Labshake search
Citations for Qiagen :
4001 - 4050 of 10000+ citations for Cyclic GMP Direct Chemiluminescent ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... was isolated using Midi or Maxi kits for the validation screens gDNA was isolated using and Midi kits according to the manufacturer’s protocol (Qiagen, Hilden, Germany). PCR and sequencing were performed as previously described56 ...
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bioRxiv - Cancer Biology 2019Quote: DNA was isolated using the QIAamp DNA Blood Mini Kit and the DNeasy Blood & Tissue Kit (both from Qiagen, Hilden, Germany).
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bioRxiv - Physiology 2021Quote: ... cDNA was synthesized using qScriptTM cDNA synthesis kits (QuantaBio, #95047-500) following RNA was isolated from 50- 100mg of sample using RNeasy mini kits (Qiagen, #74106) according to manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: RNAs were extracted from tumor tissues or cancer cell lines then reverse transcribed using Kit from RNAeasy mini kit (Qiagen 74106) and Vazyme (R223-111 01) ...
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bioRxiv - Cell Biology 2022Quote: ... In vitro mRNA transcription was performed from linearized plasmids using the mMessage mMachine SP6 Transcription Kit (Fisher, #AM1340) and purified using the RNeasy Mini Kit (Qiagen, #74104). RNA transcript sizes were confirmed using 1% agarose gels containing 0.05% bleach and Millennium RNA markers (Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was purified from whole individual inner ears using RNA extraction kit and Qiazol Reagent (RNeasy mini kit; Qiagen, Germany). DNase treatment was performed immediately after total RNA purification (RNase-free ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated using the RecoverAll Total Nucleic Acid Isolation Kit (Thermo #AM1975) and concentrated using the RNeasy MinElute Cleanup Kit (Qiagen #74204). Library preparation and sequencing were performed by the Genome Sequencing Facility at St ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples meeting these criteria were macro-dissected from serial unstained sections before extraction of genomic DNA using DNeasy® Blood & Tissue Kits for frozen samples or QIAamp DNA FFPE Tissue Kits for FFPE materials (Qiagen) following the manufacturer instructions.
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bioRxiv - Cancer Biology 2019Quote: DNA was isolated from the remaining pellets after metabolite extraction with a DNA purification kit according to the manufacturer’s protocol (QIAmp DNA Mini Kit for Tissues, QIAGEN, Hilden, Germany). In brief ...
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bioRxiv - Cancer Biology 2019Quote: ... All RNAs for the miRNA investigation were transcribed using miScript RT kits and subsequent qPCR performed using miScript Primer Assays and miScript SYBR kits (Qiagen, Courtaboeuf). Experiments were performed with at least triplicates for each data point ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted from faecal and necropsy samples using the GeneMATRIX stool DNA purification kit (EURx, Poland) and the DNeasy Blood and Tissue kit (Qiagen, Germany), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA samples from liver tissues were extracted by RNeasy Fibrous Tissue Mini Kit and RNeasy Mini Kit following to the manufacturer’s instruction (QIAGEN, Venlo, Netherlands). After quality check by electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... with the NucleoSpin® RNA preparation kit (Macherey–Nagel, Düren) and genomic DNA was quantitatively removed using RNase-Free DNase kit (Qiagen, Hilden) and Ambion™ DNA-free™ DNase kit (Thermo Scientific™ ...
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bioRxiv - Microbiology 2019Quote: ... sterile Dacron swabs (N = 11) and blank DNA extraction kits (N = 23)) using the DNeasy PowerLyzer PowerSoil Kit (Qiagen, Germantown, MD) with minor modifications to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... From old liver tissues and clotted blood samples DNA was extracted manually using QIAamp DNA Blood Midi Kit and the DNeasy Tissue Kit according to manufacturer’s protocol (Qiagen, Valencia, CA). DNA from BA10 was extracted on an automated nucleic acid extraction platform Anaprep (Biochain ...
