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Citations for Qiagen :
351 - 400 of 1144 citations for Tetanus Toxoid Recombinant Heavy Chain Fragment C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2022Quote: ... The PCR product was isolated from gel fragments with a Gel Extraction Kit (Qiagen, Manchester, UK), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... CDNA fragments were amplified with strain-specific primers using the one step RT-PCR kit (Qiagen). Sequencing was performed by Macrogen Europe ...
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bioRxiv - Molecular Biology 2020Quote: Expression plasmids: PCR fragments of the expected sizes were extracted using a gel extraction kit (Qiagen) and cloned in pGEM-T easy vector (Promega) ...
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bioRxiv - Microbiology 2020Quote: The 3 Kb mutagenic DNA fragments were purified using a QIAquick PCR Purification Kit (Qiagen; 28106) and transformed into V ...
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bioRxiv - Developmental Biology 2019Quote: ... The PCR fragments were gel-purified using the QIAquick Gel Extraction Kit (Qiagen, Cat. No: 28704) and the respective constructs were assembled with BbsI-HF (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The amplified fragments were purified using a QIAquick PCR purification kit (Qiagen Genomics, Bothell, WA, USA) following the manufacturer‘s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR fragments of the expected size were purified using the Qiaquick Gel purification kit protocol (Qiagen). An overhang was added to purified Or67b amplicons with Taq polymerase (Fermentas ...
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bioRxiv - Plant Biology 2022Quote: ... Gel extraction of fragments was performed with the QIAquick gel extraction kit (Qiagen; Cat No.: 28704) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Ribosome-protected fragments (RPFs) were extracted from pelleted 80S ribosomes using a miRNeasy kit (QIAgen, #217004) following the manufacturer’s protocol for enriched recovery of small RNAs (<200 nt) ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments resolved with electrophoresis of 1.2% Agarose gel purified using the Gel Extraction Kit (Qiagen) and subject to Sanger sequencing at Quintara Biosciences.
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bioRxiv - Microbiology 2023Quote: ... The resulting cDNA fragments were purified with the QIAQuick PCR Purification Kit (cat. no. 28104, Qiagen), end-repaired ...
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bioRxiv - Developmental Biology 2023Quote: ... The transposed DNA fragments were purified immediately after with a MinElute PCR Purification Kit (Qiagen, 28004). After samples were PCR-amplified using 1×NEBNext High-Fidelity PCR Master Mix (NEB ...
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bioRxiv - Molecular Biology 2020Quote: Purified recombinant poly-histidine protein was obtained by FPLC under native conditions using a Ni-NTA column (Qiagen), and a Biologic DUO-FLOW BIO-RAD chromatography system (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Preparation of hexahistidine-tagged recombinant DCP1 was performed according to the manufacturer’s instructions in Tris buffer (Qiagen, 30210). The abundance of proteins was estimated by CBB staining by SDS-PAGE or on immunoblots using anti-his antibodies ...
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bioRxiv - Microbiology 2021Quote: The recombinant plasmid DNA derived from positive clones was isolated by using the QIAGEN plasmid mini kit (QIAGEN) and digested by PmlI (vector PFLD ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins NHis6-Fnr1 and Fnr3-CHis6 in the supernatant were purified on Ni2-NTA resin (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... filtered and the secreted recombinant proteins were purified by affinity chromatography using Ni-NTA resin (Qiagen; Cat.# 30230). Specifically ...
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bioRxiv - Microbiology 2022Quote: ... The cells were collected by centrifugation and the recombinant protein was column-purified using Ni2+-NTA resin (Qiagen). The purified protein was stored at -80°C ...
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bioRxiv - Microbiology 2023Quote: ... second and 10th passage in ECE of recombinant NDV_GRABV using the QIAamp® Viral RNA Mini Kit (Qiagen). Genomic regions ...
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bioRxiv - Molecular Biology 2024Quote: ... The agarose gel bound with the His-tagged recombinant protein was packed in the chromatography column (Qiagen, Germany), and the flow through was discarded ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 min, 4°C) prior to incubation (1 h, 4°C) with 4.0 mL of Ni-NTA agarose (Qiagen) pre-equilibrated in the same buffer ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was extracted from plant cells and Hi-C libraries were prepared using EpiTect Hi-C Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... 4°C) and the supernatant incubated 1 hr at 4°C with 1.5 ml packed Ni-NTA Agarose beads (Qiagen). Beads were washed with buffer H adjusted to 50 mM imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... Extract was cleared by centrifugation at 186,000g for 1 hour at 4 °C and then incubated at 4 °C with NiNTA resin (QIAGEN) for 4 h ...
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bioRxiv - Microbiology 2021Quote: ... and the chitinase gene fragment was isolated via gel extraction with the QIAQuick Gel Extraction kit (Qiagen). pGRG36 was also digested with XhoI and XmaI and extracted via gel extraction using the QIAEX II gel extraction kit (Qiagen) ...
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bioRxiv - Developmental Biology 2020Quote: ... After gel-purification of the PCR fragments with the QIAquick Gel Extraction Kit (Qiagen, Cat. No: 28704), these were assembled with BbsI-HF (New England Biolabs ...
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bioRxiv - Genomics 2019Quote: ... Resulted DNA fragments from four independent ChIP assays were purified with QIAquick PCR Purification Kit (QIAGEN #28106). Afterwards ...
