Labshake search
Citations for Qiagen :
4151 - 4200 of 10000+ citations for Human H ACA Ribonucleoprotein Complex Subunit 1 GAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Triparma retinervis and ‘Scaly parma’) was extracted using the DNeasy Plant Mini Kit or RNeasy Plant Mini Kit (Qiagen, Venlo, Netherlands), respectively ...
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bioRxiv - Physiology 2022Quote: Total RNA was isolated at the indicated time points and cDNA was synthetized using the RNeasy Mini Kit and the QuantiTect™ Reverse Transcription Kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Skin and intestinal samples were handled aseptically and skin-associated DNA was extracted using a NucleoSpin Soil Extraction Kit (Machery-Nagel GmbH, Düren, Germany) while the intestine-associated DNA was extracted using MoBio DNA Extraction Kit (QIAGEN, Hilden, Germany) both as described (Hamilton et al. ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... for 3 h at 37°C under 5% CO2 and total cellular RNA was purified using PureLink™ RNA kit (Life Technology) or RNeasy Mini Kit (Qiagen) and the concentration and RNA Integrity Number (RIN ...
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bioRxiv - Molecular Biology 2023Quote: ... and the RNA-containing aqueous phase was subjected to a column purification kit using RNeasy Mini RNA extraction kit (Qiagen, USA). RNA was DNase treated ...
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bioRxiv - Genetics 2023Quote: Clones obtained from three independent conjugation assays were collected and subjected to genomic extraction using the kit DNeasy® Tissue Kit (Qiagen). DNA was mechanically fragmented using the Covaris method (DNA shearing with sonication) ...
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bioRxiv - Microbiology 2023Quote: Genomic bacterial DNA was isolated from overnight grown cultures (LB, 37°C) by the DNA Extraction kit (DNeasy Kit, Qiagen Germany) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: QIAamp DNA Blood Mini Kit (for whole blood, buffy coat and plasma) and QIAamp DNA Mini Kit for lesion scraping (Qiagen, Germany) were used for DNA extraction as per the manufacturer’s protocols except elution in 50μl elution buffer AE (10 mM Tris-Cl ...
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bioRxiv - Developmental Biology 2023Quote: ... From tissues and clotted blood samples DNA was extracted manually using QiaAmp DNA Blood Midi Kit and the DNeasy Tissue Kit according to manufacturer’s protocol (Qiagen, Valencia, CA). DNA from BA10 was extracted on an automated nucleic acid extraction platform Anaprep (Biochain ...
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bioRxiv - Immunology 2023Quote: The DNA/RNA samples were isolated using either the AllPrep DNA/RNA Mini Kit or the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Then the DNeasy PowerPlant Pro Kit protocol was followed and the extracted DNA was further purified with DNeasy PowerClean CleanUp Kit (Qiagen, Germany).
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was extracted from the thoracic muscle tissue of each individual using either the QIAamp 96 DNA Blood Kit or the DNeasy Blood & Tissue Kits (Qiagen, LLC). Libraries were prepared for low-coverage whole genome sequencing (2×150 bp reads ...
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bioRxiv - Microbiology 2023Quote: ... gene in lung tissues were determined using 2019-nCoV RUO Kit (Integrated DNA Technologies #10006713 and QuantiNova SYBR Green PCR kit (Qiagen #208052) according to the following cycling program ...
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bioRxiv - Cancer Biology 2024Quote: The DNA/RNA samples were isolated using either the AllPrep DNA/RNA Mini Kit or the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The DNA was extracted directly from the frozen samples using a commercial kit (DNeasy Power Soil Pro Kit, QIAGEN, Hilden, Germany), following the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was extracted for total RNA sequencing using the Direct-zol RNA Miniprep Kit (ZymoResearch R2050) or the miRneasy Mini Kit (Qiagen 217004) according to manufacturer protocols ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was extracted using either a QIAGEN HiSpeed® Plasmid Midi Kit or Maxi Kit (QIAGEN Sciences, Germantown, MD, USA) as per the manufacturer’s instructions (HiSpeed® Plasmid Purification Handbook) ...
