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Citations for Qiagen :
351 - 400 of 463 citations for Urotensin I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: RNA was extracted using the RNeasy Plant Mini Kit with in-column DNase I digestion (Qiagen). Five hundred ng total RNA was reverse transcribed using oligo(dT ...
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bioRxiv - Immunology 2020Quote: ... following the manufacturer’s instructions and including the digestion of genomic DNA with DNAse I (79254, Qiagen). The cells were lysed in RLT buffer (Qiagen) ...
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The C. elegans RIG-I homolog drh-1 mediates the Intracellular Pathogen Response upon viral infectionbioRxiv - Microbiology 2019Quote: ... and additionally purified using the RNeasy clean-up kit with on-column DNase I digestion (Qiagen). RNA quality was assessed by TapeStation at the UC San Diego Institute for Genomic Medicine ...
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bioRxiv - Genomics 2021Quote: ... contaminating DNA was removed through on-column digestion using the RNase-free DNase I set (Qiagen). RNA purity was assessed using the NanoDrop 6000 ...
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bioRxiv - Microbiology 2021Quote: ... and residual genomic DNA was removed from RNA samples by on-column DNAse I treatment (Qiagen). Both steps were performed according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated and DNAse I digested using RNEasy Plant Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The transcription factor prediction was performed using i-cisTarget20 and the upstream regulator function by QIAGEN Ingenuity Pathway Analysis.21 Differentially expressed genes (DEGs ...
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bioRxiv - Cell Biology 2022Quote: ... per μg of RNA was used followed by an on-column treatment with DNase I (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated and DNase I treated using the RNeasy Plus Mini Kit from Qiagen. RNA integrity was checked by Agilent Bioanalyzer 2100 or Agilent Tapestation 4200 and samples with clean rRNA peaks (RIN>7 ...
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bioRxiv - Microbiology 2023Quote: ... RNA were extracted using an RNeasy Mini-Kit from Qiagen and following the manufacturer’s protocol and including a DNase I (RNase-free DNases set from Qiagen) treatment ...
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bioRxiv - Plant Biology 2024Quote: ... Removal of the DNA from the samples was carried out through a DNase I treatment (QIAGEN). The cDNA was then synthesized with an Omniscript Reverse Transcription (RT ...
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bioRxiv - Systems Biology 2020Quote: ... and RNA was then subjected to DNase I digestion and purified using a miRNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... and total RNA was extracted with QIACube using RNeasy kit with on-column DNase I treatment (Qiagen). For each sample ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... instrument according to the manufacturer’s protocol with on column for RNase-Free DNase I (Qiagen, Cat# 79254) treatment ...
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bioRxiv - Synthetic Biology 2019Quote: ... according to the manufacturer’s protocols including the on-column DNA digestion (RNase-free DNase I, Qiagen, 79254). RNA was reverse transcribed using either the QuantiTect Reverse Transcription Kit (Qiagen ...
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A novel transplantable model of lung cancer associated tissue loss and disrupted muscle regenerationbioRxiv - Cancer Biology 2020Quote: ... and DNase I treated using Trizol and purified on RNeasy Mini kit columns (Qiagen, Mississauga, ON, Canada). RNA was quantified using a NanoDrop Spectrophotometer (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: ... with on-column DNase I digestion to remove genomic DNA using RNase-Free DNase Set (#79254, QIAGEN). The concentration of RNA was determined by Nanodrop 2000 ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted with Trizol followed by DNAse I treatment and purification (RNeasy RNA purification kit,Qiagen).The purified RNA was quantified with Nanodrop and QC checked by Agilent Bioanalyzer.
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bioRxiv - Cancer Biology 2022Quote: ... according to the manufacturer’s instructions and treated with DNase I by using RNase free DNase set (Qiagen).
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bioRxiv - Microbiology 2022Quote: ... DNA extraction was performed on each piece using the following methods: (i) DNeasy Blood & Tissue kit (Qiagen, Hilden ...
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bioRxiv - Cell Biology 2022Quote: ... Condensin I was bound to Glutathione Sepharose 4B beads (Cytiva) in a 5 mL polypropylene column (Qiagen) using GST- tagged mSMC4 and eluted by PreScission Protease (Cytiva ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was purified and DNAse I treated according to the manufacturer’s instructions using RNeasy mini kits (Qiagen). For embryo inhibitor experiments ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time PCR was performed using SYBR Green I fluorescence and Rotor-Gene Q cycler (Qiagen). Melting-curve analysis was done using Rotor-Gene Q series software version 2.1.0.
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bioRxiv - Immunology 2020Quote: Total RNA from ~300,000 OT-I cells per sample was collected with the RNeasy micro kit (Qiagen) with a 15-minute on-column DNase digestion (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... and 7 were isolated using a Plant RNeasy Mini kit with DNase I treatment (Qiagen, Hilden, Germany). Three biological replicates were prepared for each tissue type ...
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bioRxiv - Immunology 2021Quote: DNase I-treated total RNA was prepared from sorted T cells using RNeasy RNA isolation kit (Qiagen) and cDNA was synthesized using an iScript cDNA synthesis kit (Biorad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was isolated and DNase I treated using Qiagen RNeasy Plus Mini Kit (Qiagen, Valencia, CA). The RNA integrity was checked by Agilent Bioanalyzer 2100 (Agilent ...
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bioRxiv - Developmental Biology 2022Quote: ... In vitro transcribed mRNAs were treated with DNase I and purified using the RNeasy Mini kit (Qiagen). EnGen Spy Cas9 NLS protein (NEB ...
