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Citations for Qiagen :
4201 - 4250 of 10000+ citations for Androstenedione ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Approximately 25 mg of frozen tissues were transferred to 2 mL Eppendorf Protein Lobind tubes containing one 5 mm stainless steel bead (cat# 69989, Qiagen) and 500 µL of lysis buffer consisting of 5% SDS in 50 mM TEAB with protease inhibitors cocktail (cat# A32953 ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was pelleted by centrifugation at 30,000 x g for 30 min and the supernatant was mixed with 5 mL pre-equilibrated Ni-NTA resin (Qiagen) in a 50 mL falcon tube ...
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bioRxiv - Genomics 2022Quote: ... We incubated the reaction for 5 minutes at 55 °C in a G-Storm GS1 (G-Storm) thermal cycler and after incubation added 5 μl Buffer PB (19066, QIAGEN) to inactivate and strip the transposase from the tagmented DNA ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and 1x antibiotic-antimycotic by a TissueLyser II bead mill with 5-mm stainless steel beads (Qiagen). After brief centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... calf muscle and pancreas were collected in 500 μl of PBS containing one (heart and pancreas) or two (calf muscle) 5 mm stainless steel beads (QIAGEN), homogenized with TissueLyser II (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... The sample was stored with RNAprotect following the manufacturer protocol (Protocol 5 from RNAprotect Bacteria Reagent Handbook 01/2020, Qiagen) at -80°C until processed ...
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bioRxiv - Biophysics 2023Quote: ... Cleaved PCNA was separated from His-SUMO and uncleaved His-SUMO-PCNA by incubating the protein solution with 5 ml Ni-NTA suspension (Qiagen) resuspended in solution D on a tilting table for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... and added to appropriate volumes of buffer RLT and homogenised at 50 Hz for 5 minutes using the TissueLyser LT (Qiagen), until all tissue was homogenised ...
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bioRxiv - Genomics 2023Quote: ... 1µM Template-Switching Oligo (5′-AAGCAGTGGTATCAACGCAGAGTACrGrG+G-3′, where “r” prefixes a ribonucleic acid base and “+” prefixes a locked nucleic acid base, Qiagen) then incubated using a ThermoMixer C with the following conditions ...
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bioRxiv - Genomics 2023Quote: ... The bead-bound gDNA was isothermally amplified for 3 hours at 30 °C then 10 minutes at 65 °C using a miniaturised (1/5 vols) Repli-g Single-Cell assay (Qiagen). The amplified gDNA was cleaned up with 0.8 × vols Ampure XP and 80 % ethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh 5 mM DTT) and incubated for 45 minutes at 65°C before addition of 40 μg Proteinase K (Qiagen) and further incubation for 1.5 hours at 37°C ...
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bioRxiv - Genetics 2023Quote: ... To prepare the protein column we loaded 2 mL (1 mL column volume) of Nickel NTA resin onto the 5 mL Polypropylene columns from Qiagen and the resin buffer was allowed to drain ...
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bioRxiv - Immunology 2023Quote: ... MRC-5/hTERT or A549 cells were reverse transfected with ON-TARGETplus siRNA SMARTpools (Horizon Discovery) or FlexiTube siRNA (Qiagen) using Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: Aliquots of ∼500,000 isolated B cells were pelleted at 400g for 5 minutes and then resuspended in 350uL RLT Plus buffer (Qiagen). Total RNA was then isolated using RNeasy Mini Plus kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Approximately 50 mg of tissue was homogenised in 500 µl phosphate-buffered saline (PBS) for 2 min at 30 Hz using 5 mm steel beads on a TissueLyser II instrument (Qiagen). Total nucleic acids were extracted using the Nucleo Mag Vet Kit (Macherey & Nagel ...
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bioRxiv - Immunology 2024Quote: Samples were double-sorted using a FACSAria or FACS Astrios: 600-1000 cells from each population were collected into 5 μl TCL buffer (Qiagen) containing 1% beta-mercaptoethanol (β-ME ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was thoroughly aspirated and replaced with 20 µl of 1x PBS containing 5 mg/ml of RNase A (Qiagen) and 12 mM of purified H6-mNeonGreen-NLS protein as a loading control ...
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bioRxiv - Physiology 2024Quote: ... was mechanically homogenised in TRI reagent with a 5 mm steel bead at 30 Hz for 2 x 30 s (TissueLyser II, Qiagen) then centrifuged for 10 min at 10,000 g ...