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bioRxiv - Immunology 2024Quote: ... Astrocytes were isolated using the Anti-ACSA-2 MicroBead Kit (Miltenyi, #130-097-678) and RNA extracted using RNeasy Micro Kit (Qiagen, # 74004). RNA library preparation ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293 cells were transfected with 1:1:2 μg of packaging plasmids versus shRNA hairpins on the pLKO.1 vector using Effectene transfection reagent (Qiagen) 48 h prior to harvesting supernatants ...
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bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 μg DNA ratio using Effectene transfection reagent (Qiagen). Recombinant luciferase-expressing viruses capable of a single round of replication were released into the cell medium and were harvested 48 h later ...
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bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 µg DNA ratio using effectene transfection reagent (Qiagen). Recombinant ...
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bioRxiv - Microbiology 2023Quote: ... 5.0 x105 293T cells were co-transfected with HIV-1 Env-expressing and HIV-1 Tat-expressing plasmids at a ratio of 1:6 using Effectene (Qiagen) and incubated for 48 hours ...
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bioRxiv - Genomics 2021Quote: ... the detection of N-gene of SARS-CoV-2 was performed by using the 2019-nCoV-2 RUO kit (Integrated DNA Technologies, Inc., Coralville, Iowa, USA) and One-Step RT-PCR Kit (QIAGEN® GmbH) on a Rotor-Gene Q real-time PCR cycler (QIAGEN® GmbH) ...
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bioRxiv - Immunology 2021Quote: ... Total RNA from the cells and EVs were first isolated and purified using an RNeasy Mini Kit and a miRNeasy Serum/Plasma kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Genomics 2019Quote: Joint RNA/DNA extractions were performed with the RNA PowerSoil Total RNA Isolation Kit combined with the RNeasy PowerSoil DNA elution kit (MO BIO Laboratories, Inc.; Qiagen, Hilden, Germany). About 5 g of sediment were used ...
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bioRxiv - Pathology 2019Quote: Taxonomy filtered from samples was determined by analysis of kit controls with no template and zymo sequencing controls of know diversity and abundance (the QIAamp DNA Microbiome Kit (Qiagen, Hilden, Germany) DNA Clean and Concentrator Kit (Zymo Research) ...
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bioRxiv - Neuroscience 2019Quote: ... and total RNA was isolated by using the RNeasy Lipid Tissue Mini Kit or RNeasy Plus Universal Mini Kit (Qiagen, Hilgen, Germany). Complementary DNA was synthesized by using the Omniscript RT Kit (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA and genomic DNA were extracted using, respectively, the InviTrap® Spin Universal RNA Mini Kit (Stratec Biomedical, Germany) and the DNeasy Blood & Tissue Kits (Qiagen, Germany) according to manufacturers’ instruction ...
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bioRxiv - Immunology 2021Quote: Total RNA from D+ and D- allografts or kidneys of MCMV infected- and uninfected-BALB/c mice was isolated and purified using the RNeasy Mini Kit and RNeasy MinElute Cleanup kit (Qiagen, Germantown MD) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA was extracted from clarified cell culture supernatants (16,000 g x 10 min) and from infected cells using QIAamp Viral RNA Mini Kit and RNeasy Plus mini kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Genetics 2020Quote: ... iris tissues were extracted using the Precellys 24 homogenizer and lysing kit (Bertin, Montigny-le-Bretonneux, France) together with the AllPrep DNA/RNA kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions including an on-column DNaseI digestion step using the RNase-free DNase Set (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted and purified as described in [9] using the Quick-RNA MiniPrep Kit (Zymoresearch, Irvine, CA, USA) and RNeasy MinElute Cleanup Kit (QIAGEN, Hilden, Germany). Per sample at least 150 ng of high-quality RNA were obtained ...
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bioRxiv - Molecular Biology 2021Quote: ... we extracted RNA using the RNeasy PowerSoil Total RNA Kit and DNA using the RNeasy PowerSoil DNA Elution Kit (Qiagen, Hilden, Germany). On each day of extraction (approximately every 5-15 samples) ...
