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Citations for Qiagen :
351 - 400 of 2627 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: Cells were lysed using Buffer RLT (Qiagen) and homogenized using either syringes and needles or QIAshredder (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... RLT buffer (Qiagen Kit 74004, DTT added) were added to the tubes ...
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bioRxiv - Cancer Biology 2023Quote: ... and subsequently lysed in RLT buffer (Qiagen). Total RNA was isolated using the RNeasy kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... lysed with 350uL Buffer RLT (Qiagen 79216), scraped off the plate (Fisher 08100241) ...
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bioRxiv - Cancer Biology 2023Quote: ... 519 μl Buffer AL (Qiagen Blood & Tissue) was added ...
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bioRxiv - Developmental Biology 2024Quote: ... resuspended in 350 μl RLT buffer (QIAGEN) and homogenized using a 1 ml syringe with a 21G x 1½” needle ...
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bioRxiv - Immunology 2023Quote: Cells were lysed in RLT buffer (QIAGEN) supplemented with 38 mM DTT ...
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bioRxiv - Physiology 2024Quote: ... lysed in 300µL of RLT buffer (Qiagen) and stored at -80°C until extraction of all samples ...
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bioRxiv - Neuroscience 2024Quote: ... 0.01% Tween-20 in buffer EB (Qiagen)).
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bioRxiv - Molecular Biology 2024Quote: ... Initial dissociation was conducted RLT buffer (Qiagen) supplemented with dithiothreitol ...
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Convergence, plasticity, and tissue residence of regulatory T cell response via TCR repertoire prismbioRxiv - Immunology 2023Quote: ... and lysed in buffer RLT plus (Qiagen) and froze at -80°C until further use ...
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bioRxiv - Immunology 2023Quote: ... ASCs were lysed using RLP buffer (QIAGEN). RNA was extracted using the RNeasy Plus Micro Kit (QIAGEN ...
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bioRxiv - Cell Biology 2020Quote: ... Approximately 60 homozygous males from each E-box deleted fly line were disrupted in lysis-buffer (Buffer AL, Qiagen) using a motorized microtube homogenizer ...
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bioRxiv - Genomics 2022Quote: ... The DNA was eluted from the beads with 50 µL Sample Elution Buffer (Qiagen AE Buffer, 0.1% Tween-20). Qubit dsDNA BR kit was used to quantify the DNA.
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bioRxiv - Neuroscience 2019Quote: ... Pools of 150 RFP+ and RFP− individual cells were laser captured from the granular cell layer of the dentate gyrus of each mouse using a Leica Microsystem LMD system under a HC PL FL L 63/0.60 XT objective and collected in lysis buffer (50 ul RLT buffer, Qiagen). Total RNA from LCM samples was isolated separately from each animal.
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Caspase-8 Modulates Angiogenesis By Regulating A Cell Death Independent Pathway In Endothelial CellsbioRxiv - Developmental Biology 2019Quote: ... The positive cell fraction was eluted from the columns with PEB buffer and directly frozen in RLT buffer (Qiagen) for RNA isolation.
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bioRxiv - Genetics 2019Quote: Single cells meant for RNA processing were sorted into 96-well full-skirted Eppendorf plates that were pre-chilled at 4°C and were prefilled with 10μL of lysis buffer consisting of TCL buffer (Qiagen) supplemented with 1% beta-mercaptoethanol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The samples were centrifuged and the supernatant mixed with 13 mL of a modified PB buffer (12.6 mL PB buffer (Qiagen), 6.5 μL Tween-20 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and 3.5 ml of RLT buffer with B-mercaptoethanol (10 µl for every 350 µl of RLT buffer – Qiagen) was added ...
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bioRxiv - Physiology 2019Quote: ... The denatured and washed PCR products were then placed in annealing buffer (Pyromark annealing & wash buffers, Qiagen, Manchester UK) with 0.5 μM sequencing primer at 75°C for two minutes on a heating block and the block then turned off for 10 minutes with the samples left in the block and then a further 10-15 minutes to cool at room temperature ...
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bioRxiv - Cell Biology 2020Quote: For both HSC and MK isolation cells were sorted into wells each containing 2.5ul lysis buffer/well: 950uL RLT Plus buffer (Qiagen) and 50ul 20U/ul SUPERase In RNase inhibitor (Ambion ...
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bioRxiv - Immunology 2020Quote: ... which contained 5 μl of sort buffer consisting of (1X Qiagen One-step RT PCR Buffer (Qiagen, Hilden, Germany), 0.1 mM dithiothreitol (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and sorted into wells of a 96-well plate (185 nuclei/well) containing reverse cross-linking buffer (11µL EB buffer (Qiagen) 0.5µL Proteinase K (Roche) ...
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bioRxiv - Immunology 2023Quote: ... and HFD-C-fed Stat4fl/flLdlr-/- and Stat4ΔLysMLdlr-/- mice were isolated using negative selection (StemCell) and immediately resuspended in lysis buffer (Buffer RLT, Qiagen) supplemented with 1% 2-mercaptoethanol for RNase inhibition ...
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bioRxiv - Cell Biology 2023Quote: ... individual organoids were washed 2 times with ice-cold PBS and subsequently dissociated with 350µL of lysis buffer (RLT Buffer, QIAGEN). Samples were stored at - 80°C until they were delivered to USEQ Utrecht Sequencing Facility (Utrecht ...
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bioRxiv - Genomics 2024Quote: ... Homogenization buffer for RNA purification was made by adding 1:100 beta-mercaptoethanol to Buffer RLT (Qiagen, Valencia, CA) and kept on ice until use ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The concentration of the resulting His-tagged ß-CATENIN-1 fragments was quantified via quantitative Western blotting with a Penta-His antibody (Qiagen) and a recombinant His-tagged protein as external standard.
