen). ISH was performed with probes specific for miR-132-3p (10 nM ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA from the original material used for next-generation sequencing of the serum and passage 3 cultured MRI103 virus was used as the template for amplification using the One-Step Ahead RT-PCR kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... genomic DNA was extracted from cells 3 d after electroporation using a QIAamp DNA Mini Kit or Micro Kit (QIAGEN) per the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... for a total of 6 min (2 x 3 min with 1 min on ice in between homogenisations) at 50 Hz using a TissueLyser LT (Qiagen). Thereafter ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T and Neuro2a cells were harvested 3 days post-transfection and genomic DNA was extracted using a DNeasy Blood and Tissue kit (Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... homogenized in 1 ml PBS by bead beating (3 mm steel ball, 25 Hz for 1 min in a TissueLyser (Qiagen)) ...
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bioRxiv - Immunology 2021Quote: ... total RNA was isolated from 0.4×106 FACS-sorted B220hi CD138- and B220low CD138+ B cells (derived from 3 biological replicates after eight days in culture) using the RNeasy Mini Kit (Qiagen). cDNA was generated from polyadenylated transcripts employing the SMART-Seq v4 ultra low input RNA kit (Takara Bio) ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was extracted from edited and unedited cells 3 days post nucleofection using the DNeasy Blood and Tissue Kit (Qiagen). PCRs were performed with primers spanning the gRNA binding site (Supplementary Table 1) ...
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bioRxiv - Neuroscience 2022Quote: Tissue cultures that were cultivated on one filter membrane (3 cultures) were transferred as one sample into RLT buffer (QIAGEN) and RNA was isolated according to the manufacturer’s instructions (RNeasy Plus Micro Kit ...
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bioRxiv - Biophysics 2022Quote: ... The liquid culture was then grown for 3 hours at 37°C before the plasmid extraction using a miniprep kit (QIAGEN). Prepared DNA was sequenced by the Microbial Genome Sequencing Center (https://www.seqcenter.com/ ...