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Citations for Qiagen :
401 - 450 of 1696 citations for 2 Chloro 3 6 chlorohexanoyl pyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from hESCs or NPCs grown in a 6-well plate (9.5 cm2) using the RNeasy minikit (Qiagen). A DNase 1 digestion step was performed during RNA extraction to degrade remaining genomic DNA ...
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bioRxiv - Immunology 2020Quote: ... or IFNγ (10ng/mL) for 6 hours prior to harvesting and RNA extraction using RNeasy Mini Kit (Qiagen 74106). Next ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were then seeded in 6-well dishes (6x105 cells/well) and reverse transfected using Polyfect (Qiagen, 301105) following the manufacturers protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The expressed recombinant protein was solubilized in a denaturing buffer (6 M HCl-Guanidine, 20 mM Tris-HCl[pH 7.5] and purified by Ni-NTA (30210, QIAGEN) under denaturing conditions ...
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bioRxiv - Cell Biology 2022Quote: Cells were grown in 6 well plates and total mRNA was isolated using the RNeasy Kit (Qiagen, 74-104) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... after adding 1.0×10^6 copies/µL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... solubilized in a denaturing buffer (6 M HCl-Guanidine, 20 mM Tris-HCl pH 7.5) and purified by Ni-NTA (QIAGEN) under denaturing conditions ...
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bioRxiv - Genomics 2023Quote: ... Plasmid transfections were performed in six-well tissue-culture treated dishes at 1.8 × 10^6 cells/ml using Effectene (301427; QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted from ∼70% confluent HeLa cells grown in 6-wells plate using the RNeasy Micro Kit (Qiagen) with a DNase step according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2024Quote: Total mRNA was isolated from 6 hpf to 5 dpf zebrafish homogenates using an RNeasy Mini Kit (Qiagen, 74106) and reverse-transcribed with iScript (Bio Rad ...
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bioRxiv - Immunology 2021Quote: ... then diluted to 2 nM using elution buffer (Qiagen) with 0.1% Tween20 (Sigma) ...
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bioRxiv - Biophysics 2020Quote: ... 2 mM PMSF) on a Ni-NTA resin (Qiagen). Bound proteins were washed (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... then diluted to 2 nM using elution buffer (Qiagen) containing 0.1% Tween20 (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... the 2 proteases of proteinase K (Qiagen, Hilden, Germany) and dispaseII (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... 2 μl of 100 mg/mL RNase A (Qiagen) was added and the tubes were incubated at 37°C for 15 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µl Qiagen CL buffer (10x; Qiagen, Hilden, Germany), 0.4 µl MgCl2 (25 mM ...
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bioRxiv - Bioengineering 2023Quote: ... 2 volumes of RNAprotect Bacteria Reagent (Qiagen, Hilden, Germany) are added to one volume of bacterial culture ...
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bioRxiv - Neuroscience 2024Quote: ... [2] or QIAzol® Lysis reagent (Qiagen, #cat 79306) following vendor’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... packed with 2 mL Ni-NTA agarose resin (Qiagen) pre-equilibrated with 15 mL lysis buffer ...
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bioRxiv - Systems Biology 2024Quote: ... and 16.7% (v:v) Proteinase K (Qiagen, 19131, 2 mL). The suspension was then incubated at 37 ℃ for 15 minutes at 500 r.p.m.
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bioRxiv - Biophysics 2024Quote: ... A 2 mL Ni-NTA resin (Qiagen, Cat. # 30210) was equilibrated with 10 mL of 1X Base buffer and 5 mM β-mercaptoethanol for 10 min by constantly rotating ...
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bioRxiv - Cancer Biology 2024Quote: ... and sample incubated with 2 NiNTA resin (Qiagen, USA). Protein samples were allowed to bind for 4 hr ...
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bioRxiv - Microbiology 2020Quote: Whole genome amplifications were performed on DNA extracts at dilution 10 times through a multiple displacement amplification (MDA) step of 6 to 7 hours using the REPLI-g Midi Kit (QIAGEN) and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... we extracted total RNA from CD4+/CD8+/CD3+ T cells (from spleen or bone marrow) of Foxp3-GFP or C57BL/6 mice using RNeasy Micro Kit (Qiagen) and cleaned from excess DNA with DNAse 1 enzyme (Promega) ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from blood (n=6 for each housing group) using the RNeasy Protect Animal Blood Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... or trl RNA was was calculated using the 2-ΔΔCt method56 using RNA extracted from 500ul of cells following the 6 day incubation using an RNeasy Plus RNA extraction kit (Qiagen). Gene targets were amplified from cDNA using previously validated primers for clamp ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from frozen mouse liver (20-25 mg) and HepG2 cells (5-6 million) using the AllPrep DNA/RNA Mini Kit (QIAGEN) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Biochemistry 2020Quote: ... 5.6 x 105 cells were seeded in 4 ml DMEM in a 6-cm culture plate and transfected with targeting or control siRNA (Table 4) (from Qiagen) to a final concentration of 20 nM for each siRNA used ...