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bioRxiv - Microbiology 2022Quote: ... We performed purification between the two PCRs to reduce contaminants or carry-over of primer dimers using 96-well PCR purification plates (Qiagen Inc., Germantown, MD, USA). The final libraries were quantified with a Qubit Fluorometer (ThermoFischer ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was purified (RNeasy® Mini Kit - Qiagen), according to manufacturer’s instructions and the quality of the RNA was assayed using the Agilent Bioanalyser 2100 (Agilent technologies) ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was isolated using an RNAeasy Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: Total RNA extracted by RNeasy Plant Mini Kit (Qiagen). For synthesis of cDNA SuperScript II reverse transcriptase (18064014 ...
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bioRxiv - Plant Biology 2020Quote: ... and cleansed using the RNeasy Plant Mini Kit (Qiagen). RNA quality and concentration of each sample were checked using an Agilent RNA 6000 Nano Kit Bioanalyzer chip (Agilent Technologies) ...
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bioRxiv - Plant Biology 2020Quote: ... DNA was extracted using DNeasy Plant Mini Kit (Qiagen). Genomes sequence were obtained on an Illumina Hi-Seq 4000 sequencer ...
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bioRxiv - Plant Biology 2019Quote: ... and RNeasy Plant Mini kit (Qiagen, Germantown, MD, USA) was used to isolate and enrich for bacterial RNAs from the samples ...
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bioRxiv - Plant Biology 2019Quote: Total RNA was extracted using a RNeasy kit (QIAGEN). Quantitative RT-PCR was carried out using a PCR system (StepOne ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: Total RNA was extracted using the miRNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted using the RNeasy kit (QIAGEN). Reverse transcription was performed using the SuperScript II Reverse Transcriptase kit (Thermofisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was extracted using the RNeasy Mini Kit (Qiagen). Illumina sequencing libraries were prepared using the Truseq RNA stranded polyA kit (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated with RNeasy mini kit (Qiagen). First strand cDNA was generated with an iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was extracted using the RNeasy Isolation Kit (Qiagen) and converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Systems Biology 2020Quote: ... Total RNA was extracted by RNeasy Mini kit (Qiagen). RNA was then transcribed to cDNA using Superscript II reverse transcriptase based on the manufacture’s protocol (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated using an RNeasy Kit (QIAGEN) using an on-column DNase digestion (RNase-Free DNase Set ...
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bioRxiv - Molecular Biology 2020Quote: ... and repurified using the miRNeasy micro plus Kit (Qiagen). Total RNA and library integrity were verified on LabChip Gx Touch 24 (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted using RNeasy FFPE Kit (Qiagen), RNeasy Mini kit (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... reverse-transcribed into cDNA using Sensiscript RT kit (Qiagen), and pre-amplified using TaqMan PreAmp Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... were isolated using the RNeasy Mini kit (Qiagen GmBH), and then cDNA was prepared with T100 Thermal Cycler (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was purified using MinElute PCR purification kit (QIAGEN). DNA samples were used to measure occupancy of each protein across genes using gene-specific primer sets (Table S1 ...
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bioRxiv - Cell Biology 2020Quote: RNA was isolated using a RNeasy Mini Kit (Qiagen) with DNase treatment as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted using the RNeasy kit (Qiagen) and treated with the DNase I from the DNA-free™ kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was isolated using the RNeasy Micro kit (Qiagen) and reverse transcribed using the SuperScript III kit (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: RNA was extracted using the RNeasy Mini kit (Qiagen). Exogenous ERCC RNA Spike-In Mix 1 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was purified using the RNeasy kit (Qiagen). From each condition ...
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bioRxiv - Immunology 2021Quote: RNA was extracted with the RNeasy mini kit (Qiagen) per the manufacturer’s protocol High-Capacity cDNA Reverse Transcription Kit was used to make cDNA ...
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Microplastic consumption induces inflammatory signatures in the colon and prolongs a viral arthritisbioRxiv - Immunology 2021Quote: ... followed by purification using RNeasy MinElute Cleanup Kit (QIAGEN) as per manufacturer instructions ...
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bioRxiv - Genetics 2021Quote: ... and the RNeasy mini kit (QIAgen, cat. no. 74104), or the Lexogen SPLIT kit (Lexogen ...
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bioRxiv - Genetics 2021Quote: ... samples were purified using a minElute kit (Qiagen, 28006), eluting in 10 μL ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was extracted using miRNeasy Mini kit (Qiagen). RNA integrity was determined using a 4200 TapeStation RNA ScreenTape (Agilent) ...
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bioRxiv - Genetics 2021Quote: ... and purified using RNeasy MinElute clean up kit (Qiagen). Reverse transcription was performed using UMI-primers ...
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bioRxiv - Genetics 2021Quote: ... from a cross with the RNeasy Mini Kit (Qiagen) and reverse transcribed into cDNAs using the M-MLV Reverse Transcriptase kit (ThermoFisher) ...