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bioRxiv - Bioengineering 2020Quote: ... agarose gel purification of respective fragments was performed using QIAEX II Gel Extraction Kit (QIAGEN, Hilden, Germany) and ligations were performed using pJET 1.2 Cloning Kit or TOPO™ TA Cloning™ (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... frozen fragments of human cortex were homogenized with a motorized hand pestle in RLT lysis buffer (Qiagen) and qPCR was performed as described in qPCR section.
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bioRxiv - Microbiology 2021Quote: ... The three PCR fragments were digested with DpnI and purified with a Qiagen PCR Purification Kit (Qiagen). The three PCR products were assembled as one large fragment (5’ cynX - insert - lacA3’ ...
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bioRxiv - Microbiology 2021Quote: ... The resultant RSUFG9 and RSB2 fragments (392 bp) were purified with the QIAquick PCR purification kit (Qiagen), and subsequently subjected to sequential digestion with XbaI and EcoRV (which also leaves 5’-P blunt ends) ...
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bioRxiv - Molecular Biology 2022Quote: ... The JAK3 PCR fragment was purified and cloned into the pQE-Tri System His-Strep2 vector (Qiagen). The final pQE-JAK3 construct was used to transfect GC using the Xfect transfection kit (Takara Bio ...
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bioRxiv - Genomics 2019Quote: ... The products were enriched for the cDNA fragments using a Qiaquick Gel Extraction Kit (Qiagen, Duesseldorf, Germany) and amplified with polymerase chain reaction (PCR ...
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bioRxiv - Genetics 2021Quote: ... DNA fragments from the input and IP fractions were purified using a PCR purification kit (Qiagen, 28104) and measured with qPCR.
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bioRxiv - Evolutionary Biology 2021Quote: ... fragments between 450 and 700 bp were excised and purified with the MinElute Gel Extraction kit (Qiagen). Indexed Illumina sequencing adapters were added by PCR enrichment of ligated fragments with Phusion High-Fidelity DNA Polymerase using 10-12 cycles (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Amplified scFv PCR fragments were purified with Gel Extraction kit (Qiaquick Gel Extraction Kit, Cat# 28706, QIAGEN), and subjected to SfiI (Fast Digest SfiI ...
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bioRxiv - Molecular Biology 2021Quote: ... The fragments were amplified by PCR and purified using Qiagen MinElute PCR Purification Kit (Qiagen, Maryland, USA). The final library quality was controlled using the Agilent Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... to all fragments with A-overhangs and DNA was purified using MinElute PCR Purification Kit (QIAGEN #28006). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA fragments were separated using 0.8% agarose gel and then purified using Qiaquick gel extraction kit (Qiagen). To incorporate biotin at the 5’ end of OriLyt DNA ...
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bioRxiv - Microbiology 2023Quote: ... The amplified fragment was digested with BamHI and SmaI restriction enzymes and ligated in pQE80 vector (Qiagen) digested with the same enzymes ...
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bioRxiv - Microbiology 2023Quote: ... This fragment was then ligated in the SacI and SmaI digested pQE80 vector (Qiagen GmbH, Hilden, Germany) using the Quick Ligase kit (New England Biolabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated from cell pellets and tumour fragments following the kit manufacturer’s protocol (Qiagen RNeasy, # 74104). 500 ng RNA was used for cDNA synthesis (SuperScript VILO MasterMix ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pH 5.8) under long day condtions (16h light) at 24 °C (day) 22 °C (night) using the DNeasy Plant Kit (Qiagen). ONSEN copy numbers were determined by qPCR using 12 ng total DNA using the KAPA SYBR FAST master mix universal on a C1000 Touch (Bio-Rad ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... The temperature was increased from 25 °C to 95 °C at 3 °C per minute and fluorescence was measured at 610 nm in a Rotor-Gene Q thermocycler (Qiagen). The experiment was performed in technical triplicates ...
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bioRxiv - Molecular Biology 2019Quote: ... were frozen at −80°C and subsequently digested at 56°C overnight by Proteinase K (10mM in Tris-HCl, 19133, Qiagen). To account for potentially differential cell proliferation rate between treatments all the ATP quantifications were normalized to DNA content in the same samples ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNAs were purified from 3 mL overnight cultures grew at optimum temperature (30 °C or 37 °C) by Dneasy Blood & Tissue Kits (QIAGEN). Additionally ...
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bioRxiv - Genomics 2023Quote: ... The bead-bound gDNA was isothermally amplified for 3 hours at 30 °C then 10 minutes at 65 °C using a miniaturised (1/5 vols) Repli-g Single-Cell assay (Qiagen). The amplified gDNA was cleaned up with 0.8 × vols Ampure XP and 80 % ethanol ...
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bioRxiv - Microbiology 2023Quote: ... Eluates were then treated with Proteinase K (45°C, 1400rpm, 4h) and RNaseA (37°C, 1400rpm, 30min) before cleanup using MiniElute PCR Purification kit (Qiagen). Purified DNA together with input samples before immunoprecipitation were amplified using qPCR SyBr green mix (PCR Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was purified with agarose beads that bind specifically to the His-tag (Ni-NTA Agarose, Qiagen) following the purification hybrid method from the ProBond purification system (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... and the recombinant protein was purified by gravity-flow chromatography using a nickel-charged resin Ni-NTA Agarose (QIAgen) equilibrated with 10 mM imidazole in the lysis buffer ...