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bioRxiv - Neuroscience 2021Quote: ... SH-SY5Y cells were treated either with 100 μM cycloheximide (CHX) or DMSO for 6 hours (41) before harvesting the RNA through RNeasy Minikit (Qiagen). Reverse transcription was performed using RevertAid cDNA synthesis kit (Thermo) ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted by quantitative RT-PCR from Col-0 and DWF4-OE seedlings 6 days post germination with the RNeasy Mini Kit (Qiagen). For DWF-OE ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from cells (6-well dishes) or liver pieces (∼10-15mg) using the RNeasy Plus Mini kit (Qiagen). For livers ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of blood from K2-EDTA collection tubes was collected prior to centrifugation and was added to 600 μl of AVL viral lysis buffer with 6 μL carrier RNA (Qiagen) for RNA extraction ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from the leaves of 4-to-6-week old plants using the RNeasy Plant Mini Kit (Qiagen). RNA concentrations were quantified using a Qubit RNA BR Assay Kit (ThermoFisher ...
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bioRxiv - Plant Biology 2021Quote: ... We extracted total RNA from 10 leaves that were shorter than 500 µm and from 4–6 mature leaves using the RNeasy Micro Kit (Qiagen) and the RNeasy Plant Mini Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA was isolated from liver and lung tissues (n=4 for MEL077 and n=6-7 for MP46 per treatment group) using the QIAamp DNA Mini Kit (Qiagen) and for blood (n=3-4 for MEL077 and n=6-7 for MP46 per treatment group ...
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bioRxiv - Cancer Biology 2021Quote: ... and for blood (n=3-4 for MEL077 and n=6-7 for MP46 per treatment group) using the QIAamp DNA Blood Mini kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: DNA extraction from cultured tprAko-SS14 or wild-type strains propagated in 6-well plates following the transformation procedure was performed using the QIAamp mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... we transfected S2 cells in each well of a 6-well plate with 1 µg each of HA/GFP-tagged constructs using Effectene (Qiagen). After incubation for 3 days ...
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bioRxiv - Cancer Biology 2021Quote: ... 250 to 1000 μm lengths of epithelium sections were microdissected from 4 to 6 successive serial sections and DNA extracted using a QIAMP DNA microkit (Qiagen) by digesting overnight and following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cells were either left untreated or treated with 1000 IU/ml recombinant IFN-α treatment and incubated for 6 h or transfected overnight with polyI:C (10 μg/ml) using Polyfect (Qiagen). Luciferase assays were carried out ...
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bioRxiv - Microbiology 2021Quote: ... coli giant spheroplasts we used a N-terminally 6-His tagged version of TcMscS cloned into the expression plasmid pQE80 (Qiagen) with restriction sites BamHI and HindIII ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 and A549ACE2 cells in 6 well formats were homogenized using QIAShredder tubes and total RNA isolated using the RNEasy kit with DNase treatment (Qiagen). High quality RNA samples that were verified by a Bioanalyzer (Agilent ...
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bioRxiv - Genomics 2020Quote: ... and 3-week-old) and leaves (4-, 5-, and 6-week-old plants) was isolated and purified using RNeasy Plant Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified using Ni-NTA immobilized metal-affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow Resin (GE Lifesciences) or NiNTA Agarose (QIAGEN) and gravity flow ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from 10000 sorted cells (gated using strategies shown) from livers of C57BL/6 mice using a RNeasy Plus micro kit (QIAGEN). Sensifast cDNA synthesis kit (Bioline ...
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Single-cell assessment of trophoblast stem cell-based organoids as human placenta-modeling platformsbioRxiv - Developmental Biology 2022Quote: ... The methylation status of each CpG of interest (n=6 per sample) was evaluated with PyroMark Q-CpG software (Qiagen) and data from all CpG sites was averaged for each sample and presented as percent methylation (Figure S1E).
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was extracted from both hippocampi of 6 P12 C57BL/6J male mice using the miRNeasy mini kit (Qiagen). The sequencing pipeline is based on the Illumina’s Small RNA Digital Gene Expression v1.5 protocol with minor modifications to include indexing and multiplexing of RNA samples ...
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bioRxiv - Neuroscience 2022Quote: ... iPS-NES cells were plated in 6-well plates and lysates were collected in RNAprotect® Cell Reagent (QIAGEN, #76526) to stabilize the RNA ...
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bioRxiv - Immunology 2022Quote: ... diluted in fresh culture media or left untreated (n = 4) for 6 h prior to harvesting RNA in RLT buffer (Qiagen) with added β-ME ...