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bioRxiv - Physiology 2020Quote: ... an equal amount of ethanol was added and loaded on to the kit column and RNA further purified and cleaned using RNeasy kit (QIAGEN, USA) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... From tissues and clotted blood samples DNA was extracted manually using QiaAmp DNA Blood Midi Kit and the DNeasy Tissue Kit according to manufacturer’s protocol (Qiagen, Valencia, CA). DNA from BA10 was extracted on an automated nucleic acid extraction platform Anaprep (Biochain ...
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bioRxiv - Developmental Biology 2020Quote: ... at and after 32-cell stage were extracted using Animal Tissue Direct PCR kit (FOREGENE) and those (~200) of unfertilized eggs and 2-cell embryos were purified using DNeasy® Blood & Tissue Kit (Qiagen). DNA fragments flanking target sites were amplified using primers listed in Supplementary Table S3 ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from peripheral blood of all 395 participants using the genomic DNA extraction kit QIAamp DNA Blood Mini Kit (Qiagen, Germany). The selected genomic DNA regions for the analysis of each gene included the most common reported SNPs (For NAT2 ...
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bioRxiv - Microbiology 2019Quote: ... 10 g of sediment each was used for DNA and RNA isolation with the PowerSoil DNA Isolation Kit (MO BIO) and RNA Powersoil™ Total RNA Isolation Kit (QIAGEN), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... The first replicate was extracted using the ISOLATE II Genomic DNA Kit as described in 2.1 and the second using the DNeasy Blood and Tissue Kit protocol (Qiagen, Valencia, CA) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μg of RNA per sample was reverse transcribed using a complementary DNA (cDNA) synthesis kit (QuantiTect reverse transcription kit, Qiagen, Germany). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2022Quote: ... Triparma retinervis and ‘Scaly parma’) was extracted using the DNeasy Plant Mini Kit or RNeasy Plant Mini Kit (Qiagen, Venlo, Netherlands), respectively ...
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bioRxiv - Physiology 2022Quote: Total RNA was isolated at the indicated time points and cDNA was synthetized using the RNeasy Mini Kit and the QuantiTect™ Reverse Transcription Kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Skin and intestinal samples were handled aseptically and skin-associated DNA was extracted using a NucleoSpin Soil Extraction Kit (Machery-Nagel GmbH, Düren, Germany) while the intestine-associated DNA was extracted using MoBio DNA Extraction Kit (QIAGEN, Hilden, Germany) both as described (Hamilton et al. ...
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bioRxiv - Microbiology 2023Quote: Genomic bacterial DNA was isolated from overnight grown cultures (LB, 37°C) by the DNA Extraction kit (DNeasy Kit, Qiagen Germany) following the manufacturer’s instructions ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... for 3 h at 37°C under 5% CO2 and total cellular RNA was purified using PureLink™ RNA kit (Life Technology) or RNeasy Mini Kit (Qiagen) and the concentration and RNA Integrity Number (RIN ...
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bioRxiv - Molecular Biology 2023Quote: ... and the RNA-containing aqueous phase was subjected to a column purification kit using RNeasy Mini RNA extraction kit (Qiagen, USA). RNA was DNase treated ...
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bioRxiv - Genetics 2023Quote: Clones obtained from three independent conjugation assays were collected and subjected to genomic extraction using the kit DNeasy® Tissue Kit (Qiagen). DNA was mechanically fragmented using the Covaris method (DNA shearing with sonication) ...
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bioRxiv - Immunology 2023Quote: The DNA/RNA samples were isolated using either the AllPrep DNA/RNA Mini Kit or the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: QIAamp DNA Blood Mini Kit (for whole blood, buffy coat and plasma) and QIAamp DNA Mini Kit for lesion scraping (Qiagen, Germany) were used for DNA extraction as per the manufacturer’s protocols except elution in 50μl elution buffer AE (10 mM Tris-Cl ...
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bioRxiv - Developmental Biology 2023Quote: ... From tissues and clotted blood samples DNA was extracted manually using QiaAmp DNA Blood Midi Kit and the DNeasy Tissue Kit according to manufacturer’s protocol (Qiagen, Valencia, CA). DNA from BA10 was extracted on an automated nucleic acid extraction platform Anaprep (Biochain ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Then the DNeasy PowerPlant Pro Kit protocol was followed and the extracted DNA was further purified with DNeasy PowerClean CleanUp Kit (Qiagen, Germany).