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bioRxiv - Cell Biology 2020Quote: ... For each replicate about 60 wing discs were dissected from wandering larvae and immediately transferred to the lysis buffer (Buffer RLT, Qiagen) supplemented with β-mercaptoethanol (1:100 ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 50 mM EDTA and 50 mM EGTA to the NHS beads or Modified RLT buffer (1x Buffer RLT supplied by Qiagen, 10mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2019Quote: ... 6µL ethanol/phosphate buffer (85:15 v/v ethanol/10mM phosphate buffer) were added and the tissue was homogenized (TissueLyser II, Qiagen). Samples were centrifuged at 5000g for 5 minutes at 4°C and the supernatants were transferred to a fresh tube and used for analyses ...
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bioRxiv - Genomics 2020Quote: ... The 96-well plates contained 12μl per well of reverse cross-linking buffer (0.83 mg/ml Proteinase K and 0.042% SDS in Qiagen EB buffer), and were put on ice ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The solidified plugs were submerged in Lysis Buffer solution (Bionano) and Proteinase K (Puregene/Qiagen, 66.8 µl per ml Buffer) for two hours at 50°C ...
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bioRxiv - Plant Biology 2021Quote: ... Fluorescence positive cells were collected in a round-bottom polystylene tube that contains 150 - 200 μl RLT buffer with beta-mercaptoethanol (10 μl / 1 ml RLT buffer, RNeasy micro kit protocol, Qiagen). Cell sorting was performed for about 15 min and collected cells were immediately frozen on dry ice and kept in −80 °C for up to one week ...
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bioRxiv - Immunology 2020Quote: Single cells were sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, Qiagen 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: ... buffer with lysozyme (1mg/mL) and incubated at RT for 2 min prior to adding 700 μL RLT Buffer (Qiagen) with 10ul/mL B-mercaptoethanol (Sigma) ...
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The mitochondrial genome of the red icefish (Channichthys rugosus) casts doubt on its species statusbioRxiv - Zoology 2022Quote: A small piece of muscle tissue (5.8 mg dry weight) was dried in a vacuum centrifuge and immersed in lysis buffer (260 μL ATL buffer (Qiagen) and 40 μL Proteinase K [20 mg/mL]) ...
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bioRxiv - Biochemistry 2020Quote: ... resuspended in buffer A supplemented with 8 M guanidine hydrochloride (= buffer B) and loaded onto an Ni-NTA agarose column (QIAGEN) pre-equilibrated in the same buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... but we used 20µL of heated elution buffer (Buffer AE) from the Qiagen DNeasy Blood & Tissue Kit to maximize DNA yield (Qiagen 69506). Once DNA was isolated ...
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Glutaric aciduria type 3 is a naturally occurring biochemical trait in inbred mice of 129 substrainsbioRxiv - Biochemistry 2020Quote: ... were homogenized in ice-cold RIPA buffer supplemented with protease inhibitor cocktail (∼20 mg tissue per 1 mL lysis buffer) using a TissueLyser II apparatus (Qiagen) and the homogenates were centrifuged at 1,000xg ...
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bioRxiv - Genomics 2021Quote: ... were sorted into individual wells of a 96 well plate containing lysis buffer 2.5μL RLT Plus Lysis Buffer (QIAGEN, catalog # 1053393) with 1U/μL SUPERase-In (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The samples were purified using 500 μl PB and 650 μl of PE buffer and eluted in 30 μl EB buffer (MinElute PCR Purification Kit, QIAGEN). The adaptor ligation module was implemented using 10 μl of buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The Bacmid DNA pellet was then dried for 1 minute and re-suspended in 50 μL Buffer EB (10 mM Tris-Cl, pH 8.5; All buffers from QIAprep Spin Miniprep Kit, Qiagen #27104). Purified bacmid was then transfected into Sf9 cells ...
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bioRxiv - Cancer Biology 2021Quote: The frozen tumors were minced to small pieces and suspended in buffer P1 (QIAGEN, 1 mL Buffer/100 mg tumor) supplemented with 100 μg/mL RNase A (Promega) ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... and CD45+ Calcein+ DAPI-CD19+ CD38+ activated B cells were single-cell sorted into 96-well plates with catching buffer (RLT lysis buffer (Qiagen) with 1% 2-mercaptoethanol (Sigma-Aldrich)) ...
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bioRxiv - Genomics 2020Quote: ... 25 nuclei were sorted into each well of 96-well plates (8-10 plates per experiment) containing 12 µl of nuclear lysis buffer (11 µl of EB buffer (Qiagen) supplemented with 0.5 µl of 100X BSA and 0.5 µl of 1% SDS) ...
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bioRxiv - Immunology 2020Quote: ... in 96 flat bottom plates in the presence or absence of PMA (200ng/ml) and ionomycin (300ng/ml) were lysed in 100Sµl lysis buffer containing TCL buffer (Qiagen, 1031576) containing 1% β-mercaptoethanol ...
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bioRxiv - Immunology 2021Quote: Single cells were index-sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, QIAGEN 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was then air dried for 1 minute and re-suspended in 50 μL Buffer EB (10 mM Tris-Cl, pH 8.5; All buffers from QIAprep Spin Miniprep Kit, Qiagen #27104). Purified bacmid was then transfected into Sf9 cells ...
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bioRxiv - Microbiology 2022Quote: ... the beads were suspended in Buffer D2 (denaturation buffer) and subjected to MDA reaction using a REPLI-g Single Cell Kit (QIAGEN).
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bioRxiv - Microbiology 2023Quote: ... Cells were sorted into 96-well plates containing lysis buffer with proteinase K solution in PKD buffer (1:16) (Qiagen) and were stored at -80ºC ...