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bioRxiv - Microbiology 2022Quote: Total RNA samples were extracted from swab samples and tissue samples from the macaques using the QIAamp Viral RNA Mini Kit and RNeasy Plus Mini Kit (Qiagen, Venlo, Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: RNAseq: mRNA was extracted from the iPSCs of the pig-tailed macaque using RNeasy Mini Kit extraction kit (Qiagen Inc. Valencia CA). Following cDNA synthesis ...
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bioRxiv - Genetics 2021Quote: ... and that of cultured cells was obtained using the AllPrep DNA/RNA/miRNA Universal kit or the miRNeasy mini kit (QIAGEN, Hilden, Germany). The samples were reverse-transcribed into cDNA with the High-Capacity cDNA reverse transcription kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... and the sediments were used to coextract the total RNA and DNA using the RNeasy PowerSoil Total RNA isolation kit and the DNA elution kit according to the manufacturer’s protocol (Qiagen®, Valencia, CA). To eliminate potential contaminant DNA in RNA samples ...
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bioRxiv - Bioengineering 2022Quote: ... Total RNA from the cells and EVs was isolated and purified using an RNeasy Mini Kit and a miRNeasy Serum/Plasma kit (Qiagen, Hilden, Germany), respectively ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... was extracted from whole cell RNA extracts (total RNA) using the miRNeasy Mini Kit and the RNeasy MiniElute Cleanup Kit (both Qiagen, Hilden, Germany). Extraction was performed according to the manufactureŕs instructions.
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bioRxiv - Immunology 2024Quote: ... and colon (n = 35 each) and negative blank DNA extraction kit controls using the DNeasy PowerLyzer Powersoil kit (Qiagen, Germantown, MD, USA), with minor modifications to the manufacturer’s protocol as previously described51–53 ...
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bioRxiv - Genomics 2023Quote: ... The bacteriomes of two individuals were dissected and total DNA was extracted using a commercial extraction kit (DNeasy Blood & Tissue Kit, Qiagen, Hilden, Germany), following the manufacturer’s instructions for purification of total DNA from animal tissues ...
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bioRxiv - Microbiology 2023Quote: ... DNA of the cell pellet was extracted using the Invisorb Spin Universal Kit (Invitek Molecular GmbH, Berlin, Germany) and RNA was extracted using the QIAamp RNA Blood Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... followed by genomic DNA removal and cleaning using Qiagen RNase-Free DNase Set kit (cat#79254) and Qiagen Mini RNeasy™ kit (cat#74104) (Qiagen, Denmark). Integrity of the RNA samples was assessed using the Alegient 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... Clarified cell lysate was processed using a Qiagen RNeasy kit and in-column treatment with a DNase I kit (Qiagen, Germantown, MD) for purification of total RNA ...
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bioRxiv - Cell Biology 2023Quote: ... DNA from samples was extracted as per manufacturer’s protocol (Qiagen, DNeasy Blood and Tissue Kit 69504 and Qiagen Genomic DNA miniPrep Kit) and SNP array derived genotypes generated by Affymetrix UK Biobank AxiomTM Array kit by Cambridge Genomic Services (CGS) ...
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bioRxiv - Genomics 2022Quote: Total RNA was extracted from Min6 cells using RNeasy Plus mini kit and from pancreatic islets using RNeasy Plus micro kit (Qiagen, ref 74034) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... Two different kits were used for this method depending on the primer set: Rotor-Gene SYBR® Green RT-PCR Kit (Qiagen, 204174) and SensiFAST™ SYBR® No-ROX One-Step Kit (BioLine ...
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bioRxiv - Microbiology 2023Quote: ... The MDA reaction was performed using the Qiagen REPLI-g Mini kit and purified with the QIAamp DNA Mini kit (Qiagen, Hilden, Germany). Next ...
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bioRxiv - Neuroscience 2024Quote: Total RNAs and miRNAs were extracted from the hippocampal tissue using the RNeasy Mini Kit and the miRNeasy Micro Kit (Qiagen Inc., USA), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic DNA (gDNA) or RNA from the tissues was isolated using a QIAamp DNA mini kit or RNeasy Plus kit (QIAGEN, Germantown, MA). Sequence encoding the modified capsid insertion was amplified using serotype-specific primers with PCR cycles less than 30 by using Q5 2x Master Mix (NEB ...