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was extracted from the thoracic muscle tissue of each individual using either the QIAamp 96 DNA Blood Kit or the DNeasy Blood & Tissue Kits (Qiagen, LLC). Libraries were prepared for low-coverage whole genome sequencing (2×150 bp reads ...
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bioRxiv - Microbiology 2023Quote: ... gene in lung tissues were determined using 2019-nCoV RUO Kit (Integrated DNA Technologies #10006713 and QuantiNova SYBR Green PCR kit (Qiagen #208052) according to the following cycling program ...
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bioRxiv - Cancer Biology 2024Quote: The DNA/RNA samples were isolated using either the AllPrep DNA/RNA Mini Kit or the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The DNA was extracted directly from the frozen samples using a commercial kit (DNeasy Power Soil Pro Kit, QIAGEN, Hilden, Germany), following the instructions of the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... Astrocytes were isolated using the Anti-ACSA-2 MicroBead Kit (Miltenyi, #130-097-678) and RNA extracted using RNeasy Micro Kit (Qiagen, # 74004). RNA library preparation ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was extracted using either a QIAGEN HiSpeed® Plasmid Midi Kit or Maxi Kit (QIAGEN Sciences, Germantown, MD, USA) as per the manufacturer’s instructions (HiSpeed® Plasmid Purification Handbook) ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293 cells were transfected with 1:1:2 μg of packaging plasmids versus shRNA hairpins on the pLKO.1 vector using Effectene transfection reagent (Qiagen) 48 h prior to harvesting supernatants ...
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bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 μg DNA ratio using Effectene transfection reagent (Qiagen). Recombinant luciferase-expressing viruses capable of a single round of replication were released into the cell medium and were harvested 48 h later ...
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bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 µg DNA ratio using effectene transfection reagent (Qiagen). Recombinant ...
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bioRxiv - Microbiology 2023Quote: ... 5.0 x105 293T cells were co-transfected with HIV-1 Env-expressing and HIV-1 Tat-expressing plasmids at a ratio of 1:6 using Effectene (Qiagen) and incubated for 48 hours ...
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bioRxiv - Genomics 2021Quote: ... the detection of N-gene of SARS-CoV-2 was performed by using the 2019-nCoV-2 RUO kit (Integrated DNA Technologies, Inc., Coralville, Iowa, USA) and One-Step RT-PCR Kit (QIAGEN® GmbH) on a Rotor-Gene Q real-time PCR cycler (QIAGEN® GmbH) ...
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bioRxiv - Immunology 2021Quote: ... Total RNA from the cells and EVs were first isolated and purified using an RNeasy Mini Kit and a miRNeasy Serum/Plasma kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Genomics 2019Quote: Joint RNA/DNA extractions were performed with the RNA PowerSoil Total RNA Isolation Kit combined with the RNeasy PowerSoil DNA elution kit (MO BIO Laboratories, Inc.; Qiagen, Hilden, Germany). About 5 g of sediment were used ...
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bioRxiv - Pathology 2019Quote: Taxonomy filtered from samples was determined by analysis of kit controls with no template and zymo sequencing controls of know diversity and abundance (the QIAamp DNA Microbiome Kit (Qiagen, Hilden, Germany) DNA Clean and Concentrator Kit (Zymo Research) ...
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bioRxiv - Neuroscience 2019Quote: ... and total RNA was isolated by using the RNeasy Lipid Tissue Mini Kit or RNeasy Plus Universal Mini Kit (Qiagen, Hilgen, Germany). Complementary DNA was synthesized by using the Omniscript RT Kit (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA and genomic DNA were extracted using, respectively, the InviTrap® Spin Universal RNA Mini Kit (Stratec Biomedical, Germany) and the DNeasy Blood & Tissue Kits (Qiagen, Germany) according to manufacturers’ instruction ...
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bioRxiv - Immunology 2021Quote: Total RNA from D+ and D- allografts or kidneys of MCMV infected- and uninfected-BALB/c mice was isolated and purified using the RNeasy Mini Kit and RNeasy MinElute Cleanup kit (Qiagen, Germantown MD) according to the manufacturer’s instructions ...