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bioRxiv - Genomics 2022Quote: ... DNA from I-73-1 and D308 was extracted using a ‘Genomic-tip 100/G’ kit (Qiagen) and long-reads sequenced with PacBio RS II at 100x coverage ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA contamination was removed by treating the isolated RNA samples with RNase-free DNase I (Qiagen), followed by phenol/chloroform extraction and ethanol precipitation ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... according to the manufacturer’s instructions in which we performed the optional DNAse I (Qiagen, Valencia, CA, USA) incubation step ...
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bioRxiv - Microbiology 2023Quote: ... A total of 384 different conditions were included in the screen (JCSG Core Suite I-IV, Qiagen) and crystals obtained in 0.2 M disodium tartrate supplemented with 20% (w/v ...
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bioRxiv - Developmental Biology 2023Quote: ... extraction followed by clean up and on-column DNase I treatment using an RNeasy Mini Kit (Qiagen). cDNA was reverse transcribed using SuperScript III (Thermo Fisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... remaining cellular DNA was digested using 0.0164 Kunitz Units/µL DNase I in Buffer RDD (79254, Qiagen), RNA digestion was prevented by utilizing 0.533 U/µL RNasin® ribonuclease inhibitor (N2511 ...
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bioRxiv - Plant Biology 2024Quote: ... using the RNeasy Plant Mini with DNase I treatment according to the manufacturer’s instructions (Qiagen, Valencia, CA). One μg of RNA was used for a 20-µL first-strand cDNA synthesis reaction using High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: RNA was purified using the PureLink RNA mini kit with in-column DNAse-I digest (#79254, Qiagen) following the manufacturer’s instructions (#12183018A ...
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bioRxiv - Plant Biology 2020Quote: ... The DNA in each total RNA sample was digested using DNase I (RNase-free DNase sets, Qiagen, CA). The RNA-seq library was prepared through the Lasy-Seq v1.1 protocol (Kamitani et al. ...
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bioRxiv - Molecular Biology 2021Quote: qPCR on cDNA and ChIP samples was carried out in reactions containing SYBR Green I HotStarTaq enzyme (Qiagen). Relative quantitation was performed using standard curves made by pooling aliquots of each cDNA sample being examined ...
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bioRxiv - Genomics 2020Quote: ... including RNase I and Proteinase K treatment followed by DNA clean up using the PCR purification kit (Qiagen). Samples were labelled with cy3 using a One-Colour Labeling Kit (Roche-Nimblegen) ...
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bioRxiv - Pathology 2019Quote: ... RNA extraction was performed according to the “Animal Cell I” protocol from a RNeasy Mini kit (Qiagen, USA). cDNA synthesis was performed with a 1 μg RNA sample using the First Strand cDNA Synthesis kit RT-PCR (Roche ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was extracted and DNase I-treated from 106 cell pellets using the RNeasy Mini kit (Qiagen). RNA was reverse-transcribed using SuperScript III (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... with a rotor stator homogenizer (VMR) and put through an “on column” DNAse digestion using DNAse I (Qiagen). RNA concentration was determined by a Nanodrop spectrophotometer (GE Healthcare 28 9244-02 ...
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bioRxiv - Immunology 2020Quote: ... MuSK1A crystals were grown at 18 °C by vapor diffusion and after ≈ 4 days grew from drops consisting of 100 nL protein plus 100 nL of a reservoir solution consisting of 20% w/v PEG 6K (Precipitant) and 0.1 M BICINE 8.5 pH (Buffer) from the JCSG Core I (Qiagen) screen ...
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bioRxiv - Genomics 2022Quote: ... Between 50 and 75 ug of nucleic acids were treated with 6.8 units of DNase I using manufacturer’s buffers and columns (RNase-free DNase set; Qiagen) and 1g of glycogen ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from the seedlings was extracted using the RNeasy Plant Mini Kit with Dnase I digestion (QIAGEN). Reverse transcription was carried out with 500 ng of total RNA using the iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated using TRIzol separation (ThemoFisher) combined with RNeasy column cleanup and DNase I treatment (Qiagen). Samples were diluted to a concentration of 60 ng/µL in nuclease-free water before shipping to the Mayo Clinic Genome Analysis Core ...
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bioRxiv - Biochemistry 2023Quote: ... Screening plates were set up by a sitting-drop vapor diffusion method using JCSG Core I-IV (Qiagen), PEG Suite I-II (Qiagen) ...
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bioRxiv - Developmental Biology 2023Quote: RNA was isolated using the miRNeasy kit with DNase I digestion performed on the column (Qiagen, Venlo, Netherlands). All samples had RIN numbers of 9.9 or 10 when analyzed using the TapeStation System (Agilent Technologies ...
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Cytokinin-promoted secondary growth and nutrient storage in the perennial stem zone of Arabis alpinabioRxiv - Plant Biology 2020Quote: ... including DNase I digestion via the RNase-Free DNase Set to remove traces of genomic DNA (Qiagen, Hilden, Germany). Quality and quantity of the isolated RNA was examined with the Fragment Analyzer (Advanced Analytical Technologies GmbH ...
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bioRxiv - Microbiology 2020Quote: ... and an on-column DNase I digest was performed with the Qiagen RNase-Free DNase Set (Qiagen, Hilden, Germany). RNA was eluted in 50 μl of RNase-free molecular biology grade water and DNA was removed by incubation with 1 μl TURBO DNase (2 U/μl ...